Restriction map for
pFN21AB5101
(Circular) (Six-base) MAPSORT of: pFN21AB5101.seq Check: 5401 from: 1 to: 7852
pFN21AB5101 7852 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2537 2537
Size: 7852
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3009 5662 278
Size: 53 83 186 936 1473 2653 2468
Fragments arranged by size:
2653 2468 1473 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2292 3099 3105 3462 5047 5195 1937
Size: 355 807 6 357 1585 148 4594
Fragments arranged by size:
4594 1585 807 357 355 148 6
Acc65I G'GTAC_C
Cuts at: 5173 5173
Size: 7852
AclI AA'CG_TT
Cuts at: 1563 5979 6352 1563
Size: 4416 373 3063
Fragments arranged by size:
4416 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2614 3626 4916 5915 7299 7567 1632
Size: 982 1012 1290 999 1384 268 1917
Fragments arranged by size:
1917 1384 1290 1012 999 982 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7655
AflIII A'CryG_T
Cuts at: 1203 3607 6751 7728 1203
Size: 2404 3144 977 1327
Fragments arranged by size:
3144 2404 1327 977
AgeI A'CCGG_T
Cuts at: 6683 6683
Size: 7852
AhdI GACnn_n'nnGTC
Cuts at: 4478 6581 4478
Size: 2103 5749
AleI CACnn'nnGTG
Cuts at: 1166 4958 1166
Size: 3792 4060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2301 1184
Size: 1117 6735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4379 4411 4379
Size: 32 7820
AlwNI CAG_nnn'CTG
Cuts at: 2704 3331 3340 3724 4106 4561 4729 5595 7167
Size: 627 9 384 382 455 168 866 1572
Cuts at: 7167 2704
Size: 3389
Fragments arranged by size:
3389 1572 866 627 455 384 382 168
9
ApaI G_GGCC'C
Cuts at: 2060 2169 3299 4779 2060
Size: 109 1130 1480 5133
Fragments arranged by size:
5133 1480 1130 109
ApaLI G'TGCA_C
Cuts at: 2326 5909 7065 2326
Size: 3583 1156 3113
Fragments arranged by size:
3583 3113 1156
ApoI r'AATT_y
Cuts at: 1493 2339 2559 2843 3791 5161 5387 5539 7648
Size: 846 220 284 948 1370 226 152 2109
Cuts at: 7648 1493
Size: 1697
Fragments arranged by size:
2109 1697 1370 948 846 284 226 220
152
AseI AT'TA_AT
Cuts at: 160 1032 6406 160
Size: 872 5374 1606
Fragments arranged by size:
5374 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7852
AvaI C'yCGr_G
Cuts at: 1870 1943 2161 2170 2744 3744 4440 4655 4714
Size: 73 218 9 574 1000 696 215 59
Cuts at: 4714 4772 5177 1870
Size: 58 405 4545
Fragments arranged by size:
4545 1000 696 574 405 218 215 73
59 58 9
AvrII C'CTAG_G
Cuts at: 2818 5041 2818
Size: 2223 5629
BamHI G'GATC_C
Cuts at: 4651 5182 5549 4651
Size: 531 367 6954
Fragments arranged by size:
6954 531 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2064 2309 2525 3043 4064
Size: 325 217 627 277 245 216 518 1021
Cuts at: 4064 5102 5173 6628 618
Size: 1038 71 1455 1842
Fragments arranged by size:
1842 1455 1038 1021 627 518 325 277
245 217 216 71
BanII G_rGCy'C
Cuts at: 1391 2060 2169 2177 2449 3135 3247 3299 3314
Size: 669 109 8 272 686 112 52 15
Cuts at: 3314 3745 4500 4773 4779 5171 1391
Size: 431 755 273 6 392 4072
Fragments arranged by size:
4072 755 686 669 431 392 273 272
112 109 52 15 8 6
BbeI G_GCGC'C
Cuts at: 2068 4068 2068
Size: 2000 5852
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2871 3951 928
Size: 971 972 1080 4829
Fragments arranged by size:
4829 1080 972 971
BbvCI CC'TCA_GC
Cuts at: 4307 4307
Size: 7852
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2142 2176 3735 3769 6043 6077 1903
Size: 34 205 34 1559 34 2274 34 3678
Fragments arranged by size:
3678 2274 1559 205 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2816 5744 6954 2816
Size: 2928 1210 3714
Fragments arranged by size:
3714 2928 1210
BclI T'GATC_A
Cuts at: 1548 2754 1548
Size: 1206 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2625 3180 3242
Size: 107 122 71 631 605 953 555 62
Cuts at: 3242 4073 6463 136
Size: 831 2390 1525
Fragments arranged by size:
2390 1525 953 831 631 605 555 122
107 71 62
BglII A'GATC_T
Cuts at: 3768 3924 7847 3768
Size: 156 3923 3773
Fragments arranged by size:
3923 3773 156
BlpI GC'TnA_GC
Cuts at: 4077 5121 5269 4077
Size: 1044 148 6660
Fragments arranged by size:
6660 1044 148
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2169 2330 2528 2886 3046 3299 3685
Size: 270 109 161 198 358 160 253 386
Cuts at: 3685 4779 5913 7069 1790
Size: 1094 1134 1156 2573
Fragments arranged by size:
2573 1156 1134 1094 386 358 270 253
198 161 160 109
BmgBI CAC'GTC
Cuts at: 1318 4339 1318
Size: 3021 4831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3848 4415 5583
Size: 416 53 83 399 24 2431 567 1168
Cuts at: 5583 6541 442
Size: 958 1753
Fragments arranged by size:
2431 1753 1168 958 567 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7852
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3821
Size: 7852
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2585 3682 4083 4129 4664 5118 6512
Size: 1148 198 1097 401 46 535 454 1394
Cuts at: 6512 1239
Size: 2579
Fragments arranged by size:
2579 1394 1148 1097 535 454 401 198
46
Bpu10I CC'TnA_GC
Cuts at: 3578 4151 4307 4368 3578
Size: 573 156 61 7062
Fragments arranged by size:
7062 573 156 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5111 5977 6842 7140 5111
Size: 866 865 298 5823
Fragments arranged by size:
5823 866 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3254 3390 4437 6515 882
Size: 617 1755 136 1047 2078 2219
Fragments arranged by size:
2219 2078 1755 1047 617 136
BsaAI yAC'GTr
Cuts at: 493 1204 5643 493
Size: 711 4439 2702
Fragments arranged by size:
4439 2702 711
BsaBI GATnn'nnATC
Cuts at: 3767 3818 5009 7846 3767
Size: 51 1191 2837 3773
Fragments arranged by size:
3773 2837 1191 51
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2065 3006 4065
Size: 53 83 186 936 485 47 941 1059
Cuts at: 4065 4992 5659 6041 6676 275
Size: 927 667 382 635 1451
Fragments arranged by size:
1451 1059 941 936 927 667 635 485
382 186 83 53 47
BsaWI w'CCGG_w
Cuts at: 6285 6683 6957 7104 7515 6285
Size: 398 274 147 411 6622
Fragments arranged by size:
6622 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 4081 4111 4379
Size: 30 165 30 996 30 1652 30 268
Cuts at: 4379 4409 4733 4763 5076 5106 1178
Size: 30 324 30 313 30 3924
Fragments arranged by size:
3924 1652 996 324 313 268 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 2446 3402 3545 4475 1186
Size: 1225 35 956 143 930 4563
Fragments arranged by size:
4563 1225 956 930 143 35
BseYI C'CCAG_C
Cuts at: 2052 2149 2595 3118 3264 3307 4514 7055 7551
Size: 97 446 523 146 43 1207 2541 496
Cuts at: 7551 2052
Size: 2353
Fragments arranged by size:
2541 2353 1207 523 496 446 146 97
43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 4594 4652 4766 1163
Size: 573 327 2531 58 114 4249
Fragments arranged by size:
4249 2531 573 327 114 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2210 5302 6063 7091 1540
Size: 275 186 209 3092 761 1028 2301
Fragments arranged by size:
3092 2301 1028 761 275 209 186
BsiHKAI G_wGCw'C
Cuts at: 2245 2330 4500 4773 4854 5913 5998 7069 2245
Size: 85 2170 273 81 1059 85 1071 3028
Fragments arranged by size:
3028 2170 1071 1059 273 85 85 81
BsmI GAATG_Cn'
Cuts at: 4024 5363 5456 4024
Size: 1339 93 6420
Fragments arranged by size:
6420 1339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2169 2177 2245 2330 2449 2528
Size: 399 270 109 8 68 85 119 79
Cuts at: 2528 2886 3046 3135 3247 3299 3314 3685 3745
Size: 358 160 89 112 52 15 371 60
Cuts at: 3745 4500 4773 4779 4854 5171 5913 5998 7069
Size: 755 273 6 75 317 742 85 1071
Cuts at: 7069 1391
Size: 2174
Fragments arranged by size:
2174 1071 755 742 399 371 358 317
273 270 160 119 112 109 89 85
85 79 75 68 60 52 15 8
6
BspEI T'CCGG_A
Cuts at: 7515 7515
Size: 7852
BspHI T'CATG_A
Cuts at: 5741 7471 5741
Size: 1730 6122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2537 3117 3975 4483 5207 844
Size: 1693 580 858 508 724 3489
Fragments arranged by size:
3489 1693 858 724 580 508
BsrBI CCG'CTC
Cuts at: 1394 1823 2583 3535 5739 1394
Size: 429 760 952 2204 3507
Fragments arranged by size:
3507 2204 952 760 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3415 4970 6347 6521 65
Size: 1183 2167 1555 1377 174 1396
Fragments arranged by size:
2167 1555 1396 1377 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 3122 6496 6683 1700
Size: 110 395 917 3374 187 2869
Fragments arranged by size:
3374 2869 917 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 2481 4884 96
Size: 2385 2403 3064
Fragments arranged by size:
3064 2403 2385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7852
BssSI C'ACGA_G
Cuts at: 5912 6924 5912
Size: 1012 6840
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7852
BstBI TT'CG_AA
Cuts at: 6656 6656
Size: 7852
BstEII G'GTnAC_C
Cuts at: 1184 4087 4378 1184
Size: 2903 291 4658
Fragments arranged by size:
4658 2903 291
BstYI r'GATC_y
Cuts at: 1972 2700 3768 3924 4302 4651 4725 5182 5549
Size: 728 1068 156 378 349 74 457 367
Cuts at: 5549 5934 5951 7403 7489 7501 7847 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1068 728 457 385 378 367
349 346 156 86 74 17 12
BstZ17I GTA'TAC
Cuts at: 3463 3463
Size: 7852
Bsu36I CC'TnA_GG
Cuts at: 3081 3776 3872 4404 3081
Size: 695 96 532 6529
Fragments arranged by size:
6529 695 532 96
BtgI C'CryG_G
Cuts at: 513 1065 2004 2449 2520 3865 4359 5109 513
Size: 552 939 445 71 1345 494 750 3256
Fragments arranged by size:
3256 1345 939 750 552 494 445 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3805 487
Size: 683 56 2579 4534
Fragments arranged by size:
4534 2579 683 56
BtsI GCAGTG_nn'
Cuts at: 2429 2997 4416 4877 5368 6162 6182 2429
Size: 568 1419 461 491 794 20 4099
Fragments arranged by size:
4099 1419 794 568 491 461 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7852
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7817
DraI TTT'AAA
Cuts at: 5156 5156
Size: 7852
DraIII CAC_nnn'GTG
Cuts at: 2041 2325 2041
Size: 284 7568
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2023 6859 809
Size: 507 707 4836 1802
Fragments arranged by size:
4836 1802 707 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2653 2994
Size: 54 1425 50 275 18 377 446 341
Cuts at: 2994 3157 3172 3234 4950 5299 6188 7817 8
Size: 163 15 62 1716 349 889 1629 43
Fragments arranged by size:
1716 1629 1425 889 446 377 349 341
275 163 62 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2207 5299 1537
Size: 275 395 3092 4090
Fragments arranged by size:
4090 3092 395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5782 1353
Size: 4429 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6439 6813 6959 387
Size: 1064 138 4850 374 146 1280
Fragments arranged by size:
4850 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4498 4771 4498
Size: 273 7579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2585 2614 3626 3682 4083 4129
Size: 393 755 198 29 1012 56 401 46
Cuts at: 4129 4664 4916 5118 5915 6512 7299 7567 1239
Size: 535 252 202 797 597 787 268 1524
Fragments arranged by size:
1524 1012 797 787 755 597 535 401
393 268 252 202 198 56 46 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 3212 2765
Size: 447 7405
EcoO109I rG'GnC_Cy
Cuts at: 2056 2057 2146 2165 2166 3205 3295 3435 4465
Size: 1 89 19 1 1039 90 140 1030
Cuts at: 4465 4472 4511 4639 4718 4776 2056
Size: 7 39 128 79 58 5132
Fragments arranged by size:
5132 1039 1030 140 128 90 89 79
58 39 19 7 1 1
EcoRI G'AATT_C
Cuts at: 2559 2843 5161 5539 2559
Size: 284 2318 378 4872
Fragments arranged by size:
4872 2318 378 284
EcoRV GAT'ATC
Cuts at: 3416 3416
Size: 7852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4855 4855
Size: 7852
FspI TGC'GCA
Cuts at: 6358 6358
Size: 7852
HaeII r_GCGC'y
Cuts at: 1678 2068 4068 6999 1678
Size: 390 2000 2931 2531
Fragments arranged by size:
2931 2531 2000 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 2924 2956 3791
Size: 32 135 32 827 32 493 32 835
Cuts at: 3791 3823 4464 4496 4710 4742 4786 4818 6493
Size: 32 641 32 214 32 44 32 1675
Cuts at: 6493 6525 6567 6599 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1675 835 827 641 493 214 135
44 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5196 5442 669
Size: 988 281 3258 246 3079
Fragments arranged by size:
3258 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5069 748
Size: 4321 3531
HpaI GTT'AAC
Cuts at: 5442 5442
Size: 7852
KasI G'GCGC_C
Cuts at: 2064 4064 2064
Size: 2000 5852
KpnI G_GTAC'C
Cuts at: 5177 5177
Size: 7852
MfeI C'AATT_G
Cuts at: 5451 5451
Size: 7852
MluI A'CGCG_T
Cuts at: 7728 7728
Size: 7852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 6966 7150 1305
Size: 3324 2337 184 2007
Fragments arranged by size:
3324 2337 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3159 3236 4952 10
Size: 54 1425 343 1327 77 1716 2910
Fragments arranged by size:
2910 1716 1425 1327 343 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3956 4491 4958 5108 5810 6169 6328
Size: 648 2790 535 467 150 702 359 159
Cuts at: 6328 518
Size: 2042
Fragments arranged by size:
2790 2042 702 648 535 467 359 159
150
MspA1I CmG'CkG
Cuts at: 1606 2047 2077 2086 2487 2595 2776 3646 3973
Size: 441 30 9 401 108 181 870 327
Cuts at: 3973 4548 4846 4927 4958 5268 5945 7093 7338
Size: 575 298 81 31 310 677 1148 245
Cuts at: 7338 1606
Size: 2120
Fragments arranged by size:
2120 1148 870 677 575 441 401 327
310 298 245 181 108 81 31 30
9
NaeI GCC'GGC
Cuts at: 3124 3124
Size: 7852
NarI GG'CG_CC
Cuts at: 2065 4065 2065
Size: 2000 5852
NcoI C'CATG_G
Cuts at: 513 1065 2004 2449 5109 513
Size: 552 939 445 2660 3256
Fragments arranged by size:
3256 2660 939 552 445
NdeI CA'TA_TG
Cuts at: 387 5098 387
Size: 4711 3141
NgoMIV G'CCGG_C
Cuts at: 3122 3122
Size: 7852
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7852
NotI GC'GGCC_GC
Cuts at: 5299 5299
Size: 7852
NspI r_CATG'y
Cuts at: 1132 1229 2472 5210 6755 1132
Size: 97 1243 2738 1545 2229
Fragments arranged by size:
2738 2229 1545 1243 97
PasI CC'CwG_GG
Cuts at: 1348 2115 3199 4133 1348
Size: 767 1084 934 5067
Fragments arranged by size:
5067 1084 934 767
PciI A'CATG_T
Cuts at: 6751 6751
Size: 7852
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4051 4233 4561 1108
Size: 2943 182 328 4399
Fragments arranged by size:
4399 2943 328 182
PfoI T'CCnGG_A
Cuts at: 2364 4266 2364
Size: 1902 5950
PmeI GTTT'AAAC
Cuts at: 5156 5156
Size: 7852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 5921 5953 7434 7466 4379
Size: 32 1510 32 1481 32 4765
Fragments arranged by size:
4765 1510 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3205 3435 4465 4472 4718 3205
Size: 230 1030 7 246 6339
Fragments arranged by size:
6339 1030 246 230 7
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7852
PsiI TTA'TAA
Cuts at: 5422 5422
Size: 7852
PspOMI G'GGCC_C
Cuts at: 2056 2165 3295 4775 2056
Size: 109 1130 1480 5133
Fragments arranged by size:
5133 1480 1130 109
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2885 2917 3986 4018 2885
Size: 32 1069 32 6719
Fragments arranged by size:
6719 1069 32 32
PstI C_TGCA'G
Cuts at: 830 2280 2781 3114 3336 3545 3972 4809 4872
Size: 1450 501 333 222 209 427 837 63
Cuts at: 4872 5204 830
Size: 332 3478
Fragments arranged by size:
3478 1450 837 501 427 333 332 222
209 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7852
PvuII CAG'CTG
Cuts at: 2047 2487 2595 2776 3646 3973 4548 4927 4958
Size: 440 108 181 870 327 575 379 31
Cuts at: 4958 2047
Size: 4941
Fragments arranged by size:
4941 870 575 440 379 327 181 108
31
SacI G_AGCT'C
Cuts at: 4500 4773 4500
Size: 273 7579
SalI G'TCGA_C
Cuts at: 1936 5194 1936
Size: 3258 4594
SbfI CC_TGCA'GG
Cuts at: 5204 5204
Size: 7852
ScaI AGT'ACT
Cuts at: 2157 4112 6100 7812 2157
Size: 1955 1988 1712 2197
Fragments arranged by size:
2197 1988 1955 1712
SexAI A'CCwGG_T
Cuts at: 2760 2760
Size: 7852
SfcI C'TryA_G
Cuts at: 826 1045 2276 2777 3110 3332 3541 3968 4805
Size: 219 1231 501 333 222 209 427 837
Cuts at: 4805 4868 5200 6335 7016 7207 826
Size: 63 332 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 837 681 501 427 333
332 222 219 209 191 63
SfoI GGC'GCC
Cuts at: 2066 4066 2066
Size: 2000 5852
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7852
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7852
SmaI CCC'GGG
Cuts at: 1872 2172 5179 1872
Size: 300 3007 4545
Fragments arranged by size:
4545 3007 300
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3744 5126 5956 6857 7119 820
Size: 197 926 1801 1382 830 901 262 1553
Fragments arranged by size:
1801 1553 1382 926 901 830 262 197
SnaBI TAC'GTA
Cuts at: 493 5643 493
Size: 5150 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7852
SphI G_CATG'C
Cuts at: 1132 2472 5210 1132
Size: 1340 2738 3774
Fragments arranged by size:
3774 2738 1340
SspI AAT'ATT
Cuts at: 5 52 5776 5
Size: 47 5724 2081
Fragments arranged by size:
5724 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7852
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2303 2449 2620 2818 3837
Size: 552 939 243 56 146 171 198 1019
Cuts at: 3837 4507 4971 5041 5109 5291 513
Size: 670 464 70 68 182 3074
Fragments arranged by size:
3074 1019 939 670 552 464 243 198
182 171 146 70 68 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 2260 3132 3998 4043 4146 5879
Size: 1255 548 132 872 866 45 103 1733
Cuts at: 5879 5896 6049 6234 6679 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1733 1498 1255 872 866 548 445 185
153 132 103 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 2409 2481
Size: 274 80 33 51 1443 178 254 72
Cuts at: 2481 4110 4884 6098 7810 96
Size: 1629 774 1214 1712 138
Fragments arranged by size:
1712 1629 1443 1214 774 274 254 178
138 80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2814 2846 4081 4113 4733 4765 7812
Size: 32 604 32 1235 32 620 32 3047
Cuts at: 7812 7844 2178
Size: 32 2186
Fragments arranged by size:
3047 2186 1235 620 604 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2222 1316
Size: 906 6946
XbaI T'CTAG_A
Cuts at: 5188 5188
Size: 7852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2484 3167 4428 2484
Size: 683 1261 5908
Fragments arranged by size:
5908 1261 683
XhoI C'TCGA_G
Cuts at: 1943 3744 1943
Size: 1801 6051
XmaI C'CCGG_G
Cuts at: 1870 2170 5177 1870
Size: 300 3007 4545
Fragments arranged by size:
4545 3007 300
XmnI GAAnn'nnTTC
Cuts at: 5981 5981
Size: 7852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3007 5660 276
Size: 53 83 186 936 1473 2653 2468
Fragments arranged by size:
2653 2468 1473 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BsmBI BstXI FseI
FspAI NruI NsiI PacI PmlI PspXI RsrII SacII
SanDI SapI SfiI SrfI SwaI