Restriction map for
pFN21AB5126
(Circular) (Six-base) MAPSORT of: pFN21AB5126.seq Check: 8210 from: 1 to: 5731
pFN21AB5126 5731 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2464 2464
Size: 5731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2390 3074 1937
Size: 453 684 4594
Fragments arranged by size:
4594 684 453
Acc65I G'GTAC_C
Cuts at: 2109 3052 2109
Size: 943 4788
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 2112 4562 2112
Size: 2450 3281
AhdI GACnn_n'nnGTC
Cuts at: 2386 4460 2386
Size: 2074 3657
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2309 3008 3474 5046 2309
Size: 699 466 1572 2994
Fragments arranged by size:
2994 1572 699 466
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 2577 3040 3266 3418 5527 1493
Size: 1084 463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1084 463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2135 3056 1870
Size: 73 103 89 921 4545
Fragments arranged by size:
4545 921 103 89 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 3052 4507 618
Size: 325 217 627 322 943 1455 1842
Fragments arranged by size:
1842 1455 943 627 325 322 217
BanII G_rGCy'C
Cuts at: 1391 2411 2500 2756 3050 1391
Size: 1020 89 256 294 4072
Fragments arranged by size:
4072 1020 294 256 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2838 928
Size: 971 939 3821
Fragments arranged by size:
3821 971 939
BbvCI CC'TCA_GC
Cuts at: 2670 2670
Size: 5731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2288 2322 3922 3956 1903
Size: 34 50 34 267 34 1600 34 3678
Fragments arranged by size:
3678 1600 267 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2584 3623 4833 2584
Size: 1039 1210 3482
Fragments arranged by size:
3482 1210 1039
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 2469 2755 3148 2469
Size: 286 393 5052
Fragments arranged by size:
5052 393 286
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2988 3792 4948 1790
Size: 247 951 804 1156 2573
Fragments arranged by size:
2573 1156 951 804 247
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2764 2790 3462
Size: 416 53 83 399 24 1347 26 672
Cuts at: 3462 4420 442
Size: 958 1753
Fragments arranged by size:
1753 1347 958 672 416 399 83 53
26 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2128 2268 4391 1239
Size: 889 140 2123 2579
Fragments arranged by size:
2579 2123 889 140
Bpu10I CC'TnA_GC
Cuts at: 2508 2670 2750 2508
Size: 162 80 5489
Fragments arranged by size:
5489 162 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2425 2842 3856 4721 5019 2425
Size: 417 1014 865 298 3137
Fragments arranged by size:
3137 1014 865 417 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2112 4164 4562 4836 4983 5394 2112
Size: 2052 398 274 147 411 2449
Fragments arranged by size:
2449 2052 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2523 1186
Size: 937 400 4394
Fragments arranged by size:
4394 937 400
BseYI C'CCAG_C
Cuts at: 2163 2955 3001 4934 5430 2163
Size: 792 46 1933 496 2464
Fragments arranged by size:
2464 1933 792 496 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2411 2500 2756 3792 3877 4948 2411
Size: 89 256 1036 85 1071 3194
Fragments arranged by size:
3194 1071 1036 256 89 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2411 2500 2756 2988 3050 3792
Size: 399 247 374 89 256 232 62 742
Cuts at: 3792 3877 4948 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 256 247 232
89 85 62
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2464 3022 3086 844
Size: 1620 558 64 3489
Fragments arranged by size:
3489 1620 558 64
BsrBI CCG'CTC
Cuts at: 1394 1823 2926 3618 1394
Size: 429 1103 692 3507
Fragments arranged by size:
3507 1103 692 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2112 2168 4375 4562 1700
Size: 110 302 56 2207 187 2869
Fragments arranged by size:
2869 2207 302 187 110 56
BsrGI T'GTAC_A
Cuts at: 96 2761 96
Size: 2665 3066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2361 794
Size: 1567 4164
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726
Size: 1089 367 385 17 1452 86 12 346
Cuts at: 5726 1972
Size: 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 2635 3247 4041 4061 2635
Size: 612 794 20 4305
Fragments arranged by size:
4305 794 612 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 2804 2983 2804
Size: 179 5552
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2966 4738 809
Size: 507 1650 1772 1802
Fragments arranged by size:
1802 1772 1650 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696
Size: 54 1425 50 275 18 1348 889 1629
Cuts at: 5696 8
Size: 43
Fragments arranged by size:
1629 1425 1348 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 1353
Size: 2308 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2215 4318 4692 4838 387
Size: 1064 138 626 2103 374 146 1280
Fragments arranged by size:
2103 1280 1064 626 374 146 138
EcoICRI GAG'CTC
Cuts at: 2409 2498 2754 2409
Size: 89 256 5386
Fragments arranged by size:
5386 256 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2268 3794 4391 5178 5446 1239
Size: 393 496 140 1526 597 787 268 1524
Fragments arranged by size:
1526 1524 787 597 496 393 268 140
EcoO109I rG'GnC_Cy
Cuts at: 2139 2191 2461 2139
Size: 52 270 5409
Fragments arranged by size:
5409 270 52
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2129 2161 4878 1678
Size: 451 32 2717 2531
Fragments arranged by size:
2717 2531 451 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 4372 4404 4446
Size: 32 135 32 800 32 1968 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 1968 800 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 2113 3056 2113
Size: 943 4788
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 4845 5029 1305
Size: 771 2769 184 2007
Fragments arranged by size:
2769 2007 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2598 2759 3147 3824 4972 5217 1606
Size: 992 161 388 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 388 245 161
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 5731
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 3089 4634 1132
Size: 97 1860 1545 2229
Fragments arranged by size:
2229 1860 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3008 1108
Size: 1900 3831
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2461 2191
Size: 270 5461
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2630 3083 830
Size: 1800 453 3478
Fragments arranged by size:
3478 1800 453
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2759 2759
Size: 5731
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 5731
SacI G_AGCT'C
Cuts at: 2411 2500 2756 2411
Size: 89 256 5386
Fragments arranged by size:
5386 256 89
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2391 2626 3079 4214 4895 5086 826
Size: 219 1346 235 453 1135 681 191 1471
Fragments arranged by size:
1471 1346 1135 681 453 235 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 2048 3058 1872
Size: 176 1010 4545
Fragments arranged by size:
4545 1010 176
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2404 2857 3835 4736 4998 820
Size: 197 926 461 453 978 901 262 1553
Fragments arranged by size:
1553 978 926 901 461 453 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3074
Fragments arranged by size:
3074 2105 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3758 3775 3928 4113 4558 325
Size: 1255 2178 17 153 185 445 1498
Fragments arranged by size:
2178 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2735 2761 3977
Size: 274 80 33 51 1443 758 26 1216
Cuts at: 3977 5689 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1216 758 274 138 80 51
33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 2917 1316
Size: 1601 4130
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2243 2777 2243
Size: 534 5197
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 2046 3056 1870
Size: 176 1010 4545
Fragments arranged by size:
4545 1010 176
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI