Restriction map for
pFN21AB5179
(Circular) (Six-base) MAPSORT of: pFN21AB5179.seq Check: 8256 from: 1 to: 5890
pFN21AB5179 5890 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2874 3233 1937
Size: 937 359 4594
Fragments arranged by size:
4594 937 359
Acc65I G'GTAC_C
Cuts at: 3011 3211 3011
Size: 200 5690
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 3122 3953 5337 5605 1632
Size: 494 996 831 1384 268 1917
Fragments arranged by size:
1917 1384 996 831 494 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 4721 4721
Size: 5890
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5890
AlwNI CAG_nnn'CTG
Cuts at: 3633 5205 3633
Size: 1572 4318
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 3058 3199 3425 3577 5686 1493
Size: 1565 141 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152 141
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BamHI G'GATC_C
Cuts at: 2149 3220 3587 2149
Size: 1071 367 4452
Fragments arranged by size:
4452 1071 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2843 3011 3211 4666 618
Size: 325 217 627 1056 168 200 1455 1842
Fragments arranged by size:
1842 1455 1056 627 325 217 200 168
BanII G_rGCy'C
Cuts at: 1391 3209 1391
Size: 1818 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 928
Size: 971 495 4424
Fragments arranged by size:
4424 971 495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2713 3782 4992 2713
Size: 1069 1210 3611
Fragments arranged by size:
3611 1210 1069
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2495 2514 4501
Size: 107 122 71 631 605 823 19 1987
Cuts at: 4501 136
Size: 1525
Fragments arranged by size:
1987 1525 823 631 605 122 107 71
19
BglII A'GATC_T
Cuts at: 5885 5885
Size: 5890
BlpI GC'TnA_GC
Cuts at: 3307 3307
Size: 5890
Bme1580I G_kGCm'C
Cuts at: 1790 2054 3174 3951 5107 1790
Size: 264 1120 777 1156 2573
Fragments arranged by size:
2573 1156 1120 777 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3621 4579 442
Size: 416 53 83 399 24 2204 958 1753
Fragments arranged by size:
2204 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2421 4550 1239
Size: 1182 2129 2579
Fragments arranged by size:
2579 2129 1182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2343 2535 4015 4880 5178 2343
Size: 192 1480 865 298 3055
Fragments arranged by size:
3055 1480 865 298 192
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2397 2962 4553 882
Size: 617 898 565 1591 2219
Fragments arranged by size:
2219 1591 898 617 565
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 3096 5884 3096
Size: 2788 3102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3697 4079 4714 275
Size: 53 83 186 936 2164 382 635 1451
Fragments arranged by size:
2164 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4323 4721 4995 5142 5553 4323
Size: 398 274 147 411 4660
Fragments arranged by size:
4660 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 1178
Size: 30 165 30 924 30 4711
Fragments arranged by size:
4711 924 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2459 2853 1186
Size: 874 399 394 4223
Fragments arranged by size:
4223 874 399 394
BseYI C'CCAG_C
Cuts at: 2207 5093 5589 2207
Size: 2886 496 2508
Fragments arranged by size:
2886 2508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2582 1163
Size: 573 846 4471
Fragments arranged by size:
4471 846 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2878 3340 4101 5129 1540
Size: 275 186 78 799 462 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 462 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2833 3951 4036 5107 2833
Size: 1118 85 1071 3616
Fragments arranged by size:
3616 1118 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2833 3174 3209 3951 4036 5107
Size: 399 264 779 341 35 742 85 1071
Cuts at: 5107 1391
Size: 2174
Fragments arranged by size:
2174 1071 779 742 399 341 264 85
35
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3245 844
Size: 2401 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3777 1394
Size: 429 1954 3507
Fragments arranged by size:
3507 1954 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4534 4721 1700
Size: 110 2724 187 2869
Fragments arranged by size:
2869 2724 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 2030 3950 4962 2030
Size: 1920 1012 2958
Fragments arranged by size:
2958 1920 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2569 794
Size: 1775 4115
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2470 2470
Size: 5890
BstYI r'GATC_y
Cuts at: 1972 2149 3091 3097 3220 3587 3972 3989 5441
Size: 177 942 6 123 367 385 17 1452
Cuts at: 5441 5527 5539 5885 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 942 385 367 346 177 123
86 17 12 6
Bsu36I CC'TnA_GG
Cuts at: 2769 2769
Size: 5890
BtgI C'CryG_G
Cuts at: 513 1065 3161 513
Size: 552 2096 3242
Fragments arranged by size:
3242 2096 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 3406 4200 4220 3406
Size: 794 20 5076
Fragments arranged by size:
5076 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 3337 4226
Size: 54 1425 50 275 18 1306 201 889
Cuts at: 4226 5855 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1306 889 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3337 1537
Size: 275 1525 4090
Fragments arranged by size:
4090 1525 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 3017 3820 1353
Size: 1278 386 803 3423
Fragments arranged by size:
3423 1278 803 386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3059 4477 4851 4997 387
Size: 1064 138 1470 1418 374 146 1280
Fragments arranged by size:
1470 1418 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2421 3122 3953 4550 5337 5605
Size: 393 494 295 701 831 597 787 268
Cuts at: 5605 1239
Size: 1524
Fragments arranged by size:
1524 831 787 701 597 494 393 295
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2144 2714 2144
Size: 570 5320
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FspI TGC'GCA
Cuts at: 4396 4396
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 5037 1678
Size: 3359 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 2784 2816 4531
Size: 32 135 32 755 32 425 32 1715
Cuts at: 4531 4563 4605 4637 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1715 755 425 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2875 3234 3480 669
Size: 988 281 298 639 359 246 3079
Fragments arranged by size:
3079 988 639 359 298 281 246
HindIII A'AGCT_T
Cuts at: 748 3179 748
Size: 2431 3459
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3015 3215 3015
Size: 200 5690
MfeI C'AATT_G
Cuts at: 2610 3489 2610
Size: 879 5011
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5004 5188 1305
Size: 3699 184 2007
Fragments arranged by size:
3699 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3138 10
Size: 54 1425 343 1306 2762
Fragments arranged by size:
2762 1425 1306 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3848 4207 4366 518
Size: 648 2682 359 159 2042
Fragments arranged by size:
2682 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2885 3306 3983 5131 5376 1606
Size: 1279 421 677 1148 245 2120
Fragments arranged by size:
2120 1279 1148 677 421 245
NcoI C'CATG_G
Cuts at: 513 1065 3161 513
Size: 552 2096 3242
Fragments arranged by size:
3242 2096 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 3248 4793 1132
Size: 97 2019 1545 2229
Fragments arranged by size:
2229 2019 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3074 1348
Size: 1726 4164
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 1108
Size: 1140 4750
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PstI C_TGCA'G
Cuts at: 830 3130 3242 830
Size: 2300 112 3478
Fragments arranged by size:
3478 2300 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
PvuII CAG'CTG
Cuts at: 2885 2885
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 2873 3232 1936
Size: 937 359 4594
Fragments arranged by size:
4594 937 359
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2735 3126 3238 4373 5054 5245 826
Size: 219 1690 391 112 1135 681 191 1471
Fragments arranged by size:
1690 1471 1135 681 391 219 191 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2358 2550 3994 4895 5157 820
Size: 197 926 415 192 1444 901 262 1553
Fragments arranged by size:
1553 1444 926 901 415 262 197 192
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 3248 1132
Size: 2116 3774
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2331 2525 2868 3161 3329 513
Size: 552 1266 194 343 293 168 3074
Fragments arranged by size:
3074 1266 552 343 293 194 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2065 2106 2893 3917 3934 4087 4272
Size: 1255 485 41 787 1024 17 153 185
Cuts at: 4272 4717 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1024 787 485 445 185 153
41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4136 5848 96
Size: 274 80 33 51 1443 2159 1712 138
Fragments arranged by size:
2159 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5850 5882 5850
Size: 32 5858
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5890
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 3059 4019 3059
Size: 960 4930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI