Restriction map for
pFN21AB5115
(Circular) (Six-base) MAPSORT of: pFN21AB5115.seq Check: 5624 from: 1 to: 7996
pFN21AB5115 7996 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4159 4159
Size: 7996
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5806 278
Size: 53 83 186 936 4270 2468
Fragments arranged by size:
4270 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2745 2829 5339 1937
Size: 808 84 2510 4594
Fragments arranged by size:
4594 2510 808 84
Acc65I G'GTAC_C
Cuts at: 5317 5317
Size: 7996
AclI AA'CG_TT
Cuts at: 1563 6123 6496 1563
Size: 4560 373 3063
Fragments arranged by size:
4560 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2535 4670 4917 6059 7443 7711 1632
Size: 903 2135 247 1142 1384 268 1917
Fragments arranged by size:
2135 1917 1384 1142 903 268 247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7799
AflIII A'CryG_T
Cuts at: 1203 2091 2834 3139 3151 5070 6895 7872 1203
Size: 888 743 305 12 1919 1825 977 1327
Fragments arranged by size:
1919 1825 1327 977 888 743 305 12
AgeI A'CCGG_T
Cuts at: 6827 6827
Size: 7996
AhdI GACnn_n'nnGTC
Cuts at: 5071 6725 5071
Size: 1654 6342
AleI CACnn'nnGTG
Cuts at: 1166 2774 1166
Size: 1608 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7996
AlwNI CAG_nnn'CTG
Cuts at: 2190 2513 5739 7311 2190
Size: 323 3226 1572 2875
Fragments arranged by size:
3226 2875 1572 323
ApaI G_GGCC'C
Cuts at: 2648 3879 2648
Size: 1231 6765
ApaLI G'TGCA_C
Cuts at: 6053 7209 6053
Size: 1156 6840
ApoI r'AATT_y
Cuts at: 1493 2558 3539 4615 4891 5305 5531 5683 7792
Size: 1065 981 1076 276 414 226 152 2109
Cuts at: 7792 1493
Size: 1697
Fragments arranged by size:
2109 1697 1076 1065 981 414 276 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6550 160
Size: 872 5518 1606
Fragments arranged by size:
5518 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7996
AvaI C'yCGr_G
Cuts at: 1870 1943 5321 1870
Size: 73 3378 4545
Fragments arranged by size:
4545 3378 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2737 2770 2737
Size: 33 7963
BamHI G'GATC_C
Cuts at: 5326 5693 5326
Size: 367 7629
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3926 4034 5317 6772 618
Size: 325 217 627 2139 108 1283 1455 1842
Fragments arranged by size:
2139 1842 1455 1283 627 325 217 108
BanII G_rGCy'C
Cuts at: 1391 2465 2648 3879 5110 5315 1391
Size: 1074 183 1231 1231 205 4072
Fragments arranged by size:
4072 1231 1231 1074 205 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 2474 2957 928
Size: 971 123 452 483 5967
Fragments arranged by size:
5967 971 483 452 123
BbvCI CC'TCA_GC
Cuts at: 2136 2367 2136
Size: 231 7765
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5090 5124 6187 6221 1903
Size: 34 3153 34 1063 34 3678
Fragments arranged by size:
3678 3153 1063 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5888 7098 5888
Size: 1210 6786
BclI T'GATC_A
Cuts at: 1548 2689 3007 4858 1548
Size: 1141 318 1851 4686
Fragments arranged by size:
4686 1851 1141 318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6607 136
Size: 107 122 71 631 605 4935 1525
Fragments arranged by size:
4935 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2419 7991 2419
Size: 5572 2424
BlpI GC'TnA_GC
Cuts at: 5413 5413
Size: 7996
Bme1580I G_kGCm'C
Cuts at: 1790 2648 3879 3931 4039 4498 6057 7213 1790
Size: 858 1231 52 108 459 1559 1156 2573
Fragments arranged by size:
2573 1559 1231 1156 858 459 108 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2178 5727 6685
Size: 416 53 83 399 24 761 3549 958
Cuts at: 6685 442
Size: 1753
Fragments arranged by size:
3549 1753 958 761 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7996
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4600 4895 4600
Size: 295 7701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2379 2460 2812 6656 1239
Size: 816 324 81 352 3844 2579
Fragments arranged by size:
3844 2579 816 352 324 81
Bpu10I CC'TnA_GC
Cuts at: 2136 2367 5172 2136
Size: 231 2805 4960
Fragments arranged by size:
4960 2805 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2923 5268 6121 6986 7284 2923
Size: 2345 853 865 298 3635
Fragments arranged by size:
3635 2345 865 853 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4632 6659 882
Size: 617 3133 2027 2219
Fragments arranged by size:
3133 2219 2027 617
BsaAI yAC'GTr
Cuts at: 493 1204 5787 493
Size: 711 4583 2702
Fragments arranged by size:
4583 2702 711
BsaBI GATnn'nnATC
Cuts at: 7990 7990
Size: 7996
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5803 6185 6820 275
Size: 53 83 186 936 4270 382 635 1451
Fragments arranged by size:
4270 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6429 6827 7101 7248 7659 6429
Size: 398 274 147 411 6766
Fragments arranged by size:
6766 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5169 5199 1178
Size: 30 165 30 3766 30 3975
Fragments arranged by size:
3975 3766 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2052 2055 3716 3780 3825 4059 4197 4245
Size: 866 3 1661 64 45 234 138 48
Cuts at: 4245 4293 4341 4389 4437 1186
Size: 48 48 48 48 4745
Fragments arranged by size:
4745 1661 866 234 138 64 48 48
48 48 48 45 3
BseYI C'CCAG_C
Cuts at: 2726 7199 7695 2726
Size: 4473 496 3027
Fragments arranged by size:
4473 3027 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2598 3034 4953 1163
Size: 573 862 436 1919 4206
Fragments arranged by size:
4206 1919 862 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5446 6207 7235 1540
Size: 275 186 3445 761 1028 2301
Fragments arranged by size:
3445 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2395 2465 6057 6142 7213 2395
Size: 70 3592 85 1071 3178
Fragments arranged by size:
3592 3178 1071 85 70
BsmI GAATG_Cn'
Cuts at: 5507 5600 5507
Size: 93 7903
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2465 2648 3879 3931 4039 4498
Size: 399 605 70 183 1231 52 108 459
Cuts at: 4498 5110 5315 6057 6142 7213 1391
Size: 612 205 742 85 1071 2174
Fragments arranged by size:
2174 1231 1071 742 612 605 459 399
205 183 108 85 70 52
BspEI T'CCGG_A
Cuts at: 7659 7659
Size: 7996
BspHI T'CATG_A
Cuts at: 5885 7615 5885
Size: 1730 6266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4135 4159 5351 844
Size: 3291 24 1192 3489
Fragments arranged by size:
3489 3291 1192 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2444 5883 1394
Size: 429 621 3439 3507
Fragments arranged by size:
3507 3439 621 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6491 6665 65
Size: 1183 5243 174 1396
Fragments arranged by size:
5243 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2236 6640 6827 1700
Size: 110 426 4404 187 2869
Fragments arranged by size:
4404 2869 426 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7996
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7996
BssSI C'ACGA_G
Cuts at: 6056 7068 6056
Size: 1012 6984
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2390 794
Size: 1596 6400
BstBI TT'CG_AA
Cuts at: 6800 6800
Size: 7996
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7996
BstXI CCAn_nnnn'nTGG
Cuts at: 4171 4219 4267 4315 4363 4171
Size: 48 48 48 48 7804
Fragments arranged by size:
7804 48 48 48 48
BstYI r'GATC_y
Cuts at: 1972 2419 3815 4579 5326 5693 6078 6095 7547
Size: 447 1396 764 747 367 385 17 1452
Cuts at: 7547 7633 7645 7991 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1396 764 747 447 385 367
346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2830 2830
Size: 7996
BtgI C'CryG_G
Cuts at: 513 1065 2004 3161 3903 4011 4074 4119 4212
Size: 552 939 1157 742 108 63 45 93
Cuts at: 4212 4260 4356 513
Size: 48 96 4153
Fragments arranged by size:
4153 1157 939 742 552 108 96 93
63 48 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7257
Fragments arranged by size:
7257 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2616 4601 4962 5512 6306 6326 2065
Size: 551 1985 361 550 794 20 3735
Fragments arranged by size:
3735 1985 794 551 550 361 20
ClaI AT'CG_AT
Cuts at: 1554 2683 1554
Size: 1129 6867
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7961
DraI TTT'AAA
Cuts at: 3001 4806 5300 3001
Size: 1805 494 5697
Fragments arranged by size:
5697 1805 494
DraIII CAC_nnn'GTG
Cuts at: 3101 3101
Size: 7996
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7003 809
Size: 507 5687 1802
Fragments arranged by size:
5687 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2127 5111
Size: 54 1425 50 275 18 177 120 2984
Cuts at: 5111 5443 6332 7961 8
Size: 332 889 1629 43
Fragments arranged by size:
2984 1629 1425 889 332 275 177 120
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5443 1537
Size: 275 3631 4090
Fragments arranged by size:
4090 3631 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 2565 2617 2619 4620 4689 4698 5926
Size: 927 285 52 2 2001 69 9 1228
Cuts at: 5926 1353
Size: 3423
Fragments arranged by size:
3423 2001 1228 927 285 69 52 9
2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6583 6957 7103 387
Size: 1064 138 4994 374 146 1280
Fragments arranged by size:
4994 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2463 2463
Size: 7996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2379 2460 2535 2812 4670 4917
Size: 393 423 324 81 75 277 1858 247
Cuts at: 4917 6059 6656 7443 7711 1239
Size: 1142 597 787 268 1524
Fragments arranged by size:
1858 1524 1142 787 597 423 393 324
277 268 247 81 75
EcoO109I rG'GnC_Cy
Cuts at: 2644 2645 3845 3875 3983 4109 2644
Size: 1 1200 30 108 126 6531
Fragments arranged by size:
6531 1200 126 108 30 1
EcoRI G'AATT_C
Cuts at: 5305 5683 5305
Size: 378 7618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4700 5170 4700
Size: 470 7526
FspI TGC'GCA
Cuts at: 6502 6502
Size: 7996
HaeII r_GCGC'y
Cuts at: 1678 7143 1678
Size: 5465 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2802 3602 3634 3800
Size: 32 135 32 1198 32 800 32 166
Cuts at: 3800 3817 3832 3849 5057 5089 6637 6669 6711
Size: 17 15 17 1208 32 1548 32 42
Cuts at: 6711 6743 1373
Size: 32 2626
Fragments arranged by size:
2626 1548 1208 1198 800 166 135 42
32 32 32 32 32 32 32 17
17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3580 5340 5586 669
Size: 988 281 1642 1760 246 3079
Fragments arranged by size:
3079 1760 1642 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7996
HpaI GTT'AAC
Cuts at: 5586 5586
Size: 7996
KpnI G_GTAC'C
Cuts at: 5321 5321
Size: 7996
MfeI C'AATT_G
Cuts at: 3172 5595 3172
Size: 2423 5573
MluI A'CGCG_T
Cuts at: 7872 7872
Size: 7996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7110 7294 1305
Size: 5805 184 2007
Fragments arranged by size:
5805 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 5997
Fragments arranged by size:
5997 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2774 2917 2985 3001 4169 4217
Size: 648 906 702 143 68 16 1168 48
Cuts at: 4217 4265 4313 4361 5043 5954 6313 6472 518
Size: 48 48 48 682 911 359 159 2042
Fragments arranged by size:
2042 1168 911 906 702 682 648 359
159 143 68 48 48 48 48 16
MspA1I CmG'CkG
Cuts at: 1606 2187 2664 5412 6089 7237 7482 1606
Size: 581 477 2748 677 1148 245 2120
Fragments arranged by size:
2748 2120 1148 677 581 477 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3903 4074 4119 4212 4260 4356
Size: 552 939 1899 171 45 93 48 96
Cuts at: 4356 513
Size: 4153
Fragments arranged by size:
4153 1899 939 552 171 96 93 48
45
NdeI CA'TA_TG
Cuts at: 387 2970 387
Size: 2583 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7996
NotI GC'GGCC_GC
Cuts at: 5443 5443
Size: 7996
NspI r_CATG'y
Cuts at: 1132 1229 2095 2838 2888 3143 3155 3914 4483
Size: 97 866 743 50 255 12 759 569
Cuts at: 4483 5074 5354 6899 1132
Size: 591 280 1545 2229
Fragments arranged by size:
2229 1545 866 759 743 591 569 280
255 97 50 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7996
PciI A'CATG_T
Cuts at: 2091 2834 3139 3151 5070 6895 2091
Size: 743 305 12 1919 1825 3192
Fragments arranged by size:
3192 1919 1825 743 305 12
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2020 1108
Size: 912 7084
PmeI GTTT'AAAC
Cuts at: 5300 5300
Size: 7996
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6065 6097 7578 7610 6065
Size: 32 1481 32 6451
Fragments arranged by size:
6451 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7996
PsiI TTA'TAA
Cuts at: 5566 5566
Size: 7996
PspOMI G'GGCC_C
Cuts at: 2644 3875 2644
Size: 1231 6765
PstI C_TGCA'G
Cuts at: 830 2623 5348 830
Size: 1793 2725 3478
Fragments arranged by size:
3478 2725 1793
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7996
PvuII CAG'CTG
Cuts at: 2187 2187
Size: 7996
SacI G_AGCT'C
Cuts at: 2465 2465
Size: 7996
SalI G'TCGA_C
Cuts at: 1936 5338 1936
Size: 3402 4594
SapI GCTCTTCn'nnn_
Cuts at: 2617 2617
Size: 7996
SbfI CC_TGCA'GG
Cuts at: 5348 5348
Size: 7996
ScaI AGT'ACT
Cuts at: 6244 7956 6244
Size: 1712 6284
SexAI A'CCwGG_T
Cuts at: 2199 4723 2199
Size: 2524 5472
SfcI C'TryA_G
Cuts at: 826 1045 2619 3215 5344 6479 7160 7351 826
Size: 219 1574 596 2129 1135 681 191 1471
Fragments arranged by size:
2129 1574 1471 1135 681 596 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7996
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7996
SmaI CCC'GGG
Cuts at: 1872 5323 1872
Size: 3451 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2938 5283 6100 7001 7263 820
Size: 197 926 995 2345 817 901 262 1553
Fragments arranged by size:
2345 1553 995 926 901 817 262 197
SnaBI TAC'GTA
Cuts at: 493 5787 493
Size: 5294 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7996
SphI G_CATG'C
Cuts at: 1132 3914 5354 1132
Size: 2782 1440 3774
Fragments arranged by size:
3774 2782 1440
SspI AAT'ATT
Cuts at: 5 52 3325 5920 5
Size: 47 3273 2595 2081
Fragments arranged by size:
3273 2595 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7996
StyI C'CwwG_G
Cuts at: 513 1065 2004 2640 2819 3903 4074 4119 4212
Size: 552 939 636 179 1084 171 45 93
Cuts at: 4212 4260 4356 5154 5435 513
Size: 48 96 798 281 3074
Fragments arranged by size:
3074 1084 939 798 636 552 281 179
171 96 93 48 45
SwaI ATTT'AAAT
Cuts at: 3001 3001
Size: 7996
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2027 4022 6023 6040 6193 6378 6823
Size: 1255 447 1995 2001 17 153 185 445
Cuts at: 6823 325
Size: 1498
Fragments arranged by size:
2001 1995 1498 1255 447 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3737 4045 5262
Size: 274 80 33 51 1443 1760 308 1217
Cuts at: 5262 6242 7954 96
Size: 980 1712 138
Fragments arranged by size:
1760 1712 1443 1217 980 308 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7956 7988 7956
Size: 32 7964
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7996
XbaI T'CTAG_A
Cuts at: 5332 5332
Size: 7996
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2122 2723 3593 4969 2017
Size: 105 601 870 1376 5044
Fragments arranged by size:
5044 1376 870 601 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7996
XmaI C'CCGG_G
Cuts at: 1870 5321 1870
Size: 3451 4545
XmnI GAAnn'nnTTC
Cuts at: 3529 6125 3529
Size: 2596 5400
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5804 276
Size: 53 83 186 936 4270 2468
Fragments arranged by size:
4270 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SfiI SfoI SrfI