Restriction map for
pFN21AB5095
(Circular) (Six-base) MAPSORT of: pFN21AB5095.seq Check: 2767 from: 1 to: 7627
pFN21AB5095 7627 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5437 278
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3525 4970 1937
Size: 1588 1445 4594
Fragments arranged by size:
4594 1588 1445
Acc65I G'GTAC_C
Cuts at: 2135 4948 2135
Size: 2813 4814
AclI AA'CG_TT
Cuts at: 1563 5754 6127 1563
Size: 4191 373 3063
Fragments arranged by size:
4191 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4008 4389 5690 7074 7342 1632
Size: 2376 381 1301 1384 268 1917
Fragments arranged by size:
2376 1917 1384 1301 381 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7430
AflIII A'CryG_T
Cuts at: 1203 2313 2570 6526 7503 1203
Size: 1110 257 3956 977 1327
Fragments arranged by size:
3956 1327 1110 977 257
AgeI A'CCGG_T
Cuts at: 6458 6458
Size: 7627
AhdI GACnn_n'nnGTC
Cuts at: 6356 6356
Size: 7627
AleI CACnn'nnGTG
Cuts at: 1166 2907 3594 3791 3908 1166
Size: 1741 687 197 117 4885
Fragments arranged by size:
4885 1741 687 197 117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2255 2715 3989 4055 1184
Size: 1071 460 1274 66 4756
Fragments arranged by size:
4756 1274 1071 460 66
AlwNI CAG_nnn'CTG
Cuts at: 3929 4570 4700 5370 6942 3929
Size: 641 130 670 1572 4614
Fragments arranged by size:
4614 1572 670 641 130
ApaLI G'TGCA_C
Cuts at: 3224 5684 6840 3224
Size: 2460 1156 4011
Fragments arranged by size:
4011 2460 1156
ApoI r'AATT_y
Cuts at: 1493 2653 3375 3383 4154 4936 5162 5314 7423
Size: 1160 722 8 771 782 226 152 2109
Cuts at: 7423 1493
Size: 1697
Fragments arranged by size:
2109 1697 1160 782 771 722 226 152
8
AseI AT'TA_AT
Cuts at: 160 1032 3848 6181 160
Size: 872 2816 2333 1606
Fragments arranged by size:
2816 2333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 4588 4712 4952 1870
Size: 73 343 2302 124 240 4545
Fragments arranged by size:
4545 2302 343 240 124 73
BamHI G'GATC_C
Cuts at: 3820 3919 4125 4957 5324 3820
Size: 99 206 832 367 6123
Fragments arranged by size:
6123 832 367 206 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 3461 4948 6403 618
Size: 325 217 627 348 1326 1487 1455 1842
Fragments arranged by size:
1842 1487 1455 1326 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 2792 4946 1391
Size: 1401 2154 4072
Fragments arranged by size:
4072 2154 1401
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3678 4014 928
Size: 971 1779 336 4541
Fragments arranged by size:
4541 1779 971 336
BbvCI CC'TCA_GC
Cuts at: 2793 2793
Size: 7627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5818 5852 1903
Size: 34 3881 34 3678
Fragments arranged by size:
3881 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2293 2738 5519 6729 2293
Size: 445 2781 1210 3191
Fragments arranged by size:
3191 2781 1210 445
BclI T'GATC_A
Cuts at: 1548 4572 1548
Size: 3024 4603
BfrBI ATG'CAT
Cuts at: 2692 3079 2692
Size: 387 7240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4800 6238 136
Size: 107 122 71 631 605 3128 1438 1525
Fragments arranged by size:
3128 1525 1438 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7622 7622
Size: 7627
BlpI GC'TnA_GC
Cuts at: 4829 5044 4829
Size: 215 7412
Bme1580I G_kGCm'C
Cuts at: 1790 3228 5688 6844 1790
Size: 1438 2460 1156 2573
Fragments arranged by size:
2573 2460 1438 1156
BmgBI CAC'GTC
Cuts at: 1318 2573 2916 3793 1318
Size: 1255 343 877 5152
Fragments arranged by size:
5152 1255 877 343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4745 5358 6316
Size: 416 53 83 399 24 3328 613 958
Cuts at: 6316 442
Size: 1753
Fragments arranged by size:
3328 1753 958 613 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7627
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2439 4053 4603 2439
Size: 1614 550 5463
Fragments arranged by size:
5463 1614 550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2991 3540 3698 3951 6287 1239
Size: 1752 549 158 253 2336 2579
Fragments arranged by size:
2579 2336 1752 549 253 158
Bpu10I CC'TnA_GC
Cuts at: 2793 2793
Size: 7627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3427 3775 5752 6617 6915 3427
Size: 348 1977 865 298 4139
Fragments arranged by size:
4139 1977 865 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6290 882
Size: 617 4791 2219
Fragments arranged by size:
4791 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5418 493
Size: 711 4214 2702
Fragments arranged by size:
4214 2702 711
BsaBI GATnn'nnATC
Cuts at: 7621 7621
Size: 7627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5434 5816 6451 275
Size: 53 83 186 936 3901 382 635 1451
Fragments arranged by size:
3901 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4630 6060 6458 6732 6879 7290 4630
Size: 1430 398 274 147 411 4967
Fragments arranged by size:
4967 1430 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2631 2661 4584 4614 1178
Size: 30 165 30 1228 30 1923 30 4191
Fragments arranged by size:
4191 1923 1228 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2481 4525 4898 4912 1186
Size: 1295 2044 373 14 3901
Fragments arranged by size:
3901 2044 1295 373 14
BseYI C'CCAG_C
Cuts at: 2632 6830 7326 2632
Size: 4198 496 2933
Fragments arranged by size:
4198 2933 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3354 3995 1163
Size: 573 1618 641 4795
Fragments arranged by size:
4795 1618 641 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5077 5838 6866 1540
Size: 275 186 3076 761 1028 2301
Fragments arranged by size:
3076 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3228 5688 5773 6844 3228
Size: 2460 85 1071 4011
Fragments arranged by size:
4011 2460 1071 85
BsmI GAATG_Cn'
Cuts at: 4174 5138 5231 4174
Size: 964 93 6570
Fragments arranged by size:
6570 964 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4709 4709
Size: 7627
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2792 3228 4946 5688 5773 6844 1391
Size: 399 1002 436 1718 742 85 1071 2174
Fragments arranged by size:
2174 1718 1071 1002 742 436 399 85
BspEI T'CCGG_A
Cuts at: 7290 7290
Size: 7627
BspHI T'CATG_A
Cuts at: 2982 4351 5516 7246 2982
Size: 1369 1165 1730 3363
Fragments arranged by size:
3363 1730 1369 1165
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4982 844
Size: 4138 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5514 1394
Size: 429 3691 3507
Fragments arranged by size:
3691 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4650 6122 6296 65
Size: 1183 3402 1472 174 1396
Fragments arranged by size:
3402 1472 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4824 6271 6458 1700
Size: 110 3014 1447 187 2869
Fragments arranged by size:
3014 2869 1447 187 110
BsrGI T'GTAC_A
Cuts at: 96 2381 4270 4383 96
Size: 2285 1889 113 3340
Fragments arranged by size:
3340 2285 1889 113
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7627
BssSI C'ACGA_G
Cuts at: 5687 6699 5687
Size: 1012 6615
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4552 794
Size: 3758 3869
BstBI TT'CG_AA
Cuts at: 6431 6431
Size: 7627
BstEII G'GTnAC_C
Cuts at: 1184 2846 3596 1184
Size: 1662 750 5215
Fragments arranged by size:
5215 1662 750
BstXI CCAn_nnnn'nTGG
Cuts at: 3548 3722 3910 3548
Size: 174 188 7265
Fragments arranged by size:
7265 188 174
BstYI r'GATC_y
Cuts at: 1972 3304 3691 3820 3919 4125 4957 5324 5709
Size: 1332 387 129 99 206 832 367 385
Cuts at: 5709 5726 7178 7264 7276 7622 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1332 832 387 385 367 346
206 129 99 86 17 12
BstZ17I GTA'TAC
Cuts at: 3526 3526
Size: 7627
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6136
Fragments arranged by size:
6136 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6888
Fragments arranged by size:
6888 683 56
BtsI GCAGTG_nn'
Cuts at: 3883 4239 4849 5143 5937 5957 3883
Size: 356 610 294 794 20 5553
Fragments arranged by size:
5553 794 610 356 294 20
ClaI AT'CG_AT
Cuts at: 1554 4447 1554
Size: 2893 4734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2887 2922 3735 3770 529
Size: 35 2323 35 813 35 4386
Fragments arranged by size:
4386 2323 813 35 35 35
DraI TTT'AAA
Cuts at: 2098 4138 4931 2098
Size: 2040 793 4794
Fragments arranged by size:
4794 2040 793
DraIII CAC_nnn'GTG
Cuts at: 3455 3989 4008 4413 3455
Size: 534 19 405 6669
Fragments arranged by size:
6669 534 405 19
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6634 809
Size: 507 5318 1802
Fragments arranged by size:
5318 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 4647 5074
Size: 54 1425 50 275 18 181 2636 427
Cuts at: 5074 5963 7592 8
Size: 889 1629 43
Fragments arranged by size:
2636 1629 1425 889 427 275 181 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5074 1537
Size: 275 3262 4090
Fragments arranged by size:
4090 3262 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3495 4011 4723 4875 5557 1353
Size: 2142 516 712 152 682 3423
Fragments arranged by size:
3423 2142 712 682 516 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6214 6588 6734 387
Size: 1064 138 4625 374 146 1280
Fragments arranged by size:
4625 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2991 3540 3698 3951 4008 4389 5690
Size: 393 1359 549 158 253 57 381 1301
Cuts at: 5690 6287 7074 7342 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1359 1301 787 597 549 393 381
268 253 158 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3456 4041 4272 3456
Size: 585 231 6811
Fragments arranged by size:
6811 585 231
EcoO109I rG'GnC_Cy
Cuts at: 3839 4683 3839
Size: 844 6783
EcoRI G'AATT_C
Cuts at: 2653 3375 4936 5314 2653
Size: 722 1561 378 4966
Fragments arranged by size:
4966 1561 722 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4441 4441
Size: 7627
FspI TGC'GCA
Cuts at: 4331 6133 4331
Size: 1802 5825
HaeII r_GCGC'y
Cuts at: 1678 6774 1678
Size: 5096 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3602 3634 6268 6300 6342
Size: 32 135 32 2030 32 2634 32 42
Cuts at: 6342 6374 1373
Size: 32 2626
Fragments arranged by size:
2634 2626 2030 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2620 3899 4971 5217 669
Size: 988 281 682 1279 1072 246 3079
Fragments arranged by size:
3079 1279 1072 988 682 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7627
HpaI GTT'AAC
Cuts at: 5217 5217
Size: 7627
KpnI G_GTAC'C
Cuts at: 2139 4952 2139
Size: 2813 4814
MfeI C'AATT_G
Cuts at: 3663 5226 3663
Size: 1563 6064
MluI A'CGCG_T
Cuts at: 7503 7503
Size: 7627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2431 2443 4103 6741 6925 1305
Size: 1126 12 1660 2638 184 2007
Fragments arranged by size:
2638 2007 1660 1126 184 12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4649 10
Size: 54 1425 343 2817 2988
Fragments arranged by size:
2988 2817 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2907 3594 3791 3908 5585 5944
Size: 648 1071 670 687 197 117 1677 359
Cuts at: 5944 6103 518
Size: 159 2042
Fragments arranged by size:
2042 1677 1071 687 670 648 359 197
159 117
MspA1I CmG'CkG
Cuts at: 1606 2055 2797 5043 5720 6868 7113 1606
Size: 449 742 2246 677 1148 245 2120
Fragments arranged by size:
2246 2120 1148 742 677 449 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6136
Fragments arranged by size:
6136 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7627
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7627
NotI GC'GGCC_GC
Cuts at: 5074 5074
Size: 7627
NsiI A_TGCA'T
Cuts at: 2694 3081 2694
Size: 387 7240
NspI r_CATG'y
Cuts at: 1132 1229 2194 2236 2317 2602 2696 2972 3868
Size: 97 965 42 81 285 94 276 896
Cuts at: 3868 4985 6530 1132
Size: 1117 1545 2229
Fragments arranged by size:
2229 1545 1117 965 896 285 276 97
94 81 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7627
PciI A'CATG_T
Cuts at: 2313 6526 2313
Size: 4213 3414
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3929 1108
Size: 2821 4806
PfoI T'CCnGG_A
Cuts at: 4476 4476
Size: 7627
PmeI GTTT'AAAC
Cuts at: 4931 4931
Size: 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4695 4727 5696 5728 7209 7241 4695
Size: 32 969 32 1481 32 5081
Fragments arranged by size:
5081 1481 969 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7627
PsiI TTA'TAA
Cuts at: 2676 5197 2676
Size: 2521 5106
PspXI vC'TCGA_Gb
Cuts at: 2286 2286
Size: 7627
PstI C_TGCA'G
Cuts at: 830 3709 4979 830
Size: 2879 1270 3478
Fragments arranged by size:
3478 2879 1270
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7627
PvuII CAG'CTG
Cuts at: 2055 2797 2055
Size: 742 6885
SalI G'TCGA_C
Cuts at: 1936 4969 1936
Size: 3033 4594
SbfI CC_TGCA'GG
Cuts at: 4979 4979
Size: 7627
ScaI AGT'ACT
Cuts at: 3711 4734 5875 7587 3711
Size: 1023 1141 1712 3751
Fragments arranged by size:
3751 1712 1141 1023
SexAI A'CCwGG_T
Cuts at: 3267 3451 3576 3267
Size: 184 125 7318
Fragments arranged by size:
7318 184 125
SfcI C'TryA_G
Cuts at: 826 1045 2495 2704 3125 3240 3705 4804 4975
Size: 219 1450 209 421 115 465 1099 171
Cuts at: 4975 6110 6791 6982 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1450 1135 1099 681 465 421 219
209 191 171 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7627
SmaI CCC'GGG
Cuts at: 1872 4954 1872
Size: 3082 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2286 3406 3754 4712 5731 6632
Size: 197 926 343 1120 348 958 1019 901
Cuts at: 6632 6894 820
Size: 262 1553
Fragments arranged by size:
1553 1120 1019 958 926 901 348 343
262 197
SnaBI TAC'GTA
Cuts at: 493 5418 493
Size: 4925 2702
SpeI A'CTAG_T
Cuts at: 152 3394 152
Size: 3242 4385
SphI G_CATG'C
Cuts at: 1132 2194 2972 3868 4985 1132
Size: 1062 778 896 1117 3774
Fragments arranged by size:
3774 1117 1062 896 778
SspI AAT'ATT
Cuts at: 5 52 2398 5551 5
Size: 47 2346 3153 2081
Fragments arranged by size:
3153 2346 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7627
StyI C'CwwG_G
Cuts at: 513 1065 2004 4774 5066 513
Size: 552 939 2770 292 3074
Fragments arranged by size:
3074 2770 939 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5654 5671 5824 6009 6454 325
Size: 1255 4074 17 153 185 445 1498
Fragments arranged by size:
4074 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2381 2491 3709
Size: 274 80 33 51 1443 404 110 1218
Cuts at: 3709 4270 4383 4732 5873 7585 96
Size: 561 113 349 1141 1712 138
Fragments arranged by size:
1712 1443 1218 1141 561 404 349 274
138 113 110 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2631 2663 7587 7619 2631
Size: 32 4924 32 2639
Fragments arranged by size:
4924 2639 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3602 1316
Size: 2286 5341
XbaI T'CTAG_A
Cuts at: 3559 4963 3559
Size: 1404 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3608 3608
Size: 7627
XhoI C'TCGA_G
Cuts at: 1943 2286 4712 1943
Size: 343 2426 4858
Fragments arranged by size:
4858 2426 343
XmaI C'CCGG_G
Cuts at: 1870 4952 1870
Size: 3082 4545
XmnI GAAnn'nnTTC
Cuts at: 3890 5756 3890
Size: 1866 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5435 276
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI