Restriction map for
pFN21AB5107
(Circular) (Six-base) MAPSORT of: pFN21AB5107.seq Check: 1725 from: 1 to: 6910
pFN21AB5107 6910 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2544 2544
Size: 6910
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4720 278
Size: 53 83 186 936 3184 2468
Fragments arranged by size:
3184 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4253 1937
Size: 2316 4594
Acc65I G'GTAC_C
Cuts at: 4231 4231
Size: 6910
AclI AA'CG_TT
Cuts at: 1563 5037 5410 1563
Size: 3474 373 3063
Fragments arranged by size:
3474 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2084 2312 2849 2886 3124 3357 3632 3782
Size: 452 228 537 37 238 233 275 150
Cuts at: 3782 4166 4973 6357 6625 1632
Size: 384 807 1384 268 1917
Fragments arranged by size:
1917 1384 807 537 452 384 275 268
238 233 228 150 37
AfeI AGC'GCT
Cuts at: 3112 3305 3112
Size: 193 6717
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6713
AflIII A'CryG_T
Cuts at: 1203 3454 3456 3973 5809 6786 1203
Size: 2251 2 517 1836 977 1327
Fragments arranged by size:
2251 1836 1327 977 517 2
AgeI A'CCGG_T
Cuts at: 5741 5741
Size: 6910
AhdI GACnn_n'nnGTC
Cuts at: 2740 3958 5639 2740
Size: 1218 1681 4011
Fragments arranged by size:
4011 1681 1218
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2292 1184
Size: 1108 5802
AlwNI CAG_nnn'CTG
Cuts at: 2857 4012 4653 6225 2857
Size: 1155 641 1572 3542
Fragments arranged by size:
3542 1572 1155 641
ApaI G_GGCC'C
Cuts at: 2890 3121 2890
Size: 231 6679
ApaLI G'TGCA_C
Cuts at: 3599 4967 6123 3599
Size: 1368 1156 4386
Fragments arranged by size:
4386 1368 1156
ApoI r'AATT_y
Cuts at: 1493 4219 4445 4597 6706 1493
Size: 2726 226 152 2109 1697
Fragments arranged by size:
2726 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5464 160
Size: 872 4432 1606
Fragments arranged by size:
4432 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
AvaI C'yCGr_G
Cuts at: 1870 1943 3653 4235 1870
Size: 73 1710 582 4545
Fragments arranged by size:
4545 1710 582 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3443 3476 3443
Size: 33 6877
BamHI G'GATC_C
Cuts at: 2216 3367 3979 4240 4607 2216
Size: 1151 612 261 367 4519
Fragments arranged by size:
4519 1151 612 367 261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 3196 3748 4231 5686
Size: 325 217 627 481 928 552 483 1455
Cuts at: 5686 618
Size: 1842
Fragments arranged by size:
1842 1455 928 627 552 483 481 325
217
BanII G_rGCy'C
Cuts at: 1391 2031 2196 2780 2890 3121 3287 3714 4229
Size: 640 165 584 110 231 166 427 515
Cuts at: 4229 1391
Size: 4072
Fragments arranged by size:
4072 640 584 515 427 231 166 165
110
BbeI G_GCGC'C
Cuts at: 3752 3752
Size: 6910
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2953 3174 928
Size: 971 325 729 221 4664
Fragments arranged by size:
4664 971 729 325 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5101 5135 1903
Size: 34 50 34 3080 34 3678
Fragments arranged by size:
3678 3080 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4802 6012 4802
Size: 1210 5700
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6910
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2516 3194 5521
Size: 107 122 71 631 605 844 678 2327
Cuts at: 5521 136
Size: 1525
Fragments arranged by size:
2327 1525 844 678 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2133 6905 2133
Size: 4772 2138
BlpI GC'TnA_GC
Cuts at: 3033 4002 4327 3033
Size: 969 325 5616
Fragments arranged by size:
5616 969 325
Bme1580I G_kGCm'C
Cuts at: 1790 2271 2890 3121 3190 3603 4971 6127 1790
Size: 481 619 231 69 413 1368 1156 2573
Fragments arranged by size:
2573 1368 1156 619 481 413 231 69
BmgBI CAC'GTC
Cuts at: 1318 2152 3072 1318
Size: 834 920 5156
Fragments arranged by size:
5156 920 834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2845 3852 4641
Size: 416 53 83 399 24 1428 1007 789
Cuts at: 4641 5599 442
Size: 958 1753
Fragments arranged by size:
1753 1428 1007 958 789 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3817 5570 1239
Size: 2578 1753 2579
Fragments arranged by size:
2579 2578 1753
Bpu10I CC'TnA_GC
Cuts at: 2627 2627
Size: 6910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2111 2183 2552 5035 5900 6198 2006
Size: 105 72 369 2483 865 298 2718
Fragments arranged by size:
2718 2483 865 369 298 105 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2556 2804 5573 882
Size: 617 1057 248 2769 2219
Fragments arranged by size:
2769 2219 1057 617 248
BsaAI yAC'GTr
Cuts at: 493 1204 2315 3259 3457 3976 4107 4701 493
Size: 711 1111 944 198 519 131 594 2702
Fragments arranged by size:
2702 1111 944 711 594 519 198 131
BsaBI GATnn'nnATC
Cuts at: 2138 2361 6904 2138
Size: 223 4543 2144
Fragments arranged by size:
4543 2144 223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 3749 4717 5099
Size: 53 83 186 936 588 1628 968 382
Cuts at: 5099 5734 275
Size: 635 1451
Fragments arranged by size:
1628 1451 968 936 635 588 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2325 2802 5343 5741 6015 6162 6573 2325
Size: 477 2541 398 274 147 411 2662
Fragments arranged by size:
2662 2541 477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3207 3237 1178
Size: 30 165 30 1804 30 4851
Fragments arranged by size:
4851 1804 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2501 2609 3704 1186
Size: 1000 315 108 1095 4392
Fragments arranged by size:
4392 1095 1000 315 108
BseYI C'CCAG_C
Cuts at: 2016 3107 3113 3617 4140 4173 6113 6609 2016
Size: 1091 6 504 523 33 1940 496 2317
Fragments arranged by size:
2317 1940 1091 523 504 496 33 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2522 3188 3536 3566 1163
Size: 573 633 153 666 348 30 4507
Fragments arranged by size:
4507 666 633 573 348 153 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4360 5121 6149 1540
Size: 275 186 2359 761 1028 2301
Fragments arranged by size:
2359 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2864 3603 4971 5056 6127 2196
Size: 668 739 1368 85 1071 2979
Fragments arranged by size:
2979 1368 1071 739 668 85
BsmI GAATG_Cn'
Cuts at: 3807 4421 4514 3807
Size: 614 93 6203
Fragments arranged by size:
6203 614 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2196 2271 2780 2864 2890 3121
Size: 399 241 165 75 509 84 26 231
Cuts at: 3121 3190 3287 3603 3714 4229 4971 5056 6127
Size: 69 97 316 111 515 742 85 1071
Cuts at: 6127 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 515 509 399 316 241
231 165 111 97 85 84 75 69
26
BspEI T'CCGG_A
Cuts at: 2325 6573 2325
Size: 4248 2662
BspHI T'CATG_A
Cuts at: 3717 4799 6529 3717
Size: 1082 1730 4098
Fragments arranged by size:
4098 1730 1082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2544 3997 4265 844
Size: 1700 1453 268 3489
Fragments arranged by size:
3489 1700 1453 268
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2038 3532 4797 1394
Size: 429 188 27 1494 1265 3507
Fragments arranged by size:
3507 1494 1265 429 188 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 5405 5579 65
Size: 1183 4157 174 1396
Fragments arranged by size:
4157 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2913 3155 5554 5741 1700
Size: 110 1103 242 2399 187 2869
Fragments arranged by size:
2869 2399 1103 242 187 110
BsrGI T'GTAC_A
Cuts at: 96 3816 96
Size: 3720 3190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6910
BssSI C'ACGA_G
Cuts at: 2648 3183 4970 5982 2648
Size: 535 1787 1012 3576
Fragments arranged by size:
3576 1787 1012 535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4012 794
Size: 3218 3692
BstBI TT'CG_AA
Cuts at: 5714 5714
Size: 6910
BstEII G'GTnAC_C
Cuts at: 1184 4027 1184
Size: 2843 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 3328 3745 3955 4207 3328
Size: 417 210 252 6031
Fragments arranged by size:
6031 417 252 210
BstYI r'GATC_y
Cuts at: 1972 2133 2216 3367 3504 3979 4240 4607 4992
Size: 161 83 1151 137 475 261 367 385
Cuts at: 4992 5009 6461 6547 6559 6905 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1151 475 385 367 346 261
161 137 86 83 17 12
Bsu36I CC'TnA_GG
Cuts at: 2080 2697 3753 2080
Size: 617 1056 5237
Fragments arranged by size:
5237 1056 617
BtgI C'CryG_G
Cuts at: 513 1065 3226 4022 4095 513
Size: 552 2161 796 73 3328
Fragments arranged by size:
3328 2161 796 552 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6171
Fragments arranged by size:
6171 683 56
BtsI GCAGTG_nn'
Cuts at: 3033 3901 4426 5220 5240 3033
Size: 868 525 794 20 4703
Fragments arranged by size:
4703 868 794 525 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6910
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3261 3296 529
Size: 35 2697 35 4143
Fragments arranged by size:
4143 2697 35 35
DraI TTT'AAA
Cuts at: 4214 4214
Size: 6910
DraIII CAC_nnn'GTG
Cuts at: 3211 3211
Size: 6910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5917 809
Size: 507 4601 1802
Fragments arranged by size:
4601 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 2508 3150
Size: 54 1425 50 275 18 203 475 642
Cuts at: 3150 3403 4357 5246 6875 8
Size: 253 954 889 1629 43
Fragments arranged by size:
1629 1425 954 889 642 475 275 253
203 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4357 1537
Size: 275 2545 4090
Fragments arranged by size:
4090 2545 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 3372 4840 1353
Size: 1103 916 1468 3423
Fragments arranged by size:
3423 1468 1103 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5497 5871 6017 387
Size: 1064 138 3908 374 146 1280
Fragments arranged by size:
3908 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 6910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2312 2849 2886 3124 3357 3632
Size: 393 452 228 537 37 238 233 275
Cuts at: 3632 3782 3817 4166 4973 5570 6357 6625 1239
Size: 150 35 349 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 537 452 393 349
275 268 238 233 228 150 37 35
EcoO109I rG'GnC_Cy
Cuts at: 2446 2887 3117 3607 3952 4072 2446
Size: 441 230 490 345 120 5284
Fragments arranged by size:
5284 490 441 345 230 120
EcoRI G'AATT_C
Cuts at: 4219 4597 4219
Size: 378 6532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 3031 4061 2114
Size: 917 1030 4963
Fragments arranged by size:
4963 1030 917
FspI TGC'GCA
Cuts at: 3665 5416 3665
Size: 1751 5159
HaeII r_GCGC'y
Cuts at: 1678 3114 3307 3752 3769 3994 6057 1678
Size: 1436 193 445 17 225 2063 2531
Fragments arranged by size:
2531 2063 1436 445 225 193 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2726 2758 3056 3088 3944
Size: 32 135 32 1154 32 298 32 856
Cuts at: 3944 3976 5551 5583 5625 5657 1373
Size: 32 1575 32 42 32 2626
Fragments arranged by size:
2626 1575 1154 856 298 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2707 4254 4500 669
Size: 988 281 769 1547 246 3079
Fragments arranged by size:
3079 1547 988 769 281 246
HindIII A'AGCT_T
Cuts at: 748 4043 748
Size: 3295 3615
HpaI GTT'AAC
Cuts at: 4500 4500
Size: 6910
KasI G'GCGC_C
Cuts at: 3748 3748
Size: 6910
KpnI G_GTAC'C
Cuts at: 4235 4235
Size: 6910
MfeI C'AATT_G
Cuts at: 3273 4509 3273
Size: 1236 5674
MluI A'CGCG_T
Cuts at: 6786 6786
Size: 6910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6024 6208 1305
Size: 4719 184 2007
Fragments arranged by size:
4719 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3152 3405 10
Size: 54 1425 343 1320 253 3515
Fragments arranged by size:
3515 1425 1320 343 253 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 3326 4868 5227 5386 518
Size: 648 1108 1052 1542 359 159 2042
Fragments arranged by size:
2042 1542 1108 1052 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 3127 3694 4060 4144 4177 4326 5003
Size: 450 1071 567 366 84 33 149 677
Cuts at: 5003 6151 6396 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1071 677 567 450 366 245
149 84 33
NarI GG'CG_CC
Cuts at: 3749 3749
Size: 6910
NcoI C'CATG_G
Cuts at: 513 1065 3226 4022 4095 513
Size: 552 2161 796 73 3328
Fragments arranged by size:
3328 2161 796 552 73
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6910
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6910
NotI GC'GGCC_GC
Cuts at: 4357 4357
Size: 6910
NspI r_CATG'y
Cuts at: 1132 1229 2848 2960 4268 5813 1132
Size: 97 1619 112 1308 1545 2229
Fragments arranged by size:
2229 1619 1545 1308 112 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6910
PciI A'CATG_T
Cuts at: 5809 5809
Size: 6910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2979 1108
Size: 1871 5039
PfoI T'CCnGG_A
Cuts at: 3644 3644
Size: 6910
PmeI GTTT'AAAC
Cuts at: 4214 4214
Size: 6910
PmlI CAC'GTG
Cuts at: 3457 3976 3457
Size: 519 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2462 2494 4979 5011 6492 6524 2462
Size: 32 2485 32 1481 32 2848
Fragments arranged by size:
2848 2485 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 3607 3952 4072 2446
Size: 1161 345 120 5284
Fragments arranged by size:
5284 1161 345 120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6910
PsiI TTA'TAA
Cuts at: 4480 4480
Size: 6910
PspOMI G'GGCC_C
Cuts at: 2886 3117 2886
Size: 231 6679
PstI C_TGCA'G
Cuts at: 830 4017 4262 830
Size: 3187 245 3478
Fragments arranged by size:
3478 3187 245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6910
PvuII CAG'CTG
Cuts at: 3127 3694 4060 4144 3127
Size: 567 366 84 5893
Fragments arranged by size:
5893 567 366 84
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 6910
SalI G'TCGA_C
Cuts at: 1936 4252 1936
Size: 2316 4594
SbfI CC_TGCA'GG
Cuts at: 4262 4262
Size: 6910
ScaI AGT'ACT
Cuts at: 5158 6870 5158
Size: 1712 5198
SexAI A'CCwGG_T
Cuts at: 3237 3462 3954 3237
Size: 225 492 6193
Fragments arranged by size:
6193 492 225
SfcI C'TryA_G
Cuts at: 826 1045 4013 4076 4258 5393 6074 6265 826
Size: 219 2968 63 182 1135 681 191 1471
Fragments arranged by size:
2968 1471 1135 681 219 191 182 63
SfoI GGC'GCC
Cuts at: 3750 3750
Size: 6910
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6910
SmaI CCC'GGG
Cuts at: 1872 4237 1872
Size: 2365 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2126 2198 2567 5014 5915
Size: 197 926 78 105 72 369 2447 901
Cuts at: 5915 6177 820
Size: 262 1553
Fragments arranged by size:
2447 1553 926 901 369 262 197 105
78 72
SnaBI TAC'GTA
Cuts at: 493 4701 493
Size: 4208 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6910
SphI G_CATG'C
Cuts at: 1132 2848 4268 1132
Size: 1716 1420 3774
Fragments arranged by size:
3774 1716 1420
SspI AAT'ATT
Cuts at: 5 52 4834 5
Size: 47 4782 2081
Fragments arranged by size:
4782 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 5767
StyI C'CwwG_G
Cuts at: 513 1065 2109 3084 3226 3948 4022 4095 4349
Size: 552 1044 975 142 722 74 73 254
Cuts at: 4349 513
Size: 3074
Fragments arranged by size:
3074 1044 975 722 552 254 142 74
73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2382 2417 3097 4046 4937 4954 5107
Size: 1255 802 35 680 949 891 17 153
Cuts at: 5107 5292 5737 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 949 891 802 680 445 185
153 35 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 3816 5156
Size: 274 80 33 51 1443 708 1131 1340
Cuts at: 5156 6868 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1340 1131 708 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3880 3912 6870 6902 3880
Size: 32 2958 32 3888
Fragments arranged by size:
3888 2958 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2321 1316
Size: 1005 5905
XbaI T'CTAG_A
Cuts at: 4246 4246
Size: 6910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2156 2219 3160 2156
Size: 63 941 5906
Fragments arranged by size:
5906 941 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6910
XmaI C'CCGG_G
Cuts at: 1870 4235 1870
Size: 2365 4545
XmnI GAAnn'nnTTC
Cuts at: 3521 5039 3521
Size: 1518 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4718 276
Size: 53 83 186 936 3184 2468
Fragments arranged by size:
3184 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI
BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacII SanDI SapI
SfiI SrfI SwaI