Restriction map for
pFN21AB5144
(Circular) (Six-base) MAPSORT of: pFN21AB5144.seq Check: 3144 from: 1 to: 6895
pFN21AB5144 6895 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4705 278
Size: 53 83 186 936 3169 2468
Fragments arranged by size:
3169 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4238 1937
Size: 2301 4594
Acc65I G'GTAC_C
Cuts at: 4216 4216
Size: 6895
AclI AA'CG_TT
Cuts at: 1563 5022 5395 1563
Size: 3459 373 3063
Fragments arranged by size:
3459 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2190 3849 4958 6342 6610 1632
Size: 558 1659 1109 1384 268 1917
Fragments arranged by size:
1917 1659 1384 1109 558 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6698
AflIII A'CryG_T
Cuts at: 1203 2856 5794 6771 1203
Size: 1653 2938 977 1327
Fragments arranged by size:
2938 1653 1327 977
AgeI A'CCGG_T
Cuts at: 5726 5726
Size: 6895
AhdI GACnn_n'nnGTC
Cuts at: 5624 5624
Size: 6895
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6895
AlwNI CAG_nnn'CTG
Cuts at: 3905 4187 4638 6210 3905
Size: 282 451 1572 4590
Fragments arranged by size:
4590 1572 451 282
ApaLI G'TGCA_C
Cuts at: 4952 6108 4952
Size: 1156 5739
ApoI r'AATT_y
Cuts at: 1493 2376 3115 3134 3489 3833 4069 4204 4430
Size: 883 739 19 355 344 236 135 226
Cuts at: 4430 4582 6691 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 883 739 355 344 236 226
152 135 19
AseI AT'TA_AT
Cuts at: 160 1032 5449 160
Size: 872 4417 1606
Fragments arranged by size:
4417 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
AvaI C'yCGr_G
Cuts at: 1870 1943 4220 1870
Size: 73 2277 4545
Fragments arranged by size:
4545 2277 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2139 2172 2139
Size: 33 6862
BamHI G'GATC_C
Cuts at: 4103 4225 4592 4103
Size: 122 367 6406
Fragments arranged by size:
6406 367 122
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4216 5671 618
Size: 325 217 627 2429 1455 1842
Fragments arranged by size:
2429 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2892 4214 1391
Size: 1501 1322 4072
Fragments arranged by size:
4072 1501 1322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 2742 3503 3675 928
Size: 971 192 651 761 172 4148
Fragments arranged by size:
4148 971 761 651 192 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4039 4073 5086 5120 1903
Size: 34 50 34 2018 34 1013 34 3678
Fragments arranged by size:
3678 2018 1013 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4111 4787 5997 4111
Size: 676 1210 5009
Fragments arranged by size:
5009 1210 676
BclI T'GATC_A
Cuts at: 1548 2733 1548
Size: 1185 5710
BfrBI ATG'CAT
Cuts at: 3105 3105
Size: 6895
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5506 136
Size: 107 122 71 631 605 3834 1525
Fragments arranged by size:
3834 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6890 6890
Size: 6895
BlpI GC'TnA_GC
Cuts at: 3325 4312 3325
Size: 987 5908
Bme1580I G_kGCm'C
Cuts at: 1790 4956 6112 1790
Size: 3166 1156 2573
Fragments arranged by size:
3166 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6895
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2432 3893 4626
Size: 416 53 83 399 24 1015 1461 733
Cuts at: 4626 5584 442
Size: 958 1753
Fragments arranged by size:
1753 1461 1015 958 733 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2515 2906 2920 5555 1239
Size: 1276 391 14 2635 2579
Fragments arranged by size:
2635 2579 1276 391 14
Bpu10I CC'TnA_GC
Cuts at: 2868 2868
Size: 6895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2901 5020 5885 6183 2901
Size: 2119 865 298 3613
Fragments arranged by size:
3613 2119 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5558 882
Size: 617 4059 2219
Fragments arranged by size:
4059 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4686 493
Size: 711 3482 2702
Fragments arranged by size:
3482 2702 711
BsaBI GATnn'nnATC
Cuts at: 2690 6889 2690
Size: 4199 2696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4702 5084 5719 275
Size: 53 83 186 936 3169 382 635 1451
Fragments arranged by size:
3169 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5328 5726 6000 6147 6558 5328
Size: 398 274 147 411 5665
Fragments arranged by size:
5665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6670
Fragments arranged by size:
6670 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3726 1186
Size: 2540 4355
BseYI C'CCAG_C
Cuts at: 3685 3698 6098 6594 3685
Size: 13 2400 496 3986
Fragments arranged by size:
3986 2400 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4345 5106 6134 1540
Size: 275 186 2344 761 1028 2301
Fragments arranged by size:
2344 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4956 5041 6112 4956
Size: 85 1071 5739
Fragments arranged by size:
5739 1071 85
BsmI GAATG_Cn'
Cuts at: 2742 2819 2847 4406 4499 2742
Size: 77 28 1559 93 5138
Fragments arranged by size:
5138 1559 93 77 28
BsmBI CGTCTCn'nnnn_
Cuts at: 3116 3116
Size: 6895
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2892 4214 4956 5041 6112 1391
Size: 399 1102 1322 742 85 1071 2174
Fragments arranged by size:
2174 1322 1102 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6558 6558
Size: 6895
BspHI T'CATG_A
Cuts at: 3129 4784 6514 3129
Size: 1655 1730 3510
Fragments arranged by size:
3510 1730 1655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4250 844
Size: 3406 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4782 1394
Size: 429 2959 3507
Fragments arranged by size:
3507 2959 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2955 3752 5390 5564 65
Size: 1183 1707 797 1638 174 1396
Fragments arranged by size:
1707 1638 1396 1183 797 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3320 5539 5726 1700
Size: 110 1510 2219 187 2869
Fragments arranged by size:
2869 2219 1510 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6895
BssSI C'ACGA_G
Cuts at: 4955 5967 4955
Size: 1012 5883
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2533 794
Size: 1739 5156
BstBI TT'CG_AA
Cuts at: 5699 5699
Size: 6895
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6895
BstYI r'GATC_y
Cuts at: 1972 2165 4103 4225 4592 4977 4994 6446 6532
Size: 193 1938 122 367 385 17 1452 86
Cuts at: 6532 6544 6890 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1938 1452 385 367 346 193 122
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2932 2932
Size: 6895
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3365 4032 487
Size: 683 56 2139 667 3350
Fragments arranged by size:
3350 2139 683 667 56
BtsI GCAGTG_nn'
Cuts at: 3593 4411 5205 5225 3593
Size: 818 794 20 5263
Fragments arranged by size:
5263 818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6895
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3036 3071 529
Size: 35 2472 35 4353
Fragments arranged by size:
4353 2472 35 35
DraI TTT'AAA
Cuts at: 2829 4199 2829
Size: 1370 5525
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5902 809
Size: 507 4586 1802
Fragments arranged by size:
4586 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 4342 5231
Size: 54 1425 50 275 18 301 2211 889
Cuts at: 5231 6860 8
Size: 1629 43
Fragments arranged by size:
2211 1629 1425 889 301 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4342 1537
Size: 275 2530 4090
Fragments arranged by size:
4090 2530 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 3246 3270 3288 4825 1353
Size: 780 1113 24 18 1537 3423
Fragments arranged by size:
3423 1537 1113 780 24 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5482 5856 6002 387
Size: 1064 138 3893 374 146 1280
Fragments arranged by size:
3893 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2515 2906 2920 3849 4958 5555
Size: 393 558 325 391 14 929 1109 597
Cuts at: 5555 6342 6610 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1109 929 787 597 558 393 391
325 268 14
EcoO109I rG'GnC_Cy
Cuts at: 3988 3988
Size: 6895
EcoRI G'AATT_C
Cuts at: 2376 4204 4582 2376
Size: 1828 378 4689
Fragments arranged by size:
4689 1828 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2090 2208 3509 2090
Size: 118 1301 5476
Fragments arranged by size:
5476 1301 118
FspI TGC'GCA
Cuts at: 5401 5401
Size: 6895
HaeII r_GCGC'y
Cuts at: 1678 6042 1678
Size: 4364 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 3809 3841 3972
Size: 32 135 32 585 32 1620 32 131
Cuts at: 3972 4004 4046 4048 4078 4080 5536 5568 5610
Size: 32 42 2 30 2 1456 32 42
Cuts at: 5610 5642 1373
Size: 32 2626
Fragments arranged by size:
2626 1620 1456 585 135 131 42 42
32 32 32 32 32 32 32 30
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4239 4485 669
Size: 988 281 2301 246 3079
Fragments arranged by size:
3079 2301 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 748
Size: 2122 4773
HpaI GTT'AAC
Cuts at: 4485 4485
Size: 6895
KpnI G_GTAC'C
Cuts at: 4220 4220
Size: 6895
MfeI C'AATT_G
Cuts at: 2838 4494 2838
Size: 1656 5239
MluI A'CGCG_T
Cuts at: 6771 6771
Size: 6895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2209 3872 6009 6193 1305
Size: 904 1663 2137 184 2007
Fragments arranged by size:
2137 2007 1663 904 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2133 10
Size: 54 1425 343 301 4772
Fragments arranged by size:
4772 1425 343 301 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2272 3000 4853 5212 5371 518
Size: 648 1106 728 1853 359 159 2042
Fragments arranged by size:
2042 1853 1106 728 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2093 4311 4988 6136 6381 1606
Size: 487 2218 677 1148 245 2120
Fragments arranged by size:
2218 2120 1148 677 487 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6343
NdeI CA'TA_TG
Cuts at: 387 2788 387
Size: 2401 4494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6895
NotI GC'GGCC_GC
Cuts at: 4342 4342
Size: 6895
NsiI A_TGCA'T
Cuts at: 3107 3107
Size: 6895
NspI r_CATG'y
Cuts at: 1132 1229 2860 4253 5798 1132
Size: 97 1631 1393 1545 2229
Fragments arranged by size:
2229 1631 1545 1393 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6895
PciI A'CATG_T
Cuts at: 2856 5794 2856
Size: 2938 3957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2898 4073 1108
Size: 1790 1175 3930
Fragments arranged by size:
3930 1790 1175
PmeI GTTT'AAAC
Cuts at: 4199 4199
Size: 6895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4964 4996 6477 6509 4964
Size: 32 1481 32 5350
Fragments arranged by size:
5350 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3988 3988
Size: 6895
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6895
PsiI TTA'TAA
Cuts at: 4465 4465
Size: 6895
PstI C_TGCA'G
Cuts at: 830 3205 4247 830
Size: 2375 1042 3478
Fragments arranged by size:
3478 2375 1042
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6895
PvuII CAG'CTG
Cuts at: 2093 2093
Size: 6895
SalI G'TCGA_C
Cuts at: 1936 4237 1936
Size: 2301 4594
SapI GCTCTTCn'nnn_
Cuts at: 3270 3288 3270
Size: 18 6877
SbfI CC_TGCA'GG
Cuts at: 4247 4247
Size: 6895
ScaI AGT'ACT
Cuts at: 5143 6855 5143
Size: 1712 5183
SfcI C'TryA_G
Cuts at: 826 1045 2238 3201 3906 4243 5378 6059 6250
Size: 219 1193 963 705 337 1135 681 191
Cuts at: 6250 826
Size: 1471
Fragments arranged by size:
1471 1193 1135 963 705 681 337 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6895
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6895
SmaI CCC'GGG
Cuts at: 1872 4222 1872
Size: 2350 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2916 4999 5900 6162 820
Size: 197 926 973 2083 901 262 1553
Fragments arranged by size:
2083 1553 973 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4686 493
Size: 4193 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6895
SphI G_CATG'C
Cuts at: 1132 4253 1132
Size: 3121 3774
SspI AAT'ATT
Cuts at: 5 52 2644 3628 4084 4819 5
Size: 47 2592 984 456 735 2081
Fragments arranged by size:
2592 2081 984 735 456 47
StuI AGG'CCT
Cuts at: 1517 3417 1517
Size: 1900 4995
StyI C'CwwG_G
Cuts at: 513 1065 2537 2892 3418 4334 513
Size: 552 1472 355 526 916 3074
Fragments arranged by size:
3074 1472 916 552 526 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4922 4939 5092 5277 5722 325
Size: 1255 3342 17 153 185 445 1498
Fragments arranged by size:
3342 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5141 6853 96
Size: 274 80 33 51 1443 3164 1712 138
Fragments arranged by size:
3164 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6855 6887 6855
Size: 32 6863
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6895
XbaI T'CTAG_A
Cuts at: 4231 4231
Size: 6895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 2698
Size: 6895
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6895
XmaI C'CCGG_G
Cuts at: 1870 4220 1870
Size: 2350 4545
XmnI GAAnn'nnTTC
Cuts at: 3255 3834 5024 3255
Size: 579 1190 5126
Fragments arranged by size:
5126 1190 579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4703 276
Size: 53 83 186 936 3169 2468
Fragments arranged by size:
3169 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI