Restriction map for
pFN21AB5091
(Circular) (Six-base) MAPSORT of: pFN21AB5091.seq Check: 8972 from: 1 to: 6517
pFN21AB5091 6517 bp
With 182 enzymes: SgfI *
October 20, 2009 10:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4327 278
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3860 1937
Size: 1923 4594
Acc65I G'GTAC_C
Cuts at: 3838 3838
Size: 6517
AclI AA'CG_TT
Cuts at: 1563 2262 3802 4644 5017 1563
Size: 699 1540 842 373 3063
Fragments arranged by size:
3063 1540 842 699 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 4580 5964 6232 1632
Size: 1766 1182 1384 268 1917
Fragments arranged by size:
1917 1766 1384 1182 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 5416 6393 1203
Size: 4213 977 1327
Fragments arranged by size:
4213 1327 977
AgeI A'CCGG_T
Cuts at: 5348 5348
Size: 6517
AhdI GACnn_n'nnGTC
Cuts at: 5246 5246
Size: 6517
AleI CACnn'nnGTG
Cuts at: 1166 3018 1166
Size: 1852 4665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2080 1184
Size: 896 5621
AlwNI CAG_nnn'CTG
Cuts at: 4260 5832 4260
Size: 1572 4945
ApaLI G'TGCA_C
Cuts at: 2225 4574 5730 2225
Size: 2349 1156 3012
Fragments arranged by size:
3012 2349 1156
ApoI r'AATT_y
Cuts at: 1493 2177 3041 3101 3150 3826 4052 4204 6313
Size: 684 864 60 49 676 226 152 2109
Cuts at: 6313 1493
Size: 1697
Fragments arranged by size:
2109 1697 864 684 676 226 152 60
49
AseI AT'TA_AT
Cuts at: 160 1032 5071 160
Size: 872 4039 1606
Fragments arranged by size:
4039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 3842 1870
Size: 73 1899 4545
Fragments arranged by size:
4545 1899 73
BamHI G'GATC_C
Cuts at: 3847 4214 3847
Size: 367 6150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 3838 5293 618
Size: 325 217 627 1058 993 1455 1842
Fragments arranged by size:
1842 1455 1058 993 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2416 2543 3723 3836 1391
Size: 1025 127 1180 113 4072
Fragments arranged by size:
4072 1180 1025 127 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4708 4742 1903
Size: 34 50 34 2687 34 3678
Fragments arranged by size:
3678 2687 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2757 3483 4409 5619 2757
Size: 726 926 1210 3655
Fragments arranged by size:
3655 1210 926 726
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5128 136
Size: 107 122 71 631 605 3456 1525
Fragments arranged by size:
3456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6512 6512
Size: 6517
BlpI GC'TnA_GC
Cuts at: 2045 3934 2045
Size: 1889 4628
Bme1580I G_kGCm'C
Cuts at: 1790 2229 4578 5734 1790
Size: 439 2349 1156 2573
Fragments arranged by size:
2573 2349 1156 439
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3217 4248 5206
Size: 416 53 83 399 24 1800 1031 958
Cuts at: 5206 442
Size: 1753
Fragments arranged by size:
1800 1753 1031 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5177 1239
Size: 3938 2579
Bpu10I CC'TnA_GC
Cuts at: 2802 2802
Size: 6517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2778 3037 4642 5507 5805 2778
Size: 259 1605 865 298 3490
Fragments arranged by size:
3490 1605 865 298 259
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5180 882
Size: 617 3681 2219
Fragments arranged by size:
3681 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4308 493
Size: 711 3104 2702
Fragments arranged by size:
3104 2702 711
BsaBI GATnn'nnATC
Cuts at: 3777 6511 3777
Size: 2734 3783
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4324 4706 5341 275
Size: 53 83 186 936 2791 382 635 1451
Fragments arranged by size:
2791 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2173 3761 4950 5348 5622 5769 6180 2173
Size: 1588 1189 398 274 147 411 2510
Fragments arranged by size:
2510 1588 1189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6292
Fragments arranged by size:
6292 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2781 1186
Size: 1595 4922
BseYI C'CCAG_C
Cuts at: 2376 2568 2632 5720 6216 2376
Size: 192 64 3088 496 2677
Fragments arranged by size:
3088 2677 496 192 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5944
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4728 5756 1540
Size: 275 186 1966 761 1028 2301
Fragments arranged by size:
2301 1966 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 3544 3723 4578 4663 5734 2229
Size: 1315 179 855 85 1071 3012
Fragments arranged by size:
3012 1315 1071 855 179 85
BsmI GAATG_Cn'
Cuts at: 2558 4028 4121 2558
Size: 1470 93 4954
Fragments arranged by size:
4954 1470 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2416 2543 3544 3723 3836 4578
Size: 399 439 187 127 1001 179 113 742
Cuts at: 4578 4663 5734 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1001 742 439 399 187 179
127 113 85
BspEI T'CCGG_A
Cuts at: 3761 6180 3761
Size: 2419 4098
BspHI T'CATG_A
Cuts at: 3154 4406 6136 3154
Size: 1252 1730 3535
Fragments arranged by size:
3535 1730 1252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3872 844
Size: 3028 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 4404 1394
Size: 429 1936 645 3507
Fragments arranged by size:
3507 1936 645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5012 5186 65
Size: 1183 3764 174 1396
Fragments arranged by size:
3764 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5161 5348 1700
Size: 110 3351 187 2869
Fragments arranged by size:
3351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 4577 5589 4577
Size: 1012 5505
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2564 794
Size: 1770 4747
BstBI TT'CG_AA
Cuts at: 2287 5321 2287
Size: 3034 3483
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6517
BstXI CCAn_nnnn'nTGG
Cuts at: 2632 2632
Size: 6517
BstYI r'GATC_y
Cuts at: 1972 3712 3847 4214 4599 4616 6068 6154 6166
Size: 1740 135 367 385 17 1452 86 12
Cuts at: 6166 6512 1972
Size: 346 1977
Fragments arranged by size:
1977 1740 1452 385 367 346 135 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2638 3525 513
Size: 552 1573 887 3505
Fragments arranged by size:
3505 1573 887 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2916 487
Size: 683 56 1690 4088
Fragments arranged by size:
4088 1690 683 56
BtsI GCAGTG_nn'
Cuts at: 3642 4033 4827 4847 3642
Size: 391 794 20 5312
Fragments arranged by size:
5312 794 391 20
ClaI AT'CG_AT
Cuts at: 1554 3456 1554
Size: 1902 4615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 3821 3821
Size: 6517
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5524 809
Size: 507 4208 1802
Fragments arranged by size:
4208 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3500 3964 4853
Size: 54 1425 50 275 18 1670 464 889
Cuts at: 4853 6482 8
Size: 1629 43
Fragments arranged by size:
1670 1629 1425 889 464 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4447 1353
Size: 3094 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5104 5478 5624 387
Size: 1064 138 3515 374 146 1280
Fragments arranged by size:
3515 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3721 3721
Size: 6517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3398 4580 5177 5964 6232 1239
Size: 393 1766 1182 597 787 268 1524
Fragments arranged by size:
1766 1524 1182 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2702 2702
Size: 6517
EcoRI G'AATT_C
Cuts at: 3826 4204 3826
Size: 378 6139
EcoRV GAT'ATC
Cuts at: 3775 3775
Size: 6517
FspI TGC'GCA
Cuts at: 5023 5023
Size: 6517
HaeII r_GCGC'y
Cuts at: 1678 2045 5664 1678
Size: 367 3619 2531
Fragments arranged by size:
3619 2531 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2355 2734 2766 2773
Size: 32 135 32 751 32 379 32 7
Cuts at: 2773 2805 3010 3042 5158 5190 5232 5264 1373
Size: 32 205 32 2116 32 42 32 2626
Fragments arranged by size:
2626 2116 751 379 205 135 42 32
32 32 32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2313 3861 4107 669
Size: 988 281 375 1548 246 3079
Fragments arranged by size:
3079 1548 988 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6517
HpaI GTT'AAC
Cuts at: 4107 4107
Size: 6517
KpnI G_GTAC'C
Cuts at: 3842 3842
Size: 6517
MfeI C'AATT_G
Cuts at: 4116 4116
Size: 6517
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3414 3517 5631 5815 1305
Size: 2109 103 2114 184 2007
Fragments arranged by size:
2114 2109 2007 184 103
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4695
Fragments arranged by size:
4695 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3018 4475 4834 4993 518
Size: 648 1852 1457 359 159 2042
Fragments arranged by size:
2042 1852 1457 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3933 4610 5758 6003 1606
Size: 2327 677 1148 245 2120
Fragments arranged by size:
2327 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2638 3525 513
Size: 552 1573 887 3505
Fragments arranged by size:
3505 1573 887 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 3875 5420 1132
Size: 97 2646 1545 2229
Fragments arranged by size:
2646 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2536 1348
Size: 1188 5329
PciI A'CATG_T
Cuts at: 5416 5416
Size: 6517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 2874 1108
Size: 1536 230 4751
Fragments arranged by size:
4751 1536 230
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 4586 4618 6099 6131 2930
Size: 32 1624 32 1481 32 3316
Fragments arranged by size:
3316 1624 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6517
PsiI TTA'TAA
Cuts at: 4087 4087
Size: 6517
PstI C_TGCA'G
Cuts at: 830 3430 3869 830
Size: 2600 439 3478
Fragments arranged by size:
3478 2600 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
SacI G_AGCT'C
Cuts at: 3723 3723
Size: 6517
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SbfI CC_TGCA'GG
Cuts at: 3869 3869
Size: 6517
ScaI AGT'ACT
Cuts at: 3789 4765 6477 3789
Size: 976 1712 3829
Fragments arranged by size:
3829 1712 976
SfcI C'TryA_G
Cuts at: 826 1045 3426 3865 5000 5681 5872 826
Size: 219 2381 439 1135 681 191 1471
Fragments arranged by size:
2381 1471 1135 681 439 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6517
SmaI CCC'GGG
Cuts at: 1872 3844 1872
Size: 1972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2793 3016 4621 5522 5784 820
Size: 197 926 850 223 1605 901 262 1553
Fragments arranged by size:
1605 1553 926 901 850 262 223 197
SnaBI TAC'GTA
Cuts at: 493 4308 493
Size: 3815 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6517
SphI G_CATG'C
Cuts at: 1132 3875 1132
Size: 2743 3774
SspI AAT'ATT
Cuts at: 5 52 4441 5
Size: 47 4389 2081
Fragments arranged by size:
4389 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6517
StyI C'CwwG_G
Cuts at: 513 1065 2448 2638 3525 3956 513
Size: 552 1383 190 887 431 3074
Fragments arranged by size:
3074 1383 887 552 431 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2091 2441 4544 4561 4714 4899 5344
Size: 1255 511 350 2103 17 153 185 445
Cuts at: 5344 325
Size: 1498
Fragments arranged by size:
2103 1498 1255 511 445 350 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3292 3787 4763
Size: 274 80 33 51 1443 1315 495 976
Cuts at: 4763 6475 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1315 976 495 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6477 6509 6477
Size: 32 6485
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6517
XbaI T'CTAG_A
Cuts at: 3853 3853
Size: 6517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3842 1870
Size: 1972 4545
XmnI GAAnn'nnTTC
Cuts at: 4646 4646
Size: 6517
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4325 276
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI