Restriction map for
pFN21AB5063
(Circular) (Six-base) MAPSORT of: pFN21AB5063.seq Check: 6395 from: 1 to: 7030
pFN21AB5063 7030 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3432 4840 278
Size: 53 83 186 936 1896 1408 2468
Fragments arranged by size:
2468 1896 1408 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 4373 1937
Size: 1332 1104 4594
Fragments arranged by size:
4594 1332 1104
Acc65I G'GTAC_C
Cuts at: 4351 4351
Size: 7030
AclI AA'CG_TT
Cuts at: 1563 2425 5157 5530 1563
Size: 862 2732 373 3063
Fragments arranged by size:
3063 2732 862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 4128 4328 5093 6477 6745 1632
Size: 863 1633 200 765 1384 268 1917
Fragments arranged by size:
1917 1633 1384 863 765 268 200
AfeI AGC'GCT
Cuts at: 2269 2269
Size: 7030
AflII C'TTAA_G
Cuts at: 820 1017 2690 820
Size: 197 1673 5160
Fragments arranged by size:
5160 1673 197
AflIII A'CryG_T
Cuts at: 1203 4165 5929 6906 1203
Size: 2962 1764 977 1327
Fragments arranged by size:
2962 1764 1327 977
AgeI A'CCGG_T
Cuts at: 5861 5861
Size: 7030
AhdI GACnn_n'nnGTC
Cuts at: 2023 5759 2023
Size: 3736 3294
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7030
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 2927 2959 2552
Size: 32 343 32 6623
Fragments arranged by size:
6623 343 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2134 2416 2656 2773 4013 4773 6345 2134
Size: 282 240 117 1240 760 1572 2819
Fragments arranged by size:
2819 1572 1240 760 282 240 117
ApaI G_GGCC'C
Cuts at: 2540 3965 2540
Size: 1425 5605
ApaLI G'TGCA_C
Cuts at: 5087 6243 5087
Size: 1156 5874
ApoI r'AATT_y
Cuts at: 1493 3380 4339 4565 4717 6826 1493
Size: 1887 959 226 152 2109 1697
Fragments arranged by size:
2109 1887 1697 959 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5584 160
Size: 872 4552 1606
Fragments arranged by size:
4552 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7030
AvaI C'yCGr_G
Cuts at: 1870 1943 2463 3615 4355 1870
Size: 73 520 1152 740 4545
Fragments arranged by size:
4545 1152 740 520 73
BamHI G'GATC_C
Cuts at: 2163 4258 4360 4727 2163
Size: 2095 102 367 4466
Fragments arranged by size:
4466 2095 367 102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2400 3778 4123 4351 5806
Size: 325 217 627 613 1378 345 228 1455
Cuts at: 5806 618
Size: 1842
Fragments arranged by size:
1842 1455 1378 627 613 345 325 228
217
BanII G_rGCy'C
Cuts at: 1391 2259 2540 3051 3099 3965 4349 1391
Size: 868 281 511 48 866 384 4072
Fragments arranged by size:
4072 868 866 511 384 281 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3832 4320 928
Size: 971 1933 488 3638
Fragments arranged by size:
3638 1933 971 488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5221 5255 1903
Size: 34 3284 34 3678
Fragments arranged by size:
3678 3284 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2191 4922 6132 2191
Size: 2731 1210 3089
Fragments arranged by size:
3089 2731 1210
BclI T'GATC_A
Cuts at: 1548 2496 1548
Size: 948 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5641 136
Size: 107 122 71 631 605 337 3632 1525
Fragments arranged by size:
3632 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 7025 7025
Size: 7030
BlpI GC'TnA_GC
Cuts at: 2124 2264 4447 2124
Size: 140 2183 4707
Fragments arranged by size:
4707 2183 140
Bme1580I G_kGCm'C
Cuts at: 1790 2540 3616 3783 3965 5091 6247 1790
Size: 750 1076 167 182 1126 1156 2573
Fragments arranged by size:
2573 1156 1126 1076 750 182 167
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7030
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3544 3960 4051
Size: 416 53 83 399 24 2127 416 91
Cuts at: 4051 4761 5719 442
Size: 710 958 1753
Fragments arranged by size:
2127 1753 958 710 416 416 399 91
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7030
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3113
