Restriction map for
pFN21AB5086
(Circular) (Six-base) MAPSORT of: pFN21AB5086.seq Check: 8794 from: 1 to: 6685
pFN21AB5086 6685 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4495 278
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3755 4028 1937
Size: 1818 273 4594
Fragments arranged by size:
4594 1818 273
Acc65I G'GTAC_C
Cuts at: 4006 4006
Size: 6685
AclI AA'CG_TT
Cuts at: 1563 3078 4812 5185 1563
Size: 1515 1734 373 3063
Fragments arranged by size:
3063 1734 1515 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2963 2984 3022 3125 3392 4748 6132 6400
Size: 1331 21 38 103 267 1356 1384 268
Cuts at: 6400 1632
Size: 1917
Fragments arranged by size:
1917 1384 1356 1331 268 267 103 38
21
AfeI AGC'GCT
Cuts at: 3386 3386
Size: 6685
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6488
AflIII A'CryG_T
Cuts at: 1203 2910 5584 6561 1203
Size: 1707 2674 977 1327
Fragments arranged by size:
2674 1707 1327 977
AgeI A'CCGG_T
Cuts at: 5516 5516
Size: 6685
AhdI GACnn_n'nnGTC
Cuts at: 5414 5414
Size: 6685
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3341 3885 1184
Size: 2157 544 3984
Fragments arranged by size:
3984 2157 544
AlwNI CAG_nnn'CTG
Cuts at: 2959 3646 3981 4428 6000 2959
Size: 687 335 447 1572 3644
Fragments arranged by size:
3644 1572 687 447 335
ApaI G_GGCC'C
Cuts at: 3269 3770 3797 3269
Size: 501 27 6157
Fragments arranged by size:
6157 501 27
ApaLI G'TGCA_C
Cuts at: 3515 4742 5898 3515
Size: 1227 1156 4302
Fragments arranged by size:
4302 1227 1156
ApoI r'AATT_y
Cuts at: 1493 3994 4220 4372 6481 1493
Size: 2501 226 152 2109 1697
Fragments arranged by size:
2501 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5239 160
Size: 872 4207 1606
Fragments arranged by size:
4207 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2053 3193 4010 1870
Size: 73 96 14 1140 817 4545
Fragments arranged by size:
4545 1140 817 96 73 14
BamHI G'GATC_C
Cuts at: 4015 4382 4015
Size: 367 6318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3680 4006 5461 618
Size: 325 217 627 1893 326 1455 1842
Fragments arranged by size:
1893 1842 1455 627 326 325 217
BanII G_rGCy'C
Cuts at: 1391 2046 2186 3269 3770 3797 4004 1391
Size: 655 140 1083 501 27 207 4072
Fragments arranged by size:
4072 1083 655 501 207 140 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2761 928
Size: 971 862 4852
Fragments arranged by size:
4852 971 862
BbvCI CC'TCA_GC
Cuts at: 3891 3891
Size: 6685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4876 4910 1903
Size: 34 2939 34 3678
Fragments arranged by size:
3678 2939 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5787 4577
Size: 1210 5475
BclI T'GATC_A
Cuts at: 1548 3237 1548
Size: 1689 4996
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 2673 5296
Size: 107 122 71 631 605 403 598 2623
Cuts at: 5296 136
Size: 1525
Fragments arranged by size:
2623 1525 631 605 598 403 122 107
71
BglII A'GATC_T
Cuts at: 2694 3069 3097 6680 2694
Size: 375 28 3583 2699
Fragments arranged by size:
3583 2699 375 28
BlpI GC'TnA_GC
Cuts at: 4102 4102
Size: 6685
Bme1580I G_kGCm'C
Cuts at: 1790 3059 3269 3519 3770 3797 4746 5902 1790
Size: 1269 210 250 251 27 949 1156 2573
Fragments arranged by size:
2573 1269 1156 949 251 250 210 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4416 5374
Size: 416 53 83 399 24 2171 828 958
Cuts at: 5374 442
Size: 1753
Fragments arranged by size:
2171 1753 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 2702 3683 3852 3869 5345 1239
Size: 1235 228 981 169 17 1476 2579
Fragments arranged by size:
2579 1476 1235 981 228 169 17
Bpu10I CC'TnA_GC
Cuts at: 3891 3897 3891
Size: 6 6679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 5675 5973 4810
Size: 865 298 5522
Fragments arranged by size:
5522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2246 5348 882
Size: 617 747 3102 2219
Fragments arranged by size:
3102 2219 747 617
BsaAI yAC'GTr
Cuts at: 493 1204 4476 493
Size: 711 3272 2702
Fragments arranged by size:
3272 2702 711
BsaBI GATnn'nnATC
Cuts at: 6679 6679
Size: 6685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4492 4874 5509 275
Size: 53 83 186 936 2959 382 635 1451
Fragments arranged by size:
2959 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2424 3396 5118 5516 5790 5937 6348 2424
Size: 972 1722 398 274 147 411 2761
Fragments arranged by size:
2761 1722 972 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3110 3140 3170 3200 1178
Size: 30 165 30 1707 30 30 30 4663
Fragments arranged by size:
4663 1707 165 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3113 3164 3167 3686 3835 1186
Size: 1927 51 3 519 149 4036
Fragments arranged by size:
4036 1927 519 149 51 3
BseYI C'CCAG_C
Cuts at: 2045 2396 3262 3902 5888 6384 2045
Size: 351 866 640 1986 496 2346
Fragments arranged by size:
2346 1986 866 640 496 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3020 3614 1163
Size: 573 1284 594 4234
Fragments arranged by size:
4234 1284 594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 4135 4896 5924 1540
Size: 275 186 78 2056 761 1028 2301
Fragments arranged by size:
2301 2056 1028 761 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 3519 3823 4746 4831 5902 3519
Size: 304 923 85 1071 4302
Fragments arranged by size:
4302 1071 923 304 85
BsmI GAATG_Cn'
Cuts at: 4196 4289 4196
Size: 93 6592
BsmBI CGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 6685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2186 3059 3269 3519 3770 3797
Size: 399 256 140 873 210 250 251 27
Cuts at: 3797 3823 4004 4746 4831 5902 1391
Size: 26 181 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 399 256 251 250
210 181 140 85 27 26
BspEI T'CCGG_A
Cuts at: 2424 3396 6348 2424
Size: 972 2952 2761
Fragments arranged by size:
2952 2761 972
BspHI T'CATG_A
Cuts at: 2743 4574 6304 2743
Size: 1831 1730 3124
Fragments arranged by size:
3124 1831 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3636 4040 844
Size: 2792 404 3489
Fragments arranged by size:
3489 2792 404
BsrBI CCG'CTC
Cuts at: 1394 1823 4572 1394
Size: 429 2749 3507
Fragments arranged by size:
3507 2749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2650 2827 3294 5180 5354 65
Size: 1183 1402 177 467 1886 174 1396
Fragments arranged by size:
1886 1402 1396 1183 467 177 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 3915 5329 5516 1700
Size: 110 204 1901 1414 187 2869
Fragments arranged by size:
2869 1901 1414 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 3595 96
Size: 3499 3186
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 4745 5757 4745
Size: 1012 5673
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3365 794
Size: 2571 4114
BstBI TT'CG_AA
Cuts at: 5489 5489
Size: 6685
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6685
BstXI CCAn_nnnn'nTGG
Cuts at: 2170 2608 2170
Size: 438 6247
BstYI r'GATC_y
Cuts at: 1972 2694 2985 3069 3097 4015 4382 4767 4784
Size: 722 291 84 28 918 367 385 17
Cuts at: 4784 6236 6322 6334 6680 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 918 722 385 367 346 291
86 84 28 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2092 2668 3510 513
Size: 552 939 66 22 576 842 3688
Fragments arranged by size:
3688 939 842 576 552 66 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3299 487
Size: 683 56 2073 3873
Fragments arranged by size:
3873 2073 683 56
BtsI GCAGTG_nn'
Cuts at: 2475 2611 3913 4201 4995 5015 2475
Size: 136 1302 288 794 20 4145
Fragments arranged by size:
4145 1302 794 288 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6650
DraI TTT'AAA
Cuts at: 3989 3989
Size: 6685
DraIII CAC_nnn'GTG
Cuts at: 3943 3943
Size: 6685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 5692 809
Size: 507 699 3677 1802
Fragments arranged by size:
3677 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2076 2539
Size: 54 1425 50 275 18 231 15 463
Cuts at: 2539 3349 4132 5021 6650 8
Size: 810 783 889 1629 43
Fragments arranged by size:
1629 1425 889 810 783 463 275 231
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2076 4132 1537
Size: 275 264 2056 4090
Fragments arranged by size:
4090 2056 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2570 4615 1353
Size: 980 237 2045 3423
Fragments arranged by size:
3423 2045 980 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2363 2558 5272 5646 5792 387
Size: 1064 138 774 195 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 774 374 195 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2474 2702 2963 2984 3022 3125 3392
Size: 393 842 228 261 21 38 103 267
Cuts at: 3392 3683 3852 3869 4748 5345 6132 6400 1239
Size: 291 169 17 879 597 787 268 1524
Fragments arranged by size:
1524 879 842 787 597 393 291 268
267 261 228 169 103 38 21 17
EcoO109I rG'GnC_Cy
Cuts at: 2512 3766 3767 3793 3957 2512
Size: 1254 1 26 164 5240
Fragments arranged by size:
5240 1254 164 26 1
EcoRI G'AATT_C
Cuts at: 3994 4372 3994
Size: 378 6307
FspI TGC'GCA
Cuts at: 3575 5191 3575
Size: 1616 5069
HaeII r_GCGC'y
Cuts at: 1678 2135 2959 3388 3937 5832 1678
Size: 457 824 429 549 1895 2531
Fragments arranged by size:
2531 1895 824 549 457 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2802 3170 3202 3222
Size: 32 135 32 1198 32 368 32 20
Cuts at: 3222 3254 3827 3859 5326 5358 5400 5432 1373
Size: 32 573 32 1467 32 42 32 2626
Fragments arranged by size:
2626 1467 1198 573 368 135 42 32
32 32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4029 4275 669
Size: 988 281 1818 273 246 3079
Fragments arranged by size:
3079 1818 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6685
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KpnI G_GTAC'C
Cuts at: 4010 4010
Size: 6685
MfeI C'AATT_G
Cuts at: 4284 4284
Size: 6685
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3057 3099 5799 5983 1305
Size: 1752 42 2700 184 2007
Fragments arranged by size:
2700 2007 1752 184 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3351 10
Size: 54 1425 343 1519 3344
Fragments arranged by size:
3344 1519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 2807 3410 4643 5002 5161 518
Size: 648 1411 230 603 1233 359 159 2042
Fragments arranged by size:
2042 1411 1233 648 603 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 2072 3643 3869 3978 4101 4778 5926 6171
Size: 466 1571 226 109 123 677 1148 245
Cuts at: 6171 1606
Size: 2120
Fragments arranged by size:
2120 1571 1148 677 466 245 226 123
109
NaeI GCC'GGC
Cuts at: 3917 3917
Size: 6685
NcoI C'CATG_G
Cuts at: 513 1065 2004 3510 513
Size: 552 939 1506 3688
Fragments arranged by size:
3688 1506 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6685
NgoMIV G'CCGG_C
Cuts at: 3915 3915
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 2076 4132 2076
Size: 2056 4629
NspI r_CATG'y
Cuts at: 1132 1229 2806 2914 3328 4043 5588 1132
Size: 97 1577 108 414 715 1545 2229
Fragments arranged by size:
2229 1577 1545 715 414 108 97
PasI CC'CwG_GG
Cuts at: 1348 3952 1348
Size: 2604 4081
PciI A'CATG_T
Cuts at: 2910 5584 2910
Size: 2674 4011
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3511 3646 1108
Size: 2403 135 4147
Fragments arranged by size:
4147 2403 135
PfoI T'CCnGG_A
Cuts at: 2451 3654 2451
Size: 1203 5482
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 2512
Size: 6685
PshAI GACnn'nnGTC
Cuts at: 1613 2913 1613
Size: 1300 5385
PsiI TTA'TAA
Cuts at: 4255 4255
Size: 6685
PspOMI G'GGCC_C
Cuts at: 3265 3766 3793 3265
Size: 501 27 6157
Fragments arranged by size:
6157 501 27
PstI C_TGCA'G
Cuts at: 830 2117 4037 830
Size: 1287 1920 3478
Fragments arranged by size:
3478 1920 1287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
PvuII CAG'CTG
Cuts at: 3869 3869
Size: 6685
SacII CC_GC'GG
Cuts at: 2073 2073
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 3754 4027 1936
Size: 1818 273 4594
Fragments arranged by size:
4594 1818 273
SapI GCTCTTCn'nnn_
Cuts at: 2333 2333
Size: 6685
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SexAI A'CCwGG_T
Cuts at: 2418 2418
Size: 6685
SfcI C'TryA_G
Cuts at: 826 1045 2113 3142 3849 4033 5168 5849 6040
Size: 219 1068 1029 707 184 1135 681 191
Cuts at: 6040 826
Size: 1471
Fragments arranged by size:
1471 1135 1068 1029 707 681 219 191
184
SfiI GGCCn_nnn'nGGCC
Cuts at: 2075 2075
Size: 6685
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 2055 3195 4012 1872
Size: 183 1140 817 4545
Fragments arranged by size:
4545 1140 817 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4789 5690 5952 820
Size: 197 926 2846 901 262 1553
Fragments arranged by size:
2846 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4476 493
Size: 3983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 2806 3328 4043 1132
Size: 1674 522 715 3774
Fragments arranged by size:
3774 1674 715 522
SspI AAT'ATT
Cuts at: 5 52 4609 5
Size: 47 4557 2081
Fragments arranged by size:
4557 2081 47
StuI AGG'CCT
Cuts at: 1517 2321 3234 1517
Size: 804 913 4968
Fragments arranged by size:
4968 913 804
StyI C'CwwG_G
Cuts at: 513 1065 2004 3276 3510 3669 4124 513
Size: 552 939 1272 234 159 455 3074
Fragments arranged by size:
3074 1272 939 552 455 234 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4712 4729 4882 5067 5512 325
Size: 1255 3132 17 153 185 445 1498
Fragments arranged by size:
3132 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3595 4931 6643
Size: 274 80 33 51 1443 1618 1336 1712
Cuts at: 6643 96
Size: 138
Fragments arranged by size:
1712 1618 1443 1336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6645 6677 6645
Size: 32 6653
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6685
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2851 3553 2851
Size: 702 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 2053 3193 4010 1870
Size: 183 1140 817 4545
Fragments arranged by size:
4545 1140 817 183
XmnI GAAnn'nnTTC
Cuts at: 2564 3110 4814 2564
Size: 546 1704 4435
Fragments arranged by size:
4435 1704 546
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4493 276
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SanDI SfoI SrfI SwaI