Restriction map for
pFN21AB5104
(Circular) (Six-base) MAPSORT of: pFN21AB5104.seq Check: 8883 from: 1 to: 6349
pFN21AB5104 6349 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2130 2183 2276 4412 5796 6064 1632
Size: 408 90 53 93 2136 1384 268 1917
Fragments arranged by size:
2136 1917 1384 408 268 93 90 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 2669 5248 6225 1203
Size: 1466 2579 977 1327
Fragments arranged by size:
2579 1466 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 2258 1166
Size: 1092 5257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6349
AlwNI CAG_nnn'CTG
Cuts at: 2299 2302 4092 5664 2299
Size: 3 1790 1572 2984
Fragments arranged by size:
2984 1790 1572 3
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2190 2309 2426 2660 3016 3562 3658 3884
Size: 697 119 117 234 356 546 96 226
Cuts at: 3884 4036 6145 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 697 546 356 234 226 152
119 117 96
AseI AT'TA_AT
Cuts at: 160 1032 3207 4903 160
Size: 872 2175 1696 1606
Fragments arranged by size:
2175 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 3674 1870
Size: 73 1731 4545
Fragments arranged by size:
4545 1731 73
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 3361 3670 5125 618
Size: 325 217 627 317 1257 309 1455 1842
Fragments arranged by size:
1842 1455 1257 627 325 317 309 217
BanII G_rGCy'C
Cuts at: 1391 2074 3000 3668 1391
Size: 683 926 668 4072
Fragments arranged by size:
4072 926 683 668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3093 928
Size: 971 1194 4184
Fragments arranged by size:
4184 1194 971
BbvCI CC'TCA_GC
Cuts at: 2421 2421
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 2412 1548
Size: 864 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4960 136
Size: 107 122 71 631 605 3288 1525
Fragments arranged by size:
3288 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 2107 3322 4410 5566 1790
Size: 317 1215 1088 1156 2573
Fragments arranged by size:
2573 1215 1156 1088 317
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 5038 442
Size: 416 53 83 399 24 2663 958 1753
Fragments arranged by size:
2663 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3333 3575 5009 1239
Size: 2094 242 1434 2579
Fragments arranged by size:
2579 2094 1434 242
Bpu10I CC'TnA_GC
Cuts at: 2421 2421
Size: 6349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3256 4474 5339 5637 3116
Size: 140 1218 865 298 3828
Fragments arranged by size:
3828 1218 865 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5012 882
Size: 617 3513 2219
Fragments arranged by size:
3513 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4140 493
Size: 711 2936 2702
Fragments arranged by size:
2936 2702 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4156 4538 5173
Size: 53 83 186 936 2098 525 382 635
Cuts at: 5173 275
Size: 1451
Fragments arranged by size:
2098 1451 936 635 525 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3020 4782 5180 5454 5601 6012 3020
Size: 1762 398 274 147 411 3357
Fragments arranged by size:
3357 1762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6124
Fragments arranged by size:
6124 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6349
BseYI C'CCAG_C
Cuts at: 2999 5552 6048 2999
Size: 2553 496 3300
Fragments arranged by size:
3300 2553 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5776
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2074 4410 4495 5566 2074
Size: 2336 85 1071 2857
Fragments arranged by size:
2857 2336 1071 85
BsmI GAATG_Cn'
Cuts at: 2358 2458 3860 3953 2358
Size: 100 1402 93 4754
Fragments arranged by size:
4754 1402 100 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2107 3000 3322 3668 4410 4495
Size: 399 284 33 893 322 346 742 85
Cuts at: 4495 5566 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 893 742 399 346 322 284
85 33
BspEI T'CCGG_A
Cuts at: 3020 6012 3020
Size: 2992 3357
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3704 844
Size: 2860 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4844 5018 65
Size: 1183 3596 174 1396
Fragments arranged by size:
3596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 2741 794
Size: 1508 439 4402
Fragments arranged by size:
4402 1508 439
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2540 2947 3007 2540
Size: 407 60 5882
Fragments arranged by size:
5882 407 60
BstYI r'GATC_y
Cuts at: 1972 2365 2725 3679 4046 4431 4448 5900 5986
Size: 393 360 954 367 385 17 1452 86
Cuts at: 5986 5998 6344 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 954 393 385 367 360 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2940 513
Size: 552 939 936 3922
Fragments arranged by size:
3922 939 936 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 3865 4659 4679 2265
Size: 1600 794 20 3935
Fragments arranged by size:
3935 1600 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 2445 3653 2445
Size: 1208 5141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3796 4685 6314
Size: 54 1425 50 275 18 1966 889 1629
Cuts at: 6314 8
Size: 43
Fragments arranged by size:
1966 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2334 3576 4279 1353
Size: 981 1242 703 3423
Fragments arranged by size:
3423 1242 981 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2072 2072
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2130 2183 2276 3333 3575 4412
Size: 393 408 90 53 93 1057 242 837
Cuts at: 4412 5009 5796 6064 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1057 837 787 597 408 393 268
242 93 90 53
EcoO109I rG'GnC_Cy
Cuts at: 2598 2598
Size: 6349
EcoRI G'AATT_C
Cuts at: 2426 2660 3658 4036 2426
Size: 234 998 378 4739
Fragments arranged by size:
4739 998 378 234
EcoRV GAT'ATC
Cuts at: 2578 2578
Size: 6349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2115 2306 2746 2115
Size: 191 440 5718
Fragments arranged by size:
5718 440 191
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2115 2147 3362 3394 4990
Size: 32 135 32 543 32 1215 32 1596
Cuts at: 4990 5022 5064 5096 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1596 1215 543 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3693 3939 669
Size: 988 281 1320 435 246 3079
Fragments arranged by size:
3079 1320 988 435 281 246
HindIII A'AGCT_T
Cuts at: 748 3240 748
Size: 2492 3857
HpaI GTT'AAC
Cuts at: 3258 3939 3258
Size: 681 5668
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3185 3388 5463 5647 1305
Size: 1880 203 2075 184 2007
Fragments arranged by size:
2075 2007 1880 203 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4527
Fragments arranged by size:
4527 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 2258 2934 3396 4307 4666 4825
Size: 648 976 116 676 462 911 359 159
Cuts at: 4825 518
Size: 2042
Fragments arranged by size:
2042 976 911 676 648 462 359 159
116
MspA1I CmG'CkG
Cuts at: 1606 2203 2299 2954 3765 4442 5590 5835 1606
Size: 597 96 655 811 677 1148 245 2120
Fragments arranged by size:
2120 1148 811 677 655 597 245 96
NcoI C'CATG_G
Cuts at: 513 1065 2004 2940 513
Size: 552 939 936 3922
Fragments arranged by size:
3922 939 936 552
NdeI CA'TA_TG
Cuts at: 387 2139 387
Size: 1752 4597
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 2216 3707 5252 1132
Size: 97 987 1491 1545 2229
Fragments arranged by size:
2229 1545 1491 987 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2598 2598
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 2388 3919 2388
Size: 1531 4818
PstI C_TGCA'G
Cuts at: 830 3701 830
Size: 2871 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2203 2299 2954 2203
Size: 96 655 5598
Fragments arranged by size:
5598 655 96
SacI G_AGCT'C
Cuts at: 2074 2074
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 3179 3697 4832 5513 5704 826
Size: 219 2134 518 1135 681 191 1471
Fragments arranged by size:
2134 1471 1135 681 518 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3131 3271 4453 5354 5616 820
Size: 197 926 1188 140 1182 901 262 1553
Fragments arranged by size:
1553 1188 1182 926 901 262 197 140
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 2990 152
Size: 2838 3511
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 2763 1517
Size: 1246 5103
StyI C'CwwG_G
Cuts at: 513 1065 2004 2940 3788 513
Size: 552 939 936 848 3074
Fragments arranged by size:
3074 939 936 848 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3265 4376 4393 4546 4731 5176 325
Size: 1255 1685 1111 17 153 185 445 1498
Fragments arranged by size:
1685 1498 1255 1111 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4595 6307 96
Size: 274 80 33 51 1443 2618 1712 138
Fragments arranged by size:
2618 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2661 2693 6309 6341 2661
Size: 32 3616 32 2669
Fragments arranged by size:
3616 2669 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3355 3355
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 2634 2660 3537 4478 2634
Size: 26 877 941 4505
Fragments arranged by size:
4505 941 877 26
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI