Restriction map for
pFN21AB5136
(Circular) (Six-base) MAPSORT of: pFN21AB5136.seq Check: 2284 from: 1 to: 5503
pFN21AB5136 5503 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2585 2585
Size: 5503
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3313 278
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2846 1937
Size: 909 4594
Acc65I G'GTAC_C
Cuts at: 2824 2824
Size: 5503
AclI AA'CG_TT
Cuts at: 1563 2650 3630 4003 1563
Size: 1087 980 373 3063
Fragments arranged by size:
3063 1087 980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 3566 4950 5218 1632
Size: 641 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 641 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5306
AflIII A'CryG_T
Cuts at: 1203 4402 5379 1203
Size: 3199 977 1327
Fragments arranged by size:
3199 1327 977
AgeI A'CCGG_T
Cuts at: 4334 4334
Size: 5503
AhdI GACnn_n'nnGTC
Cuts at: 4232 4232
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 2586 3246 4818 2586
Size: 660 1572 3271
Fragments arranged by size:
3271 1572 660
ApaLI G'TGCA_C
Cuts at: 3560 4716 3560
Size: 1156 4347
ApoI r'AATT_y
Cuts at: 1493 2812 3038 3190 5299 1493
Size: 1319 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4057 160
Size: 872 3025 1606
Fragments arranged by size:
3025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 1870
Size: 73 885 4545
Fragments arranged by size:
4545 885 73
BamHI G'GATC_C
Cuts at: 2833 3200 2833
Size: 367 5136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 4279 618
Size: 325 217 627 1037 1455 1842
Fragments arranged by size:
1842 1455 1037 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2822 1391
Size: 1431 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 928
Size: 971 429 4103
Fragments arranged by size:
4103 971 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3694 3728 1903
Size: 34 50 34 1673 34 3678
Fragments arranged by size:
3678 1673 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3395 4605 3395
Size: 1210 4293
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4114 136
Size: 107 122 71 631 605 2442 1525
Fragments arranged by size:
2442 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5498 5498
Size: 5503
BlpI GC'TnA_GC
Cuts at: 2920 2920
Size: 5503
Bme1580I G_kGCm'C
Cuts at: 1790 2322 3564 4720 1790
Size: 532 1242 1156 2573
Fragments arranged by size:
2573 1242 1156 532
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5503
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 2319 3234
Size: 416 53 83 399 24 883 19 915
Cuts at: 3234 4192 442
Size: 958 1753
Fragments arranged by size:
1753 958 915 883 416 399 83 53
24 19
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4163 1239
Size: 2924 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3628 4493 4791 3628
Size: 865 298 4340
Fragments arranged by size:
4340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2631 4166 882
Size: 617 1132 1535 2219
Fragments arranged by size:
2219 1535 1132 617
BsaAI yAC'GTr
Cuts at: 493 1204 2693 3294 493
Size: 711 1489 601 2702
Fragments arranged by size:
2702 1489 711 601
BsaBI GATnn'nnATC
Cuts at: 5497 5497
Size: 5503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3310 3692 4327 275
Size: 53 83 186 936 1777 382 635 1451
Fragments arranged by size:
1777 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3936 4334 4608 4755 5166 3936
Size: 398 274 147 411 4273
Fragments arranged by size:
4273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5278
Fragments arranged by size:
5278 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2583 1186
Size: 1397 4106
BseYI C'CCAG_C
Cuts at: 2188 4706 5202 2188
Size: 2518 496 2489
Fragments arranged by size:
2518 2489 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 1163
Size: 573 519 4411
Fragments arranged by size:
4411 573 519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3714 4742 1540
Size: 275 186 952 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2512 3564 3649 4720 2512
Size: 1052 85 1071 3295
Fragments arranged by size:
3295 1071 1052 85
BsmI GAATG_Cn'
Cuts at: 3014 3107 3014
Size: 93 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2322 2512 2822 3564 3649 4720 1391
Size: 399 532 190 310 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 399 310 190 85
BspEI T'CCGG_A
Cuts at: 5166 5166
Size: 5503
BspHI T'CATG_A
Cuts at: 3392 5122 3392
Size: 1730 3773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 2858 844
Size: 1741 273 3489
Fragments arranged by size:
3489 1741 273
BsrBI CCG'CTC
Cuts at: 1394 1823 3390 1394
Size: 429 1567 3507
Fragments arranged by size:
3507 1567 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3998 4172 65
Size: 1183 2750 174 1396
Fragments arranged by size:
2750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4147 4334 1700
Size: 110 2337 187 2869
Fragments arranged by size:
2869 2337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5503
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5503
BssSI C'ACGA_G
Cuts at: 3563 4575 3563
Size: 1012 4491
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2586 794
Size: 1792 3711
BstBI TT'CG_AA
Cuts at: 2119 4307 2119
Size: 2188 3315
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5503
BstYI r'GATC_y
Cuts at: 1972 2833 3200 3585 3602 5054 5140 5152 5498
Size: 861 367 385 17 1452 86 12 346
Cuts at: 5498 1972
Size: 1977
Fragments arranged by size:
1977 1452 861 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4951
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4764
Fragments arranged by size:
4764 683 56
BtsI GCAGTG_nn'
Cuts at: 2587 3019 3813 3833 2587
Size: 432 794 20 4257
Fragments arranged by size:
4257 794 432 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5468
DraI TTT'AAA
Cuts at: 2807 2807
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4510 809
Size: 507 3194 1802
Fragments arranged by size:
3194 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 2555 2950
Size: 54 1425 50 275 18 580 145 395
Cuts at: 2950 3839 5468 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 580 395 275 145 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2950 1537
Size: 275 1138 4090
Fragments arranged by size:
4090 1138 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3433 1353
Size: 2080 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2329 4090 4464 4610 387
Size: 1064 138 740 1761 374 146 1280
Fragments arranged by size:
1761 1280 1064 740 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 3566 4163 4950 5218 1239
Size: 393 641 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 641 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2185 2185
Size: 5503
EcoRI G'AATT_C
Cuts at: 2812 3190 2812
Size: 378 5125
EcoRV GAT'ATC
Cuts at: 2086 2086
Size: 5503
FspI TGC'GCA
Cuts at: 2458 4009 2458
Size: 1551 3952
HaeII r_GCGC'y
Cuts at: 1678 4650 1678
Size: 2972 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2286 2318 2628 2660 4144
Size: 32 135 32 714 32 310 32 1484
Cuts at: 4144 4176 4218 4250 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1484 714 310 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2626 2847 3093 669
Size: 988 281 688 221 246 3079
Fragments arranged by size:
3079 988 688 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5503
HpaI GTT'AAC
Cuts at: 2626 3093 2626
Size: 467 5036
KpnI G_GTAC'C
Cuts at: 2828 2828
Size: 5503
MfeI C'AATT_G
Cuts at: 2224 3102 2224
Size: 878 4625
MluI A'CGCG_T
Cuts at: 5379 5379
Size: 5503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4617 4801 1305
Size: 3312 184 2007
Fragments arranged by size:
3312 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 2557 10
Size: 54 1425 343 580 145 2956
Fragments arranged by size:
2956 1425 580 343 145 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3461 3820 3979 518
Size: 648 2295 359 159 2042
Fragments arranged by size:
2295 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2919 3596 4744 4989 1606
Size: 1313 677 1148 245 2120
Fragments arranged by size:
2120 1313 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4951
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5503
NotI GC'GGCC_GC
Cuts at: 2950 2950
Size: 5503
NspI r_CATG'y
Cuts at: 1132 1229 2861 4406 1132
Size: 97 1632 1545 2229
Fragments arranged by size:
2229 1632 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5503
PciI A'CATG_T
Cuts at: 4402 4402
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5503
PfoI T'CCnGG_A
Cuts at: 2367 2367
Size: 5503
PmeI GTTT'AAAC
Cuts at: 2807 2807
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 5085 5117 3572
Size: 32 1481 32 3958
Fragments arranged by size:
3958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2185
Size: 5503
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5503
PsiI TTA'TAA
Cuts at: 2753 3073 2753
Size: 320 5183
PstI C_TGCA'G
Cuts at: 830 2582 2855 830
Size: 1752 273 3478
Fragments arranged by size:
3478 1752 273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5503
SalI G'TCGA_C
Cuts at: 1936 2845 1936
Size: 909 4594
SanDI GG'GwC_CC
Cuts at: 2185 2185
Size: 5503
SbfI CC_TGCA'GG
Cuts at: 2855 2855
Size: 5503
ScaI AGT'ACT
Cuts at: 3751 5463 3751
Size: 1712 3791
SfcI C'TryA_G
Cuts at: 826 1045 2578 2851 3986 4667 4858 826
Size: 219 1533 273 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 273 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5503
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5503
SmaI CCC'GGG
Cuts at: 1872 2830 1872
Size: 958 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3607 4508 4770 820
Size: 197 926 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3294 493
Size: 2801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5503
SphI G_CATG'C
Cuts at: 1132 2861 1132
Size: 1729 3774
SspI AAT'ATT
Cuts at: 5 52 2074 3427 5
Size: 47 2022 1353 2081
Fragments arranged by size:
2081 2022 1353 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5503
StyI C'CwwG_G
Cuts at: 513 1065 2942 513
Size: 552 1877 3074
Fragments arranged by size:
3074 1877 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 3530 3547 3700 3885 4330 325
Size: 1255 709 1241 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1241 709 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3749 5461 96
Size: 274 80 33 51 1443 1772 1712 138
Fragments arranged by size:
1772 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5463 5495 5463
Size: 32 5471
Tth111I GACn'n_nGTC
Cuts at: 1316 2311 2642 1316
Size: 995 331 4177
Fragments arranged by size:
4177 995 331
XbaI T'CTAG_A
Cuts at: 2839 2839
Size: 5503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5503
XmaI C'CCGG_G
Cuts at: 1870 2828 1870
Size: 958 4545
XmnI GAAnn'nnTTC
Cuts at: 3632 3632
Size: 5503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3311 276
Size: 53 83 186 936 1777 2468
Fragments arranged by size:
2468 1777 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI