Restriction map for
pFN21AB5106
(Circular) (Six-base) MAPSORT of: pFN21AB5106.seq Check: 5152 from: 1 to: 7057
pFN21AB5106 7057 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3698 3698
Size: 7057
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4867 278
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2598 4400 1937
Size: 661 1802 4594
Fragments arranged by size:
4594 1802 661
Acc65I G'GTAC_C
Cuts at: 2670 4378 2670
Size: 1708 5349
AclI AA'CG_TT
Cuts at: 1563 4182 5184 5557 1563
Size: 2619 1002 373 3063
Fragments arranged by size:
3063 2619 1002 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 2841 4229 5120 6504 6772 1632
Size: 716 493 1388 891 1384 268 1917
Fragments arranged by size:
1917 1388 1384 891 716 493 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6860
AflIII A'CryG_T
Cuts at: 1203 2903 2905 3445 4311 4336 5956 6933 1203
Size: 1700 2 540 866 25 1620 977 1327
Fragments arranged by size:
1700 1620 1327 977 866 540 25 2
AgeI A'CCGG_T
Cuts at: 5888 5888
Size: 7057
AhdI GACnn_n'nnGTC
Cuts at: 5786 5786
Size: 7057
AleI CACnn'nnGTG
Cuts at: 1166 2906 1166
Size: 1740 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 1184
Size: 1179 5878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 7025
AlwNI CAG_nnn'CTG
Cuts at: 4800 6372 4800
Size: 1572 5485
ApaI G_GGCC'C
Cuts at: 2029 2029
Size: 7057
ApaLI G'TGCA_C
Cuts at: 5114 6270 5114
Size: 1156 5901
ApoI r'AATT_y
Cuts at: 1493 4133 4366 4592 4744 6853 1493
Size: 2640 233 226 152 2109 1697
Fragments arranged by size:
2640 2109 1697 233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5611 160
Size: 872 4579 1606
Fragments arranged by size:
4579 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2918 3454 3567 4116 4382 1870
Size: 73 79 896 536 113 549 266 4545
Fragments arranged by size:
4545 896 549 536 266 113 79 73
BamHI G'GATC_C
Cuts at: 4387 4754 4387
Size: 367 6690
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 2543 2670 3262 4033
Size: 325 217 627 508 248 127 592 771
Cuts at: 4033 4378 5833 618
Size: 345 1455 1842
Fragments arranged by size:
1842 1455 771 627 592 508 345 325
248 217 127
BanII G_rGCy'C
Cuts at: 1391 2029 2039 2522 3792 3831 4376 1391
Size: 638 10 483 1270 39 545 4072
Fragments arranged by size:
4072 1270 638 545 483 39 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3890 928
Size: 971 1991 4095
Fragments arranged by size:
4095 1991 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2065 2099 5248 5282 1903
Size: 34 128 34 3149 34 3678
Fragments arranged by size:
3678 3149 128 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4949 6159 4949
Size: 1210 5847
BclI T'GATC_A
Cuts at: 1548 3214 3516 1548
Size: 1666 302 5089
Fragments arranged by size:
5089 1666 302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 3423 5668
Size: 107 122 71 631 605 406 1345 2245
Cuts at: 5668 136
Size: 1525
Fragments arranged by size:
2245 1525 1345 631 605 406 122 107
71
BglII A'GATC_T
Cuts at: 7052 7052
Size: 7057
BlpI GC'TnA_GC
Cuts at: 3295 4474 3295
Size: 1179 5878
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2788 2925 3265 4036 5118 6274 1790
Size: 239 759 137 340 771 1082 1156 2573
Fragments arranged by size:
2573 1156 1082 771 759 340 239 137
BmgBI CAC'GTC
Cuts at: 1318 4312 1318
Size: 2994 4063
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 2719 4788
Size: 416 53 83 399 24 831 471 2069
Cuts at: 4788 5746 442
Size: 958 1753
Fragments arranged by size:
2069 1753 958 831 471 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7057
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2266 2080
Size: 186 6871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2236 2459 2666 3572 4369 5717 1239
Size: 836 161 223 207 906 797 1348 2579
Fragments arranged by size:
2579 1348 906 836 797 223 207 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2097 4091 4244 5182 6047 6345 2097
Size: 1994 153 938 865 298 2809
Fragments arranged by size:
2809 1994 938 865 298 153
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3740 5720 882
Size: 617 2241 1980 2219
Fragments arranged by size:
2241 2219 1980 617
BsaAI yAC'GTr
Cuts at: 493 1204 2161 2906 2926 4848 493
Size: 711 957 745 20 1922 2702
Fragments arranged by size:
2702 1922 957 745 711 20
BsaBI GATnn'nnATC
Cuts at: 7051 7051
Size: 7057
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2601 4864 5246 5881
Size: 53 83 186 936 1068 2263 382 635
Cuts at: 5881 275
Size: 1451
Fragments arranged by size:
2263 1451 1068 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2415 5490 5888 6162 6309 6720 2415
Size: 3075 398 274 147 411 2752
Fragments arranged by size:
3075 2752 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2744 2774 3435 3465 1178
Size: 30 165 30 1341 30 661 30 4770
Fragments arranged by size:
4770 1341 661 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2765 2949 3139 3172 3596 1186
Size: 1270 309 184 190 33 424 4647
Fragments arranged by size:
4647 1270 424 309 190 184 33
BseYI C'CCAG_C
Cuts at: 2745 2890 3025 3272 4009 4162 6260 6756 2745
Size: 145 135 247 737 153 2098 496 3046
Fragments arranged by size:
3046 2098 737 496 247 153 145 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 2561 2948 3107 3125 4361 1163
Size: 573 765 60 387 159 18 1236 3859
Fragments arranged by size:
3859 1236 765 573 387 159 60 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3288 3697 3798 4507 5268 6296
Size: 275 186 1287 409 101 709 761 1028
Cuts at: 6296 1540
Size: 2301
Fragments arranged by size:
2301 1287 1028 761 709 409 275 186
101
BsiHKAI G_wGCw'C
Cuts at: 2061 3867 3912 4233 5118 5203 6274 2061
Size: 1806 45 321 885 85 1071 2844
Fragments arranged by size:
2844 1806 1071 885 321 85 45
BsiWI C'GTAC_G
Cuts at: 3289 3289
Size: 7057
BsmI GAATG_Cn'
Cuts at: 2951 4568 4661 2951
Size: 1617 93 5347
Fragments arranged by size:
5347 1617 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2039 2061 2522 2788 2925 3265
Size: 399 239 10 22 461 266 137 340
Cuts at: 3265 3792 3831 3867 3912 4036 4233 4376 5118
Size: 527 39 36 45 124 197 143 742
Cuts at: 5118 5203 6274 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 461 399 340 266
239 197 143 137 124 85 45 39
36 22 10
BspEI T'CCGG_A
Cuts at: 2415 6720 2415
Size: 4305 2752
BspHI T'CATG_A
Cuts at: 2607 4275 4946 6676 2607
Size: 1668 671 1730 2988
Fragments arranged by size:
2988 1730 1668 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 3698 4412 844
Size: 1508 1346 714 3489
Fragments arranged by size:
3489 1508 1346 714
BsrBI CCG'CTC
Cuts at: 1394 1823 4944 1394
Size: 429 3121 3507
Fragments arranged by size:
3507 3121 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3312 5552 5726 65
Size: 1183 2064 2240 174 1396
Fragments arranged by size:
2240 2064 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 3413 5701 5888 1700
Size: 110 262 1341 2288 187 2869
Fragments arranged by size:
2869 2288 1341 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7057
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7057
BssSI C'ACGA_G
Cuts at: 3578 5117 6129 3578
Size: 1539 1012 4506
Fragments arranged by size:
4506 1539 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7057
BstBI TT'CG_AA
Cuts at: 3822 5861 3822
Size: 2039 5018
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7057
BstXI CCAn_nnnn'nTGG
Cuts at: 2977 3604 4084 2977
Size: 627 480 5950
Fragments arranged by size:
5950 627 480
BstYI r'GATC_y
Cuts at: 1972 2961 3219 3844 4387 4754 5139 5156 6608
Size: 989 258 625 543 367 385 17 1452
Cuts at: 6608 6694 6706 7052 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 989 625 543 385 367 346
258 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2613 3267 3597 3750 513
Size: 552 939 135 474 654 330 153 3820
Fragments arranged by size:
3820 939 654 552 474 330 153 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2209 2980 3128 487
Size: 683 56 983 771 148 4416
Fragments arranged by size:
4416 983 771 683 148 56
BtsI GCAGTG_nn'
Cuts at: 2293 3934 4095 4573 5367 5387 2293
Size: 1641 161 478 794 20 3963
Fragments arranged by size:
3963 1641 794 478 161 20
ClaI AT'CG_AT
Cuts at: 1554 2466 1554
Size: 912 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7022
DraI TTT'AAA
Cuts at: 4361 4361
Size: 7057
DraIII CAC_nnn'GTG
Cuts at: 3061 3061
Size: 7057
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6064 809
Size: 507 4748 1802
Fragments arranged by size:
4748 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 2458 2967
Size: 54 1425 50 275 18 618 10 509
Cuts at: 2967 3285 3322 3415 3774 3795 4287 4504 5393
Size: 318 37 93 359 21 492 217 889
Cuts at: 5393 7022 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 618 509 492 359 318
275 217 93 54 50 43 37 21
18 10
EagI C'GGCC_G
Cuts at: 1537 1812 3285 3795 4504 1537
Size: 275 1473 510 709 4090
Fragments arranged by size:
4090 1473 709 510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 3465 3819 3966 4987 1353
Size: 1064 1048 354 147 1021 3423
Fragments arranged by size:
3423 1064 1048 1021 354 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2560 3700 5644 6018 6164 387
Size: 1064 138 971 1140 1944 374 146 1280
Fragments arranged by size:
1944 1280 1140 1064 971 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2236 2348 2459 2666 2841 3572
Size: 393 443 161 112 111 207 175 731
Cuts at: 3572 4229 4369 5120 5717 6504 6772 1239
Size: 657 140 751 597 787 268 1524
Fragments arranged by size:
1524 787 751 731 657 597 443 393
268 207 175 161 140 112 111
EcoO109I rG'GnC_Cy
Cuts at: 2026 2634 2940 2026
Size: 608 306 6143
Fragments arranged by size:
6143 608 306
EcoRI G'AATT_C
Cuts at: 4366 4744 4366
Size: 378 6679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2056 2194 2056
Size: 138 6919
FspI TGC'GCA
Cuts at: 2147 3491 5563 2147
Size: 1344 2072 3641
Fragments arranged by size:
3641 2072 1344
FspAI rTGC'GCAy
Cuts at: 2147 3491 2147
Size: 1344 5713
HaeII r_GCGC'y
Cuts at: 1678 6204 1678
Size: 4526 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 3183 3215 3836
Size: 32 135 32 629 32 950 32 621
Cuts at: 3836 3868 5698 5730 5772 5804 1373
Size: 32 1830 32 42 32 2626
Fragments arranged by size:
2626 1830 950 629 621 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2599 4401 4647 669
Size: 988 281 661 1802 246 3079
Fragments arranged by size:
3079 1802 988 661 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7057
HpaI GTT'AAC
Cuts at: 4647 4647
Size: 7057
KpnI G_GTAC'C
Cuts at: 2674 4382 2674
Size: 1708 5349
MfeI C'AATT_G
Cuts at: 2829 3232 4656 2829
Size: 403 1424 5230
Fragments arranged by size:
5230 1424 403
MluI A'CGCG_T
Cuts at: 3445 6933 3445
Size: 3488 3569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3648 3879 4296 6171 6355 1305
Size: 2343 231 417 1875 184 2007
Fragments arranged by size:
2343 2007 1875 417 231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2450 2969 3776 4289 10
Size: 54 1425 343 618 519 807 513 2778
Fragments arranged by size:
2778 1425 807 618 519 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2549 2906 2981 3254 3485 3602
Size: 648 921 462 357 75 273 231 117
Cuts at: 3602 4157 5015 5374 5533 518
Size: 555 858 359 159 2042
Fragments arranged by size:
2042 921 858 648 555 462 359 357
273 231 159 117 75
MspA1I CmG'CkG
Cuts at: 1606 2100 2890 3073 3269 3299 3717 3961 4330
Size: 494 790 183 196 30 418 244 369
Cuts at: 4330 4473 5150 6298 6543 1606
Size: 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 790 677 494 418 369 245
244 196 183 143 30
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 7057
NcoI C'CATG_G
Cuts at: 513 1065 2004 3597 3750 513
Size: 552 939 1593 153 3820
Fragments arranged by size:
3820 1593 939 552 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7057
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 7057
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7057
NotI GC'GGCC_GC
Cuts at: 4504 4504
Size: 7057
NspI r_CATG'y
Cuts at: 1132 1229 4021 4340 4415 5960 1132
Size: 97 2792 319 75 1545 2229
Fragments arranged by size:
2792 2229 1545 319 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7057
PciI A'CATG_T
Cuts at: 4336 5956 4336
Size: 1620 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 1108
Size: 1095 5962
PfoI T'CCnGG_A
Cuts at: 2052 3549 2052
Size: 1497 5560
PmeI GTTT'AAAC
Cuts at: 4361 4361
Size: 7057
PmlI CAC'GTG
Cuts at: 2906 2926 2906
Size: 20 7037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5126 5158 6639 6671 5126
Size: 32 1481 32 5512
Fragments arranged by size:
5512 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2634 2940 2634
Size: 306 6751
PshAI GACnn'nnGTC
Cuts at: 1613 3398 1613
Size: 1785 5272
PsiI TTA'TAA
Cuts at: 4627 4627
Size: 7057
PspOMI G'GGCC_C
Cuts at: 2025 2025
Size: 7057
PstI C_TGCA'G
Cuts at: 830 2352 3852 3929 3998 4409 830
Size: 1522 1500 77 69 411 3478
Fragments arranged by size:
3478 1522 1500 411 77 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7057
PvuII CAG'CTG
Cuts at: 2890 3299 3961 4330 2890
Size: 409 662 369 5617
Fragments arranged by size:
5617 662 409 369
SacII CC_GC'GG
Cuts at: 3270 3270
Size: 7057
SalI G'TCGA_C
Cuts at: 1936 2597 4399 1936
Size: 661 1802 4594
Fragments arranged by size:
4594 1802 661
SanDI GG'GwC_CC
Cuts at: 2940 2940
Size: 7057
SapI GCTCTTCn'nnn_
Cuts at: 3819 3819
Size: 7057
SbfI CC_TGCA'GG
Cuts at: 4409 4409
Size: 7057
ScaI AGT'ACT
Cuts at: 3464 5305 7017 3464
Size: 1841 1712 3504
Fragments arranged by size:
3504 1841 1712
SfcI C'TryA_G
Cuts at: 826 1045 2348 3848 3925 3994 4405 5540 6221
Size: 219 1303 1500 77 69 411 1135 681
Cuts at: 6221 6412 826
Size: 191 1471
Fragments arranged by size:
1500 1471 1303 1135 681 411 219 191
77 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 2078 2078
Size: 7057
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7057
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7057
SmaI CCC'GGG
Cuts at: 1872 2024 3456 3569 4118 4384 1872
Size: 152 1432 113 549 266 4545
Fragments arranged by size:
4545 1432 549 266 152 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2112 4106 4259 5161 6062 6324
Size: 197 926 169 1994 153 902 901 262
Cuts at: 6324 820
Size: 1553
Fragments arranged by size:
1994 1553 926 902 901 262 197 169
153
SnaBI TAC'GTA
Cuts at: 493 4848 493
Size: 4355 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7057
SphI G_CATG'C
Cuts at: 1132 4415 1132
Size: 3283 3774
SspI AAT'ATT
Cuts at: 5 52 2806 4981 5
Size: 47 2754 2175 2081
Fragments arranged by size:
2754 2175 2081 47
StuI AGG'CCT
Cuts at: 1517 2303 3536 3903 1517
Size: 786 1233 367 4671
Fragments arranged by size:
4671 1233 786 367
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2169 2691 3366 3418 3597
Size: 552 939 27 138 522 675 52 179
Cuts at: 3597 3750 4496 513
Size: 153 746 3074
Fragments arranged by size:
3074 939 746 675 552 522 179 153
138 52 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2701 5084 5101 5254 5439 5884 325
Size: 1255 1121 2383 17 153 185 445 1498
Fragments arranged by size:
2383 1498 1255 1121 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3462 4293
Size: 274 80 33 51 1443 198 1287 831
Cuts at: 4293 5303 7015 96
Size: 1010 1712 138
Fragments arranged by size:
1712 1443 1287 1010 831 274 198 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 7017 7049 2744
Size: 32 4241 32 2752
Fragments arranged by size:
4241 2752 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7057
XbaI T'CTAG_A
Cuts at: 4393 4393
Size: 7057
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2742 4159 2742
Size: 1417 5640
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7057
XmaI C'CCGG_G
Cuts at: 1870 2022 3454 3567 4116 4382 1870
Size: 152 1432 113 549 266 4545
Fragments arranged by size:
4545 1432 549 266 152 113
XmnI GAAnn'nnTTC
Cuts at: 5186 5186
Size: 7057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4865 276
Size: 53 83 186 936 3331 2468
Fragments arranged by size:
3331 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI KasI
NarI NruI NsiI PacI PspXI PsrI RsrII SacI
SexAI SfoI SrfI SwaI