Size: 7030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2795 3148 3488 3740 4007 4035 5690
Size: 1340 216 353 340 252 267 28 1655
Cuts at: 5690 1239
Size: 2579
Fragments arranged by size:
2579 1655 1340 353 340 267 252 216
28
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 7030
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2060 2297 5155 6020 6318 2060
Size: 237 2858 865 298 2772
Fragments arranged by size:
2858 2772 865 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3126 3794 5693 882
Size: 617 1627 668 1899 2219
Fragments arranged by size:
2219 1899 1627 668 617
BsaAI yAC'GTr
Cuts at: 493 1204 2798 4168 4821 493
Size: 711 1594 1370 653 2702
Fragments arranged by size:
2702 1594 1370 711 653
BsaBI GATnn'nnATC
Cuts at: 7024 7024
Size: 7030
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2206 3429 4837 5219
Size: 53 83 186 936 673 1223 1408 382
Cuts at: 5219 5854 275
Size: 635 1451
Fragments arranged by size:
1451 1408 1223 936 673 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2604 5463 5861 6135 6282 6693 2604
Size: 2859 398 274 147 411 2941
Fragments arranged by size:
2941 2859 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 3121 3151 3615
Size: 30 165 30 1149 30 539 30 464
Cuts at: 3615 3645 1178
Size: 30 4563
Fragments arranged by size:
4563 1149 539 464 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 3102 3107 3161 3881 1186
Size: 1201 715 5 54 720 4335
Fragments arranged by size:
4335 1201 720 715 54 5
BseYI C'CCAG_C
Cuts at: 2259 2409 3790 6233 6729 2259
Size: 150 1381 2443 496 2560
Fragments arranged by size:
2560 2443 1381 496 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3355 3455 3464 1163
Size: 573 1619 100 9 4729
Fragments arranged by size:
4729 1619 573 100 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4480 5241 6269 1540
Size: 275 186 2479 761 1028 2301
Fragments arranged by size:
2479 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3051 3099 3354 5091 5176 6247 3051
Size: 48 255 1737 85 1071 3834
Fragments arranged by size:
3834 1737 1071 255 85 48
BsmI GAATG_Cn'
Cuts at: 4541 4634 4541
Size: 93 6937
BsmBI CGTCTCn'nnnn_
Cuts at: 2063 2213 2063
Size: 150 6880
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2540 3051 3099 3354 3616 3783
Size: 399 469 281 511 48 255 262 167
Cuts at: 3783 3965 4349 5091 5176 6247 1391
Size: 182 384 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 511 469 399 384 281
262 255 182 167 85 48
BspEI T'CCGG_A
Cuts at: 6693 6693
Size: 7030
BspHI T'CATG_A
Cuts at: 4919 6649 4919
Size: 1730 5300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2509 2782 3477 4004 4385 844
Size: 1665 273 695 527 381 3489
Fragments arranged by size:
3489 1665 695 527 381 273
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 4917 1394
Size: 429 506 2588 3507
Fragments arranged by size:
3507 2588 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3177 3424 5525 5699 65
Size: 1183 1929 247 2101 174 1396
Fragments arranged by size:
2101 1929 1396 1183 247 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2403 5674 5861 1700
Size: 110 593 3271 187 2869
Fragments arranged by size:
3271 2869 593 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7030
BssHII G'CGCG_C
Cuts at: 1925 2094 1925
Size: 169 6861
BssSI C'ACGA_G
Cuts at: 3702 5090 6102 3702
Size: 1388 1012 4630
Fragments arranged by size:
4630 1388 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3521 794
Size: 2727 4303
BstBI TT'CG_AA
Cuts at: 5834 5834
Size: 7030
BstEII G'GTnAC_C
Cuts at: 1184 3991 1184
Size: 2807 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 2705 3766 2705
Size: 1061 5969
BstYI r'GATC_y
Cuts at: 1972 2163 2244 3498 4111 4258 4360 4727 5112
Size: 191 81 1254 613 147 102 367 385
Cuts at: 5112 5129 6581 6667 6679 7025 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1254 613 385 367 346 191
147 102 86 81 17 12
BstZ17I GTA'TAC
Cuts at: 3270 3270
Size: 7030
Bsu36I CC'TnA_GG
Cuts at: 3591 3917 3591
Size: 326 6704
BtgI C'CryG_G
Cuts at: 513 1065 2004 3141 3545 3708 4254 513
Size: 552 939 1137 404 163 546 3289
Fragments arranged by size:
3289 1137 939 552 546 404 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6291
Fragments arranged by size:
6291 683 56
BtsI GCAGTG_nn'
Cuts at: 3152 3328 3452 4546 5340 5360 3152
Size: 176 124 1094 794 20 4822
Fragments arranged by size:
4822 1094 794 176 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7030
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2604 2639 529
Size: 35 2040 35 4920
Fragments arranged by size:
4920 2040 35 35
DraI TTT'AAA
Cuts at: 4334 4334
Size: 7030
DraIII CAC_nnn'GTG
Cuts at: 4045 4171 4045
Size: 126 6904
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2519 6037 809
Size: 507 1203 3518 1802
Fragments arranged by size:
3518 1802 1203 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2952 3457 4477
Size: 54 1425 50 275 18 1122 505 1020
Cuts at: 4477 5366 6995 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1122 1020 889 505 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4477 1537
Size: 275 2665 4090
Fragments arranged by size:
4090 2665 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 3403 4960 1353
Size: 683 1367 1557 3423
Fragments arranged by size:
3423 1557 1367 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5617 5991 6137 387
Size: 1064 138 4028 374 146 1280
Fragments arranged by size:
4028 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3049 3097 3049
Size: 48 6982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 2579 2795 3148 3488 3740 4007
Size: 393 863 84 216 353 340 252 267
Cuts at: 4007 4035 4128 4328 5093 5690 6477 6745 1239
Size: 28 93 200 765 597 787 268 1524
Fragments arranged by size:
1524 863 787 765 597 393 353 340
268 267 252 216 200 93 84 28
EcoO109I rG'GnC_Cy
Cuts at: 2521 2537 2686 3961 3962 2521
Size: 16 149 1275 1 5589
Fragments arranged by size:
5589 1275 149 16 1
EcoRI G'AATT_C
Cuts at: 3380 4339 4717 3380
Size: 959 378 5693
Fragments arranged by size:
5693 959 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4303 4303
Size: 7030
FspI TGC'GCA
Cuts at: 3176 5536 3176
Size: 2360 4670
HaeII r_GCGC'y
Cuts at: 1678 2271 3892 6177 1678
Size: 593 1621 2285 2531
Fragments arranged by size:
2531 2285 1621 593
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2644 2676 3121
Size: 32 135 32 437 32 603 32 445
Cuts at: 3121 3153 5671 5703 5745 5777 1373
Size: 32 2518 32 42 32 2626
Fragments arranged by size:
2626 2518 603 445 437 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4374 4620 669
Size: 988 281 2436 246 3079
Fragments arranged by size:
3079 2436 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4280 748
Size: 3532 3498
HpaI GTT'AAC
Cuts at: 4620 4620
Size: 7030
KpnI G_GTAC'C
Cuts at: 4355 4355
Size: 7030
MfeI C'AATT_G
Cuts at: 4629 4629
Size: 7030
MluI A'CGCG_T
Cuts at: 6906 6906
Size: 7030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2526 6144 6328 1305
Size: 1221 3618 184 2007
Fragments arranged by size:
3618 2007 1221 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5208
Fragments arranged by size:
5208 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 3776 4988 5347 5506 518
Size: 648 2547 63 1212 359 159 2042
Fragments arranged by size:
2547 2042 1212 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 3794 4446 5123 6271 6516 1606
Size: 2188 652 677 1148 245 2120
Fragments arranged by size:
2188 2120 1148 677 652 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3141 3708 513
Size: 552 939 1137 567 3835
Fragments arranged by size:
3835 1137 939 567 552
NdeI CA'TA_TG
Cuts at: 387 3004 387
Size: 2617 4413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7030
NotI GC'GGCC_GC
Cuts at: 4477 4477
Size: 7030
NspI r_CATG'y
Cuts at: 1132 1229 2518 2846 3953 4388 5933 1132
Size: 97 1289 328 1107 435 1545 2229
Fragments arranged by size:
2229 1545 1289 1107 435 328 97
PasI CC'CwG_GG
Cuts at: 1348 2134 1348
Size: 786 6244
PciI A'CATG_T
Cuts at: 5929 5929
Size: 7030
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 2533 3910 1108
Size: 1308 117 1377 4228
Fragments arranged by size:
4228 1377 1308 117
PmeI GTTT'AAAC
Cuts at: 4334 4334
Size: 7030
PmlI CAC'GTG
Cuts at: 4168 4168
Size: 7030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 5099 5131 6612 6644 2552
Size: 32 2515 32 1481 32 2938
Fragments arranged by size:
2938 2515 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2521
Size: 7030
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7030
PsiI TTA'TAA
Cuts at: 4600 4600
Size: 7030
PspOMI G'GGCC_C
Cuts at: 2536 3961 2536
Size: 1425 5605
PstI C_TGCA'G
Cuts at: 830 3948 4001 4382 830
Size: 3118 53 381 3478
Fragments arranged by size:
3478 3118 381 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7030
PvuII CAG'CTG
Cuts at: 3794 3794
Size: 7030
SacI G_AGCT'C
Cuts at: 3051 3099 3051
Size: 48 6982
SalI G'TCGA_C
Cuts at: 1936 4372 1936
Size: 2436 4594
SapI GCTCTTCn'nnn_
Cuts at: 2036 3403 2036
Size: 1367 5663
SbfI CC_TGCA'GG
Cuts at: 4382 4382
Size: 7030
ScaI AGT'ACT
Cuts at: 5278 6990 5278
Size: 1712 5318
SfcI C'TryA_G
Cuts at: 826 1045 3249 3944 3997 4378 5513 6194 6385
Size: 219 2204 695 53 381 1135 681 191
Cuts at: 6385 826
Size: 1471
Fragments arranged by size:
2204 1471 1135 695 681 381 219 191
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7030
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7030
SmaI CCC'GGG
Cuts at: 1872 3617 4357 1872
Size: 1745 740 4545
Fragments arranged by size:
4545 1745 740
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2276 2690 5134 6035 6297
Size: 197 926 132 201 414 2444 901 262
Cuts at: 6297 820
Size: 1553
Fragments arranged by size:
2444 1553 926 901 414 262 201 197
132
SnaBI TAC'GTA
Cuts at: 493 2798 4821 493
Size: 2305 2023 2702
Fragments arranged by size:
2702 2305 2023
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7030
SphI G_CATG'C
Cuts at: 1132 4388 1132
Size: 3256 3774
SspI AAT'ATT
Cuts at: 5 52 4954 5
Size: 47 4902 2081
Fragments arranged by size:
4902 2081 47
StuI AGG'CCT
Cuts at: 1517 4031 1517
Size: 2514 4516
StyI C'CwwG_G
Cuts at: 513 1065 2004 2298 2877 3141 3375 3708 3765
Size: 552 939 294 579 264 234 333 57
Cuts at: 3765 4469 513
Size: 704 3074
Fragments arranged by size:
3074 939 704 579 552 333 294 264
234 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 3847 4033 5057 5074 5227 5412
Size: 1255 753 1514 186 1024 17 153 185
Cuts at: 5412 5857 325
Size: 445 1498
Fragments arranged by size:
1514 1498 1255 1024 753 445 186 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5276 6988 96
Size: 274 80 33 51 1443 3299 1712 138
Fragments arranged by size:
3299 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3615 3647 3733 3765 6990 7022 3284
Size: 32 299 32 86 32 3225 32 3292
Fragments arranged by size:
3292 3225 299 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2656 1316
Size: 1340 5690
XbaI T'CTAG_A
Cuts at: 2014 3661 4366 2014
Size: 1647 705 4678
Fragments arranged by size:
4678 1647 705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 2962 3715 2314
Size: 648 753 5629
Fragments arranged by size:
5629 753 648
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7030
XmaI C'CCGG_G
Cuts at: 1870 3615 4355 1870
Size: 1745 740 4545
Fragments arranged by size:
4545 1745 740
XmnI GAAnn'nnTTC
Cuts at: 2567 5159 2567
Size: 2592 4438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3430 4838 276
Size: 53 83 186 936 1896 1408 2468
Fragments arranged by size:
2468 1896 1408 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI