Restriction map for
pFN21AB5068
(Circular) (Six-base) MAPSORT of: pFN21AB5068.seq Check: 1271 from: 1 to: 6346
pFN21AB5068 6346 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3023 3023
Size: 6346
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3689 1937
Size: 1752 4594
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2045 2388 2976 4409 5793 6061 1632
Size: 405 8 343 588 1433 1384 268 1917
Fragments arranged by size:
1917 1433 1384 588 405 343 268 8
AflII C'TTAA_G
Cuts at: 820 1017 2657 3383 820
Size: 197 1640 726 3783
Fragments arranged by size:
3783 1640 726 197
AflIII A'CryG_T
Cuts at: 1203 5245 6222 1203
Size: 4042 977 1327
Fragments arranged by size:
4042 1327 977
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 2861 1166
Size: 1695 4651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 1184
Size: 1620 4726
AlwNI CAG_nnn'CTG
Cuts at: 2317 2366 3424 4089 5661 2317
Size: 49 1058 665 1572 3002
Fragments arranged by size:
3002 1572 1058 665 49
ApaI G_GGCC'C
Cuts at: 2158 2971 3003 3236 2158
Size: 813 32 233 5268
Fragments arranged by size:
5268 813 233 32
ApaLI G'TGCA_C
Cuts at: 4403 5559 4403
Size: 1156 5190
ApoI r'AATT_y
Cuts at: 1493 2735 3620 3655 3881 4033 6142 1493
Size: 1242 885 35 226 152 2109 1697
Fragments arranged by size:
2109 1697 1242 885 226 152 35
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2334 3042 3671 1870
Size: 73 208 183 708 629 4545
Fragments arranged by size:
4545 708 629 208 183 73
AvrII C'CTAG_G
Cuts at: 3407 3407
Size: 6346
BamHI G'GATC_C
Cuts at: 3676 4043 3676
Size: 367 5979
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2895 3667 5122 618
Size: 325 217 627 1108 772 1455 1842
Fragments arranged by size:
1842 1455 1108 772 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2158 2168 2544 2971 3003 3236 3665 1391
Size: 767 10 376 427 32 233 429 4072
Fragments arranged by size:
4072 767 429 427 376 233 32 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2180 2376 2736 3405 3540 928
Size: 971 281 196 360 669 135 3734
Fragments arranged by size:
3734 971 669 360 281 196 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3311 3345 4537 4571 1903
Size: 34 1374 34 1192 34 3678
Fragments arranged by size:
3678 1374 1192 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4957 136
Size: 107 122 71 631 605 3285 1525
Fragments arranged by size:
3285 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 3763 3763
Size: 6346
Bme1580I G_kGCm'C
Cuts at: 1790 2158 2971 3003 3236 4407 5563 1790
Size: 368 813 32 233 1171 1156 2573
Fragments arranged by size:
2573 1171 1156 813 368 233 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2033 2966 4077
Size: 416 53 83 399 24 616 933 1111
Cuts at: 4077 5035 442
Size: 958 1753
Fragments arranged by size:
1753 1111 958 933 616 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2504 2875 3401 5006 1239
Size: 1265 371 526 1605 2579
Fragments arranged by size:
2579 1605 1265 526 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2609 2744 4471 5336 5634 2528
Size: 81 135 1727 865 298 3240
Fragments arranged by size:
3240 1727 865 298 135 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4137 493
Size: 711 2933 2702
Fragments arranged by size:
2933 2702 711
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4153 4535 5170 275
Size: 53 83 186 936 2620 382 635 1451
Fragments arranged by size:
2620 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2233 3025 3258 4779 5177 5451 5598 6009 2233
Size: 792 233 1521 398 274 147 411 2570
Fragments arranged by size:
2570 1521 792 411 398 274 233 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6121
Fragments arranged by size:
6121 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 3221 3281 1186
Size: 1357 678 60 4251
Fragments arranged by size:
4251 1357 678 60
BseYI C'CCAG_C
Cuts at: 2673 5549 6045 2673
Size: 2876 496 2974
Fragments arranged by size:
2974 2876 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5773
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 3796 4557 5585 1540
Size: 275 186 180 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 2335 2544 2868 3078 4407 4492 5563 2335
Size: 209 324 210 1329 85 1071 3118
Fragments arranged by size:
3118 1329 1071 324 210 209 85
BsmI GAATG_Cn'
Cuts at: 3370 3857 3950 3370
Size: 487 93 5766
Fragments arranged by size:
5766 487 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2168 2335 2544 2868 2971 3003
Size: 399 368 10 167 209 324 103 32
Cuts at: 3003 3078 3236 3665 4407 4492 5563 1391
Size: 75 158 429 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 368 324 209
167 158 103 85 75 32 10
BspEI T'CCGG_A
Cuts at: 3025 3258 6009 3025
Size: 233 2751 3362
Fragments arranged by size:
3362 2751 233
BspHI T'CATG_A
Cuts at: 4235 5965 4235
Size: 1730 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3023 3701 844
Size: 2179 678 3489
Fragments arranged by size:
3489 2179 678
BsrBI CCG'CTC
Cuts at: 1394 1823 4233 1394
Size: 429 2410 3507
Fragments arranged by size:
3507 2410 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4841 5015 65
Size: 1183 3593 174 1396
Fragments arranged by size:
3593 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2820 4990 5177 1700
Size: 110 1010 2170 187 2869
Fragments arranged by size:
2869 2170 1010 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 4406 5418 4406
Size: 1012 5334
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2209 2951 794
Size: 1415 742 4189
Fragments arranged by size:
4189 1415 742
BstBI TT'CG_AA
Cuts at: 2031 5150 2031
Size: 3119 3227
BstEII G'GTnAC_C
Cuts at: 1184 2650 1184
Size: 1466 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2284 2681 3122 2122
Size: 162 397 441 5346
Fragments arranged by size:
5346 441 397 162
BstYI r'GATC_y
Cuts at: 1972 3676 4043 4428 4445 5897 5983 5995 6341
Size: 1704 367 385 17 1452 86 12 346
Cuts at: 6341 1972
Size: 1977
Fragments arranged by size:
1977 1704 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3102 3102
Size: 6346
BtgI C'CryG_G
Cuts at: 513 1065 2004 3177 3508 513
Size: 552 939 1173 331 3351
Fragments arranged by size:
3351 1173 939 552 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 2882 3459 3590 3862 4656 4676 2256
Size: 626 577 131 272 794 20 3926
Fragments arranged by size:
3926 794 626 577 272 131 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 3650 3650
Size: 6346
DraIII CAC_nnn'GTG
Cuts at: 3064 3064
Size: 6346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5353 809
Size: 507 4037 1802
Fragments arranged by size:
4037 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2349 3793
Size: 54 1425 50 275 18 348 171 1444
Cuts at: 3793 4682 6311 8
Size: 889 1629 43
Fragments arranged by size:
1629 1444 1425 889 348 275 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 3793 1537
Size: 275 366 1615 4090
Fragments arranged by size:
4090 1615 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2532 3354 4276 1353
Size: 1179 822 922 3423
Fragments arranged by size:
3423 1179 922 822
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4933 5307 5453 387
Size: 1064 138 3344 374 146 1280
Fragments arranged by size:
3344 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 2542
Size: 6346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2045 2388 2504 2875 2976 3401
Size: 393 405 8 343 116 371 101 425
Cuts at: 3401 4409 5006 5793 6061 1239
Size: 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 787 597 425 405 393 371
343 268 116 101 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3556 3556
Size: 6346
EcoO109I rG'GnC_Cy
Cuts at: 2140 2424 2967 2968 2999 3232 3233 3412 2140
Size: 284 543 1 31 233 1 179 5074
Fragments arranged by size:
5074 543 284 233 179 31 1 1
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2528 3409 2528
Size: 881 5465
FspI TGC'GCA
Cuts at: 3596 4852 3596
Size: 1256 5090
HaeII r_GCGC'y
Cuts at: 1678 5493 1678
Size: 3815 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2416 2448 3002
Size: 32 135 32 791 32 21 32 554
Cuts at: 3002 3034 4987 5019 5061 5093 1373
Size: 32 1953 32 42 32 2626
Fragments arranged by size:
2626 1953 791 554 135 42 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2800 3690 3936 669
Size: 988 281 862 890 246 3079
Fragments arranged by size:
3079 988 890 862 281 246
HindIII A'AGCT_T
Cuts at: 748 3380 748
Size: 2632 3714
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5460 5644 1305
Size: 4155 184 2007
Fragments arranged by size:
4155 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 10
Size: 54 1425 343 519 4005
Fragments arranged by size:
4005 1425 519 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2861 4304 4663 4822 518
Size: 648 1695 1443 359 159 2042
Fragments arranged by size:
2042 1695 1443 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2317 2363 2677 3421 3762 4439 5587
Size: 600 111 46 314 744 341 677 1148
Cuts at: 5587 5832 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 744 677 600 341 314 245
111 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4855
Fragments arranged by size:
4855 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6346
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NruI TCG'CGA
Cuts at: 2089 2089
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 3704 5249 1132
Size: 97 2475 1545 2229
Fragments arranged by size:
2475 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 5245 5245
Size: 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6346
PfoI T'CCnGG_A
Cuts at: 2103 2168 2103
Size: 65 6281
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 2279 4415 4447 5928 5960 2247
Size: 32 2136 32 1481 32 2633
Fragments arranged by size:
2633 2136 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2424 3412 2140
Size: 284 988 5074
Fragments arranged by size:
5074 988 284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 3916 3916
Size: 6346
PspOMI G'GGCC_C
Cuts at: 2154 2967 2999 3232 2154
Size: 813 32 233 5268
Fragments arranged by size:
5268 813 233 32
PstI C_TGCA'G
Cuts at: 830 2205 3296 3698 830
Size: 1375 1091 402 3478
Fragments arranged by size:
3478 1375 1091 402
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 2206 2317 2363 2677 3421 2206
Size: 111 46 314 744 5131
Fragments arranged by size:
5131 744 314 111 46
SacI G_AGCT'C
Cuts at: 2544 2544
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 3688 1936
Size: 1752 4594
SanDI GG'GwC_CC
Cuts at: 2424 2424
Size: 6346
SapI GCTCTTCn'nnn_
Cuts at: 2532 3354 2532
Size: 822 5524
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 4594 6306 4594
Size: 1712 4634
SexAI A'CCwGG_T
Cuts at: 3489 3489
Size: 6346
SfcI C'TryA_G
Cuts at: 826 1045 2201 3292 3694 4829 5510 5701 826
Size: 219 1156 1091 402 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 1091 681 402 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 3044 3673 1872
Size: 1172 629 4545
Fragments arranged by size:
4545 1172 629
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 2624 2657 2759 3383 4450
Size: 197 926 600 81 33 102 624 1067
Cuts at: 4450 5351 5613 820
Size: 901 262 1553
Fragments arranged by size:
1553 1067 926 901 624 600 262 197
102 81 33
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3704 1132
Size: 2572 3774
SspI AAT'ATT
Cuts at: 5 52 4270 5
Size: 47 4218 2081
Fragments arranged by size:
4218 2081 47
StuI AGG'CCT
Cuts at: 1517 2259 3017 3107 1517
Size: 742 758 90 4756
Fragments arranged by size:
4756 758 742 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 2920 3407 3785 513
Size: 552 939 273 643 487 378 3074
Fragments arranged by size:
3074 939 643 552 487 378 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4373 4390 4543 4728 5173 325
Size: 1255 2793 17 153 185 445 1498
Fragments arranged by size:
2793 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4592 6304 96
Size: 274 80 33 51 1443 2615 1712 138
Fragments arranged by size:
2615 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6306 6338 6306
Size: 32 6314
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 2936 1316
Size: 1059 561 4726
Fragments arranged by size:
4726 1059 561
XbaI T'CTAG_A
Cuts at: 2806 3682 2806
Size: 876 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 3042 3671 1870
Size: 1172 629 4545
Fragments arranged by size:
4545 1172 629
XmnI GAAnn'nnTTC
Cuts at: 3363 4475 3363
Size: 1112 5234
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI
RsrII SacII SfiI SfoI SrfI SwaI XcmI