Restriction map for
pFN21AB5178
(Circular) (Six-base) MAPSORT of: pFN21AB5178.seq Check: 1530 from: 1 to: 6160
pFN21AB5178 6160 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2090 2090
Size: 6160
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 3503 1937
Size: 1392 174 4594
Fragments arranged by size:
4594 1392 174
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 4223 5607 5875 1632
Size: 1049 1542 1384 268 1917
Fragments arranged by size:
1917 1542 1384 1049 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 5059 6036 1203
Size: 3856 977 1327
Fragments arranged by size:
3856 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 2627 2924 2970 1166
Size: 1461 297 46 4356
Fragments arranged by size:
4356 1461 297 46
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 2504
Size: 32 6128
AlwNI CAG_nnn'CTG
Cuts at: 2572 2614 2683 2930 3187 3903 5475 2572
Size: 42 69 247 257 716 1572 3257
Fragments arranged by size:
3257 1572 716 257 247 69 42
ApaLI G'TGCA_C
Cuts at: 4217 5373 4217
Size: 1156 5004
ApoI r'AATT_y
Cuts at: 1493 3469 3695 3847 5956 1493
Size: 1976 226 152 2109 1697
Fragments arranged by size:
2109 1976 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 3485 1870
Size: 73 1542 4545
Fragments arranged by size:
4545 1542 73
BamHI G'GATC_C
Cuts at: 3490 3857 3490
Size: 367 5793
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2426 2954 3481 4936 618
Size: 325 217 627 639 528 527 1455 1842
Fragments arranged by size:
1842 1455 639 627 528 527 325 217
BanII G_rGCy'C
Cuts at: 1391 2895 3321 3479 1391
Size: 1504 426 158 4072
Fragments arranged by size:
4072 1504 426 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 2795 928
Size: 971 405 491 4293
Fragments arranged by size:
4293 971 491 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4351 4385 1903
Size: 34 50 34 2330 34 3678
Fragments arranged by size:
3678 2330 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 3400 1548
Size: 1852 4308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 4771 136
Size: 107 122 71 631 605 886 2213 1525
Fragments arranged by size:
2213 1525 886 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3201 3449 3577 3201
Size: 248 128 5784
Fragments arranged by size:
5784 248 128
Bme1580I G_kGCm'C
Cuts at: 1790 2957 3009 4221 5377 1790
Size: 1167 52 1212 1156 2573
Fragments arranged by size:
2573 1212 1167 1156 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2017 3891 4849
Size: 416 53 83 399 24 600 1874 958
Cuts at: 4849 442
Size: 1753
Fragments arranged by size:
1874 1753 958 600 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2989 4820 1239
Size: 1750 1831 2579
Fragments arranged by size:
2579 1831 1750
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2985 4285 5150 5448 2985
Size: 1300 865 298 3697
Fragments arranged by size:
3697 1300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3083 4823 882
Size: 617 1584 1740 2219
Fragments arranged by size:
2219 1740 1584 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3967 4349 4984 275
Size: 53 83 186 936 2434 382 635 1451
Fragments arranged by size:
2434 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4593 4991 5265 5412 5823 4593
Size: 398 274 147 411 4930
Fragments arranged by size:
4930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 2802 2832 1178
Size: 30 165 30 1101 30 268 30 4506
Fragments arranged by size:
4506 1101 268 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6160
BseYI C'CCAG_C
Cuts at: 2607 5363 5859 2607
Size: 2756 496 2908
Fragments arranged by size:
2908 2756 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2500 2639 2696 3203 3404 1163
Size: 573 764 139 57 507 201 3919
Fragments arranged by size:
3919 764 573 507 201 139 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2020 3455 4221 4306 5377 2020
Size: 1435 766 85 1071 2803
Fragments arranged by size:
2803 1435 1071 766 85
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2895 2957 3009 3321 3455 3479
Size: 399 230 875 62 52 312 134 24
Cuts at: 3479 4221 4306 5377 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 875 742 399 312 230 134
85 62 52 24
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 4049 5779 4049
Size: 1730 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2090 2218 3186 3195 3243 3515 844
Size: 1246 128 968 9 48 272 3489
Fragments arranged by size:
3489 1246 968 272 128 48 9
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 3256 4655 4829 65
Size: 1183 1074 934 1399 174 1396
Fragments arranged by size:
1399 1396 1183 1074 934 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 4804 4991 1700
Size: 110 232 2762 187 2869
Fragments arranged by size:
2869 2762 232 187 110
BsrGI T'GTAC_A
Cuts at: 96 2112 2563 96
Size: 2016 451 3693
Fragments arranged by size:
3693 2016 451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3187 794
Size: 2393 3767
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 2102 3364 1184
Size: 918 1262 3980
Fragments arranged by size:
3980 1262 918
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2629 2341
Size: 288 5872
BstYI r'GATC_y
Cuts at: 1972 3167 3490 3857 4242 4259 5711 5797 5809
Size: 1195 323 367 385 17 1452 86 12
Cuts at: 5809 6155 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1195 385 367 346 323 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2054 3351 2054
Size: 1297 4863
BtgI C'CryG_G
Cuts at: 513 1065 2389 2622 513
Size: 552 1324 233 4051
Fragments arranged by size:
4051 1324 552 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 3411 3676 4470 4490 3411
Size: 265 794 20 5081
Fragments arranged by size:
5081 794 265 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 2886 3438
Size: 54 1425 50 275 18 501 555 552
Cuts at: 3438 3607 4496 6125 8
Size: 169 889 1629 43
Fragments arranged by size:
1629 1425 889 555 552 501 275 169
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4090 1353
Size: 2737 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 2989 4223 4820 5607 5875 1239
Size: 393 1049 308 1234 597 787 268 1524
Fragments arranged by size:
1524 1234 1049 787 597 393 308 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3065 3065
Size: 6160
EcoO109I rG'GnC_Cy
Cuts at: 2251 2542 2569 2680 3184 2251
Size: 291 27 111 504 5227
Fragments arranged by size:
5227 504 291 111 27
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FspI TGC'GCA
Cuts at: 2535 4666 2535
Size: 2131 4029
FspAI rTGC'GCAy
Cuts at: 2535 2535
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 3024 3411 5307 1678
Size: 1346 387 1896 2531
Fragments arranged by size:
2531 1896 1346 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2975 3007 3028 3060 4801
Size: 32 135 32 1403 32 21 32 1741
Cuts at: 4801 4833 4875 4907 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1741 1403 135 42 32 32 32
32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3504 3750 669
Size: 988 281 1566 246 3079
Fragments arranged by size:
3079 1566 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6160
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 3440 10
Size: 54 1425 343 501 1107 2730
Fragments arranged by size:
2730 1425 1107 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 2924 2970 3263 4118 4477 4636
Size: 648 1461 297 46 293 855 359 159
Cuts at: 4636 518
Size: 2042
Fragments arranged by size:
2042 1461 855 648 359 297 293 159
46
MspA1I CmG'CkG
Cuts at: 1606 2164 2266 2468 2611 3448 3576 4253 5401
Size: 558 102 202 143 837 128 677 1148
Cuts at: 5401 5646 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 837 677 558 245 202 143
128 102
NcoI C'CATG_G
Cuts at: 513 1065 2389 513
Size: 552 1324 4284
Fragments arranged by size:
4284 1324 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 2365 3518 5063 1132
Size: 97 1136 1153 1545 2229
Fragments arranged by size:
2229 1545 1153 1136 97
PasI CC'CwG_GG
Cuts at: 1348 2584 1348
Size: 1236 4924
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 2979 2979
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2504 2536 4229 4261 5742 5774 2504
Size: 32 1693 32 1481 32 2890
Fragments arranged by size:
2890 1693 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 3184 2542
Size: 642 5518
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PstI C_TGCA'G
Cuts at: 830 2101 2559 3183 3512 830
Size: 1271 458 624 329 3478
Fragments arranged by size:
3478 1271 624 458 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2164 2266 2468 2611 2164
Size: 102 202 143 5713
Fragments arranged by size:
5713 202 143 102
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SbfI CC_TGCA'GG
Cuts at: 2559 3183 3512 2559
Size: 624 329 5207
Fragments arranged by size:
5207 624 329
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2097 2255 2477 2555 3179 3508 4643
Size: 219 1052 158 222 78 624 329 1135
Cuts at: 4643 5324 5515 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1052 681 624 329 222 219
191 158 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3000 4264 5165 5427 820
Size: 197 926 1057 1264 901 262 1553
Fragments arranged by size:
1553 1264 1057 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 2365 3518 1132
Size: 1233 1153 3774
Fragments arranged by size:
3774 1233 1153
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 2652 1517
Size: 1135 5025
StyI C'CwwG_G
Cuts at: 513 1065 2389 2454 2498 2857 3599 513
Size: 552 1324 65 44 359 742 3074
Fragments arranged by size:
3074 1324 742 552 359 65 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2480 2896 4187 4204 4357 4542 4987
Size: 1255 900 416 1291 17 153 185 445
Cuts at: 4987 325
Size: 1498
Fragments arranged by size:
1498 1291 1255 900 445 416 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2563 4406
Size: 274 80 33 51 1443 135 451 1843
Cuts at: 4406 6118 96
Size: 1712 138
Fragments arranged by size:
1843 1712 1443 451 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2802 2834 6120 6152 2802
Size: 32 3286 32 2810
Fragments arranged by size:
3286 2810 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3151 3151
Size: 6160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 2873 4289 2873
Size: 1416 4744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI