Restriction map for
pFN21AB5110
(Circular) (Six-base) MAPSORT of: pFN21AB5110.seq Check: 6453 from: 1 to: 6619
pFN21AB5110 6619 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2241 3213 3962 1937
Size: 304 972 749 4594
Fragments arranged by size:
4594 972 749 304
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 2996 3328 4682 6066 6334 1632
Size: 1226 138 332 1354 1384 268 1917
Fragments arranged by size:
1917 1384 1354 1226 332 268 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 2094 5518 6495 1203
Size: 891 3424 977 1327
Fragments arranged by size:
3424 1327 977 891
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 3461 1166
Size: 2295 4324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3479 3603 1184
Size: 2295 124 4200
Fragments arranged by size:
4200 2295 124
AlwNI CAG_nnn'CTG
Cuts at: 2567 2881 3193 4362 5934 2567
Size: 314 312 1169 1572 3252
Fragments arranged by size:
3252 1572 1169 314 312
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 2417 3770 3928 4154 4306 6415 1493
Size: 924 1353 158 226 152 2109 1697
Fragments arranged by size:
2109 1697 1353 924 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 2739 3944 1870
Size: 73 796 1205 4545
Fragments arranged by size:
4545 1205 796 73
BamHI G'GATC_C
Cuts at: 3949 4316 3949
Size: 367 6252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3848 3940 5395 618
Size: 325 217 627 2061 92 1455 1842
Fragments arranged by size:
2061 1842 1455 627 325 217 92
BanII G_rGCy'C
Cuts at: 1391 3938 1391
Size: 2547 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2576 3018 928
Size: 971 677 442 4529
Fragments arranged by size:
4529 971 677 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2894 2928 3817 3851 4810
Size: 34 50 34 873 34 889 34 959
Cuts at: 4810 4844 1903
Size: 34 3678
Fragments arranged by size:
3678 959 889 873 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 3043 1548
Size: 1495 5124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5230 136
Size: 107 122 71 631 605 3558 1525
Fragments arranged by size:
3558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6614 6614
Size: 6619
BlpI GC'TnA_GC
Cuts at: 4036 4036
Size: 6619
Bme1580I G_kGCm'C
Cuts at: 1790 3427 3851 4680 5836 1790
Size: 1637 424 829 1156 2573
Fragments arranged by size:
2573 1637 1156 829 424
BmgBI CAC'GTC
Cuts at: 1318 2260 3550 1318
Size: 942 1290 4387
Fragments arranged by size:
4387 1290 942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2762 2865 2963
Size: 416 53 83 399 24 1345 103 98
Cuts at: 2963 4350 5308 442
Size: 1387 958 1753
Fragments arranged by size:
1753 1387 1345 958 416 399 103 98
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3420 5279 1239
Size: 2181 1859 2579
Fragments arranged by size:
2579 2181 1859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 3022 4744 5609 5907 2150
Size: 872 1722 865 298 2862
Fragments arranged by size:
2862 1722 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5282 882
Size: 617 3783 2219
Fragments arranged by size:
3783 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3533 4410 493
Size: 711 2329 877 2702
Fragments arranged by size:
2702 2329 877 711
BsaBI GATnn'nnATC
Cuts at: 6613 6613
Size: 6619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4426 4808 5443 275
Size: 53 83 186 936 2893 382 635 1451
Fragments arranged by size:
2893 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2081 5052 5450 5724 5871 6282 2081
Size: 2971 398 274 147 411 2418
Fragments arranged by size:
2971 2418 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6394
Fragments arranged by size:
6394 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2639 2663 1186
Size: 1207 246 24 5142
Fragments arranged by size:
5142 1207 246 24
BseYI C'CCAG_C
Cuts at: 3240 3380 5822 6318 3240
Size: 140 2442 496 3541
Fragments arranged by size:
3541 2442 496 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3206 3485 1163
Size: 573 1470 279 4297
Fragments arranged by size:
4297 1470 573 279
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4069 4830 5858 1540
Size: 275 186 2068 761 1028 2301
Fragments arranged by size:
2301 2068 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2401 4680 4765 5836 2049
Size: 352 2279 85 1071 2832
Fragments arranged by size:
2832 2279 1071 352 85
BsmI GAATG_Cn'
Cuts at: 4130 4223 4130
Size: 93 6526
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2401 3427 3851 3938 4680 4765
Size: 399 259 352 1026 424 87 742 85
Cuts at: 4765 5836 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1026 742 424 399 352 259
87 85
BspEI T'CCGG_A
Cuts at: 6282 6282
Size: 6619
BspHI T'CATG_A
Cuts at: 3909 4508 6238 3909
Size: 599 1730 4290
Fragments arranged by size:
4290 1730 599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3974 844
Size: 3130 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4506 1394
Size: 429 2683 3507
Fragments arranged by size:
3507 2683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3051 3075 3354 3504 3519 3679 5114
Size: 1183 1803 24 279 150 15 160 1435
Cuts at: 5114 5288 65
Size: 174 1396
Fragments arranged by size:
1803 1435 1396 1183 279 174 160 150
24 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 3851 5263 5450 1700
Size: 110 2041 1412 187 2869
Fragments arranged by size:
2869 2041 1412 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3077 3193 794
Size: 2283 116 4220
Fragments arranged by size:
4220 2283 116
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstXI CCAn_nnnn'nTGG
Cuts at: 3112 3895 3112
Size: 783 5836
BstYI r'GATC_y
Cuts at: 1972 2220 2295 2391 2878 3949 4316 4701 4718
Size: 248 75 96 487 1071 367 385 17
Cuts at: 4718 6170 6256 6268 6614 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1071 487 385 367 346 248
96 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 2814 2814
Size: 6619
BtgI C'CryG_G
Cuts at: 513 1065 3387 3459 3510 3681 513
Size: 552 2322 72 51 171 3451
Fragments arranged by size:
3451 2322 552 171 72 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 4135 4929 4949 4135
Size: 794 20 5805
Fragments arranged by size:
5805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 2957 2957
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3080 3692 5626 809
Size: 507 1764 612 1934 1802
Fragments arranged by size:
1934 1802 1764 612 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 3609 3853
Size: 54 1425 50 275 18 1621 158 244
Cuts at: 3853 4066 4955 6584 8
Size: 213 889 1629 43
Fragments arranged by size:
1629 1621 1425 889 275 244 213 158
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4549 1353
Size: 3196 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2858 2996 3328 3420 4682 5279 6066
Size: 393 1226 138 332 92 1262 597 787
Cuts at: 6066 6334 1239
Size: 268 1524
Fragments arranged by size:
1524 1262 1226 787 597 393 332 268
138 92
EcoNI CCTnn'n_nnAGG
Cuts at: 3911 3911
Size: 6619
EcoO109I rG'GnC_Cy
Cuts at: 2594 2980 2594
Size: 386 6233
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3337 3337
Size: 6619
FspI TGC'GCA
Cuts at: 5125 5125
Size: 6619
HaeII r_GCGC'y
Cuts at: 1678 3256 5766 1678
Size: 1578 2510 2531
Fragments arranged by size:
2531 2510 1578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2383 2415 2989 3005 3021
Size: 32 135 32 811 32 574 16 16
Cuts at: 3021 3028 3037 3060 5260 5292 5334 5366 1373
Size: 7 9 23 2200 32 42 32 2626
Fragments arranged by size:
2626 2200 811 574 135 42 32 32
32 32 32 23 16 16 9 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 3963 4209 669
Size: 988 281 154 1871 246 3079
Fragments arranged by size:
3079 1871 988 281 246 154
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6619
HpaI GTT'AAC
Cuts at: 2092 4209 2092
Size: 2117 4502
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 2094 6495 2094
Size: 4401 2218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2323 2712 2760 3093 5733 5917 1305
Size: 1018 389 48 333 2640 184 2007
Fragments arranged by size:
2640 2007 1018 389 333 184 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3611 10
Size: 54 1425 343 1779 3018
Fragments arranged by size:
3018 1779 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3461 3509 4577 4936 5095 518
Size: 648 1242 1053 48 1068 359 159 2042
Fragments arranged by size:
2042 1242 1068 1053 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 3397 3691 4035 4712 5860 6105 1606
Size: 1791 294 344 677 1148 245 2120
Fragments arranged by size:
2120 1791 1148 677 344 294 245
NcoI C'CATG_G
Cuts at: 513 1065 3459 3510 513
Size: 552 2394 51 3622
Fragments arranged by size:
3622 2394 552 51
NdeI CA'TA_TG
Cuts at: 387 2236 387
Size: 1849 4770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 2965 3220 3580 3977 5522 1132
Size: 97 1736 255 360 397 1545 2229
Fragments arranged by size:
2229 1736 1545 397 360 255 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6619
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3727 1108
Size: 2619 4000
PfoI T'CCnGG_A
Cuts at: 3222 3222
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4688 4720 6201 6233 4688
Size: 32 1481 32 5074
Fragments arranged by size:
5074 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2594 2594
Size: 6619
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6619
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PstI C_TGCA'G
Cuts at: 830 2121 2572 2891 3073 3198 3971 830
Size: 1291 451 319 182 125 773 3478
Fragments arranged by size:
3478 1291 773 451 319 182 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 3397 3691 3397
Size: 294 6325
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 2791 4867 6579 2791
Size: 2076 1712 2831
Fragments arranged by size:
2831 2076 1712
SfcI C'TryA_G
Cuts at: 826 1045 2117 2568 2887 3069 3170 3194 3967
Size: 219 1072 451 319 182 101 24 773
Cuts at: 3967 5102 5783 5974 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 773 681 451 319 219
191 182 101 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 2741 3946 1872
Size: 869 1205 4545
Fragments arranged by size:
4545 1205 869
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 3001 4723 5624 5886 820
Size: 197 926 222 836 1722 901 262 1553
Fragments arranged by size:
1722 1553 926 901 836 262 222 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 3580 3977 1132
Size: 2448 397 3774
Fragments arranged by size:
3774 2448 397
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6619
StyI C'CwwG_G
Cuts at: 513 1065 2031 2484 2943 3459 3510 3538 3711
Size: 552 966 453 459 516 51 28 173
Cuts at: 3711 3888 4058 513
Size: 177 170 3074
Fragments arranged by size:
3074 966 552 516 459 453 177 173
170 51 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 2744 2945 4646 4663 4816 5001
Size: 1255 582 582 201 1701 17 153 185
Cuts at: 5001 5446 325
Size: 445 1498
Fragments arranged by size:
1701 1498 1255 582 582 445 201 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2789 4865 6577
Size: 274 80 33 51 1443 812 2076 1712
Cuts at: 6577 96
Size: 138
Fragments arranged by size:
2076 1712 1443 812 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6579 6611 6579
Size: 32 6587
Tth111I GACn'n_nGTC
Cuts at: 1316 2857 1316
Size: 1541 5078
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2950 3841 3895 2950
Size: 891 54 5674
Fragments arranged by size:
5674 891 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 2739 3944 1870
Size: 869 1205 4545
Fragments arranged by size:
4545 1205 869
XmnI GAAnn'nnTTC
Cuts at: 3341 4748 3341
Size: 1407 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI