Restriction map for
pFN21AB5093
(Circular) (Six-base) MAPSORT of: pFN21AB5093.seq Check: 1426 from: 1 to: 7558
pFN21AB5093 7558 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5368 278
Size: 53 83 186 936 3832 2468
Fragments arranged by size:
3832 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4901 1937
Size: 2964 4594
Acc65I G'GTAC_C
Cuts at: 4879 4879
Size: 7558
AclI AA'CG_TT
Cuts at: 1563 4130 5685 6058 1563
Size: 2567 1555 373 3063
Fragments arranged by size:
3063 2567 1555 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4469 5621 7005 7273 1632
Size: 2837 1152 1384 268 1917
Fragments arranged by size:
2837 1917 1384 1152 268
AfeI AGC'GCT
Cuts at: 2187 2187
Size: 7558
AflII C'TTAA_G
Cuts at: 820 1017 2824 820
Size: 197 1807 5554
Fragments arranged by size:
5554 1807 197
AflIII A'CryG_T
Cuts at: 1203 4456 6457 7434 1203
Size: 3253 2001 977 1327
Fragments arranged by size:
3253 2001 1327 977
AgeI A'CCGG_T
Cuts at: 6389 6389
Size: 7558
AhdI GACnn_n'nnGTC
Cuts at: 6287 6287
Size: 7558
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7558
AlwNI CAG_nnn'CTG
Cuts at: 2548 2957 3855 5301 6873 2548
Size: 409 898 1446 1572 3233
Fragments arranged by size:
3233 1572 1446 898 409
ApaI G_GGCC'C
Cuts at: 2015 2067 2015
Size: 52 7506
ApaLI G'TGCA_C
Cuts at: 2513 5615 6771 2513
Size: 3102 1156 3300
Fragments arranged by size:
3300 3102 1156
ApoI r'AATT_y
Cuts at: 1493 2720 3111 3213 3445 3824 4867 5093 5245
Size: 1227 391 102 232 379 1043 226 152
Cuts at: 5245 7354 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1227 1043 391 379 232 226
152 102
AseI AT'TA_AT
Cuts at: 160 1032 3911 4163 6112 160
Size: 872 2879 252 1949 1606
Fragments arranged by size:
2879 1949 1606 872 252
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7558
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2438 4883 1870
Size: 73 216 279 2445 4545
Fragments arranged by size:
4545 2445 279 216 73
AvrII C'CTAG_G
Cuts at: 4015 4015
Size: 7558
BamHI G'GATC_C
Cuts at: 4888 5255 4888
Size: 367 7191
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2095 2506 3152 4879
Size: 325 217 627 290 18 411 646 1727
Cuts at: 4879 6334 618
Size: 1455 1842
Fragments arranged by size:
1842 1727 1455 646 627 411 325 290
217 18
BanII G_rGCy'C
Cuts at: 1391 2015 2067 2169 4877 1391
Size: 624 52 102 2708 4072
Fragments arranged by size:
4072 2708 624 102 52
BbeI G_GCGC'C
Cuts at: 2081 2099 2081
Size: 18 7540
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4440 928
Size: 971 2541 4046
Fragments arranged by size:
4046 2541 971
BbvCI CC'TCA_GC
Cuts at: 2522 2522
Size: 7558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5749 5783 1903
Size: 34 50 34 3728 34 3678
Fragments arranged by size:
3728 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2588 5450 6660 2588
Size: 2862 1210 3486
Fragments arranged by size:
3486 2862 1210
BclI T'GATC_A
Cuts at: 1548 4317 1548
Size: 2769 4789
BfrBI ATG'CAT
Cuts at: 2231 2231
Size: 7558
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2164 4007 6169
Size: 107 122 71 631 605 492 1843 2162
Cuts at: 6169 136
Size: 1525
Fragments arranged by size:
2162 1843 1525 631 605 492 122 107
71
BglII A'GATC_T
Cuts at: 2568 3187 7553 2568
Size: 619 4366 2573
Fragments arranged by size:
4366 2573 619
BlpI GC'TnA_GC
Cuts at: 2168 4975 2168
Size: 2807 4751
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2067 2160 2517 5619 6775 1790
Size: 225 52 93 357 3102 1156 2573
Fragments arranged by size:
3102 2573 1156 357 225 93 52
BmgBI CAC'GTC
Cuts at: 1318 4457 1318
Size: 3139 4419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2361 3620 5289
Size: 416 53 83 399 24 944 1259 1669
Cuts at: 5289 6247 442
Size: 958 1753
Fragments arranged by size:
1753 1669 1259 958 944 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3920 1056
Size: 2864 4694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4231 4231
Size: 7558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2705 3455 6218 1239
Size: 1085 381 750 2763 2579
Fragments arranged by size:
2763 2579 1085 750 381
Bpu10I CC'TnA_GC
Cuts at: 2522 3038 3355 2522
Size: 516 317 6725
Fragments arranged by size:
6725 516 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2557 5683 6548 6846 2557
Size: 3126 865 298 3269
Fragments arranged by size:
3269 3126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2681 3066 6221 882
Size: 617 1182 385 3155 2219
Fragments arranged by size:
3155 2219 1182 617 385
BsaAI yAC'GTr
Cuts at: 493 1204 5349 493
Size: 711 4145 2702
Fragments arranged by size:
4145 2702 711
BsaBI GATnn'nnATC
Cuts at: 2814 7552 2814
Size: 4738 2820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2096 5365 5747
Size: 53 83 186 936 545 18 3269 382
Cuts at: 5747 6382 275
Size: 635 1451
Fragments arranged by size:
3269 1451 936 635 545 382 186 83
53 18
BsaWI w'CCGG_w
Cuts at: 5991 6389 6663 6810 7221 5991
Size: 398 274 147 411 6328
Fragments arranged by size:
6328 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7333
Fragments arranged by size:
7333 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3877 1186
Size: 2691 4867
BseYI C'CCAG_C
Cuts at: 2066 3866 6761 7257 2066
Size: 1800 2895 496 2367
Fragments arranged by size:
2895 2367 1800 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2951 4793 1163
Size: 573 1215 1842 3928
Fragments arranged by size:
3928 1842 1215 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2119 2154 5008 5769 6797
Size: 275 186 60 58 35 2854 761 1028
Cuts at: 6797 1540
Size: 2301
Fragments arranged by size:
2854 2301 1028 761 275 186 60 58
35
BsiHKAI G_wGCw'C
Cuts at: 2517 5619 5704 6775 2517
Size: 3102 85 1071 3300
Fragments arranged by size:
3300 3102 1071 85
BsmI GAATG_Cn'
Cuts at: 5069 5162 5069
Size: 93 7465
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2067 2160 2169 2517 4877 5619
Size: 399 225 52 93 9 348 2360 742
Cuts at: 5619 5704 6775 1391
Size: 85 1071 2174
Fragments arranged by size:
2360 2174 1071 742 399 348 225 93
85 52 9
BspEI T'CCGG_A
Cuts at: 7221 7221
Size: 7558
BspHI T'CATG_A
Cuts at: 5447 7177 5447
Size: 1730 5828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3681 4913 844
Size: 2837 1232 3489
Fragments arranged by size:
3489 2837 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 5445 1394
Size: 429 3622 3507
Fragments arranged by size:
3622 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 6053 6227 65
Size: 1183 3128 1677 174 1396
Fragments arranged by size:
3128 1677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6202 6389 1700
Size: 110 4392 187 2869
Fragments arranged by size:
4392 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3569 3704 96
Size: 3473 135 3950
Fragments arranged by size:
3950 3473 135
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7558
BssSI C'ACGA_G
Cuts at: 5618 6630 5618
Size: 1012 6546
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2404 3855 794
Size: 1610 1451 4497
Fragments arranged by size:
4497 1610 1451
BstBI TT'CG_AA
Cuts at: 6362 6362
Size: 7558
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7558
BstYI r'GATC_y
Cuts at: 1972 2568 3187 4888 5255 5640 5657 7109 7195
Size: 596 619 1701 367 385 17 1452 86
Cuts at: 7195 7207 7553 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1701 1452 619 596 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2727 3380 4680 2727
Size: 653 1300 5605
Fragments arranged by size:
5605 1300 653
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7006
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6819
Fragments arranged by size:
6819 683 56
BtsI GCAGTG_nn'
Cuts at: 3710 3856 4320 4665 5074 5868 5888 3710
Size: 146 464 345 409 794 20 5380
Fragments arranged by size:
5380 794 464 409 345 146 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7558
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4453 4488 529
Size: 35 3889 35 3599
Fragments arranged by size:
3889 3599 35 35
DraI TTT'AAA
Cuts at: 3450 4429 4862 3450
Size: 979 433 6146
Fragments arranged by size:
6146 979 433
DraIII CAC_nnn'GTG
Cuts at: 3484 3484
Size: 7558
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6565 809
Size: 507 5249 1802
Fragments arranged by size:
5249 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2151 4167
Size: 54 1425 50 275 18 228 93 2016
Cuts at: 4167 5005 5894 7523 8
Size: 838 889 1629 43
Fragments arranged by size:
2016 1629 1425 889 838 275 228 93
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2151 5005 1537
Size: 275 246 93 2854 4090
Fragments arranged by size:
4090 2854 275 246 93
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 3962 5488 1353
Size: 897 1712 1526 3423
Fragments arranged by size:
3423 1712 1526 897
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6145 6519 6665 387
Size: 1064 138 4556 374 146 1280
Fragments arranged by size:
4556 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2705 3455 4469 5621 6218 7005
Size: 393 692 381 750 1014 1152 597 787
Cuts at: 7005 7273 1239
Size: 268 1524
Fragments arranged by size:
1524 1152 1014 787 750 692 597 393
381 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3294 3579 3294
Size: 285 7273
EcoO109I rG'GnC_Cy
Cuts at: 4210 4210
Size: 7558
EcoRI G'AATT_C
Cuts at: 4867 5245 4867
Size: 378 7180
EcoRV GAT'ATC
Cuts at: 2194 3171 2194
Size: 977 6581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2994 2994
Size: 7558
FspI TGC'GCA
Cuts at: 6064 6064
Size: 7558
HaeII r_GCGC'y
Cuts at: 1678 2081 2099 2175 2189 2549 6705 1678
Size: 403 18 76 14 360 4156 2531
Fragments arranged by size:
4156 2531 403 360 76 18 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 3172 3204 3613
Size: 32 135 32 970 32 598 32 409
Cuts at: 3613 3645 3788 3820 3961 3993 6199 6231 6273
Size: 32 143 32 141 32 2206 32 42
Cuts at: 6273 6305 1373
Size: 32 2626
Fragments arranged by size:
2626 2206 970 598 409 143 141 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2657 3376 4902 5148 669
Size: 988 281 719 719 1526 246 3079
Fragments arranged by size:
3079 1526 988 719 719 281 246
HindIII A'AGCT_T
Cuts at: 748 3934 748
Size: 3186 4372
HpaI GTT'AAC
Cuts at: 3376 5148 3376
Size: 1772 5786
KasI G'GCGC_C
Cuts at: 2077 2095 2077
Size: 18 7540
KpnI G_GTAC'C
Cuts at: 4883 4883
Size: 7558
MfeI C'AATT_G
Cuts at: 2264 4270 5157 2264
Size: 2006 887 4665
Fragments arranged by size:
4665 2006 887
MluI A'CGCG_T
Cuts at: 7434 7434
Size: 7558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 6672 6856 1305
Size: 3414 1953 184 2007
Fragments arranged by size:
3414 2007 1953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5736
Fragments arranged by size:
5736 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2841 5516 5875 6034 518
Size: 648 1675 2675 359 159 2042
Fragments arranged by size:
2675 2042 1675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2106 4644 4974 5651 6799 7044 1606
Size: 464 36 2538 330 677 1148 245 2120
Fragments arranged by size:
2538 2120 1148 677 464 330 245 36
NarI GG'CG_CC
Cuts at: 2078 2096 2078
Size: 18 7540
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7006
NdeI CA'TA_TG
Cuts at: 387 3400 387
Size: 3013 4545
NheI G'CTAG_C
Cuts at: 1052 3916 1052
Size: 2864 4694
NotI GC'GGCC_GC
Cuts at: 5005 5005
Size: 7558
NsiI A_TGCA'T
Cuts at: 2233 2233
Size: 7558
NspI r_CATG'y
Cuts at: 1132 1229 4250 4546 4916 6461 1132
Size: 97 3021 296 370 1545 2229
Fragments arranged by size:
3021 2229 1545 370 296 97
PasI CC'CwG_GG
Cuts at: 1348 2041 1348
Size: 693 6865
PciI A'CATG_T
Cuts at: 6457 6457
Size: 7558
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 3103 3484 1108
Size: 1080 915 381 5182
Fragments arranged by size:
5182 1080 915 381
PfoI T'CCnGG_A
Cuts at: 2135 3471 2135
Size: 1336 6222
PmeI GTTT'AAAC
Cuts at: 4862 4862
Size: 7558
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5627 5659 7140 7172 5627
Size: 32 1481 32 6013
Fragments arranged by size:
6013 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4210 4210
Size: 7558
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7558
PsiI TTA'TAA
Cuts at: 2706 5128 2706
Size: 2422 5136
PspOMI G'GGCC_C
Cuts at: 2011 2063 2011
Size: 52 7506
PstI C_TGCA'G
Cuts at: 830 2105 4910 830
Size: 1275 2805 3478
Fragments arranged by size:
3478 2805 1275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7558
PvuII CAG'CTG
Cuts at: 2106 4644 2106
Size: 2538 5020
SalI G'TCGA_C
Cuts at: 1936 4900 1936
Size: 2964 4594
SapI GCTCTTCn'nnn_
Cuts at: 2250 3962 2250
Size: 1712 5846
SbfI CC_TGCA'GG
Cuts at: 4910 4910
Size: 7558
ScaI AGT'ACT
Cuts at: 5806 7518 5806
Size: 1712 5846
SexAI A'CCwGG_T
Cuts at: 4767 4767
Size: 7558
SfcI C'TryA_G
Cuts at: 826 1045 2101 2540 4023 4095 4906 6041 6722
Size: 219 1056 439 1483 72 811 1135 681
Cuts at: 6722 6913 826
Size: 191 1471
Fragments arranged by size:
1483 1471 1135 1056 811 681 439 219
191 72
SfoI GGC'GCC
Cuts at: 2079 2097 2079
Size: 18 7540
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7558
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7558
SmaI CCC'GGG
Cuts at: 1872 4885 1872
Size: 3013 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2572 2824 5662 6563 6825 820
Size: 197 926 629 252 2838 901 262 1553
Fragments arranged by size:
2838 1553 926 901 629 262 252 197
SnaBI TAC'GTA
Cuts at: 493 5349 493
Size: 4856 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7558
SphI G_CATG'C
Cuts at: 1132 4916 1132
Size: 3784 3774
SspI AAT'ATT
Cuts at: 5 52 3605 4064 5482 5
Size: 47 3553 459 1418 2081
Fragments arranged by size:
3553 2081 1418 459 47
StuI AGG'CCT
Cuts at: 1517 2303 3165 3300 4778 1517
Size: 786 862 135 1478 4297
Fragments arranged by size:
4297 1478 862 786 135
StyI C'CwwG_G
Cuts at: 513 1065 4015 4501 4997 513
Size: 552 2950 486 496 3074
Fragments arranged by size:
3074 2950 552 496 486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2195 2310 5585 5602 5755 5940 6385
Size: 1255 615 115 3275 17 153 185 445
Cuts at: 6385 325
Size: 1498
Fragments arranged by size:
3275 1498 1255 615 445 185 153 115
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 3704 4545
Size: 274 80 33 51 1443 1592 135 841
Cuts at: 4545 5804 7516 96
Size: 1259 1712 138
Fragments arranged by size:
1712 1592 1443 1259 841 274 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7518 7550 7518
Size: 32 7526
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7558
XbaI T'CTAG_A
Cuts at: 2251 3184 4894 2251
Size: 933 1710 4915
Fragments arranged by size:
4915 1710 933
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7558
XmaI C'CCGG_G
Cuts at: 1870 4883 1870
Size: 3013 4545
XmnI GAAnn'nnTTC
Cuts at: 2376 4593 5687 2376
Size: 2217 1094 4247
Fragments arranged by size:
4247 2217 1094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5366 276
Size: 53 83 186 936 3832 2468
Fragments arranged by size:
3832 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BsiWI BsmBI BstXI BstZ17I
EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI
SwaI XcmI