Restriction map for
pFN21AB5169
(Circular) (Six-base) MAPSORT of: pFN21AB5169.seq Check: 5404 from: 1 to: 6016
pFN21AB5169 6016 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2409 3359 1937
Size: 472 950 4594
Fragments arranged by size:
4594 950 472
Acc65I G'GTAC_C
Cuts at: 2597 3337 2597
Size: 740 5276
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4079 5463 5731 1632
Size: 2447 1384 268 1917
Fragments arranged by size:
2447 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 3240 4915 5892 1203
Size: 2037 1675 977 1327
Fragments arranged by size:
2037 1675 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 4745 4745
Size: 6016
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2017 3759 5331 2017
Size: 1742 1572 2702
Fragments arranged by size:
2702 1742 1572
ApaI G_GGCC'C
Cuts at: 2902 2902
Size: 6016
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3325 3551 3703 5812 1493
Size: 1832 226 152 2109 1697
Fragments arranged by size:
2109 1832 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2190 2781 3008 3180 3341 1870
Size: 73 214 33 591 227 172 161 4545
Fragments arranged by size:
4545 591 227 214 172 161 73 33
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2597 3096 3337 4792 618
Size: 325 217 627 810 499 241 1455 1842
Fragments arranged by size:
1842 1455 810 627 499 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2902 2961 3009 3335 1391
Size: 1511 59 48 326 4072
Fragments arranged by size:
4072 1511 326 59 48
BbeI G_GCGC'C
Cuts at: 3100 3100
Size: 6016
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5045
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2575 4627 136
Size: 107 122 71 631 605 903 2052 1525
Fragments arranged by size:
2052 1525 903 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 3433 3433
Size: 6016
Bme1580I G_kGCm'C
Cuts at: 1790 2902 4077 5233 1790
Size: 1112 1175 1156 2573
Fragments arranged by size:
2573 1175 1156 1112
BmgBI CAC'GTC
Cuts at: 1318 2536 1318
Size: 1218 4798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2005 3747 4705
Size: 416 53 83 399 24 588 1742 958
Cuts at: 4705 442
Size: 1753
Fragments arranged by size:
1753 1742 958 588 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2606
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2584 2818 2982 3233 4676 1239
Size: 860 485 234 164 251 1443 2579
Fragments arranged by size:
2579 1443 860 485 251 234 164
Bpu10I CC'TnA_GC
Cuts at: 2295 2295
Size: 6016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4141 5006 5304 4141
Size: 865 298 4853
Fragments arranged by size:
4853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4679 882
Size: 617 3180 2219
Fragments arranged by size:
3180 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3807 493
Size: 711 2603 2702
Fragments arranged by size:
2702 2603 711
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3097 3823 4205 4840
Size: 53 83 186 936 1564 726 382 635
Cuts at: 4840 275
Size: 1451
Fragments arranged by size:
1564 1451 936 726 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2361 2865 3078 3280 4449 4847 5121 5268 5679
Size: 504 213 202 1169 398 274 147 411
Cuts at: 5679 2361
Size: 2698
Fragments arranged by size:
2698 1169 504 411 398 274 213 202
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 3275 3305 1178
Size: 30 165 30 1059 30 783 30 3889
Fragments arranged by size:
3889 1059 783 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2160 2210 2598 2622 2834 2939 1186
Size: 974 50 388 24 212 105 4263
Fragments arranged by size:
4263 974 388 212 105 50 24
BseYI C'CCAG_C
Cuts at: 5219 5715 5219
Size: 496 5520
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3009 3138 4077 4162 5233 3009
Size: 129 939 85 1071 3792
Fragments arranged by size:
3792 1071 939 129 85
BsmI GAATG_Cn'
Cuts at: 2632 3527 3620 2632
Size: 895 93 5028
Fragments arranged by size:
5028 895 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2902 2961 3009 3138 3335 4077 4162
Size: 399 1112 59 48 129 197 742 85
Cuts at: 4162 5233 1391
Size: 1071 2174
Fragments arranged by size:
2174 1112 1071 742 399 197 129 85
59 48
BspEI T'CCGG_A
Cuts at: 2361 3280 5679 2361
Size: 919 2399 2698
Fragments arranged by size:
2698 2399 919
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 3371 844
Size: 1232 1295 3489
Fragments arranged by size:
3489 1295 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 2719 2734 2778 2824 2842 2896
Size: 429 410 486 15 44 46 18 54
Cuts at: 2896 2911 3094 3151 3163 3903 1394
Size: 15 183 57 12 740 3507
Fragments arranged by size:
3507 740 486 429 410 183 57 54
46 44 18 15 15 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 2124 4511 4685 65
Size: 1183 876 2387 174 1396
Fragments arranged by size:
2387 1396 1183 876 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2787 4660 4847 1700
Size: 110 977 1873 187 2869
Fragments arranged by size:
2869 1873 977 187 110
BsrGI T'GTAC_A
Cuts at: 96 3243 96
Size: 3147 2869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 2178 2462 4076 5088 2178
Size: 284 1614 1012 3106
Fragments arranged by size:
3106 1614 1012 284
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6016
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 3346 3713 4098 4115 5567 5653 5665 6011
Size: 1374 367 385 17 1452 86 12 346
Cuts at: 6011 1972
Size: 1977
Fragments arranged by size:
1977 1452 1374 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2434 513
Size: 552 1369 4095
Fragments arranged by size:
4095 1369 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2658 487
Size: 683 56 1432 3845
Fragments arranged by size:
3845 1432 683 56
BtsI GCAGTG_nn'
Cuts at: 3163 3532 4326 4346 3163
Size: 369 794 20 4833
Fragments arranged by size:
4833 794 369 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 3463 4352
Size: 54 1425 50 275 18 1366 267 889
Cuts at: 4352 5981 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1366 889 275 267 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 2599 3946 1353
Size: 971 275 1347 3423
Fragments arranged by size:
3423 1347 971 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2806 2969 3085 4603 4977 5123
Size: 1064 138 1217 163 116 1518 374 146
Cuts at: 5123 387
Size: 1280
Fragments arranged by size:
1518 1280 1217 1064 374 163 146 138
116
EcoICRI GAG'CTC
Cuts at: 3007 3007
Size: 6016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2584 2818 2982 3233 4079 4676
Size: 393 467 485 234 164 251 846 597
Cuts at: 4676 5463 5731 1239
Size: 787 268 1524
Fragments arranged by size:
1524 846 787 597 485 467 393 268
251 234 164
EcoO109I rG'GnC_Cy
Cuts at: 2152 2272 2524 2568 2152
Size: 120 252 44 5600
Fragments arranged by size:
5600 252 120 44
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 6016
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2800 2833 3100 5163 1678
Size: 1122 33 267 2063 2531
Fragments arranged by size:
2531 2063 1122 267 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2636 2668 4657
Size: 32 135 32 890 32 142 32 1989
Cuts at: 4657 4689 4731 4763 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1989 890 142 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3360 3606 669
Size: 988 281 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KasI G'GCGC_C
Cuts at: 3096 3096
Size: 6016
KpnI G_GTAC'C
Cuts at: 2601 3341 2601
Size: 740 5276
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 5130 5314 1305
Size: 893 2932 184 2007
Fragments arranged by size:
2932 2007 893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3198 10
Size: 54 1425 343 1366 2828
Fragments arranged by size:
2828 1425 1366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 3245 3974 4333 4492 518
Size: 648 1330 749 729 359 159 2042
Fragments arranged by size:
2042 1330 749 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3432 4109 5257 5502 1606
Size: 1826 677 1148 245 2120
Fragments arranged by size:
2120 1826 1148 677 245
NarI GG'CG_CC
Cuts at: 3097 3097
Size: 6016
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5464
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NruI TCG'CGA
Cuts at: 2811 2811
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 2476 3244 3374 4919 1132
Size: 97 1247 768 130 1545 2229
Fragments arranged by size:
2229 1545 1247 768 130 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 3240 4915 3240
Size: 1675 4341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2272 2524 2152
Size: 120 252 5644
Fragments arranged by size:
5644 252 120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PspOMI G'GGCC_C
Cuts at: 2898 2898
Size: 6016
PstI C_TGCA'G
Cuts at: 830 3368 830
Size: 2538 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
SacI G_AGCT'C
Cuts at: 3009 3009
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SanDI GG'GwC_CC
Cuts at: 2272 2272
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2410 3364 4499 5180 5371 826
Size: 219 1365 954 1135 681 191 1471
Fragments arranged by size:
1471 1365 1135 954 681 219 191
SfoI GGC'GCC
Cuts at: 3098 3098
Size: 6016
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 2783 3343 1872
Size: 911 560 4545
Fragments arranged by size:
4545 911 560
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2157 4120 5021 5283 820
Size: 197 926 214 1963 901 262 1553
Fragments arranged by size:
1963 1553 926 901 262 214 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 4944
StyI C'CwwG_G
Cuts at: 513 1065 3455 513
Size: 552 2390 3074
Fragments arranged by size:
3074 2390 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4043 4060 4213 4398 4843 325
Size: 1255 2463 17 153 185 445 1498
Fragments arranged by size:
2463 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3243 4262 5974
Size: 274 80 33 51 1443 1266 1019 1712
Cuts at: 5974 96
Size: 138
Fragments arranged by size:
1712 1443 1266 1019 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5976 6008 5976
Size: 32 5984
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6016
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 2157 1943
Size: 214 5802
XmaI C'CCGG_G
Cuts at: 1870 2781 3341 1870
Size: 911 560 4545
Fragments arranged by size:
4545 911 560
XmnI GAAnn'nnTTC
Cuts at: 4145 4145
Size: 6016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI