Restriction map for
pFN21AB5140
(Circular) (Six-base) MAPSORT of: pFN21AB5140.seq Check: 848 from: 1 to: 5884
pFN21AB5140 5884 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2889 3227 1937
Size: 952 338 4594
Fragments arranged by size:
4594 952 338
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3947 5331 5599 1632
Size: 2315 1384 268 1917
Fragments arranged by size:
2315 1917 1384 268
AfeI AGC'GCT
Cuts at: 2072 2072
Size: 5884
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2159 2921 2998 1166
Size: 993 762 77 4052
Fragments arranged by size:
4052 993 762 77
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2432 1184
Size: 1248 4636
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 5852
AlwNI CAG_nnn'CTG
Cuts at: 2650 3026 3627 5199 2650
Size: 376 601 1572 3335
Fragments arranged by size:
3335 1572 601 376
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2402 2798 2831 3009 3209 1870
Size: 73 90 369 396 33 178 200 4545
Fragments arranged by size:
4545 396 369 200 178 90 73 33
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2055 2175 2231 2376 2556
Size: 325 217 627 268 120 56 145 180
Cuts at: 2556 2986 3205 4660 618
Size: 430 219 1455 1842
Fragments arranged by size:
1842 1455 627 430 325 268 219 217
180 145 120 56
BanII G_rGCy'C
Cuts at: 1391 2444 2879 3203 1391
Size: 1053 435 324 4072
Fragments arranged by size:
4072 1053 435 324
BbeI G_GCGC'C
Cuts at: 2059 2179 2235 2560 2059
Size: 120 56 325 5383
Fragments arranged by size:
5383 325 120 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2512 928
Size: 971 613 4300
Fragments arranged by size:
4300 971 613
BbvCI CC'TCA_GC
Cuts at: 2180 2687 2180
Size: 507 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2053 2406 4495
Size: 107 122 71 631 605 381 353 2089
Cuts at: 4495 136
Size: 1525
Fragments arranged by size:
2089 1525 631 605 381 353 122 107
71
BglII A'GATC_T
Cuts at: 2484 5879 2484
Size: 3395 2489
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2379 2402 2797 2991 3945 5101 1790
Size: 589 23 395 194 954 1156 2573
Fragments arranged by size:
2573 1156 954 589 395 194 23
BmgBI CAC'GTC
Cuts at: 1318 2919 1318
Size: 1601 4283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 4573 442
Size: 416 53 83 399 24 2198 958 1753
Fragments arranged by size:
2198 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2300 2672 2954 4544 1239
Size: 1052 9 372 282 1590 2579
Fragments arranged by size:
2579 1590 1052 372 282 9
Bpu10I CC'TnA_GC
Cuts at: 2180 2687 2180
Size: 507 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 2351 4547 882
Size: 617 846 6 2196 2219
Fragments arranged by size:
2219 2196 846 617 6
BsaAI yAC'GTr
Cuts at: 493 1204 2077 3675 493
Size: 711 873 1598 2702
Fragments arranged by size:
2702 1598 873 711
BsaBI GATnn'nnATC
Cuts at: 3045 5878 3045
Size: 2833 3051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2056 2176 2232 2297
Size: 53 83 186 936 523 120 56 65
Cuts at: 2297 2557 2760 2783 3691 4073 4708 275
Size: 260 203 23 908 382 635 1451
Fragments arranged by size:
1451 936 908 635 523 382 260 203
186 120 83 65 56 53 23
BsaWI w'CCGG_w
Cuts at: 4317 4715 4989 5136 5547 4317
Size: 398 274 147 411 4654
Fragments arranged by size:
4654 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 2645 2675 1178
Size: 30 165 30 1173 30 39 30 4387
Fragments arranged by size:
4387 1173 165 39 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2515 2584 3104 3107 1186
Size: 871 458 69 520 3 3963
Fragments arranged by size:
3963 871 520 458 69 3
BseYI C'CCAG_C
Cuts at: 3065 3125 5087 5583 3065
Size: 60 1962 496 3366
Fragments arranged by size:
3366 1962 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2657 3022 1163
Size: 573 387 534 365 4025
Fragments arranged by size:
4025 573 534 387 365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 2260 2716 3334 4095 5123
Size: 275 186 64 195 456 618 761 1028
Cuts at: 5123 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 618 456 275 195 186
64
BsiHKAI G_wGCw'C
Cuts at: 2277 2879 3010 3945 4030 5101 2277
Size: 602 131 935 85 1071 3060
Fragments arranged by size:
3060 1071 935 602 131 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
BsmBI CGTCTCn'nnnn_
Cuts at: 2703 2734 2703
Size: 31 5853
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2379 2402 2444 2797 2879 2991
Size: 399 487 102 23 42 353 82 112
Cuts at: 2991 3010 3203 3945 4030 5101 1391
Size: 19 193 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 399 353 193 112
102 85 82 42 23 19
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 3239 844
Size: 1331 1064 3489
Fragments arranged by size:
3489 1331 1064
BsrBI CCG'CTC
Cuts at: 1394 1823 2637 3771 1394
Size: 429 814 1134 3507
Fragments arranged by size:
3507 1134 814 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 3069 4528 4715 1700
Size: 110 281 978 1459 187 2869
Fragments arranged by size:
2869 1459 978 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 2503 1925
Size: 578 5306
BssSI C'ACGA_G
Cuts at: 2750 2972 3944 4956 2750
Size: 222 972 1012 3678
Fragments arranged by size:
3678 1012 972 222
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3026 794
Size: 2232 3652
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 3073 2161
Size: 912 4972
BstYI r'GATC_y
Cuts at: 1972 2484 3214 3581 3966 3983 5435 5521 5533
Size: 512 730 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 730 512 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2051 2059 2260 2445 2535 2850
Size: 552 939 47 8 201 185 90 315
Cuts at: 2850 513
Size: 3547
Fragments arranged by size:
3547 939 552 315 201 185 90 47
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 3010 3400 4194 4214 2177
Size: 833 390 794 20 3847
Fragments arranged by size:
3847 833 794 390 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DraIII CAC_nnn'GTG
Cuts at: 2292 2536 2605 2292
Size: 244 69 5571
Fragments arranged by size:
5571 244 69
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2089 2257
Size: 54 1425 50 275 18 232 27 168
Cuts at: 2257 2328 3331 4220 5849 8
Size: 71 1003 889 1629 43
Fragments arranged by size:
1629 1425 1003 889 275 232 168 71
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2062 2257 3331 1537
Size: 275 250 195 1074 4090
Fragments arranged by size:
4090 1074 275 250 195
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2345 2726 3814 1353
Size: 875 117 381 1088 3423
Fragments arranged by size:
3423 1088 875 381 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2877 2877
Size: 5884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2300 2672 2954 3947 4544 5331
Size: 393 659 9 372 282 993 597 787
Cuts at: 5331 5599 1239
Size: 268 1524
Fragments arranged by size:
1524 993 787 659 597 393 372 282
268 9
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2059 2074 2179 2235 2560 5031 1678
Size: 381 15 105 56 325 2471 2531
Fragments arranged by size:
2531 2471 381 325 105 56 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2576 2608 3158 3190 4525
Size: 32 135 32 1004 32 550 32 1335
Cuts at: 4525 4557 4599 4631 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1335 1004 550 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 3228 3474 669
Size: 988 281 370 920 246 3079
Fragments arranged by size:
3079 988 920 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 2055 2175 2231 2556 2055
Size: 120 56 325 5383
Fragments arranged by size:
5383 325 120 56
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4062
Fragments arranged by size:
4062 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2855 2921 2998 3842 4201 4360
Size: 648 993 696 66 77 844 359 159
Cuts at: 4360 518
Size: 2042
Fragments arranged by size:
2042 993 844 696 648 359 159 77
66
MspA1I CmG'CkG
Cuts at: 1606 2053 2061 2262 2671 2737 3300 3977 5125
Size: 447 8 201 409 66 563 677 1148
Cuts at: 5125 5370 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 563 447 409 245 201
66 8
NaeI GCC'GGC
Cuts at: 3071 3071
Size: 5884
NarI GG'CG_CC
Cuts at: 2056 2176 2232 2557 2056
Size: 120 56 325 5383
Fragments arranged by size:
5383 325 120 56
NcoI C'CATG_G
Cuts at: 513 1065 2004 2445 513
Size: 552 939 441 3952
Fragments arranged by size:
3952 939 552 441
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 3069 3069
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 2062 3331 2062
Size: 1269 4615
NspI r_CATG'y
Cuts at: 1132 1229 2476 2865 3242 4787 1132
Size: 97 1247 389 377 1545 2229
Fragments arranged by size:
2229 1545 1247 389 377 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5884
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2241 2650 1108
Size: 1133 409 4342
Fragments arranged by size:
4342 1133 409
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 3953 3985 5466 5498 2645
Size: 32 1276 32 1481 32 3031
Fragments arranged by size:
3031 1481 1276 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspXI vC'TCGA_Gb
Cuts at: 2831 2831
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2172 2430 3236 830
Size: 1342 258 806 3478
Fragments arranged by size:
3478 1342 806 258
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
SacI G_AGCT'C
Cuts at: 2879 2879
Size: 5884
SacII CC_GC'GG
Cuts at: 2054 2062 2263 2054
Size: 8 201 5675
Fragments arranged by size:
5675 201 8
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SapI GCTCTTCn'nnn_
Cuts at: 2228 2228
Size: 5884
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SexAI A'CCwGG_T
Cuts at: 2883 2883
Size: 5884
SfcI C'TryA_G
Cuts at: 826 1045 2168 2426 3232 4367 5048 5239 826
Size: 219 1123 258 806 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 806 681 258 219 191
SfoI GGC'GCC
Cuts at: 2057 2177 2233 2558 2057
Size: 120 56 325 5383
Fragments arranged by size:
5383 325 120 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 2035 3211 1872
Size: 163 1176 4545
Fragments arranged by size:
4545 1176 163
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2798 2831 3009 3988 4889 5151
Size: 197 926 855 33 178 979 901 262
Cuts at: 5151 820
Size: 1553
Fragments arranged by size:
1553 979 926 901 855 262 197 178
33
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SrfI GCCC'GGGC
Cuts at: 2035 2035
Size: 5884
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 1517
Size: 1095 4789
StyI C'CwwG_G
Cuts at: 513 1065 2004 2445 3177 3323 513
Size: 552 939 441 732 146 3074
Fragments arranged by size:
3074 939 732 552 441 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 2696 3911 3928 4081 4266 4711
Size: 1255 789 327 1215 17 153 185 445
Cuts at: 4711 325
Size: 1498
Fragments arranged by size:
1498 1255 1215 789 445 327 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2156 2188 2276 2308 5844 5876 2078
Size: 32 46 32 88 32 3536 32 2086
Fragments arranged by size:
3536 2086 88 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 2798 2831 3009 1943
Size: 855 33 178 4818
Fragments arranged by size:
4818 855 178 33
XmaI C'CCGG_G
Cuts at: 1870 2033 3209 1870
Size: 163 1176 4545
Fragments arranged by size:
4545 1176 163
XmnI GAAnn'nnTTC
Cuts at: 2456 4013 2456
Size: 1557 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SanDI SfiI SwaI XcmI