Restriction map for
pFN21AB5060
(Circular) (Six-base) MAPSORT of: pFN21AB5060.seq Check: 5432 from: 1 to: 6460
pFN21AB5060 6460 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3606 4270 278
Size: 53 83 186 936 2070 664 2468
Fragments arranged by size:
2468 2070 936 664 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3447 3803 1937
Size: 1510 356 4594
Fragments arranged by size:
4594 1510 356
Acc65I G'GTAC_C
Cuts at: 3781 3781
Size: 6460
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2990 4523 5907 6175 1632
Size: 719 639 1533 1384 268 1917
Fragments arranged by size:
1917 1533 1384 719 639 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6263
AflIII A'CryG_T
Cuts at: 1203 5359 6336 1203
Size: 4156 977 1327
Fragments arranged by size:
4156 1327 977
AgeI A'CCGG_T
Cuts at: 5291 5291
Size: 6460
AhdI GACnn_n'nnGTC
Cuts at: 2314 5189 2314
Size: 2875 3585
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6460
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 6428
AlwNI CAG_nnn'CTG
Cuts at: 2224 2557 2851 3145 3175 3270 4203 5775 2224
Size: 333 294 294 30 95 933 1572 2909
Fragments arranged by size:
2909 1572 933 333 294 294 95 30
ApaI G_GGCC'C
Cuts at: 2197 2197
Size: 6460
ApaLI G'TGCA_C
Cuts at: 2281 3276 4517 5673 2281
Size: 995 1241 1156 3068
Fragments arranged by size:
3068 1241 1156 995
ApoI r'AATT_y
Cuts at: 1493 3149 3769 3995 4147 6256 1493
Size: 1656 620 226 152 2109 1697
Fragments arranged by size:
2109 1697 1656 620 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5014 160
Size: 872 3982 1606
Fragments arranged by size:
3982 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 1870
Size: 73 1842 4545
Fragments arranged by size:
4545 1842 73
BamHI G'GATC_C
Cuts at: 3790 4157 3790
Size: 367 6093
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2947 3781 5236 618
Size: 325 217 627 306 854 834 1455 1842
Fragments arranged by size:
1842 1455 854 834 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2197 2421 3072 3507 3591 3779 1391
Size: 806 224 651 435 84 188 4072
Fragments arranged by size:
4072 806 651 435 224 188 84
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 6460
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3137 928
Size: 971 1238 4251
Fragments arranged by size:
4251 1238 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4651 4685 1903
Size: 34 2714 34 3678
Fragments arranged by size:
3678 2714 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3202 4352 5562 3202
Size: 1150 1210 4100
Fragments arranged by size:
4100 1210 1150
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2139 2219 2303
Size: 107 122 71 631 605 467 80 84
Cuts at: 2303 2457 2708 5071 136
Size: 154 251 2363 1525
Fragments arranged by size:
2363 1525 631 605 467 251 154 122
107 84 80 71
BglII A'GATC_T
Cuts at: 2118 2439 6455 2118
Size: 321 4016 2123
Fragments arranged by size:
4016 2123 321
BlpI GC'TnA_GC
Cuts at: 3877 3877
Size: 6460
Bme1580I G_kGCm'C
Cuts at: 1790 2144 2197 2285 2613 2952 3165 3280 3646
Size: 354 53 88 328 339 213 115 366
Cuts at: 3646 3699 4521 5677 1790
Size: 53 822 1156 2573
Fragments arranged by size:
2573 1156 822 366 354 339 328 213
115 88 53 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6460
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2889 3633 4191
Size: 416 53 83 399 24 1472 744 558
Cuts at: 4191 5149 442
Size: 958 1753
Fragments arranged by size:
1753 1472 958 744 558 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2395 2564 2849 3007 3124 3265 3292 5120
Size: 1156 169 285 158 117 141 27 1828
Cuts at: 5120 1239
Size: 2579
Fragments arranged by size:
2579 1828 1156 285 169 158 141 117
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4585 5450 5748 4585
Size: 865 298 5297
Fragments arranged by size:
5297 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5123 882
Size: 617 3624 2219
Fragments arranged by size:
3624 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4251 493
Size: 711 3047 2702
Fragments arranged by size:
3047 2702 711
BsaBI GATnn'nnATC
Cuts at: 2657 6454 2657
Size: 3797 2663
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2499 2820 3603
Size: 53 83 186 936 561 405 321 783
Cuts at: 3603 4267 4649 5284 275
Size: 664 382 635 1451
Fragments arranged by size:
1451 936 783 664 635 561 405 382
321 186 83 53
BsaWI w'CCGG_w
Cuts at: 4893 5291 5565 5712 6123 4893
Size: 398 274 147 411 5230
Fragments arranged by size:
5230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2627 2657 3470 3500 1178
Size: 30 165 30 1224 30 813 30 4138
Fragments arranged by size:
4138 1224 813 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 3029 3080 3484 3664 3736 1186
Size: 1558 285 51 404 180 72 3910
Fragments arranged by size:
3910 1558 404 285 180 72 51
BseYI C'CCAG_C
Cuts at: 2951 2991 3404 5663 6159 2951
Size: 40 413 2259 496 3252
Fragments arranged by size:
3252 2259 496 413 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 2507 1163
Size: 573 620 151 5116
Fragments arranged by size:
5116 620 573 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4671 5699 1540
Size: 275 186 1909 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2285 2393 2421 3072 3280 3507 3671 4521 4606
Size: 108 28 651 208 227 164 850 85
Cuts at: 4606 5677 2285
Size: 1071 3068
Fragments arranged by size:
3068 1071 850 651 227 208 164 108
85 28
BsmI GAATG_Cn'
Cuts at: 3971 4064 3971
Size: 93 6367
BsmBI CGTCTCn'nnnn_
Cuts at: 2349 2349
Size: 6460
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2197 2285 2393 2421 2613 2952
Size: 399 354 53 88 108 28 192 339
Cuts at: 2952 3072 3165 3280 3507 3591 3646 3671 3699
Size: 120 93 115 227 84 55 25 28
Cuts at: 3699 3779 4521 4606 5677 1391
Size: 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 339 227 192
120 115 108 93 88 85 84 80
55 53 28 28 25
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 4349 6079 4349
Size: 1730 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2919 3815 844
Size: 2075 896 3489
Fragments arranged by size:
3489 2075 896
BsrBI CCG'CTC
Cuts at: 1394 1823 4347 1394
Size: 429 2524 3507
Fragments arranged by size:
3507 2524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2584 3549 4955 5129 65
Size: 1183 1336 965 1406 174 1396
Fragments arranged by size:
1406 1396 1336 1183 965 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5104 5291 1700
Size: 110 3294 187 2869
Fragments arranged by size:
3294 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 2348 3110 4520 5532 2348
Size: 762 1410 1012 3276
Fragments arranged by size:
3276 1410 1012 762
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6460
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2593 2959 3646 2266
Size: 327 366 687 5080
Fragments arranged by size:
5080 687 366 327
BstYI r'GATC_y
Cuts at: 1972 2118 2439 3039 3790 4157 4542 4559 6011
Size: 146 321 600 751 367 385 17 1452
Cuts at: 6011 6097 6109 6455 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 751 600 385 367 346 321
146 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3063 3748 3063
Size: 685 5775
BtgI C'CryG_G
Cuts at: 513 1065 2613 2976 513
Size: 552 1548 363 3997
Fragments arranged by size:
3997 1548 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5721
Fragments arranged by size:
5721 683 56
BtsI GCAGTG_nn'
Cuts at: 2811 3976 4770 4790 2811
Size: 1165 794 20 4481
Fragments arranged by size:
4481 1165 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6425
DraI TTT'AAA
Cuts at: 3764 3764
Size: 6460
DraIII CAC_nnn'GTG
Cuts at: 2148 3160 2148
Size: 1012 5448
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5467 809
Size: 507 4151 1802
Fragments arranged by size:
4151 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2304 2434
Size: 54 1425 50 275 18 252 222 130
Cuts at: 2434 2515 2808 3907 4796 6425 8
Size: 81 293 1099 889 1629 43
Fragments arranged by size:
1629 1425 1099 889 293 275 252 222
130 81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 1537
Size: 275 2095 4090
Fragments arranged by size:
4090 2095 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2327 3419 3596 3659 4390 1353
Size: 674 300 1092 177 63 731 3423
Fragments arranged by size:
3423 1092 731 674 300 177 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3713 5047 5421 5567 387
Size: 1064 138 2124 1334 374 146 1280
Fragments arranged by size:
2124 1334 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2419 3070 3505 2419
Size: 651 435 5374
Fragments arranged by size:
5374 651 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2395 2564 2849 2990 3007 3124
Size: 393 719 44 169 285 141 17 117
Cuts at: 3124 3265 3292 4523 5120 5907 6175 1239
Size: 141 27 1231 597 787 268 1524
Fragments arranged by size:
1524 1231 787 719 597 393 285 268
169 141 141 117 44 27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2759 2969 3203 3416 2561
Size: 198 210 234 213 5605
Fragments arranged by size:
5605 234 213 210 198
EcoO109I rG'GnC_Cy
Cuts at: 2164 2194 2255 2428 2554 2623 2764 3115 3142
Size: 30 61 173 126 69 141 351 27
Cuts at: 3142 3314 2164
Size: 172 5310
Fragments arranged by size:
5310 351 173 172 141 126 69 61
30 27
EcoRI G'AATT_C
Cuts at: 3149 3769 4147 3149
Size: 620 378 5462
Fragments arranged by size:
5462 620 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 6460
FspI TGC'GCA
Cuts at: 2734 4966 2734
Size: 2232 4228
HaeII r_GCGC'y
Cuts at: 1678 2097 5607 1678
Size: 419 3510 2531
Fragments arranged by size:
3510 2531 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 5101 5133 5175
Size: 32 135 32 728 32 2769 32 42
Cuts at: 5175 5207 1373
Size: 32 2626
Fragments arranged by size:
2769 2626 728 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3804 4050 669
Size: 988 281 1866 246 3079
Fragments arranged by size:
3079 1866 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6460
HpaI GTT'AAC
Cuts at: 4050 4050
Size: 6460
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 6460
KpnI G_GTAC'C
Cuts at: 3785 3785
Size: 6460
MfeI C'AATT_G
Cuts at: 4059 4059
Size: 6460
MluI A'CGCG_T
Cuts at: 6336 6336
Size: 6460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2941 5574 5758 1305
Size: 1636 2633 184 2007
Fragments arranged by size:
2633 2007 1636 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2436 2517 2810 10
Size: 54 1425 343 604 81 293 3660
Fragments arranged by size:
3660 1425 604 343 293 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 2591 4418 4777 4936 518
Size: 648 1311 114 1827 359 159 2042
Fragments arranged by size:
2042 1827 1311 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2455 2719 3876 4553 5701 5946 1606
Size: 849 264 1157 677 1148 245 2120
Fragments arranged by size:
2120 1157 1148 849 677 264 245
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 6460
NcoI C'CATG_G
Cuts at: 513 1065 2613 513
Size: 552 1548 4360
Fragments arranged by size:
4360 1548 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NspI r_CATG'y
Cuts at: 1132 1229 2077 3818 5363 1132
Size: 97 848 1741 1545 2229
Fragments arranged by size:
2229 1741 1545 848 97
PasI CC'CwG_GG
Cuts at: 1348 2197 2320 2503 1348
Size: 849 123 183 5305
Fragments arranged by size:
5305 849 183 123
PciI A'CATG_T
Cuts at: 5359 5359
Size: 6460
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 2401 2692 3175 1108
Size: 1116 177 291 483 4393
Fragments arranged by size:
4393 1116 483 291 177
PfoI T'CCnGG_A
Cuts at: 3269 3417 3269
Size: 148 6312
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3104 3136 4529 4561 6042 6074 3068
Size: 32 4 32 1393 32 1481 32 3454
Fragments arranged by size:
3454 1481 1393 32 32 32 32 4
PpuMI rG'GwC_Cy
Cuts at: 2164 2623 2764 2164
Size: 459 141 5860
Fragments arranged by size:
5860 459 141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 4030 4030
Size: 6460
PspOMI G'GGCC_C
Cuts at: 2193 2193
Size: 6460
PstI C_TGCA'G
Cuts at: 830 2345 2724 3084 3141 3812 830
Size: 1515 379 360 57 671 3478
Fragments arranged by size:
3478 1515 671 379 360 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
PvuII CAG'CTG
Cuts at: 2719 2719
Size: 6460
SacI G_AGCT'C
Cuts at: 2421 3072 3507 2421
Size: 651 435 5374
Fragments arranged by size:
5374 651 435
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SapI GCTCTTCn'nnn_
Cuts at: 2027 2327 3596 3659 2027
Size: 300 1269 63 4828
Fragments arranged by size:
4828 1269 300 63
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 4708 6420 4708
Size: 1712 4748
SfcI C'TryA_G
Cuts at: 826 1045 2125 2341 2720 2843 3080 3137 3361
Size: 219 1080 216 379 123 237 57 224
Cuts at: 3361 3808 4943 5624 5815 826
Size: 447 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 681 447 379 237 224
219 216 191 123 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 6460
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 6460
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4564 5465 5727 820
Size: 197 926 2621 901 262 1553
Fragments arranged by size:
2621 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3818 1132
Size: 2686 3774
SspI AAT'ATT
Cuts at: 5 52 3329 4384 5
Size: 47 3277 1055 2081
Fragments arranged by size:
3277 2081 1055 47
StuI AGG'CCT
Cuts at: 1517 2297 2870 2888 3192 1517
Size: 780 573 18 304 4785
Fragments arranged by size:
4785 780 573 304 18
StyI C'CwwG_G
Cuts at: 513 1065 2053 2431 2613 3899 513
Size: 552 988 378 182 1286 3074
Fragments arranged by size:
3074 1286 988 552 378 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2017 2231 2408 4487 4504 4657 4842
Size: 1255 437 214 177 2079 17 153 185
Cuts at: 4842 5287 325
Size: 445 1498
Fragments arranged by size:
2079 1498 1255 445 437 214 185 177
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3099 4706 6418
Size: 274 80 33 51 1443 1122 1607 1712
Cuts at: 6418 96
Size: 138
Fragments arranged by size:
1712 1607 1443 1122 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 6420 6452 2588
Size: 32 3800 32 2596
Fragments arranged by size:
3800 2596 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2273 1316
Size: 957 5503
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2221 2938 2221
Size: 717 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 4589 4589
Size: 6460
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3604 4268 276
Size: 53 83 186 936 2070 664 2468
Fragments arranged by size:
2468 2070 936 664 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SrfI SwaI