Restriction map for
pFN21AB5155
(Circular) (Six-base) MAPSORT of: pFN21AB5155.seq Check: 2475 from: 1 to: 6238
pFN21AB5155 6238 bp
With 182 enzymes: SgfI *
February 27, 2009 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 3581 1937
Size: 1542 102 4594
Fragments arranged by size:
4594 1542 102
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4301 5685 5953 1632
Size: 2669 1384 268 1917
Fragments arranged by size:
2669 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 2750 3426 1166
Size: 1584 676 3978
Fragments arranged by size:
3978 1584 676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 6206
AlwNI CAG_nnn'CTG
Cuts at: 3981 5553 3981
Size: 1572 4666
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 3230 3547 3773 3925 6034 1493
Size: 1737 317 226 152 2109 1697
Fragments arranged by size:
2109 1737 1697 317 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 1870
Size: 73 1620 4545
Fragments arranged by size:
4545 1620 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3401 3559 5014 618
Size: 325 217 627 1614 158 1455 1842
Fragments arranged by size:
1842 1614 1455 627 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2061 2581 3557 1391
Size: 670 520 976 4072
Fragments arranged by size:
4072 976 670 520
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5267
BbvCI CC'TCA_GC
Cuts at: 2852 2852
Size: 6238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 4849 136
Size: 107 122 71 631 605 502 2675 1525
Fragments arranged by size:
2675 1525 631 605 502 122 107 71
BglII A'GATC_T
Cuts at: 2438 3307 6233 2438
Size: 869 2926 2443
Fragments arranged by size:
2926 2443 869
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 4299 5455 1790
Size: 2509 1156 2573
Fragments arranged by size:
2573 2509 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 2748 3969
Size: 416 53 83 399 24 609 722 1221
Cuts at: 3969 4927 442
Size: 958 1753
Fragments arranged by size:
1753 1221 958 722 609 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 4898 1239
Size: 1415 2244 2579
Fragments arranged by size:
2579 2244 1415
Bpu10I CC'TnA_GC
Cuts at: 2852 2852
Size: 6238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4901 882
Size: 617 3402 2219
Fragments arranged by size:
3402 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4045 4427 5062 275
Size: 53 83 186 936 2512 382 635 1451
Fragments arranged by size:
2512 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2244 2274 3193 3223 1178
Size: 30 165 30 841 30 919 30 4193
Fragments arranged by size:
4193 919 841 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2006 2265 3426 1186
Size: 820 259 1161 3998
Fragments arranged by size:
3998 1161 820 259
BseYI C'CCAG_C
Cuts at: 2053 2094 5441 5937 2053
Size: 41 3347 496 2354
Fragments arranged by size:
3347 2354 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 1163
Size: 573 1062 4603
Fragments arranged by size:
4603 1062 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2581 2747 4299 4384 5455 2581
Size: 166 1552 85 1071 3364
Fragments arranged by size:
3364 1552 1071 166 85
BsmI GAATG_Cn'
Cuts at: 2498 3749 3842 2498
Size: 1251 93 4894
Fragments arranged by size:
4894 1251 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2581 2747 3557 4299 4384 5455
Size: 399 271 520 166 810 742 85 1071
Cuts at: 5455 1391
Size: 2174
Fragments arranged by size:
2174 1071 810 742 520 399 271 166
85
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2733 3593 844
Size: 1889 860 3489
Fragments arranged by size:
3489 1889 860
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2706 3186 4733 4907 65
Size: 1183 1458 480 1547 174 1396
Fragments arranged by size:
1547 1458 1396 1183 480 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4882 5069 1700
Size: 110 3072 187 2869
Fragments arranged by size:
3072 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 3310 2632
Size: 678 5560
BstYI r'GATC_y
Cuts at: 1972 2292 2438 2988 3307 3568 3935 4320 4337
Size: 320 146 550 319 261 367 385 17
Cuts at: 4337 5789 5875 5887 6233 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 550 385 367 346 320 319
261 146 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3005 513
Size: 552 1940 3746
Fragments arranged by size:
3746 1940 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2289 2524 487
Size: 683 56 1063 235 4201
Fragments arranged by size:
4201 1063 683 235 56
BtsI GCAGTG_nn'
Cuts at: 2468 2523 2913 3057 3078 3454 3754 4548 4568
Size: 55 390 144 21 376 300 794 20
Cuts at: 4568 2468
Size: 4138
Fragments arranged by size:
4138 794 390 376 300 144 55 21
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3229 3542 3229
Size: 313 5925
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2443 2703 2899
Size: 54 1425 50 275 18 613 260 196
Cuts at: 2899 3172 3685 4574 6203 8
Size: 273 513 889 1629 43
Fragments arranged by size:
1629 1425 889 613 513 275 273 260
196 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2882 3240 4168 1353
Size: 1529 358 928 3423
Fragments arranged by size:
3423 1529 928 358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2579 2579
Size: 6238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2654 4301 4898 5685 5953 1239
Size: 393 1022 1647 597 787 268 1524
Fragments arranged by size:
1647 1524 1022 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2417 3010 2417
Size: 593 5645
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
EcoRV GAT'ATC
Cuts at: 2377 2377
Size: 6238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3498 3498
Size: 6238
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 5385 1678
Size: 3707 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2406 2438 3193 3225 4879
Size: 32 135 32 834 32 755 32 1654
Cuts at: 4879 4911 4953 4985 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1654 834 755 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3370 3582 3828 669
Size: 988 281 1432 212 246 3079
Fragments arranged by size:
3079 1432 988 281 246 212
HindIII A'AGCT_T
Cuts at: 748 2308 748
Size: 1560 4678
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 3156 3837 3156
Size: 681 5557
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 2464 2507 2606 2784 3401 5352 5536
Size: 880 279 43 99 178 617 1951 184
Cuts at: 5536 1305
Size: 2007
Fragments arranged by size:
2007 1951 880 617 279 184 178 99
43
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2445 2705 2901 3174 10
Size: 54 1425 343 613 260 196 273 3074
Fragments arranged by size:
3074 1425 613 343 273 260 196 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 2600 2699 2750 3426 4196 4555
Size: 648 1377 57 99 51 676 770 359
Cuts at: 4555 4714 518
Size: 159 2042
Fragments arranged by size:
2042 1377 770 676 648 359 159 99
57 51
MspA1I CmG'CkG
Cuts at: 1606 2897 3654 4331 5479 5724 1606
Size: 1291 757 677 1148 245 2120
Fragments arranged by size:
2120 1291 1148 757 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3005 513
Size: 552 1940 3746
Fragments arranged by size:
3746 1940 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3061 3596 5141 1132
Size: 97 1832 535 1545 2229
Fragments arranged by size:
2229 1832 1545 535 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3383 1108
Size: 2275 3963
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2417 3010 2417
Size: 593 5645
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3590 830
Size: 2760 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 2897 2897
Size: 6238
SacI G_AGCT'C
Cuts at: 2581 2581
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SanDI GG'GwC_CC
Cuts at: 3010 3010
Size: 6238
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 2301 4486 6198 2301
Size: 2185 1712 2341
Fragments arranged by size:
2341 2185 1712
SfcI C'TryA_G
Cuts at: 826 1045 3496 3586 4721 5402 5593 826
Size: 219 2451 90 1135 681 191 1471
Fragments arranged by size:
2451 1471 1135 681 219 191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4342 5243 5505 820
Size: 197 926 2399 901 262 1553
Fragments arranged by size:
2399 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 2478 2978 1517
Size: 961 500 4777
Fragments arranged by size:
4777 961 500
StyI C'CwwG_G
Cuts at: 513 1065 2625 3005 3520 3677 513
Size: 552 1560 380 515 157 3074
Fragments arranged by size:
3074 1560 552 515 380 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2342 4265 4282 4435 4620 5065 325
Size: 1255 762 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 762 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2299 3465 3485
Size: 274 80 33 51 1443 322 1166 20
Cuts at: 3485 4484 6196 96
Size: 999 1712 138
Fragments arranged by size:
1712 1443 1166 999 322 274 138 80
51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2298 2330 2748 2780 3167 3199 6198 6230 2298
Size: 32 418 32 387 32 2999 32 2306
Fragments arranged by size:
2999 2306 418 387 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI