Restriction map for
pFN21AB5059
(Circular) (Six-base) MAPSORT of: pFN21AB5059.seq Check: 9440 from: 1 to: 6571
pFN21AB5059 6571 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3685 3685
Size: 6571
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2319 2354 2493 2612 3109 4634 6018 6286
Size: 687 35 139 119 497 1525 1384 268
Cuts at: 6286 1632
Size: 1917
Fragments arranged by size:
1917 1525 1384 687 497 268 139 119
35
AfeI AGC'GCT
Cuts at: 3654 3654
Size: 6571
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 5470 6447 1203
Size: 4267 977 1327
Fragments arranged by size:
4267 1327 977
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 5300 5300
Size: 6571
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AlwNI CAG_nnn'CTG
Cuts at: 2140 2152 3118 3700 4314 5886 2140
Size: 12 966 582 614 1572 2825
Fragments arranged by size:
2825 1572 966 614 582 12
ApaI G_GGCC'C
Cuts at: 2778 2778
Size: 6571
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3880 4106 4258 6367 1493
Size: 2387 226 152 2109 1697
Fragments arranged by size:
2387 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2965 3278 3580 3797 3896 1870
Size: 73 271 751 313 302 217 99 4545
Fragments arranged by size:
4545 751 313 302 271 217 99 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3064 3097 3064
Size: 33 6538
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2970 3679 3892 5347
Size: 325 217 627 263 920 709 213 1455
Cuts at: 5347 618
Size: 1842
Fragments arranged by size:
1842 1455 920 709 627 325 263 217
213
BanII G_rGCy'C
Cuts at: 1391 2038 2778 3890 1391
Size: 647 740 1112 4072
Fragments arranged by size:
4072 1112 740 647
BbeI G_GCGC'C
Cuts at: 2974 2974
Size: 6571
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2170 3299 928
Size: 971 271 1129 4200
Fragments arranged by size:
4200 1129 971 271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2036 2070 4762 4796 1903
Size: 34 99 34 2692 34 3678
Fragments arranged by size:
3678 2692 99 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2540 4463 5673 2540
Size: 1923 1210 3438
Fragments arranged by size:
3438 1923 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 3431 5182
Size: 107 122 71 631 605 450 1309 1751
Cuts at: 5182 136
Size: 1525
Fragments arranged by size:
1751 1525 1309 631 605 450 122 107
71
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2778 3172 4632 5788 1790
Size: 319 669 394 1460 1156 2573
Fragments arranged by size:
2573 1460 1156 669 394 319
BmgBI CAC'GTC
Cuts at: 1318 3337 3514 1318
Size: 2019 177 4375
Fragments arranged by size:
4375 2019 177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4302 5260 442
Size: 416 53 83 399 24 2885 958 1753
Fragments arranged by size:
2885 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2375 2855 5231 1239
Size: 890 246 480 2376 2579
Fragments arranged by size:
2579 2376 890 480 246
Bpu10I CC'TnA_GC
Cuts at: 2403 2403
Size: 6571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3350 4696 5561 5859 3350
Size: 1346 865 298 4062
Fragments arranged by size:
4062 1346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2921 5234 882
Size: 617 1422 2313 2219
Fragments arranged by size:
2313 2219 1422 617
BsaAI yAC'GTr
Cuts at: 493 1204 2104 2415 4362 493
Size: 711 900 311 1947 2702
Fragments arranged by size:
2702 1947 900 711 311
BsaBI GATnn'nnATC
Cuts at: 2672 6565 2672
Size: 3893 2678
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 3342 4378 4760
Size: 53 83 186 936 1438 371 1036 382
Cuts at: 4760 5395 275
Size: 635 1451
Fragments arranged by size:
1451 1438 1036 936 635 382 371 186
83 53
BsaWI w'CCGG_w
Cuts at: 3558 5004 5402 5676 5823 6234 3558
Size: 1446 398 274 147 411 3895
Fragments arranged by size:
3895 1446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3464 3494 1178
Size: 30 165 30 2061 30 4255
Fragments arranged by size:
4255 2061 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3269 3551 3750 1186
Size: 838 1245 282 199 4007
Fragments arranged by size:
4007 1245 838 282 199
BseYI C'CCAG_C
Cuts at: 3206 5774 6270 3206
Size: 2568 496 3507
Fragments arranged by size:
3507 2568 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5998
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3835 4021 4782 5810 1540
Size: 275 186 1834 186 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 4632 4717 5788 4632
Size: 85 1071 5415
Fragments arranged by size:
5415 1071 85
BsmI GAATG_Cn'
Cuts at: 2604 3434 4082 4175 2604
Size: 830 648 93 5000
Fragments arranged by size:
5000 830 648 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2517 2517
Size: 6571
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2109 2778 3172 3890 4632 4717
Size: 399 248 71 669 394 718 742 85
Cuts at: 4717 5788 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 718 669 399 394 248
85 71
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 4460 6190 4460
Size: 1730 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3282 3366 3685 3808 3926 844
Size: 2438 84 319 123 118 3489
Fragments arranged by size:
3489 2438 319 123 118 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 4458 1394
Size: 429 247 2388 3507
Fragments arranged by size:
3507 2388 429 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 3703 5066 5240 65
Size: 1183 2455 1363 174 1396
Fragments arranged by size:
2455 1396 1363 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 5215 5402 1700
Size: 110 320 3085 187 2869
Fragments arranged by size:
3085 2869 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 2944 3553 96
Size: 2848 609 3114
Fragments arranged by size:
3114 2848 609
BssHII G'CGCG_C
Cuts at: 1925 2303 1925
Size: 378 6193
BssSI C'ACGA_G
Cuts at: 3350 4631 5643 3350
Size: 1281 1012 4278
Fragments arranged by size:
4278 1281 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2140 3679 3700 794
Size: 1346 1539 21 3665
Fragments arranged by size:
3665 1539 1346 21
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 2751 1184
Size: 1567 5004
BstXI CCAn_nnnn'nTGG
Cuts at: 3196 3490 3196
Size: 294 6277
BstYI r'GATC_y
Cuts at: 1972 2349 2628 3901 4268 4653 4670 6122 6208
Size: 377 279 1273 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1273 385 377 367 346 279
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2824 3391 3861 513
Size: 552 939 820 567 470 3223
Fragments arranged by size:
3223 939 820 567 552 470
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2659 3580 487
Size: 683 56 1433 921 3478
Fragments arranged by size:
3478 1433 921 683 56
BtsI GCAGTG_nn'
Cuts at: 4087 4881 4901 4087
Size: 794 20 5757
Fragments arranged by size:
5757 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3457 3492 529
Size: 35 2893 35 3608
Fragments arranged by size:
3608 2893 35 35
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2656 2958 3565
Size: 54 1425 50 275 18 826 302 607
Cuts at: 3565 4018 4907 6536 8
Size: 453 889 1629 43
Fragments arranged by size:
1629 1425 889 826 607 453 302 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4018 1537
Size: 275 2206 4090
Fragments arranged by size:
4090 2206 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 2337 2469 2741 3128 4501 1353
Size: 828 156 132 272 387 1373 3423
Fragments arranged by size:
3423 1373 828 387 272 156 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 5158 5532 5678 387
Size: 1064 138 504 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 504 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2319 2354 2375 2493 2612 2855
Size: 393 497 190 35 21 118 119 243
Cuts at: 2855 3109 4634 5231 6018 6286 1239
Size: 254 1525 597 787 268 1524
Fragments arranged by size:
1525 1524 787 597 497 393 268 254
243 190 119 118 35 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2058 2516 2855 3035 2058
Size: 458 339 180 5594
Fragments arranged by size:
5594 458 339 180
EcoO109I rG'GnC_Cy
Cuts at: 2243 2774 2775 3487 3763 2243
Size: 531 1 712 276 5051
Fragments arranged by size:
5051 712 531 276 1
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 2131 2974 3580 3656 5718 1678
Size: 453 843 606 76 2062 2531
Fragments arranged by size:
2531 2062 843 606 453 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2514 2546 2775 2807 3464
Size: 32 135 32 942 32 229 32 657
Cuts at: 3464 3496 5212 5244 5286 5318 1373
Size: 32 1716 32 42 32 2626
Fragments arranged by size:
2626 1716 942 657 229 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3915 4161 669
Size: 988 281 1977 246 3079
Fragments arranged by size:
3079 1977 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KasI G'GCGC_C
Cuts at: 2970 2970
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1995 2277 2959 5685 5869 1305
Size: 690 282 682 2726 184 2007
Fragments arranged by size:
2726 2007 690 682 282 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 10
Size: 54 1425 343 1128 3621
Fragments arranged by size:
3621 1425 1128 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 2909 2954 3194 4529 4888 5047
Size: 648 1347 396 45 240 1335 359 159
Cuts at: 5047 518
Size: 2042
Fragments arranged by size:
2042 1347 1335 648 396 359 240 159
45
MspA1I CmG'CkG
Cuts at: 1606 2202 2332 2439 3987 4664 5812 6057 1606
Size: 596 130 107 1548 677 1148 245 2120
Fragments arranged by size:
2120 1548 1148 677 596 245 130 107
NaeI GCC'GGC
Cuts at: 2132 2132
Size: 6571
NarI GG'CG_CC
Cuts at: 2971 2971
Size: 6571
NcoI C'CATG_G
Cuts at: 513 1065 2004 2824 3391 3861 513
Size: 552 939 820 567 470 3223
Fragments arranged by size:
3223 939 820 567 552 470
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NgoMIV G'CCGG_C
Cuts at: 2130 2130
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 2470 2908 3532 3929 5474 1132
Size: 97 1241 438 624 397 1545 2229
Fragments arranged by size:
2229 1545 1241 624 438 397 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2512 1108
Size: 1404 5167
PfoI T'CCnGG_A
Cuts at: 2265 2352 2832 3231 3438 2265
Size: 87 480 399 207 5398
Fragments arranged by size:
5398 480 399 207 87
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3015 1613
Size: 1402 5169
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspOMI G'GGCC_C
Cuts at: 2774 2774
Size: 6571
PspXI vC'TCGA_Gb
Cuts at: 3278 3797 3278
Size: 519 6052
PstI C_TGCA'G
Cuts at: 830 2160 2575 2859 2991 3291 3696 3923 830
Size: 1330 415 284 132 300 405 227 3478
Fragments arranged by size:
3478 1330 415 405 300 284 227 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2332 2439 2332
Size: 107 6464
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SapI GCTCTTCn'nnn_
Cuts at: 2181 2469 3128 2181
Size: 288 659 5624
Fragments arranged by size:
5624 659 288
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 2840 4819 6531 2840
Size: 1979 1712 2880
Fragments arranged by size:
2880 1979 1712
SfcI C'TryA_G
Cuts at: 826 1045 2156 2571 2855 2987 3287 3692 3919
Size: 219 1111 415 284 132 300 405 227
Cuts at: 3919 5054 5735 5926 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 415 405 300 284
227 219 191 132
SfoI GGC'GCC
Cuts at: 2972 2972
Size: 6571
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 2216 2967 3898 1872
Size: 344 751 931 4545
Fragments arranged by size:
4545 931 751 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3278 3365 3797 4675 5576 5838
Size: 197 926 1335 87 432 878 901 262
Cuts at: 5838 820
Size: 1553
Fragments arranged by size:
1553 1335 926 901 878 432 262 197
87
SnaBI TAC'GTA
Cuts at: 493 2415 4362 493
Size: 1922 1947 2702
Fragments arranged by size:
2702 1947 1922
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 5499
StyI C'CwwG_G
Cuts at: 513 1065 2004 2319 2824 3391 3483 3861 4010
Size: 552 939 315 505 567 92 378 149
Cuts at: 4010 513
Size: 3074
Fragments arranged by size:
3074 939 567 552 505 378 315 149
92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 2628 2635 3821 4598 4615 4768
Size: 1255 974 74 7 1186 777 17 153
Cuts at: 4768 4953 5398 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1186 974 777 445 185 153
74 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2838 2944 3307
Size: 274 80 33 51 1443 861 106 363
Cuts at: 3307 3553 4817 6529 96
Size: 246 1264 1712 138
Fragments arranged by size:
1712 1443 1264 861 363 274 246 138
106 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6531 6563 6531
Size: 32 6539
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 3278 3797 1943
Size: 1335 519 4717
Fragments arranged by size:
4717 1335 519
XmaI C'CCGG_G
Cuts at: 1870 2214 2965 3896 1870
Size: 344 751 931 4545
Fragments arranged by size:
4545 931 751 344
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI