Restriction map for
pFN21AB5096
(Circular) (Six-base) MAPSORT of: pFN21AB5096.seq Check: 3944 from: 1 to: 6451
pFN21AB5096 6451 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4261 278
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3794 1937
Size: 1857 4594
Acc65I G'GTAC_C
Cuts at: 3772 3772
Size: 6451
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 3497 3673 4514 5898 6166 1632
Size: 454 1411 176 841 1384 268 1917
Fragments arranged by size:
1917 1411 1384 841 454 268 176
AfeI AGC'GCT
Cuts at: 2171 2336 3020 2171
Size: 165 684 5602
Fragments arranged by size:
5602 684 165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 5350 6327 1203
Size: 4147 977 1327
Fragments arranged by size:
4147 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6451
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 6419
AlwNI CAG_nnn'CTG
Cuts at: 3475 4194 5766 3475
Size: 719 1572 4160
Fragments arranged by size:
4160 1572 719
ApaI G_GGCC'C
Cuts at: 2952 2952
Size: 6451
ApaLI G'TGCA_C
Cuts at: 2717 4508 5664 2717
Size: 1791 1156 3504
Fragments arranged by size:
3504 1791 1156
ApoI r'AATT_y
Cuts at: 1493 2806 3137 3458 3760 3986 4138 6247 1493
Size: 1313 331 321 302 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 331 321 302 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5005 160
Size: 872 3973 1606
Fragments arranged by size:
3973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 2744 3776 1870
Size: 73 201 600 1032 4545
Fragments arranged by size:
4545 1032 600 201 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2140 2173 2140
Size: 33 6418
BamHI G'GATC_C
Cuts at: 3378 3781 4148 3378
Size: 403 367 5681
Fragments arranged by size:
5681 403 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2271 2488 2776 3772
Size: 325 217 627 273 211 217 288 996
Cuts at: 3772 5227 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 996 627 325 288 273 217
217 211
BanII G_rGCy'C
Cuts at: 1391 2745 2952 3330 3770 1391
Size: 1354 207 378 440 4072
Fragments arranged by size:
4072 1354 440 378 207
BbeI G_GCGC'C
Cuts at: 2275 2275
Size: 6451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3001 3136 3415 3673 928
Size: 971 1102 135 279 258 3706
Fragments arranged by size:
3706 1102 971 279 258 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2050 2084 2560 2594 4642 4676 1903
Size: 34 113 34 476 34 2048 34 3678
Fragments arranged by size:
3678 2048 476 113 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3053 4343 5553 3053
Size: 1290 1210 3951
Fragments arranged by size:
3951 1290 1210
BclI T'GATC_A
Cuts at: 1548 3576 1548
Size: 2028 4423
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2774 5062 136
Size: 107 122 71 631 605 1102 2288 1525
Fragments arranged by size:
2288 1525 1102 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6446 6446
Size: 6451
BlpI GC'TnA_GC
Cuts at: 3868 3868
Size: 6451
Bme1580I G_kGCm'C
Cuts at: 1790 2493 2721 2952 4512 5668 1790
Size: 703 228 231 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 703 231 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2173 4182 5140
Size: 416 53 83 399 24 756 2009 958
Cuts at: 5140 442
Size: 1753
Fragments arranged by size:
2009 1753 958 756 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5111 1239
Size: 3872 2579
Bpu10I CC'TnA_GC
Cuts at: 3329 3329
Size: 6451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4576 5441 5739 4576
Size: 865 298 5288
Fragments arranged by size:
5288 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 5114 882
Size: 617 858 2757 2219
Fragments arranged by size:
2757 2219 858 617
BsaAI yAC'GTr
Cuts at: 493 1204 2156 4242 493
Size: 711 952 2086 2702
Fragments arranged by size:
2702 2086 952 711
BsaBI GATnn'nnATC
Cuts at: 6445 6445
Size: 6451
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2272 3182 3694 4258
Size: 53 83 186 936 739 910 512 564
Cuts at: 4258 4640 5275 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 910 739 635 564 512 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4884 5282 5556 5703 6114 4884
Size: 398 274 147 411 5221
Fragments arranged by size:
5221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3008 3038 1178
Size: 30 165 30 1605 30 4591
Fragments arranged by size:
4591 1605 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2649 3575 3578 3611 1186
Size: 1463 926 3 33 4026
Fragments arranged by size:
4026 1463 926 33 3
BseYI C'CCAG_C
Cuts at: 2725 3220 5654 6150 2725
Size: 495 2434 496 3026
Fragments arranged by size:
3026 2434 496 495
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5878
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3545 3901 4662 5690 1540
Size: 275 186 1544 356 761 1028 2301
Fragments arranged by size:
2301 1544 1028 761 356 275 186
BsiHKAI G_wGCw'C
Cuts at: 2721 3330 4512 4597 5668 2721
Size: 609 1182 85 1071 3504
Fragments arranged by size:
3504 1182 1071 609 85
BsmI GAATG_Cn'
Cuts at: 2749 3962 4055 2749
Size: 1213 93 5145
Fragments arranged by size:
5145 1213 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3560 3560
Size: 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2721 2745 2952 3330 3770 4512
Size: 399 703 228 24 207 378 440 742
Cuts at: 4512 4597 5668 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 440 399 378 228
207 85 24
BspEI T'CCGG_A
Cuts at: 6114 6114
Size: 6451
BspHI T'CATG_A
Cuts at: 2535 4340 6070 2535
Size: 1805 1730 2916
Fragments arranged by size:
2916 1805 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 3806 844
Size: 1301 1661 3489
Fragments arranged by size:
3489 1661 1301
BsrBI CCG'CTC
Cuts at: 1394 1823 4338 1394
Size: 429 2515 3507
Fragments arranged by size:
3507 2515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4946 5120 65
Size: 1183 3698 174 1396
Fragments arranged by size:
3698 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3540 5095 5282 1700
Size: 110 1730 1555 187 2869
Fragments arranged by size:
2869 1730 1555 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 2720 4511 5523 2720
Size: 1791 1012 3648
Fragments arranged by size:
3648 1791 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6451
BstBI TT'CG_AA
Cuts at: 2326 5255 2326
Size: 2929 3522
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6451
BstYI r'GATC_y
Cuts at: 1972 2683 3378 3781 4148 4533 4550 6002 6088
Size: 711 695 403 367 385 17 1452 86
Cuts at: 6088 6100 6446 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 711 695 403 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3509 3509
Size: 6451
BtgI C'CryG_G
Cuts at: 513 1065 2560 2979 3295 3627 3697 513
Size: 552 1495 419 316 332 70 3267
Fragments arranged by size:
3267 1495 552 419 332 316 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2182 3429 487
Size: 683 56 956 1247 3509
Fragments arranged by size:
3509 1247 956 683 56
BtsI GCAGTG_nn'
Cuts at: 2471 3111 3342 3967 4761 4781 2471
Size: 640 231 625 794 20 4141
Fragments arranged by size:
4141 794 640 625 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6416
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5458 809
Size: 507 4142 1802
Fragments arranged by size:
4142 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2766 2982
Size: 54 1425 50 275 18 363 573 216
Cuts at: 2982 3076 3542 3898 4787 6416 8
Size: 94 466 356 889 1629 43
Fragments arranged by size:
1629 1425 889 573 466 363 356 275
216 94 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3542 3898 1537
Size: 275 1730 356 4090
Fragments arranged by size:
4090 1730 356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3266 3318 4381 1353
Size: 1913 52 1063 3423
Fragments arranged by size:
3423 1913 1063 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5038 5412 5558 387
Size: 1064 138 3449 374 146 1280
Fragments arranged by size:
3449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3328 3328
Size: 6451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 3497 3673 4514 5111 5898 6166
Size: 393 454 1411 176 841 597 787 268
Cuts at: 6166 1239
Size: 1524
Fragments arranged by size:
1524 1411 841 787 597 454 393 268
176
EcoNI CCTnn'n_nnAGG
Cuts at: 3121 3121
Size: 6451
EcoO109I rG'GnC_Cy
Cuts at: 2149 2621 3091 3702 2149
Size: 472 470 611 4898
Fragments arranged by size:
4898 611 472 470
EcoRI G'AATT_C
Cuts at: 3760 4138 3760
Size: 378 6073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 6451
FseI GG_CCGG'CC
Cuts at: 3544 3544
Size: 6451
FspI TGC'GCA
Cuts at: 4957 4957
Size: 6451
HaeII r_GCGC'y
Cuts at: 1678 2173 2275 2338 3022 5598 1678
Size: 495 102 63 684 2576 2531
Fragments arranged by size:
2576 2531 684 495 102 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2872 2904 3167 3199 5092
Size: 32 135 32 1300 32 263 32 1893
Cuts at: 5092 5124 5166 5198 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1893 1300 263 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3795 4041 669
Size: 988 281 1857 246 3079
Fragments arranged by size:
3079 1857 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6451
HpaI GTT'AAC
Cuts at: 4041 4041
Size: 6451
KasI G'GCGC_C
Cuts at: 2271 2271
Size: 6451
KpnI G_GTAC'C
Cuts at: 3776 3776
Size: 6451
MfeI C'AATT_G
Cuts at: 4050 4050
Size: 6451
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5565 5749 1305
Size: 4260 184 2007
Fragments arranged by size:
4260 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2984 3078 10
Size: 54 1425 343 1152 94 3383
Fragments arranged by size:
3383 1425 1152 343 94 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 2879 3587 4409 4768 4927 518
Size: 648 1588 125 708 822 359 159 2042
Fragments arranged by size:
2042 1588 822 708 648 359 159 125
MspA1I CmG'CkG
Cuts at: 1606 2192 3867 4544 5692 5937 1606
Size: 586 1675 677 1148 245 2120
Fragments arranged by size:
2120 1675 1148 677 586 245
NaeI GCC'GGC
Cuts at: 3542 3542
Size: 6451
NarI GG'CG_CC
Cuts at: 2272 2272
Size: 6451
NcoI C'CATG_G
Cuts at: 513 1065 2560 3697 513
Size: 552 1495 1137 3267
Fragments arranged by size:
3267 1495 1137 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6451
NgoMIV G'CCGG_C
Cuts at: 3540 3540
Size: 6451
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6451
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 3809 5354 1132
Size: 97 2580 1545 2229
Fragments arranged by size:
2580 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2737 1348
Size: 1389 5062
PciI A'CATG_T
Cuts at: 5350 5350
Size: 6451
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 2794 3052 1108
Size: 1629 57 258 4507
Fragments arranged by size:
4507 1629 258 57
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 3008 3040 4520 4552 6033 6065 2852
Size: 32 124 32 1480 32 1481 32 3238
Fragments arranged by size:
3238 1481 1480 124 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2149 3702 2149
Size: 1553 4898
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6451
PsiI TTA'TAA
Cuts at: 4021 4021
Size: 6451
PspOMI G'GGCC_C
Cuts at: 2948 2948
Size: 6451
PspXI vC'TCGA_Gb
Cuts at: 2744 2744
Size: 6451
PstI C_TGCA'G
Cuts at: 830 3803 830
Size: 2973 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
SacI G_AGCT'C
Cuts at: 3330 3330
Size: 6451
SalI G'TCGA_C
Cuts at: 1936 3793 1936
Size: 1857 4594
SanDI GG'GwC_CC
Cuts at: 3702 3702
Size: 6451
SapI GCTCTTCn'nnn_
Cuts at: 3318 3318
Size: 6451
SbfI CC_TGCA'GG
Cuts at: 3803 3803
Size: 6451
ScaI AGT'ACT
Cuts at: 3308 4699 6411 3308
Size: 1391 1712 3348
Fragments arranged by size:
3348 1712 1391
SfcI C'TryA_G
Cuts at: 826 1045 2120 3799 4934 5615 5806 826
Size: 219 1075 1679 1135 681 191 1471
Fragments arranged by size:
1679 1471 1135 1075 681 219 191
SfoI GGC'GCC
Cuts at: 2273 2273
Size: 6451
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 2146 3778 1872
Size: 274 1632 4545
Fragments arranged by size:
4545 1632 274
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 4555 5456 5718 820
Size: 197 926 801 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 801 262 197
SnaBI TAC'GTA
Cuts at: 493 2156 4242 493
Size: 1663 2086 2702
Fragments arranged by size:
2702 2086 1663
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6451
SphI G_CATG'C
Cuts at: 1132 3809 1132
Size: 2677 3774
SspI AAT'ATT
Cuts at: 5 52 4375 5
Size: 47 4323 2081
Fragments arranged by size:
4323 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6451
StyI C'CwwG_G
Cuts at: 513 1065 2403 2560 2663 3697 3890 513
Size: 552 1338 157 103 1034 193 3074
Fragments arranged by size:
3074 1338 1034 552 193 157 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2657 3157 4478 4495 4648 4833 5278
Size: 1255 1077 500 1321 17 153 185 445
Cuts at: 5278 325
Size: 1498
Fragments arranged by size:
1498 1321 1255 1077 500 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3306 4697 6409
Size: 274 80 33 51 1443 1329 1391 1712
Cuts at: 6409 96
Size: 138
Fragments arranged by size:
1712 1443 1391 1329 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 6411 6443 2463
Size: 32 3916 32 2471
Fragments arranged by size:
3916 2471 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3746 1316
Size: 2430 4021
XbaI T'CTAG_A
Cuts at: 3787 3787
Size: 6451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 3053 2849
Size: 204 6247
XhoI C'TCGA_G
Cuts at: 1943 2744 1943
Size: 801 5650
XmaI C'CCGG_G
Cuts at: 1870 2144 3776 1870
Size: 274 1632 4545
Fragments arranged by size:
4545 1632 274
XmnI GAAnn'nnTTC
Cuts at: 4580 4580
Size: 6451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4259 276
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BbvCI BfrBI BplI BsiWI BstXI
BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PfoI
PmlI PsrI PvuII RsrII SacII SexAI SfiI SrfI
SwaI