Restriction map for
pFN21AB5080
(Circular) (Six-base) MAPSORT of: pFN21AB5080.seq Check: 1988 from: 1 to: 6880
pFN21AB5080 6880 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2754 3650 4223 1937
Size: 817 896 573 4594
Fragments arranged by size:
4594 896 817 573
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 2913 3572 4943 6327 6595 1632
Size: 965 316 659 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 965 659 316 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6683
AflIII A'CryG_T
Cuts at: 1203 5779 6756 1203
Size: 4576 977 1327
Fragments arranged by size:
4576 1327 977
AgeI A'CCGG_T
Cuts at: 2055 5711 2055
Size: 3656 3224
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2213 3159 1184
Size: 1029 946 4905
Fragments arranged by size:
4905 1029 946
AlwNI CAG_nnn'CTG
Cuts at: 2575 2879 2963 3062 4623 6195 2575
Size: 304 84 99 1561 1572 3260
Fragments arranged by size:
3260 1572 1561 304 99 84
ApaLI G'TGCA_C
Cuts at: 3212 3455 4937 6093 3212
Size: 243 1482 1156 3999
Fragments arranged by size:
3999 1482 1156 243
ApoI r'AATT_y
Cuts at: 1493 2257 2414 2672 4189 4415 4567 6676 1493
Size: 764 157 258 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 764 258 226 157 152
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 3228 4205 1870
Size: 73 128 1157 977 4545
Fragments arranged by size:
4545 1157 977 128 73
BamHI G'GATC_C
Cuts at: 4210 4577 4210
Size: 367 6513
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4201 5656 618
Size: 325 217 627 2414 1455 1842
Fragments arranged by size:
2414 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3929 4199 1391
Size: 2538 270 4072
Fragments arranged by size:
4072 2538 270
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3640 928
Size: 971 1741 4168
Fragments arranged by size:
4168 1741 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4772 5982 4772
Size: 1210 5670
BclI T'GATC_A
Cuts at: 1548 2791 3051 3598 1548
Size: 1243 260 547 4830
Fragments arranged by size:
4830 1243 547 260
BfrBI ATG'CAT
Cuts at: 2266 2266
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5491 136
Size: 107 122 71 631 605 337 3482 1525
Fragments arranged by size:
3482 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6875 6875
Size: 6880
BlpI GC'TnA_GC
Cuts at: 4297 4297
Size: 6880
Bme1580I G_kGCm'C
Cuts at: 1790 3216 3459 4941 6097 1790
Size: 1426 243 1482 1156 2573
Fragments arranged by size:
2573 1482 1426 1156 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 4611 5569
Size: 416 53 83 399 24 808 2386 958
Cuts at: 5569 442
Size: 1753
Fragments arranged by size:
2386 1753 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4040
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2544 3279 4017 5540 1239
Size: 1305 735 738 1523 2579
Fragments arranged by size:
2579 1523 1305 738 735
Bpu10I CC'TnA_GC
Cuts at: 3952 4113 3952
Size: 161 6719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3539 5005 5870 6168 3539
Size: 1466 865 298 4251
Fragments arranged by size:
4251 1466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3551 5543 882
Size: 617 2052 1992 2219
Fragments arranged by size:
2219 2052 1992 617
BsaAI yAC'GTr
Cuts at: 493 1204 4671 493
Size: 711 3467 2702
Fragments arranged by size:
3467 2702 711
BsaBI GATnn'nnATC
Cuts at: 6874 6874
Size: 6880
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4687 5069 5704 275
Size: 53 83 186 936 3154 382 635 1451
Fragments arranged by size:
3154 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 5313 5711 5985 6132 6543 2055
Size: 3258 398 274 147 411 2392
Fragments arranged by size:
3258 2392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2517 2547 3264 3294 3990
Size: 30 165 30 1114 30 717 30 696
Cuts at: 3990 4020 1178
Size: 30 4038
Fragments arranged by size:
4038 1114 717 696 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 2451 2649 2879 3216 4163 1186
Size: 1004 261 198 230 337 947 3903
Fragments arranged by size:
3903 1004 947 337 261 230 198
BseYI C'CCAG_C
Cuts at: 2313 2872 2908 3446 6083 6579 2313
Size: 559 36 538 2637 496 2614
Fragments arranged by size:
2637 2614 559 538 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2352 2858 3772 1163
Size: 573 616 506 914 4271
Fragments arranged by size:
4271 914 616 573 506
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2498 3216 3459 3929 4114 4941 5026 6097 2498
Size: 718 243 470 185 827 85 1071 3281
Fragments arranged by size:
3281 1071 827 718 470 243 185 85
BsmI GAATG_Cn'
Cuts at: 4391 4484 4391
Size: 93 6787
BsmBI CGTCTCn'nnnn_
Cuts at: 2072 3267 2072
Size: 1195 5685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2498 3216 3459 3929 4114 4199 4941
Size: 399 708 718 243 470 185 85 742
Cuts at: 4941 5026 6097 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 718 708 470 399 243
185 85 85
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 2610 4769 6499 2610
Size: 2159 1730 2991
Fragments arranged by size:
2991 2159 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4235 844
Size: 3391 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2306 4767 1394
Size: 429 483 2461 3507
Fragments arranged by size:
3507 2461 483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2163 2809 5375 5549 65
Size: 1183 915 646 2566 174 1396
Fragments arranged by size:
2566 1396 1183 915 646 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 5524 5711 1700
Size: 110 245 3469 187 2869
Fragments arranged by size:
3469 2869 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 2221 96
Size: 2125 4755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 4940 5952 4940
Size: 1012 5868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6880
BstBI TT'CG_AA
Cuts at: 3150 5684 3150
Size: 2534 4346
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6880
BstXI CCAn_nnnn'nTGG
Cuts at: 2215 2786 3761 4048 2215
Size: 571 975 287 5047
Fragments arranged by size:
5047 975 571 287
BstYI r'GATC_y
Cuts at: 1972 3172 4210 4577 4962 4979 6431 6517 6529
Size: 1200 1038 367 385 17 1452 86 12
Cuts at: 6529 6875 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1200 1038 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3651 3651
Size: 6880
BtgI C'CryG_G
Cuts at: 513 1065 2004 2797 513
Size: 552 939 793 4596
Fragments arranged by size:
4596 939 793 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6141
Fragments arranged by size:
6141 683 56
BtsI GCAGTG_nn'
Cuts at: 3631 4396 5190 5210 3631
Size: 765 794 20 5301
Fragments arranged by size:
5301 794 765 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3232 3267 3385 3420 529
Size: 35 2668 35 118 35 3989
Fragments arranged by size:
3989 2668 118 35 35 35
DraI TTT'AAA
Cuts at: 4184 4184
Size: 6880
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3899 5887 809
Size: 507 2583 1988 1802
Fragments arranged by size:
2583 1988 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 3427 4327
Size: 54 1425 50 275 18 522 1075 900
Cuts at: 4327 5216 6845 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1075 900 889 522 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3591 4810 1353
Size: 2238 1219 3423
Fragments arranged by size:
3423 2238 1219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 5467 5841 5987 387
Size: 1064 138 471 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 471 374 146 138
EcoICRI GAG'CTC
Cuts at: 3927 3927
Size: 6880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2544 2597 2913 3279 3572 4017 4943
Size: 393 912 53 316 366 293 445 926
Cuts at: 4943 5540 6327 6595 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 926 912 787 597 445 393 366
316 293 268 53
EcoNI CCTnn'n_nnAGG
Cuts at: 3198 3198
Size: 6880
EcoO109I rG'GnC_Cy
Cuts at: 2158 2456 2869 2158
Size: 298 413 6169
Fragments arranged by size:
6169 413 298
EcoRI G'AATT_C
Cuts at: 4189 4567 4189
Size: 378 6502
FspI TGC'GCA
Cuts at: 2201 5386 2201
Size: 3185 3695
FspAI rTGC'GCAy
Cuts at: 2201 2201
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 2879 6027 1678
Size: 1201 3148 2531
Fragments arranged by size:
3148 2531 1201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 2833 2865 3590
Size: 32 135 32 974 32 255 32 725
Cuts at: 3590 3622 3627 3659 4142 4174 5521 5553 5595
Size: 32 5 32 483 32 1347 32 42
Cuts at: 5595 5627 1373
Size: 32 2626
Fragments arranged by size:
2626 1347 974 725 483 255 135 42
32 32 32 32 32 32 32 32
32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4224 4470 669
Size: 988 281 2286 246 3079
Fragments arranged by size:
3079 2286 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6880
HpaI GTT'AAC
Cuts at: 4470 4470
Size: 6880
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 4479 4479
Size: 6880
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2932 3367 3482 5994 6178 1305
Size: 1627 435 115 2512 184 2007
Fragments arranged by size:
2512 2007 1627 435 184 115
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2354 3429 10
Size: 54 1425 343 522 1075 3461
Fragments arranged by size:
3461 1425 1075 522 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2101 2348 2802 3008 3194 4031 4046
Size: 648 935 247 454 206 186 837 15
Cuts at: 4046 4838 5197 5356 518
Size: 792 359 159 2042
Fragments arranged by size:
2042 935 837 792 648 454 359 247
206 186 159 15
MspA1I CmG'CkG
Cuts at: 1606 4296 4973 6121 6366 1606
Size: 2690 677 1148 245 2120
Fragments arranged by size:
2690 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2797 513
Size: 552 939 793 4596
Fragments arranged by size:
4596 939 793 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6880
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NsiI A_TGCA'T
Cuts at: 2268 2268
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 2270 2771 3733 4238 5783 1132
Size: 97 1041 501 962 505 1545 2229
Fragments arranged by size:
2229 1545 1041 962 505 501 97
PasI CC'CwG_GG
Cuts at: 1348 3541 1348
Size: 2193 4687
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2879 4076 1108
Size: 1771 1197 3912
Fragments arranged by size:
3912 1771 1197
PfoI T'CCnGG_A
Cuts at: 2383 3843 2383
Size: 1460 5420
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 6462 6494 4949
Size: 32 1481 32 5335
Fragments arranged by size:
5335 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2158
Size: 6880
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PspXI vC'TCGA_Gb
Cuts at: 3228 3228
Size: 6880
PstI C_TGCA'G
Cuts at: 830 2192 2923 3138 3514 4232 830
Size: 1362 731 215 376 718 3478
Fragments arranged by size:
3478 1362 731 718 376 215
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
SacI G_AGCT'C
Cuts at: 3929 3929
Size: 6880
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SbfI CC_TGCA'GG
Cuts at: 2192 4232 2192
Size: 2040 4840
ScaI AGT'ACT
Cuts at: 5128 6840 5128
Size: 1712 5168
SfcI C'TryA_G
Cuts at: 826 1045 2188 2830 2919 3134 3510 4099 4228
Size: 219 1143 642 89 215 376 589 129
Cuts at: 4228 5363 6044 6235 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 681 642 589 376 219
215 191 129 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 4207 1872
Size: 2335 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3228 3554 4984 5885 6147 820
Size: 197 926 1285 326 1430 901 262 1553
Fragments arranged by size:
1553 1430 1285 926 901 326 262 197
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 3733 4238 1132
Size: 2601 505 3774
Fragments arranged by size:
3774 2601 505
SspI AAT'ATT
Cuts at: 5 52 3382 4804 5
Size: 47 3330 1422 2081
Fragments arranged by size:
3330 2081 1422 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6880
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 2797 3760 3870 4319 513
Size: 552 939 150 643 963 110 449 3074
Fragments arranged by size:
3074 963 939 643 552 449 150 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 2207 3447 3587 4907 4924 5077
Size: 1255 509 118 1240 140 1320 17 153
Cuts at: 5077 5262 5707 325
Size: 185 445 1498
Fragments arranged by size:
1498 1320 1255 1240 509 445 185 153
140 118 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2221 2811
Size: 274 80 33 51 1443 147 97 590
Cuts at: 2811 3375 5126 6838 96
Size: 564 1751 1712 138
Fragments arranged by size:
1751 1712 1443 590 564 274 147 138
97 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2361 2393 3990 4022 6840 6872 2361
Size: 32 1597 32 2818 32 2369
Fragments arranged by size:
2818 2369 1597 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6880
XbaI T'CTAG_A
Cuts at: 4216 4216
Size: 6880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2509 2509
Size: 6880
XhoI C'TCGA_G
Cuts at: 1943 3228 1943
Size: 1285 5595
XmaI C'CCGG_G
Cuts at: 1870 4205 1870
Size: 2335 4545
XmnI GAAnn'nnTTC
Cuts at: 2717 4094 5009 2717
Size: 1377 915 4588
Fragments arranged by size:
4588 1377 915
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI Bsu36I DraIII EcoRV FalI FseI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI