Restriction map for
pFN21AB5134
(Circular) (Six-base) MAPSORT of: pFN21AB5134.seq Check: 2218 from: 1 to: 7093
pFN21AB5134 7093 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2103 2131 2103
Size: 28 7065
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4903 278
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 4436 1937
Size: 883 1616 4594
Fragments arranged by size:
4594 1616 883
Acc65I G'GTAC_C
Cuts at: 4414 4414
Size: 7093
AclI AA'CG_TT
Cuts at: 1563 5220 5593 1563
Size: 3657 373 3063
Fragments arranged by size:
3657 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2538 3574 5156 6540 6808 1632
Size: 906 1036 1582 1384 268 1917
Fragments arranged by size:
1917 1582 1384 1036 906 268
AfeI AGC'GCT
Cuts at: 2153 2153
Size: 7093
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6896
AflIII A'CryG_T
Cuts at: 1203 2376 5992 6969 1203
Size: 1173 3616 977 1327
Fragments arranged by size:
3616 1327 1173 977
AgeI A'CCGG_T
Cuts at: 5924 5924
Size: 7093
AhdI GACnn_n'nnGTC
Cuts at: 5822 5822
Size: 7093
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7093
AlwNI CAG_nnn'CTG
Cuts at: 3197 4836 6408 3197
Size: 1639 1572 3882
Fragments arranged by size:
3882 1639 1572
ApaLI G'TGCA_C
Cuts at: 3059 5150 6306 3059
Size: 2091 1156 3846
Fragments arranged by size:
3846 2091 1156
ApoI r'AATT_y
Cuts at: 1493 2435 3622 4402 4628 4780 6889 1493
Size: 942 1187 780 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 942 780 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2460 3303 5647 160
Size: 872 1428 843 2344 1606
Fragments arranged by size:
2344 1606 1428 872 843
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
AvaI C'yCGr_G
Cuts at: 1870 1943 4418 1870
Size: 73 2475 4545
Fragments arranged by size:
4545 2475 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4085 4118 4085
Size: 33 7060
BamHI G'GATC_C
Cuts at: 4423 4790 4423
Size: 367 6726
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2115 3200 3857 4414
Size: 325 217 627 304 24 1085 657 557
Cuts at: 4414 5869 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1085 657 627 557 325 304
217 24
BanII G_rGCy'C
Cuts at: 1391 2371 3144 4128 4253 4274 4412 1391
Size: 980 773 984 125 21 138 4072
Fragments arranged by size:
4072 984 980 773 138 125 21
BbeI G_GCGC'C
Cuts at: 3861 3861
Size: 7093
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4023 928
Size: 971 2124 3998
Fragments arranged by size:
3998 2124 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5284 5318 1903
Size: 34 3347 34 3678
Fragments arranged by size:
3678 3347 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2615 4985 6195 2615
Size: 2370 1210 3513
Fragments arranged by size:
3513 2370 1210
BclI T'GATC_A
Cuts at: 1548 3502 3723 1548
Size: 1954 221 4918
Fragments arranged by size:
4918 1954 221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5704 136
Size: 107 122 71 631 605 4032 1525
Fragments arranged by size:
4032 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7088 7088
Size: 7093
BlpI GC'TnA_GC
Cuts at: 4510 4510
Size: 7093
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2118 3063 3805 5154 6310 1790
Size: 304 24 945 742 1349 1156 2573
Fragments arranged by size:
2573 1349 1156 945 742 304 24
BmgBI CAC'GTC
Cuts at: 1318 3793 1318
Size: 2475 4618
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2365 3416 4336
Size: 416 53 83 399 24 948 1051 920
Cuts at: 4336 4824 5782 442
Size: 488 958 1753
Fragments arranged by size:
1753 1051 958 948 920 488 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 4031 5753 1239
Size: 1202 1590 1722 2579
Fragments arranged by size:
2579 1722 1590 1202
Bpu10I CC'TnA_GC
Cuts at: 2388 3848 4268 2388
Size: 1460 420 5213
Fragments arranged by size:
5213 1460 420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5218 6083 6381 5218
Size: 865 298 5930
Fragments arranged by size:
5930 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 5756 882
Size: 617 984 3273 2219
Fragments arranged by size:
3273 2219 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 2704 4884 493
Size: 711 1500 2180 2702
Fragments arranged by size:
2702 2180 1500 711
BsaBI GATnn'nnATC
Cuts at: 3869 7087 3869
Size: 3218 3875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3858 4900 5282 5917
Size: 53 83 186 936 2325 1042 382 635
Cuts at: 5917 275
Size: 1451
Fragments arranged by size:
2325 1451 1042 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2473 5526 5924 6198 6345 6756 2473
Size: 3053 398 274 147 411 2810
Fragments arranged by size:
3053 2810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 4110 4140 4192
Size: 30 165 30 1011 30 1666 30 52
Cuts at: 4192 4222 1178
Size: 30 4049
Fragments arranged by size:
4049 1666 1011 165 52 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2708 4197 1186
Size: 1024 498 1489 4082
Fragments arranged by size:
4082 1489 1024 498
BseYI C'CCAG_C
Cuts at: 2166 6296 6792 2166
Size: 4130 496 2467
Fragments arranged by size:
4130 2467 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2468 3567 1163
Size: 573 732 1099 4689
Fragments arranged by size:
4689 1099 732 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4543 5304 6332 1540
Size: 275 186 1270 1272 761 1028 2301
Fragments arranged by size:
2301 1272 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3063 3854 4274 5154 5239 6310 3063
Size: 791 420 880 85 1071 3846
Fragments arranged by size:
3846 1071 880 791 420 85
BsmI GAATG_Cn'
Cuts at: 4604 4697 4604
Size: 93 7000
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2118 2371 3063 3144 3805 3854
Size: 399 304 24 253 692 81 661 49
Cuts at: 3854 4128 4253 4274 4412 5154 5239 6310 1391
Size: 274 125 21 138 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 661 399 304 274
253 138 125 85 81 49 24 21
BspEI T'CCGG_A
Cuts at: 2473 6756 2473
Size: 4283 2810
BspHI T'CATG_A
Cuts at: 4982 6712 4982
Size: 1730 5363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2131 4448 844
Size: 1259 28 2317 3489
Fragments arranged by size:
3489 2317 1259 28
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 4980 1394
Size: 429 260 2897 3507
Fragments arranged by size:
3507 2897 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 3091 5588 5762 65
Size: 1183 1843 2497 174 1396
Fragments arranged by size:
2497 1843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3842 5737 5924 1700
Size: 110 2032 1895 187 2869
Fragments arranged by size:
2869 2032 1895 187 110
BsrGI T'GTAC_A
Cuts at: 96 2598 96
Size: 2502 4591
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7093
BssSI C'ACGA_G
Cuts at: 2486 4171 5153 6165 2486
Size: 1685 982 1012 3414
Fragments arranged by size:
3414 1685 1012 982
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2055 794
Size: 1261 5832
BstBI TT'CG_AA
Cuts at: 5897 5897
Size: 7093
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7093
BstXI CCAn_nnnn'nTGG
Cuts at: 2350 2725 4147 2350
Size: 375 1422 5296
Fragments arranged by size:
5296 1422 375
BstYI r'GATC_y
Cuts at: 1972 2884 4423 4790 5175 5192 6644 6730 6742
Size: 912 1539 367 385 17 1452 86 12
Cuts at: 6742 7088 1972
Size: 346 1977
Fragments arranged by size:
1977 1539 1452 912 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2821 2821
Size: 7093
BtgI C'CryG_G
Cuts at: 513 1065 2110 3204 4200 513
Size: 552 1045 1094 996 3406
Fragments arranged by size:
3406 1094 1045 996 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3744 487
Size: 683 56 2518 3836
Fragments arranged by size:
3836 2518 683 56
BtsI GCAGTG_nn'
Cuts at: 3803 4609 5403 5423 3803
Size: 806 794 20 5473
Fragments arranged by size:
5473 806 794 20
ClaI AT'CG_AT
Cuts at: 1554 3267 1554
Size: 1713 5380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7058
DraI TTT'AAA
Cuts at: 4397 4397
Size: 7093
DraIII CAC_nnn'GTG
Cuts at: 2707 2707
Size: 7093
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6100 809
Size: 507 4784 1802
Fragments arranged by size:
4784 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2414 3426 3567
Size: 54 1425 50 275 18 584 1012 141
Cuts at: 3567 4130 4257 4284 4540 5429 7058 8
Size: 563 127 27 256 889 1629 43
Fragments arranged by size:
1629 1425 1012 889 584 563 275 256
141 127 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 4540 1537
Size: 275 2728 4090
Fragments arranged by size:
4090 2728 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2863 5023 1353
Size: 1002 508 2160 3423
Fragments arranged by size:
3423 2160 1002 508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2757 5680 6054 6200 387
Size: 1064 138 1168 2923 374 146 1280
Fragments arranged by size:
2923 1280 1168 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4272 4272
Size: 7093
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2538 3574 4031 5156 5753 6540
Size: 393 809 97 1036 457 1125 597 787
Cuts at: 6540 6808 1239
Size: 268 1524
Fragments arranged by size:
1524 1125 1036 809 787 597 457 393
268 97
EcoNI CCTnn'n_nnAGG
Cuts at: 3480 3480
Size: 7093
EcoO109I rG'GnC_Cy
Cuts at: 3436 4263 4331 3436
Size: 827 68 6198
Fragments arranged by size:
6198 827 68
EcoRI G'AATT_C
Cuts at: 2435 4402 4780 2435
Size: 1967 378 4748
Fragments arranged by size:
4748 1967 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 7093
FspI TGC'GCA
Cuts at: 5599 5599
Size: 7093
HaeII r_GCGC'y
Cuts at: 1678 2155 2589 3861 4139 6240 1678
Size: 477 434 1272 278 2101 2531
Fragments arranged by size:
2531 2101 1272 477 434 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2306 2338 2370
Size: 32 135 32 662 32 40 32 32
Cuts at: 2370 2414 2446 4110 4142 5734 5766 5808 5840
Size: 44 32 1664 32 1592 32 42 32
Cuts at: 5840 1373
Size: 2626
Fragments arranged by size:
2626 1664 1592 662 135 44 42 40
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 669 1657 1938 3448 4437 4683 669
Size: 988 281 1510 989 246 3079
Fragments arranged by size:
3079 1510 989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7093
HpaI GTT'AAC
Cuts at: 4683 4683
Size: 7093
KasI G'GCGC_C
Cuts at: 3857 3857
Size: 7093
KpnI G_GTAC'C
Cuts at: 4418 4418
Size: 7093
MfeI C'AATT_G
Cuts at: 3531 4692 3531
Size: 1161 5932
MluI A'CGCG_T
Cuts at: 6969 6969
Size: 7093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 2412 3546 6207 6391 1305
Size: 1026 81 1134 2661 184 2007
Fragments arranged by size:
2661 2007 1134 1026 184 81
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3428 10
Size: 54 1425 343 1596 3675
Fragments arranged by size:
3675 1596 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 2306 2381 5051 5410 5569 518
Size: 648 898 242 75 2670 359 159 2042
Fragments arranged by size:
2670 2042 898 648 359 242 159 75
MspA1I CmG'CkG
Cuts at: 1606 3194 4509 5186 6334 6579 1606
Size: 1588 1315 677 1148 245 2120
Fragments arranged by size:
2120 1588 1315 1148 677 245
NaeI GCC'GGC
Cuts at: 3844 3844
Size: 7093
NarI GG'CG_CC
Cuts at: 3858 3858
Size: 7093
NcoI C'CATG_G
Cuts at: 513 1065 2110 513
Size: 552 1045 5496
Fragments arranged by size:
5496 1045 552
NdeI CA'TA_TG
Cuts at: 387 2736 387
Size: 2349 4744
NgoMIV G'CCGG_C
Cuts at: 3842 3842
Size: 7093
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7093
NotI GC'GGCC_GC
Cuts at: 4540 4540
Size: 7093
NspI r_CATG'y
Cuts at: 1132 1229 2053 3224 4114 4451 5996 1132
Size: 97 824 1171 890 337 1545 2229
Fragments arranged by size:
2229 1545 1171 890 824 337 97
PasI CC'CwG_GG
Cuts at: 1348 2852 1348
Size: 1504 5589
PciI A'CATG_T
Cuts at: 5992 5992
Size: 7093
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2894 3314 1108
Size: 1786 420 4887
Fragments arranged by size:
4887 1786 420
PfoI T'CCnGG_A
Cuts at: 2451 2451
Size: 7093
PmeI GTTT'AAAC
Cuts at: 4397 4397
Size: 7093
PmlI CAC'GTG
Cuts at: 2704 2704
Size: 7093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5162 5194 6675 6707 5162
Size: 32 1481 32 5548
Fragments arranged by size:
5548 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3436 4263 4331 3436
Size: 827 68 6198
Fragments arranged by size:
6198 827 68
PshAI GACnn'nnGTC
Cuts at: 1613 2783 1613
Size: 1170 5923
PsiI TTA'TAA
Cuts at: 4663 4663
Size: 7093
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 7061
PstI C_TGCA'G
Cuts at: 830 4445 830
Size: 3615 3478
PvuI CG_AT'CG
Cuts at: 2001 3271 2001
Size: 1270 5823
PvuII CAG'CTG
Cuts at: 3194 3194
Size: 7093
SacI G_AGCT'C
Cuts at: 4274 4274
Size: 7093
SalI G'TCGA_C
Cuts at: 1936 4435 1936
Size: 2499 4594
SanDI GG'GwC_CC
Cuts at: 4263 4263
Size: 7093
SbfI CC_TGCA'GG
Cuts at: 4445 4445
Size: 7093
ScaI AGT'ACT
Cuts at: 5341 7053 5341
Size: 1712 5381
SfcI C'TryA_G
Cuts at: 826 1045 2147 3006 3031 4319 4441 5576 6257
Size: 219 1102 859 25 1288 122 1135 681
Cuts at: 6257 6448 826
Size: 191 1471
Fragments arranged by size:
1471 1288 1135 1102 859 681 219 191
122 25
SfoI GGC'GCC
Cuts at: 3859 3859
Size: 7093
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7093
SmaI CCC'GGG
Cuts at: 1872 4420 1872
Size: 2548 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5197 6098 6360 820
Size: 197 926 3254 901 262 1553
Fragments arranged by size:
3254 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4884 493
Size: 4391 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7093
SphI G_CATG'C
Cuts at: 1132 2053 3224 4451 1132
Size: 921 1171 1227 3774
Fragments arranged by size:
3774 1227 1171 921
SspI AAT'ATT
Cuts at: 5 52 2923 5017 5
Size: 47 2871 2094 2081
Fragments arranged by size:
2871 2094 2081 47
StuI AGG'CCT
Cuts at: 1517 3827 1517
Size: 2310 4783
StyI C'CwwG_G
Cuts at: 513 1065 2110 2688 2718 4532 513
Size: 552 1045 578 30 1814 3074
Fragments arranged by size:
3074 1814 1045 578 552 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 2795 5120 5137 5290 5475 5920
Size: 1255 1137 78 2325 17 153 185 445
Cuts at: 5920 325
Size: 1498
Fragments arranged by size:
2325 1498 1255 1137 445 185 153 78
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 2373 2598
Size: 274 80 33 51 1443 222 174 225
Cuts at: 2598 4107 5339 7051 96
Size: 1509 1232 1712 138
Fragments arranged by size:
1712 1509 1443 1232 274 225 222 174
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3289 3321 4192 4224 7053 7085 3289
Size: 32 871 32 2829 32 3297
Fragments arranged by size:
3297 2829 871 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7093
XbaI T'CTAG_A
Cuts at: 4429 4429
Size: 7093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2416 3421 4055 2347
Size: 69 1005 634 5385
Fragments arranged by size:
5385 1005 634 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7093
XmaI C'CCGG_G
Cuts at: 1870 4418 1870
Size: 2548 4545
XmnI GAAnn'nnTTC
Cuts at: 2180 3609 5222 2180
Size: 1429 1613 4051
Fragments arranged by size:
4051 1613 1429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4901 276
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI Bsu36I EcoRV FseI FspAI NruI NsiI PacI
PspOMI PspXI RsrII SacII SapI SexAI SfiI SrfI
SwaI