Restriction map for
pFN21AB5121
(Circular) (Six-base) MAPSORT of: pFN21AB5121.seq Check: 7754 from: 1 to: 6127
pFN21AB5121 6127 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2187 3087 3470 1937
Size: 250 900 383 4594
Fragments arranged by size:
4594 900 383 250
Acc65I G'GTAC_C
Cuts at: 2356 3271 3448 2356
Size: 915 177 5035
Fragments arranged by size:
5035 915 177
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4190 5574 5842 1632
Size: 2558 1384 268 1917
Fragments arranged by size:
2558 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2103 3424 5026 6003 1203
Size: 900 1321 1602 977 1327
Fragments arranged by size:
1602 1327 1321 977 900
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 3358 4856 3358
Size: 1498 4629
AleI CACnn'nnGTG
Cuts at: 1166 2849 3161 1166
Size: 1683 312 4132
Fragments arranged by size:
4132 1683 312
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 2606 3870 5442 2606
Size: 1264 1572 3291
Fragments arranged by size:
3291 1572 1264
ApaI G_GGCC'C
Cuts at: 2181 2181
Size: 6127
ApaLI G'TGCA_C
Cuts at: 2076 4184 5340 2076
Size: 2108 1156 2863
Fragments arranged by size:
2863 2108 1156
ApoI r'AATT_y
Cuts at: 1493 2573 3343 3436 3662 3814 5923 1493
Size: 1080 770 93 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 770 226 152 93
AscI GG'CGCG_CC
Cuts at: 3250 3250
Size: 6127
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 1870
Size: 73 1509 4545
Fragments arranged by size:
4545 1509 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 3271 3448 4903 618
Size: 325 217 627 569 915 177 1455 1842
Fragments arranged by size:
1842 1455 915 627 569 325 217 177
BanII G_rGCy'C
Cuts at: 1391 2181 3446 1391
Size: 790 1265 4072
Fragments arranged by size:
4072 1265 790
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5156
BbvCI CC'TCA_GC
Cuts at: 3110 3110
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2703 4019 5229 2703
Size: 1316 1210 3601
Fragments arranged by size:
3601 1316 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BfrBI ATG'CAT
Cuts at: 2816 2816
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2080 2181 4188 5344 1790
Size: 290 101 2007 1156 2573
Fragments arranged by size:
2573 2007 1156 290 101
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3030 1056
Size: 1974 4153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 2990 3086 4787 1239
Size: 1316 435 96 1701 2579
Fragments arranged by size:
2579 1701 1316 435 96
Bpu10I CC'TnA_GC
Cuts at: 3110 3227 3110
Size: 117 6010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2233 2882 4790 882
Size: 617 734 649 1908 2219
Fragments arranged by size:
2219 1908 734 649 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 3918 493
Size: 711 909 1805 2702
Fragments arranged by size:
2702 1805 909 711
BsaBI GATnn'nnATC
Cuts at: 2201 2928 6121 2201
Size: 727 3193 2207
Fragments arranged by size:
3193 2207 727
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3090 3934 4316 4951
Size: 53 83 186 936 1557 844 382 635
Cuts at: 4951 275
Size: 1451
Fragments arranged by size:
1557 1451 936 844 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3126 4560 4958 5232 5379 5790 3126
Size: 1434 398 274 147 411 3463
Fragments arranged by size:
3463 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 1178
Size: 30 165 30 810 30 5062
Fragments arranged by size:
5062 810 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2604 3098 1186
Size: 1418 494 4215
Fragments arranged by size:
4215 1418 494
BseYI C'CCAG_C
Cuts at: 2096 3275 3306 5330 5826 2096
Size: 1179 31 2024 496 2397
Fragments arranged by size:
2397 2024 1179 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 1163
Size: 573 776 4778
Fragments arranged by size:
4778 776 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2165 3577 4338 5366 1540
Size: 275 186 164 1412 761 1028 2301
Fragments arranged by size:
2301 1412 1028 761 275 186 164
BsiHKAI G_wGCw'C
Cuts at: 2080 3156 4188 4273 5344 2080
Size: 1076 1032 85 1071 2863
Fragments arranged by size:
2863 1076 1071 1032 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
BsmBI CGTCTCn'nnnn_
Cuts at: 3128 3128
Size: 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2181 3156 3446 4188 4273 5344
Size: 399 290 101 975 290 742 85 1071
Cuts at: 5344 1391
Size: 2174
Fragments arranged by size:
2174 1071 975 742 399 290 290 101
85
BspEI T'CCGG_A
Cuts at: 3126 5790 3126
Size: 2664 3463
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3261 4771 4958 1700
Size: 110 1451 1510 187 2869
Fragments arranged by size:
2869 1510 1451 187 110
BsrGI T'GTAC_A
Cuts at: 96 3421 96
Size: 3325 2802
BssHII G'CGCG_C
Cuts at: 1925 3250 1925
Size: 1325 4802
BssSI C'ACGA_G
Cuts at: 3157 4187 5199 3157
Size: 1030 1012 4085
Fragments arranged by size:
4085 1030 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 2500 4931 2500
Size: 2431 3696
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 2494 2901 3321 3457 3824 4209 4226 5678
Size: 522 407 420 136 367 385 17 1452
Cuts at: 5678 5764 5776 6122 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 522 420 407 385 367 346
136 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2882 2882
Size: 6127
BtgI C'CryG_G
Cuts at: 513 1065 2064 3094 513
Size: 552 999 1030 3546
Fragments arranged by size:
3546 1030 999 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 2205 1554
Size: 651 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2162 3574 4463
Size: 54 1425 50 275 18 332 1412 889
Cuts at: 4463 6092 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1412 889 332 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2162 3574 1537
Size: 275 350 1412 4090
Fragments arranged by size:
4090 1412 350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4057 1353
Size: 2704 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2555 2990 3086 4190 4787 5574 5842
Size: 393 923 435 96 1104 597 787 268
Cuts at: 5842 1239
Size: 1524
Fragments arranged by size:
1524 1104 923 787 597 435 393 268
96
EcoO109I rG'GnC_Cy
Cuts at: 2177 2178 3008 2177
Size: 1 830 5296
Fragments arranged by size:
5296 830 1
EcoRI G'AATT_C
Cuts at: 3343 3436 3814 3343
Size: 93 378 5656
Fragments arranged by size:
5656 378 93
EcoRV GAT'ATC
Cuts at: 2507 2507
Size: 6127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2313 2313
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 3204 5274 1678
Size: 1526 2070 2531
Fragments arranged by size:
2531 2070 1526
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2372 2404 2451
Size: 32 135 32 641 32 127 32 47
Cuts at: 2451 2483 3344 3376 4768 4800 4842 4874 1373
Size: 32 861 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 861 641 135 127 47 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2188 2647 3088 3471 3717 669
Size: 988 281 250 459 441 383 246 3079
Fragments arranged by size:
3079 988 459 441 383 281 250 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 2360 3275 3452 2360
Size: 915 177 5035
Fragments arranged by size:
5035 915 177
MfeI C'AATT_G
Cuts at: 2640 3726 2640
Size: 1086 5041
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2439 2849 3161 4085 4444 4603
Size: 648 1056 217 410 312 924 359 159
Cuts at: 4603 518
Size: 2042
Fragments arranged by size:
2042 1056 924 648 410 359 312 217
159
MspA1I CmG'CkG
Cuts at: 1606 2480 3543 4220 5368 5613 1606
Size: 874 1063 677 1148 245 2120
Fragments arranged by size:
2120 1148 1063 874 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2064 513
Size: 552 999 4576
Fragments arranged by size:
4576 999 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 3026 1052
Size: 1974 4153
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2818 2818
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2107 2487 2761 3485 5030 1132
Size: 97 878 380 274 724 1545 2229
Fragments arranged by size:
2229 1545 878 724 380 274 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 2103 5026 2103
Size: 2923 3204
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 2177 2177
Size: 6127
PstI C_TGCA'G
Cuts at: 830 2824 3479 830
Size: 1994 655 3478
Fragments arranged by size:
3478 1994 655
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2480 2480
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 2186 3086 3469 1936
Size: 250 900 383 4594
Fragments arranged by size:
4594 900 383 250
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2820 3475 4610 5291 5482 826
Size: 219 1775 655 1135 681 191 1471
Fragments arranged by size:
1775 1471 1135 681 655 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4231 5132 5394 820
Size: 197 926 2288 901 262 1553
Fragments arranged by size:
2288 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 3242 1517
Size: 1725 4402
StyI C'CwwG_G
Cuts at: 513 1065 2064 3174 3566 513
Size: 552 999 1110 392 3074
Fragments arranged by size:
3074 1110 999 552 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 2891 4154 4171 4324 4509 4954
Size: 1255 1199 112 1263 17 153 185 445
Cuts at: 4954 325
Size: 1498
Fragments arranged by size:
1498 1263 1255 1199 445 185 153 112
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2014 3186 3421
Size: 274 80 33 51 1443 37 1172 235
Cuts at: 3421 4373 6085 96
Size: 952 1712 138
Fragments arranged by size:
1712 1443 1172 952 274 235 138 80
51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6087 6119 6087
Size: 32 6095
Tth111I GACn'n_nGTC
Cuts at: 1316 2225 1316
Size: 909 5218
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2221
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbeI BplI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI