Restriction map for
pFN21AB5081
(Circular) (Six-base) MAPSORT of: pFN21AB5081.seq Check: 1530 from: 1 to: 6328
pFN21AB5081 6328 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3354 3393 3671 1937
Size: 1417 39 278 4594
Fragments arranged by size:
4594 1417 278 39
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2775 4391 5775 6043 1632
Size: 1143 1616 1384 268 1917
Fragments arranged by size:
1917 1616 1384 1143 268
AfeI AGC'GCT
Cuts at: 2258 2258
Size: 6328
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 3396 5159 3396
Size: 1763 4565
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2558 1184
Size: 1374 4954
AlwNI CAG_nnn'CTG
Cuts at: 3491 4071 5643 3491
Size: 580 1572 4176
Fragments arranged by size:
4176 1572 580
ApaI G_GGCC'C
Cuts at: 2252 3585 2252
Size: 1333 4995
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 3637 3863 4015 6124 1493
Size: 2144 226 152 2109 1697
Fragments arranged by size:
2144 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2127 2278 3653 1870
Size: 73 79 105 151 1375 4545
Fragments arranged by size:
4545 1375 151 105 79 73
BamHI G'GATC_C
Cuts at: 3526 3658 4025 3526
Size: 132 367 5829
Fragments arranged by size:
5829 367 132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2348 2387 2471 2662 3149
Size: 325 217 627 561 39 84 191 487
Cuts at: 3149 3170 3649 5104 618
Size: 21 479 1455 1842
Fragments arranged by size:
1842 1455 627 561 487 479 325 217
191 84 39 21
BanII G_rGCy'C
Cuts at: 1391 2040 2055 2252 2958 3585 3647 1391
Size: 649 15 197 706 627 62 4072
Fragments arranged by size:
4072 706 649 627 197 62 15
BbeI G_GCGC'C
Cuts at: 2352 2391 2475 3153 2352
Size: 39 84 678 5527
Fragments arranged by size:
5527 678 84 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 928
Size: 971 367 4990
Fragments arranged by size:
4990 971 367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2323 2357 4519 4553 1903
Size: 34 386 34 2162 34 3678
Fragments arranged by size:
3678 2162 386 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 2310 1548
Size: 762 5566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2192 2201
Size: 107 122 71 631 605 337 183 9
Cuts at: 2201 2385 2671 4939 136
Size: 184 286 2268 1525
Fragments arranged by size:
2268 1525 631 605 337 286 184 183
122 107 71 9
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 3215 3745 3215
Size: 530 5798
Bme1580I G_kGCm'C
Cuts at: 1790 2252 2667 3585 4389 5545 1790
Size: 462 415 918 804 1156 2573
Fragments arranged by size:
2573 1156 918 804 462 415
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 4059 5017
Size: 416 53 83 399 24 805 1837 958
Cuts at: 5017 442
Size: 1753
Fragments arranged by size:
1837 1753 958 805 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3329
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3324 4988 1239
Size: 2085 1664 2579
Fragments arranged by size:
2579 2085 1664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2281 4453 5318 5616 2281
Size: 2172 865 298 2993
Fragments arranged by size:
2993 2172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2818 4991 882
Size: 617 1319 2173 2219
Fragments arranged by size:
2219 2173 1319 617
BsaAI yAC'GTr
Cuts at: 493 1204 3460 4119 493
Size: 711 2256 659 2702
Fragments arranged by size:
2702 2256 711 659
BsaBI GATnn'nnATC
Cuts at: 2312 2693 6322 2312
Size: 381 3629 2318
Fragments arranged by size:
3629 2318 381
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 2388 2429 2472
Size: 53 83 186 936 816 39 41 43
Cuts at: 2472 3029 3150 4135 4517 5152 275
Size: 557 121 985 382 635 1451
Fragments arranged by size:
1451 985 936 816 635 557 382 186
121 83 53 43 41 39
BsaWI w'CCGG_w
Cuts at: 2645 3396 4761 5159 5433 5580 5991 2645
Size: 751 1365 398 274 147 411 2982
Fragments arranged by size:
2982 1365 751 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3347 3377 1178
Size: 30 165 30 1944 30 4129
Fragments arranged by size:
4129 1944 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2010 3113 3437 3554 1186
Size: 824 1103 324 117 3960
Fragments arranged by size:
3960 1103 824 324 117
BseYI C'CCAG_C
Cuts at: 2206 2531 2746 2935 5531 6027 2206
Size: 325 215 189 2596 496 2507
Fragments arranged by size:
2596 2507 496 325 215 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2353 1163
Size: 573 617 5138
Fragments arranged by size:
5138 617 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2380 3354 3397 3603 3778 4539
Size: 275 186 379 974 43 206 175 761
Cuts at: 4539 5567 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 974 761 379 275 206 186
175 43
BsiHKAI G_wGCw'C
Cuts at: 2547 4389 4474 5545 2547
Size: 1842 85 1071 3330
Fragments arranged by size:
3330 1842 1071 85
BsiWI C'GTAC_G
Cuts at: 3467 3467
Size: 6328
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2055 2252 2547 2667 2958 3585
Size: 399 250 15 197 295 120 291 627
Cuts at: 3585 3647 4389 4474 5545 1391
Size: 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 627 399 295 291 250
197 120 85 62 15
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2169 3683 844
Size: 1325 1514 3489
Fragments arranged by size:
3489 1514 1325
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3204 4823 4997 65
Size: 1183 1956 1619 174 1396
Fragments arranged by size:
1956 1619 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 3396 4972 5159 1700
Size: 110 385 1201 1576 187 2869
Fragments arranged by size:
2869 1576 1201 385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 2370 1925
Size: 445 5883
BssSI C'ACGA_G
Cuts at: 4388 5400 4388
Size: 1012 5316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3593 794
Size: 2799 3529
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 2413 1184
Size: 1229 5099
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2809 2425
Size: 384 5944
BstYI r'GATC_y
Cuts at: 1972 2560 3526 3658 4025 4410 4427 5879 5965
Size: 588 966 132 367 385 17 1452 86
Cuts at: 5965 5977 6323 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 966 588 385 367 346 132
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2499 2508 2667 2802 2950
Size: 552 939 183 312 9 159 135 148
Cuts at: 2950 3597 513
Size: 647 3244
Fragments arranged by size:
3244 939 647 552 312 183 159 148
135 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 3039 3204 3844 4638 4658 2521
Size: 518 165 640 794 20 4191
Fragments arranged by size:
4191 794 640 518 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3028 3063 529
Size: 35 2464 35 3794
Fragments arranged by size:
3794 2464 35 35
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3389 5335 809
Size: 507 2073 1946 1802
Fragments arranged by size:
2073 1946 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2184 2193
Size: 54 1425 50 275 18 180 174 9
Cuts at: 2193 2377 3036 3192 3264 3321 3775 4664 6293
Size: 184 659 156 72 57 454 889 1629
Cuts at: 6293 8
Size: 43
Fragments arranged by size:
1629 1425 889 659 454 275 184 180
174 156 72 57 54 50 43 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 2377 3775 1537
Size: 275 565 1398 4090
Fragments arranged by size:
4090 1398 565 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 2990 3061 3185 3503 4258 1353
Size: 1272 365 71 124 318 755 3423
Fragments arranged by size:
3423 1272 755 365 318 124 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2063 2470 3055 4915 5289 5435
Size: 1064 138 474 407 585 1860 374 146
Cuts at: 5435 387
Size: 1280
Fragments arranged by size:
1860 1280 1064 585 474 407 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2775 3324 4391 4988 5775 6043 1239
Size: 393 1143 549 1067 597 787 268 1524
Fragments arranged by size:
1524 1143 1067 787 597 549 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3303 3303
Size: 6328
EcoO109I rG'GnC_Cy
Cuts at: 2068 2750 3051 3581 2068
Size: 682 301 530 4815
Fragments arranged by size:
4815 682 530 301
EcoRI G'AATT_C
Cuts at: 3637 4015 3637
Size: 378 5950
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 2078 2207 2260 2268 2352 2391 2399 2438
Size: 400 129 53 8 84 39 8 39
Cuts at: 2438 2456 2475 2738 3153 5475 1678
Size: 18 19 263 415 2322 2531
Fragments arranged by size:
2531 2322 415 400 263 129 84 53
39 39 19 18 8 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3347 3379 3602 3634 4969
Size: 32 135 32 1775 32 223 32 1335
Cuts at: 4969 5001 5043 5075 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1775 1335 223 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3160 3343 3355 3394 3672 3918
Size: 988 281 1222 183 12 39 278 246
Cuts at: 3918 669
Size: 3079
Fragments arranged by size:
3079 1222 988 281 278 246 183 39
12
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KasI G'GCGC_C
Cuts at: 2348 2387 2471 3149 2348
Size: 39 84 678 5527
Fragments arranged by size:
5527 678 84 39
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 5442 5626 1305
Size: 1305 2832 184 2007
Fragments arranged by size:
2832 2007 1305 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3038 3323 10
Size: 54 1425 343 1206 285 3015
Fragments arranged by size:
3015 1425 1206 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4286 4645 4804 518
Size: 648 3120 359 159 2042
Fragments arranged by size:
3120 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2145 2323 2510 3034 3079 3290 3532
Size: 439 100 178 187 524 45 211 242
Cuts at: 3532 3744 4421 5569 5814 1606
Size: 212 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 439 245 242 212
211 187 178 100 45
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 6328
NarI GG'CG_CC
Cuts at: 2349 2388 2472 3150 2349
Size: 39 84 678 5527
Fragments arranged by size:
5527 678 84 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 2802 2950 513
Size: 552 939 798 148 3891
Fragments arranged by size:
3891 939 798 552 148
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 3686 5231 1132
Size: 97 2457 1545 2229
Fragments arranged by size:
2457 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3190 1108
Size: 2082 4246
PfoI T'CCnGG_A
Cuts at: 2556 2816 2556
Size: 260 6068
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3051 3051
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PspOMI G'GGCC_C
Cuts at: 2248 3581 2248
Size: 1333 4995
PstI C_TGCA'G
Cuts at: 830 2466 3680 830
Size: 1636 1214 3478
Fragments arranged by size:
3478 1636 1214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
SacII CC_GC'GG
Cuts at: 2511 2511
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3353 3392 3670 1936
Size: 1417 39 278 4594
Fragments arranged by size:
4594 1417 278 39
SapI GCTCTTCn'nnn_
Cuts at: 3185 3185
Size: 6328
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SexAI A'CCwGG_T
Cuts at: 2133 2589 2133
Size: 456 5872
SfcI C'TryA_G
Cuts at: 826 1045 2462 3676 4811 5492 5683 826
Size: 219 1417 1214 1135 681 191 1471
Fragments arranged by size:
1471 1417 1214 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2192 2192
Size: 6328
SfoI GGC'GCC
Cuts at: 2350 2389 2473 3151 2350
Size: 39 84 678 5527
Fragments arranged by size:
5527 678 84 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 2129 2280 3655 1872
Size: 257 151 1375 4545
Fragments arranged by size:
4545 1375 257 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2022 2260 4432 5333 5595 820
Size: 197 926 79 238 2172 901 262 1553
Fragments arranged by size:
2172 1553 926 901 262 238 197 79
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3686 1132
Size: 2554 3774
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6328
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2095 2802 2908 2950 3228
Size: 552 939 27 64 707 106 42 278
Cuts at: 3228 3767 513
Size: 539 3074
Fragments arranged by size:
3074 939 707 552 539 278 106 64
42 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4355 4372 4525 4710 5155 325
Size: 1255 2775 17 153 185 445 1498
Fragments arranged by size:
2775 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2916 4574 6286
Size: 274 80 33 51 1443 939 1658 1712
Cuts at: 6286 96
Size: 138
Fragments arranged by size:
1712 1658 1443 939 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 2640 2672 6288 6320 2202
Size: 32 406 32 3616 32 2210
Fragments arranged by size:
3616 2210 406 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2806 3160 2068
Size: 738 354 5236
Fragments arranged by size:
5236 738 354
XhoI C'TCGA_G
Cuts at: 1943 2022 1943
Size: 79 6249
XmaI C'CCGG_G
Cuts at: 1870 2127 2278 3653 1870
Size: 257 151 1375 4545
Fragments arranged by size:
4545 1375 257 151
XmnI GAAnn'nnTTC
Cuts at: 4457 4457
Size: 6328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SanDI SrfI SwaI