Restriction map for
pFN21AB5099
(Circular) (Six-base) MAPSORT of: pFN21AB5099.seq Check: 6939 from: 1 to: 7015
pFN21AB5099 7015 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4825 278
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4358 1937
Size: 2421 4594
Acc65I G'GTAC_C
Cuts at: 4336 4336
Size: 7015
AclI AA'CG_TT
Cuts at: 1563 4314 5142 5515 1563
Size: 2751 828 373 3063
Fragments arranged by size:
3063 2751 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 3429 3678 3686 4286 5078 6462 6730
Size: 1013 784 249 8 600 792 1384 268
Cuts at: 6730 1632
Size: 1917
Fragments arranged by size:
1917 1384 1013 792 784 600 268 249
8
AflII C'TTAA_G
Cuts at: 820 1017 3074 4196 820
Size: 197 2057 1122 3639
Fragments arranged by size:
3639 2057 1122 197
AflIII A'CryG_T
Cuts at: 1203 5914 6891 1203
Size: 4711 977 1327
Fragments arranged by size:
4711 1327 977
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 5744 5744
Size: 7015
AleI CACnn'nnGTG
Cuts at: 1166 2433 1166
Size: 1267 5748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7015
AlwNI CAG_nnn'CTG
Cuts at: 4758 6330 4758
Size: 1572 5443
ApaI G_GGCC'C
Cuts at: 4207 4207
Size: 7015
ApaLI G'TGCA_C
Cuts at: 5072 6228 5072
Size: 1156 5859
ApoI r'AATT_y
Cuts at: 1493 2180 2419 2645 2665 3010 3224 3546 4324
Size: 687 239 226 20 345 214 322 778
Cuts at: 4324 4550 4702 6811 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 778 687 345 322 239 226
226 214 152 20
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 4340 1870
Size: 73 2397 4545
Fragments arranged by size:
4545 2397 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2596 2629 2596
Size: 33 6982
BamHI G'GATC_C
Cuts at: 4345 4712 4345
Size: 367 6648
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4336 5791 618
Size: 325 217 627 2549 1455 1842
Fragments arranged by size:
2549 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4207 4334 1391
Size: 2816 127 4072
Fragments arranged by size:
4072 2816 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5206 5240 1903
Size: 34 3269 34 3678
Fragments arranged by size:
3678 3269 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3383 4907 6117 3383
Size: 1524 1210 4281
Fragments arranged by size:
4281 1524 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7015
BfrBI ATG'CAT
Cuts at: 2189 3784 2189
Size: 1595 5420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5626 136
Size: 107 122 71 631 605 3954 1525
Fragments arranged by size:
3954 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7010 7010
Size: 7015
BlpI GC'TnA_GC
Cuts at: 4432 4432
Size: 7015
Bme1580I G_kGCm'C
Cuts at: 1790 4207 5076 6232 1790
Size: 2417 869 1156 2573
Fragments arranged by size:
2573 2417 1156 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 4746 5704
Size: 416 53 83 399 24 728 2601 958
Cuts at: 5704 442
Size: 1753
Fragments arranged by size:
2601 1753 958 728 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2042 1056
Size: 986 6029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2427 5675 1239
Size: 1188 3248 2579
Fragments arranged by size:
3248 2579 1188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2325 3491 5140 6005 6303 2325
Size: 1166 1649 865 298 3037
Fragments arranged by size:
3037 1649 1166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5678 882
Size: 617 4179 2219
Fragments arranged by size:
4179 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4806 493
Size: 711 3602 2702
Fragments arranged by size:
3602 2702 711
BsaBI GATnn'nnATC
Cuts at: 3858 7009 3858
Size: 3151 3864
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4822 5204 5839 275
Size: 53 83 186 936 3289 382 635 1451
Fragments arranged by size:
3289 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5448 5846 6120 6267 6678 5448
Size: 398 274 147 411 5785
Fragments arranged by size:
5785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6790
Fragments arranged by size:
6790 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3603 1186
Size: 2417 4598
BseYI C'CCAG_C
Cuts at: 3725 4158 6218 6714 3725
Size: 433 2060 496 4026
Fragments arranged by size:
4026 2060 496 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2035 2392 1163
Size: 573 299 357 5786
Fragments arranged by size:
5786 573 357 299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4465 5226 6254 1540
Size: 275 186 2464 761 1028 2301
Fragments arranged by size:
2464 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 4136 5076 5161 6232 2268
Size: 1868 940 85 1071 3051
Fragments arranged by size:
3051 1868 1071 940 85
BsmI GAATG_Cn'
Cuts at: 2329 4237 4526 4619 2329
Size: 1908 289 93 4725
Fragments arranged by size:
4725 1908 289 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 4136 4207 4334 5076 5161 6232
Size: 399 478 1868 71 127 742 85 1071
Cuts at: 6232 1391
Size: 2174
Fragments arranged by size:
2174 1868 1071 742 478 399 127 85
71
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 2556 2634 3850 4904 6634 2556
Size: 78 1216 1054 1730 2937
Fragments arranged by size:
2937 1730 1216 1054 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2880 2920 3703 4058 4153 4370 844
Size: 2036 40 783 355 95 217 3489
Fragments arranged by size:
3489 2036 783 355 217 95 40
BsrBI CCG'CTC
Cuts at: 1394 1823 4902 1394
Size: 429 3079 3507
Fragments arranged by size:
3507 3079 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3544 3842 3902 4239 5510 5684 65
Size: 1183 2296 298 60 337 1271 174 1396
Fragments arranged by size:
2296 1396 1271 1183 337 298 174 60
BsrFI r'CCGG_y
Cuts at: 1700 1810 2574 5659 5846 1700
Size: 110 764 3085 187 2869
Fragments arranged by size:
3085 2869 764 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 5075 6087 5075
Size: 1012 6003
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7015
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7015
BstYI r'GATC_y
Cuts at: 1972 2146 3859 4345 4712 5097 5114 6566 6652
Size: 174 1713 486 367 385 17 1452 86
Cuts at: 6652 6664 7010 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1713 1452 486 385 367 346 174
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4043 4043
Size: 7015
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6276
Fragments arranged by size:
6276 683 56
BtsI GCAGTG_nn'
Cuts at: 2268 2700 2784 4531 5325 5345 2268
Size: 432 84 1747 794 20 3938
Fragments arranged by size:
3938 1747 794 432 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 3328 3552 4319 3328
Size: 224 767 6024
Fragments arranged by size:
6024 767 224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6022 809
Size: 507 4706 1802
Fragments arranged by size:
4706 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4462 5351 6980
Size: 54 1425 50 275 18 2632 889 1629
Cuts at: 6980 8
Size: 43
Fragments arranged by size:
2632 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4462 1537
Size: 275 2650 4090
Fragments arranged by size:
4090 2650 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4945 1353
Size: 3592 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3182 5602 5976 6122 387
Size: 1064 138 1593 2420 374 146 1280
Fragments arranged by size:
2420 1593 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2427 2645 3429 3678 3686 4286 5078
Size: 393 795 218 784 249 8 600 792
Cuts at: 5078 5675 6462 6730 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 795 792 787 784 600 597 393
268 249 218 8
EcoO109I rG'GnC_Cy
Cuts at: 4047 4081 4203 4204 4047
Size: 34 122 1 6858
Fragments arranged by size:
6858 122 34 1
EcoRI G'AATT_C
Cuts at: 2419 4324 4702 2419
Size: 1905 378 4732
Fragments arranged by size:
4732 1905 378
EcoRV GAT'ATC
Cuts at: 2632 2632
Size: 7015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 7015
FspI TGC'GCA
Cuts at: 4054 5521 4054
Size: 1467 5548
HaeII r_GCGC'y
Cuts at: 1678 6162 1678
Size: 4484 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2964 2996 5656 5688 5730
Size: 32 135 32 1392 32 2660 32 42
Cuts at: 5730 5762 1373
Size: 32 2626
Fragments arranged by size:
2660 2626 1392 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4359 4605 669
Size: 988 281 2421 246 3079
Fragments arranged by size:
3079 2421 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2397 2748 748
Size: 1649 351 5015
Fragments arranged by size:
5015 1649 351
HpaI GTT'AAC
Cuts at: 4605 4605
Size: 7015
KpnI G_GTAC'C
Cuts at: 4340 4340
Size: 7015
MfeI C'AATT_G
Cuts at: 3570 4614 3570
Size: 1044 5971
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3774 3887 6129 6313 1305
Size: 2469 113 2242 184 2007
Fragments arranged by size:
2469 2242 2007 184 113
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5193
Fragments arranged by size:
5193 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2433 2772 4973 5332 5491 518
Size: 648 1267 339 2201 359 159 2042
Fragments arranged by size:
2201 2042 1267 648 359 339 159
MspA1I CmG'CkG
Cuts at: 1606 3818 4431 5108 6256 6501 1606
Size: 2212 613 677 1148 245 2120
Fragments arranged by size:
2212 2120 1148 677 613 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6463
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7015
NheI G'CTAG_C
Cuts at: 1052 2038 1052
Size: 986 6029
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NsiI A_TGCA'T
Cuts at: 2191 3786 2191
Size: 1595 5420
NspI r_CATG'y
Cuts at: 1132 1229 4373 5918 1132
Size: 97 3144 1545 2229
Fragments arranged by size:
3144 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7015
PciI A'CATG_T
Cuts at: 5914 5914
Size: 7015
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7015
PfoI T'CCnGG_A
Cuts at: 2443 2443
Size: 7015
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 6597 6629 5084
Size: 32 1481 32 5470
Fragments arranged by size:
5470 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4047 4081 4047
Size: 34 6981
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7015
PsiI TTA'TAA
Cuts at: 3336 4585 3336
Size: 1249 5766
PspOMI G'GGCC_C
Cuts at: 4203 4203
Size: 7015
PstI C_TGCA'G
Cuts at: 830 2707 4367 830
Size: 1877 1660 3478
Fragments arranged by size:
3478 1877 1660
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 3818 3818
Size: 7015
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SbfI CC_TGCA'GG
Cuts at: 4367 4367
Size: 7015
ScaI AGT'ACT
Cuts at: 5263 6975 5263
Size: 1712 5303
SfcI C'TryA_G
Cuts at: 826 1045 2703 4363 5498 6179 6370 826
Size: 219 1658 1660 1135 681 191 1471
Fragments arranged by size:
1660 1658 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 4342 1872
Size: 2470 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2340 3074 3470 4196 5119 6020
Size: 197 926 397 734 396 726 923 901
Cuts at: 6020 6282 820
Size: 262 1553
Fragments arranged by size:
1553 926 923 901 734 726 397 396
262 197
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 4373 1132
Size: 3241 3774
SspI AAT'ATT
Cuts at: 5 52 3212 3323 4939 5
Size: 47 3160 111 1616 2081
Fragments arranged by size:
3160 2081 1616 111 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7015
StyI C'CwwG_G
Cuts at: 513 1065 2725 4454 513
Size: 552 1660 1729 3074
Fragments arranged by size:
3074 1729 1660 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5042 5059 5212 5397 5842 325
Size: 1255 3462 17 153 185 445 1498
Fragments arranged by size:
3462 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2336 3287 3943
Size: 274 80 33 51 1443 359 951 656
Cuts at: 3943 3985 5261 6973 96
Size: 42 1276 1712 138
Fragments arranged by size:
1712 1443 1276 951 656 359 274 138
80 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6975 7007 6975
Size: 32 6983
Tth111I GACn'n_nGTC
Cuts at: 1316 2894 1316
Size: 1578 5437
XbaI T'CTAG_A
Cuts at: 4351 4351
Size: 7015
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7015
XmaI C'CCGG_G
Cuts at: 1870 4340 1870
Size: 2470 4545
XmnI GAAnn'nnTTC
Cuts at: 2400 3138 3460 5144 2400
Size: 738 322 1684 4271
Fragments arranged by size:
4271 1684 738 322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4823 276
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI