Restriction map for
pFN21AB5109
(Circular) (Six-base) MAPSORT of: pFN21AB5109.seq Check: 5640 from: 1 to: 6523
pFN21AB5109 6523 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2035 2886 2035
Size: 851 5672
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2793 4333 278
Size: 53 83 186 936 1257 1540 2468
Fragments arranged by size:
2468 1540 1257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3866 1937
Size: 1929 4594
Acc65I G'GTAC_C
Cuts at: 3844 3844
Size: 6523
AclI AA'CG_TT
Cuts at: 1563 4650 5023 1563
Size: 3087 373 3063
Fragments arranged by size:
3087 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2358 3304 3320 3461 4586 5970 6238 1632
Size: 726 946 16 141 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 946 726 268 141 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6326
AflIII A'CryG_T
Cuts at: 1203 5422 6399 1203
Size: 4219 977 1327
Fragments arranged by size:
4219 1327 977
AgeI A'CCGG_T
Cuts at: 5354 5354
Size: 6523
AhdI GACnn_n'nnGTC
Cuts at: 3290 5252 3290
Size: 1962 4561
AleI CACnn'nnGTG
Cuts at: 1166 2706 1166
Size: 1540 4983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2765 2957 1184
Size: 1581 192 4750
Fragments arranged by size:
4750 1581 192
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 6491
AlwNI CAG_nnn'CTG
Cuts at: 2851 2980 4266 5838 2851
Size: 129 1286 1572 3536
Fragments arranged by size:
3536 1572 1286 129
ApaI G_GGCC'C
Cuts at: 3476 3476
Size: 6523
ApaLI G'TGCA_C
Cuts at: 3362 4580 5736 3362
Size: 1218 1156 4149
Fragments arranged by size:
4149 1218 1156
ApoI r'AATT_y
Cuts at: 1493 3832 4058 4210 6319 1493
Size: 2339 226 152 2109 1697
Fragments arranged by size:
2339 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3042 5077 160
Size: 872 2010 2035 1606
Fragments arranged by size:
2035 2010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
AvaI C'yCGr_G
Cuts at: 1870 1943 3254 3848 1870
Size: 73 1311 594 4545
Fragments arranged by size:
4545 1311 594 73
BamHI G'GATC_C
Cuts at: 3853 4220 3853
Size: 367 6156
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3431 3506 3844 5299 618
Size: 325 217 627 1644 75 338 1455 1842
Fragments arranged by size:
1842 1644 1455 627 338 325 217 75
BanII G_rGCy'C
Cuts at: 1391 2831 3348 3476 3842 1391
Size: 1440 517 128 366 4072
Fragments arranged by size:
4072 1440 517 366 128
BbeI G_GCGC'C
Cuts at: 3510 3510
Size: 6523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2346 2386 2666 928
Size: 971 447 40 280 4785
Fragments arranged by size:
4785 971 447 280 40
BbvCI CC'TCA_GC
Cuts at: 2249 2249
Size: 6523
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4714 4748 1903
Size: 34 50 34 2693 34 3678
Fragments arranged by size:
3678 2693 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2254 3254 4415 5625 2254
Size: 1000 1161 1210 3152
Fragments arranged by size:
3152 1210 1161 1000
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6523
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2485 5134 136
Size: 107 122 71 631 605 813 2649 1525
Fragments arranged by size:
2649 1525 813 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6518 6518
Size: 6523
BlpI GC'TnA_GC
Cuts at: 3940 3940
Size: 6523
Bme1580I G_kGCm'C
Cuts at: 1790 3366 3434 3476 4584 5740 1790
Size: 1576 68 42 1108 1156 2573
Fragments arranged by size:
2573 1576 1156 1108 68 42
BmgBI CAC'GTC
Cuts at: 1318 2947 3487 1318
Size: 1629 540 4354
Fragments arranged by size:
4354 1629 540
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2673 3058 4254
Size: 416 53 83 399 24 1256 385 1196
Cuts at: 4254 5212 442
Size: 958 1753
Fragments arranged by size:
1753 1256 1196 958 416 399 385 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6523
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 6523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5183 1239
Size: 3944 2579
Bpu10I CC'TnA_GC
Cuts at: 2249 2249
Size: 6523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4648 5513 5811 4648
Size: 865 298 5360
Fragments arranged by size:
5360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5186 882
Size: 617 3687 2219
Fragments arranged by size:
3687 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4314 493
Size: 711 3110 2702
Fragments arranged by size:
3110 2702 711
BsaBI GATnn'nnATC
Cuts at: 2645 6517 2645
Size: 3872 2651
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2790 3507 3528
Size: 53 83 186 936 525 732 717 21
Cuts at: 3528 4330 4712 5347 275
Size: 802 382 635 1451
Fragments arranged by size:
1451 936 802 732 717 635 525 382
186 83 53 21
BsaWI w'CCGG_w
Cuts at: 2228 3650 4956 5354 5628 5775 6186 2228
Size: 1422 1306 398 274 147 411 2565
Fragments arranged by size:
2565 1422 1306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2210 2240 2267 2297 2660
Size: 30 165 30 807 30 27 30 363
Cuts at: 2660 2690 3632 3662 1178
Size: 30 942 30 4039
Fragments arranged by size:
4039 942 807 363 165 30 30 30
30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6523
BseYI C'CCAG_C
Cuts at: 2146 2207 2309 2683 2851 2930 3083 5726 6222
Size: 61 102 374 168 79 153 2643 496
Cuts at: 6222 2146
Size: 2447
Fragments arranged by size:
2643 2447 496 374 168 153 102 79
61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2927 3716 1163
Size: 573 1191 789 3970
Fragments arranged by size:
3970 1191 789 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3973 4734 5762 1540
Size: 275 186 1972 761 1028 2301
Fragments arranged by size:
2301 1972 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2280 3366 3768 4584 4669 5740 2175
Size: 105 1086 402 816 85 1071 2958
Fragments arranged by size:
2958 1086 1071 816 402 105 85
BsiWI C'GTAC_G
Cuts at: 2010 2010
Size: 6523
BsmI GAATG_Cn'
Cuts at: 2519 4034 4127 2519
Size: 1515 93 4915
Fragments arranged by size:
4915 1515 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2065 3053 2065
Size: 988 5535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2280 2831 3348 3366 3434 3476
Size: 399 385 105 551 517 18 68 42
Cuts at: 3476 3768 3842 4584 4669 5740 1391
Size: 292 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 517 399 385 292
105 85 74 68 42 18
BspEI T'CCGG_A
Cuts at: 2228 6186 2228
Size: 3958 2565
BspHI T'CATG_A
Cuts at: 4412 6142 4412
Size: 1730 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2035 2886 3878 844
Size: 1191 851 992 3489
Fragments arranged by size:
3489 1191 992 851
BsrBI CCG'CTC
Cuts at: 1394 1823 4410 1394
Size: 429 2587 3507
Fragments arranged by size:
3507 2587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2220 2325 5018 5192 65
Size: 1183 972 105 2693 174 1396
Fragments arranged by size:
2693 1396 1183 972 174 105
BsrFI r'CCGG_y
Cuts at: 1700 1810 2475 2484 3726 5167 5354 1700
Size: 110 665 9 1242 1441 187 2869
Fragments arranged by size:
2869 1441 1242 665 187 110 9
BsrGI T'GTAC_A
Cuts at: 96 2103 96
Size: 2007 4516
BssHII G'CGCG_C
Cuts at: 1925 2679 1925
Size: 754 5769
BssSI C'ACGA_G
Cuts at: 4583 5595 4583
Size: 1012 5511
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6523
BstBI TT'CG_AA
Cuts at: 5327 5327
Size: 6523
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6523
BstXI CCAn_nnnn'nTGG
Cuts at: 2708 2839 3538 2708
Size: 131 699 5693
Fragments arranged by size:
5693 699 131
BstYI r'GATC_y
Cuts at: 1972 2253 3711 3853 4220 4605 4622 6074 6160
Size: 281 1458 142 367 385 17 1452 86
Cuts at: 6160 6172 6518 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1458 1452 385 367 346 281 142
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2085 2129 2085
Size: 44 6479
BtgI C'CryG_G
Cuts at: 513 1065 2358 2493 2805 2823 2832 3120 3267
Size: 552 1293 135 312 18 9 288 147
Cuts at: 3267 513
Size: 3769
Fragments arranged by size:
3769 1293 552 312 288 147 135 18
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2517 487
Size: 683 56 1291 4493
Fragments arranged by size:
4493 1291 683 56
BtsI GCAGTG_nn'
Cuts at: 3366 4039 4833 4853 3366
Size: 673 794 20 5036
Fragments arranged by size:
5036 794 673 20
ClaI AT'CG_AT
Cuts at: 1554 2969 1554
Size: 1415 5108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6488
DraI TTT'AAA
Cuts at: 3827 3827
Size: 6523
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3407 5530 809
Size: 507 2091 2123 1802
Fragments arranged by size:
2123 2091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2477 2490
Size: 54 1425 50 275 18 363 284 13
Cuts at: 2490 2635 3970 4859 6488 8
Size: 145 1335 889 1629 43
Fragments arranged by size:
1629 1425 1335 889 363 284 275 145
54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3970 1537
Size: 275 2158 4090
Fragments arranged by size:
4090 2158 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2419 4453 1353
Size: 1066 2034 3423
Fragments arranged by size:
3423 2034 1066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2703 3401 5110 5484 5630 387
Size: 1064 138 1114 698 1709 374 146 1280
Fragments arranged by size:
1709 1280 1114 1064 698 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 3304 3320 3461 4586 5183 5970
Size: 393 726 946 16 141 1125 597 787
Cuts at: 5970 6238 1239
Size: 268 1524
Fragments arranged by size:
1524 1125 946 787 726 597 393 268
141 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 3299 2042
Size: 1257 5266
EcoO109I rG'GnC_Cy
Cuts at: 2531 3472 3473 2531
Size: 941 1 5581
Fragments arranged by size:
5581 941 1
EcoRI G'AATT_C
Cuts at: 3832 4210 3832
Size: 378 6145
FspI TGC'GCA
Cuts at: 5029 5029
Size: 6523
HaeII r_GCGC'y
Cuts at: 1678 3510 5670 1678
Size: 1832 2160 2531
Fragments arranged by size:
2531 2160 1832
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2396 2428 2962 2994 3276
Size: 32 135 32 824 32 534 32 282
Cuts at: 3276 3308 3554 3586 3696 3728 5164 5196 5238
Size: 32 246 32 110 32 1436 32 42
Cuts at: 5238 5270 1373
Size: 32 2626
Fragments arranged by size:
2626 1436 824 534 282 246 135 110
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 3867 4113 669
Size: 988 281 643 1286 246 3079
Fragments arranged by size:
3079 1286 988 643 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6523
HpaI GTT'AAC
Cuts at: 4113 4113
Size: 6523
KasI G'GCGC_C
Cuts at: 3506 3506
Size: 6523
KpnI G_GTAC'C
Cuts at: 3848 3848
Size: 6523
MfeI C'AATT_G
Cuts at: 3387 4122 3387
Size: 735 5788
MluI A'CGCG_T
Cuts at: 6399 6399
Size: 6523
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2708 5637 5821 1305
Size: 1403 2929 184 2007
Fragments arranged by size:
2929 2007 1403 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2492 10
Size: 54 1425 343 363 297 4041
Fragments arranged by size:
4041 1425 363 343 297 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2706 3662 3668 4481 4840 4999 518
Size: 648 1540 956 6 813 359 159 2042
Fragments arranged by size:
2042 1540 956 813 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2211 2851 3147 3201 3939 4616 5764 6009
Size: 605 640 296 54 738 677 1148 245
Cuts at: 6009 1606
Size: 2120
Fragments arranged by size:
2120 1148 738 677 640 605 296 245
54
NaeI GCC'GGC
Cuts at: 2477 2477
Size: 6523
NarI GG'CG_CC
Cuts at: 3507 3507
Size: 6523
NcoI C'CATG_G
Cuts at: 513 1065 2823 513
Size: 552 1758 4213
Fragments arranged by size:
4213 1758 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6523
NgoMIV G'CCGG_C
Cuts at: 2475 2475
Size: 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6523
NotI GC'GGCC_GC
Cuts at: 3970 3970
Size: 6523
NspI r_CATG'y
Cuts at: 1132 1229 3881 5426 1132
Size: 97 2652 1545 2229
Fragments arranged by size:
2652 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6523
PciI A'CATG_T
Cuts at: 5422 5422
Size: 6523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 2784 2851 1108
Size: 1077 599 67 4780
Fragments arranged by size:
4780 1077 599 67
PfoI T'CCnGG_A
Cuts at: 3110 3110
Size: 6523
PmeI GTTT'AAAC
Cuts at: 3827 3827
Size: 6523
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4592 4624 6105 6137 4592
Size: 32 1481 32 4978
Fragments arranged by size:
4978 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2531 2531
Size: 6523
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6523
PsiI TTA'TAA
Cuts at: 4093 4093
Size: 6523
PspOMI G'GGCC_C
Cuts at: 3472 3472
Size: 6523
PstI C_TGCA'G
Cuts at: 830 3152 3875 830
Size: 2322 723 3478
Fragments arranged by size:
3478 2322 723
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6523
PvuII CAG'CTG
Cuts at: 2851 3147 2851
Size: 296 6227
SalI G'TCGA_C
Cuts at: 1936 3865 1936
Size: 1929 4594
SanDI GG'GwC_CC
Cuts at: 2531 2531
Size: 6523
SapI GCTCTTCn'nnn_
Cuts at: 2419 2419
Size: 6523
SbfI CC_TGCA'GG
Cuts at: 3875 3875
Size: 6523
ScaI AGT'ACT
Cuts at: 4771 6483 4771
Size: 1712 4811
SexAI A'CCwGG_T
Cuts at: 3102 3102
Size: 6523
SfcI C'TryA_G
Cuts at: 826 1045 3148 3196 3871 5006 5687 5878 826
Size: 219 2103 48 675 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 675 219 191 48
SfoI GGC'GCC
Cuts at: 3508 3508
Size: 6523
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6523
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6523
SmaI CCC'GGG
Cuts at: 1872 3256 3850 1872
Size: 1384 594 4545
Fragments arranged by size:
4545 1384 594
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4627 5528 5790 820
Size: 197 926 2684 901 262 1553
Fragments arranged by size:
2684 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4314 493
Size: 3821 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6523
SphI G_CATG'C
Cuts at: 1132 3881 1132
Size: 2749 3774
SspI AAT'ATT
Cuts at: 5 52 4447 5
Size: 47 4395 2081
Fragments arranged by size:
4395 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6523
StyI C'CwwG_G
Cuts at: 513 1065 2823 3468 3962 513
Size: 552 1758 645 494 3074
Fragments arranged by size:
3074 1758 645 552 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2759 3497 4550 4567 4720 4905 5350
Size: 1255 1179 738 1053 17 153 185 445
Cuts at: 5350 325
Size: 1498
Fragments arranged by size:
1498 1255 1179 1053 738 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 4769 6481
Size: 274 80 33 51 1443 126 2666 1712
Cuts at: 6481 96
Size: 138
Fragments arranged by size:
2666 1712 1443 274 138 126 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 3252 3284 3632 3664 3673 3705 6483
Size: 32 560 32 348 32 9 32 2778
Cuts at: 6483 6515 2660
Size: 32 2668
Fragments arranged by size:
2778 2668 560 348 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6523
XbaI T'CTAG_A
Cuts at: 3859 3859
Size: 6523
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 3118 2306
Size: 812 5711
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6523
XmaI C'CCGG_G
Cuts at: 1870 3254 3848 1870
Size: 1384 594 4545
Fragments arranged by size:
4545 1384 594
XmnI GAAnn'nnTTC
Cuts at: 2027 3317 4652 2027
Size: 1290 1335 3898
Fragments arranged by size:
3898 1335 1290
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2791 4331 276
Size: 53 83 186 936 1257 1540 2468
Fragments arranged by size:
2468 1540 1257 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI