Restriction map for
pFN21AB5108
(Circular) (Six-base) MAPSORT of: pFN21AB5108.seq Check: 6945 from: 1 to: 7090
pFN21AB5108 7090 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4900 278
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4433 1937
Size: 2496 4594
Acc65I G'GTAC_C
Cuts at: 2025 4411 2025
Size: 2386 4704
AclI AA'CG_TT
Cuts at: 1563 5217 5590 1563
Size: 3654 373 3063
Fragments arranged by size:
3654 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2946 3689 3714 3750 3776 3843 5153 6537
Size: 1314 743 25 36 26 67 1310 1384
Cuts at: 6537 6805 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1314 1310 743 268 67 36
26 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6893
AflIII A'CryG_T
Cuts at: 1203 5989 6966 1203
Size: 4786 977 1327
Fragments arranged by size:
4786 1327 977
AgeI A'CCGG_T
Cuts at: 2264 5921 2264
Size: 3657 3433
AhdI GACnn_n'nnGTC
Cuts at: 5819 5819
Size: 7090
AleI CACnn'nnGTG
Cuts at: 1166 3876 1166
Size: 2710 4380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7090
AlwNI CAG_nnn'CTG
Cuts at: 3494 3515 4833 6405 3494
Size: 21 1318 1572 4179
Fragments arranged by size:
4179 1572 1318 21
ApaI G_GGCC'C
Cuts at: 2146 2146
Size: 7090
ApaLI G'TGCA_C
Cuts at: 5147 6303 5147
Size: 1156 5934
ApoI r'AATT_y
Cuts at: 1493 2369 2615 3203 3350 4381 4399 4625 4777
Size: 876 246 588 147 1031 18 226 152
Cuts at: 4777 6886 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1031 876 588 246 226 152
147 18
AseI AT'TA_AT
Cuts at: 160 1032 5644 160
Size: 872 4612 1606
Fragments arranged by size:
4612 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 4415 1870
Size: 73 175 2297 4545
Fragments arranged by size:
4545 2297 175 73
BamHI G'GATC_C
Cuts at: 4420 4787 4420
Size: 367 6723
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 4411 5866 618
Size: 325 217 627 238 2386 1455 1842
Fragments arranged by size:
2386 1842 1455 627 325 238 217
BanII G_rGCy'C
Cuts at: 1391 2146 3446 3916 4409 1391
Size: 755 1300 470 493 4072
Fragments arranged by size:
4072 1300 755 493 470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2583 928
Size: 971 684 5435
Fragments arranged by size:
5435 971 684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5281 5315 1903
Size: 34 3344 34 3678
Fragments arranged by size:
3678 3344 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4982 6192 4982
Size: 1210 5880
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7090
BfrBI ATG'CAT
Cuts at: 2220 2289 2220
Size: 69 7021
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5701 136
Size: 107 122 71 631 605 4029 1525
Fragments arranged by size:
4029 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2478 2762 7085 2478
Size: 284 4323 2483
Fragments arranged by size:
4323 2483 284
BlpI GC'TnA_GC
Cuts at: 2033 3006 4507 2033
Size: 973 1501 4616
Fragments arranged by size:
4616 1501 973
Bme1580I G_kGCm'C
Cuts at: 1790 2146 5151 6307 1790
Size: 356 3005 1156 2573
Fragments arranged by size:
3005 2573 1156 356
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3870 4821 5779
Size: 416 53 83 399 24 2453 951 958
Cuts at: 5779 442
Size: 1753
Fragments arranged by size:
2453 1753 958 951 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5750 1239
Size: 4511 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2215 3752 5215 6080 6378 2215
Size: 1537 1463 865 298 2927
Fragments arranged by size:
2927 1537 1463 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2115 5753 882
Size: 617 616 3638 2219
Fragments arranged by size:
3638 2219 617 616
BsaAI yAC'GTr
Cuts at: 493 1204 4881 493
Size: 711 3677 2702
Fragments arranged by size:
3677 2702 711
BsaBI GATnn'nnATC
Cuts at: 3321 7084 3321
Size: 3763 3327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4897 5279 5914 275
Size: 53 83 186 936 3364 382 635 1451
Fragments arranged by size:
3364 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2264 5523 5921 6195 6342 6753 2264
Size: 3259 398 274 147 411 2601
Fragments arranged by size:
3259 2601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2091 2121 3097 3127 1178
Size: 30 165 30 688 30 976 30 5141
Fragments arranged by size:
5141 976 688 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7090
BseYI C'CCAG_C
Cuts at: 3398 6293 6789 3398
Size: 2895 496 3699
Fragments arranged by size:
3699 2895 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3672 3825 1163
Size: 573 1936 153 4428
Fragments arranged by size:
4428 1936 573 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4540 5301 6329 1540
Size: 275 186 2539 761 1028 2301
Fragments arranged by size:
2539 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2797 2844 5151 5236 6307 2797
Size: 47 2307 85 1071 3580
Fragments arranged by size:
3580 2307 1071 85 47
BsiWI C'GTAC_G
Cuts at: 2065 2065
Size: 7090
BsmI GAATG_Cn'
Cuts at: 4367 4601 4694 4367
Size: 234 93 6763
Fragments arranged by size:
6763 234 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2797 2844 3446 3916 4409 5151
Size: 399 356 651 47 602 470 493 742
Cuts at: 5151 5236 6307 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 602 493 470 399
356 85 47
BspEI T'CCGG_A
Cuts at: 6753 6753
Size: 7090
BspHI T'CATG_A
Cuts at: 4029 4979 6709 4029
Size: 950 1730 4410
Fragments arranged by size:
4410 1730 950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4445 844
Size: 3601 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 4977 1394
Size: 429 190 2964 3507
Fragments arranged by size:
3507 2964 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 3904 4101 5585 5759 65
Size: 1183 2656 197 1484 174 1396
Fragments arranged by size:
2656 1484 1396 1183 197 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2264 5734 5921 1700
Size: 110 218 236 3470 187 2869
Fragments arranged by size:
3470 2869 236 218 187 110
BsrGI T'GTAC_A
Cuts at: 96 4282 96
Size: 4186 2904
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7090
BssSI C'ACGA_G
Cuts at: 2054 5150 6162 2054
Size: 3096 1012 2982
Fragments arranged by size:
3096 2982 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2636 794
Size: 1842 5248
BstBI TT'CG_AA
Cuts at: 5894 5894
Size: 7090
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7090
BstYI r'GATC_y
Cuts at: 1972 2478 2762 3409 3452 3478 4420 4787 5172
Size: 506 284 647 43 26 942 367 385
Cuts at: 5172 5189 6641 6727 6739 7085 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 942 647 506 385 367 346
284 86 43 26 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3534 513
Size: 552 2469 4069
Fragments arranged by size:
4069 2469 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6351
Fragments arranged by size:
6351 683 56
BtsI GCAGTG_nn'
Cuts at: 2403 2469 4606 5400 5420 2403
Size: 66 2137 794 20 4073
Fragments arranged by size:
4073 2137 794 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7090
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3220 3255 529
Size: 35 2656 35 4364
Fragments arranged by size:
4364 2656 35 35
DraI TTT'AAA
Cuts at: 4394 4394
Size: 7090
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3063 6097 809
Size: 507 1747 3034 1802
Fragments arranged by size:
3034 1802 1747 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 3537 4009
Size: 54 1425 50 275 18 361 1346 472
Cuts at: 4009 4537 5426 7055 8
Size: 528 889 1629 43
Fragments arranged by size:
1629 1425 1346 889 528 472 361 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4537 1537
Size: 275 2725 4090
Fragments arranged by size:
4090 2725 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3451 3690 5020 1353
Size: 2098 239 1330 3423
Fragments arranged by size:
3423 2098 1330 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2082 5677 6051 6197 387
Size: 1064 138 493 3595 374 146 1280
Fragments arranged by size:
3595 1280 1064 493 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2946 3689 3714 3750 3776 3843 5153
Size: 393 1314 743 25 36 26 67 1310
Cuts at: 5153 5750 6537 6805 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1314 1310 787 743 597 393 268
67 36 26 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3486 3486
Size: 7090
EcoO109I rG'GnC_Cy
Cuts at: 2071 2143 2071
Size: 72 7018
EcoRI G'AATT_C
Cuts at: 4399 4777 4399
Size: 378 6712
FspI TGC'GCA
Cuts at: 5596 5596
Size: 7090
HaeII r_GCGC'y
Cuts at: 1678 6237 1678
Size: 4559 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2747 2779 3013 3045 3730
Size: 32 135 32 1175 32 234 32 685
Cuts at: 3730 3762 4011 4043 5731 5763 5805 5837 1373
Size: 32 249 32 1688 32 42 32 2626
Fragments arranged by size:
2626 1688 1175 685 249 234 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2505 3288 3708 4434 4680 669
Size: 988 281 567 783 420 726 246 3079
Fragments arranged by size:
3079 988 783 726 567 420 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7090
HpaI GTT'AAC
Cuts at: 3288 3708 4680 3288
Size: 420 972 5698
Fragments arranged by size:
5698 972 420
KpnI G_GTAC'C
Cuts at: 2029 4415 2029
Size: 2386 4704
MfeI C'AATT_G
Cuts at: 3222 3997 4689 3222
Size: 775 692 5623
Fragments arranged by size:
5623 775 692
MluI A'CGCG_T
Cuts at: 6966 6966
Size: 7090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2830 6204 6388 1305
Size: 1525 3374 184 2007
Fragments arranged by size:
3374 2007 1525 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2193 4011 10
Size: 54 1425 343 361 1818 3089
Fragments arranged by size:
3089 1818 1425 361 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3033 3876 5048 5407 5566 518
Size: 648 1867 843 1172 359 159 2042
Fragments arranged by size:
2042 1867 1172 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2459 3581 3973 4506 5183 6331 6576 1606
Size: 853 1122 392 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 1122 853 677 533 392 245
NcoI C'CATG_G
Cuts at: 513 1065 3534 513
Size: 552 2469 4069
Fragments arranged by size:
4069 2469 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7090
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7090
NotI GC'GGCC_GC
Cuts at: 4537 4537
Size: 7090
NsiI A_TGCA'T
Cuts at: 2222 2291 2222
Size: 69 7021
NspI r_CATG'y
Cuts at: 1132 1229 4448 5993 1132
Size: 97 3219 1545 2229
Fragments arranged by size:
3219 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7090
PciI A'CATG_T
Cuts at: 5989 5989
Size: 7090
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2059 1108
Size: 951 6139
PmeI GTTT'AAAC
Cuts at: 4394 4394
Size: 7090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5159 5191 6672 6704 5159
Size: 32 1481 32 5545
Fragments arranged by size:
5545 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7090
PsiI TTA'TAA
Cuts at: 4660 4660
Size: 7090
PspOMI G'GGCC_C
Cuts at: 2142 2142
Size: 7090
PstI C_TGCA'G
Cuts at: 830 2464 4442 830
Size: 1634 1978 3478
Fragments arranged by size:
3478 1978 1634
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7090
PvuII CAG'CTG
Cuts at: 2459 3581 3973 2459
Size: 1122 392 5576
Fragments arranged by size:
5576 1122 392
SalI G'TCGA_C
Cuts at: 1936 4432 1936
Size: 2496 4594
SapI GCTCTTCn'nnn_
Cuts at: 3451 3451
Size: 7090
SbfI CC_TGCA'GG
Cuts at: 4442 4442
Size: 7090
ScaI AGT'ACT
Cuts at: 5338 7050 5338
Size: 1712 5378
SexAI A'CCwGG_T
Cuts at: 2021 3040 3508 2021
Size: 1019 468 5603
Fragments arranged by size:
5603 1019 468
SfcI C'TryA_G
Cuts at: 826 1045 2460 4366 4438 5573 6254 6445 826
Size: 219 1415 1906 72 1135 681 191 1471
Fragments arranged by size:
1906 1471 1415 1135 681 219 191 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7090
SgrAI Cr'CCGG_yG
Cuts at: 1700 2264 1700
Size: 564 6526
SmaI CCC'GGG
Cuts at: 1872 4417 1872
Size: 2545 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2230 3731 5194 6095 6357 820
Size: 197 926 287 1501 1463 901 262 1553
Fragments arranged by size:
1553 1501 1463 926 901 287 262 197
SnaBI TAC'GTA
Cuts at: 493 4881 493
Size: 4388 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7090
SphI G_CATG'C
Cuts at: 1132 4448 1132
Size: 3316 3774
SspI AAT'ATT
Cuts at: 5 52 3601 3657 5014 5
Size: 47 3549 56 1357 2081
Fragments arranged by size:
3549 2081 1357 56 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7090
StyI C'CwwG_G
Cuts at: 513 1065 3281 3534 4529 513
Size: 552 2216 253 995 3074
Fragments arranged by size:
3074 2216 995 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5117 5134 5287 5472 5917 325
Size: 1255 3537 17 153 185 445 1498
Fragments arranged by size:
3537 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4210 4282 5336
Size: 274 80 33 51 1443 2233 72 1054
Cuts at: 5336 7048 96
Size: 1712 138
Fragments arranged by size:
2233 1712 1443 1054 274 138 80 72
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 3097 3129 3463 3495 7050 7082 2091
Size: 32 974 32 334 32 3555 32 2099
Fragments arranged by size:
3555 2099 974 334 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7090
XbaI T'CTAG_A
Cuts at: 4426 4426
Size: 7090
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7090
XmaI C'CCGG_G
Cuts at: 1870 4415 1870
Size: 2545 4545
XmnI GAAnn'nnTTC
Cuts at: 5219 5219
Size: 7090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4898 276
Size: 53 83 186 936 3364 2468
Fragments arranged by size:
3364 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI