Restriction map for
pFN21AB5056
(Circular) (Six-base) MAPSORT of: pFN21AB5056.seq Check: 8517 from: 1 to: 6376
pFN21AB5056 6376 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2571 4186 278
Size: 53 83 186 936 1035 1615 2468
Fragments arranged by size:
2468 1615 1035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 3719 1937
Size: 634 1148 4594
Fragments arranged by size:
4594 1148 634
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2221 2297 2509 3052 4439 5823 6091 1632
Size: 589 76 212 543 1387 1384 268 1917
Fragments arranged by size:
1917 1387 1384 589 543 268 212 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 3557 3567 5275 6252 1203
Size: 2354 10 1708 977 1327
Fragments arranged by size:
2354 1708 1327 977 10
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 2392 3489 5105 2392
Size: 1097 1616 3663
Fragments arranged by size:
3663 1616 1097
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 2220 4119 5691 2220
Size: 1899 1572 2905
Fragments arranged by size:
2905 1899 1572
ApaI G_GGCC'C
Cuts at: 2054 2314 2560 3129 2054
Size: 260 246 569 5301
Fragments arranged by size:
5301 569 260 246
ApaLI G'TGCA_C
Cuts at: 3514 4433 5589 3514
Size: 919 1156 4301
Fragments arranged by size:
4301 1156 919
ApoI r'AATT_y
Cuts at: 1493 3098 3685 3911 4063 6172 1493
Size: 1605 587 226 152 2109 1697
Fragments arranged by size:
2109 1697 1605 587 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2986 3122 3470 3701 1870
Size: 73 1043 136 348 231 4545
Fragments arranged by size:
4545 1043 348 231 136 73
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3697 5152 618
Size: 325 217 627 1910 1455 1842
Fragments arranged by size:
1910 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2127 2168 2314 2560 3129 3695 1391
Size: 663 73 41 146 246 569 566 4072
Fragments arranged by size:
4072 663 569 566 246 146 73 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2479 2923 928
Size: 971 580 444 4381
Fragments arranged by size:
4381 971 580 444
BbvCI CC'TCA_GC
Cuts at: 2186 3185 2186
Size: 999 5377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2437 2471 4567 4601 1903
Size: 34 500 34 2096 34 3678
Fragments arranged by size:
3678 2096 500 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4268 5478 3329
Size: 939 1210 4227
Fragments arranged by size:
4227 1210 939
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2753 3195 4987
Size: 107 122 71 631 605 1081 442 1792
Cuts at: 4987 136
Size: 1525
Fragments arranged by size:
1792 1525 1081 631 605 442 122 107
71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 3398 3793 3398
Size: 395 5981
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2314 2560 3123 3129 3370 3518 4437
Size: 264 260 246 563 6 241 148 919
Cuts at: 4437 5593 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 919 563 264 260 246 241
148 6
BmgBI CAC'GTC
Cuts at: 1318 2866 1318
Size: 1548 4828
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4107 5065
Size: 416 53 83 399 24 1940 750 958
Cuts at: 5065 442
Size: 1753
Fragments arranged by size:
1940 1753 958 750 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2323 5036 1239
Size: 1084 2713 2579
Fragments arranged by size:
2713 2579 1084
Bpu10I CC'TnA_GC
Cuts at: 2186 3185 2186
Size: 999 5377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3584 4501 5366 5664 3584
Size: 917 865 298 4296
Fragments arranged by size:
4296 917 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 5039 882
Size: 617 1455 2085 2219
Fragments arranged by size:
2219 2085 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 3030 3405 4167 493
Size: 711 1826 375 762 2702
Fragments arranged by size:
2702 1826 762 711 375
BsaBI GATnn'nnATC
Cuts at: 3284 6370 3284
Size: 3086 3290
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2568 2589 3021 3228
Size: 53 83 186 936 1035 21 432 207
Cuts at: 3228 4183 4565 5200 275
Size: 955 382 635 1451
Fragments arranged by size:
1451 1035 955 936 635 432 382 207
186 83 53 21
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3656 3686 1178
Size: 30 165 30 2253 30 3868
Fragments arranged by size:
3868 2253 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 1186
Size: 1258 5118
BseYI C'CCAG_C
Cuts at: 2114 2128 2320 5579 6075 2114
Size: 14 192 3259 496 2415
Fragments arranged by size:
3259 2415 496 192 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3116 3164 3245 3427 3596 1163
Size: 573 1380 48 81 182 169 3943
Fragments arranged by size:
3943 1380 573 182 169 81 48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2544 2636 2663 2757 3053 3057
Size: 275 186 543 92 27 94 296 4
Cuts at: 3057 3204 3826 4587 5615 1540
Size: 147 622 761 1028 2301
Fragments arranged by size:
2301 1028 761 622 543 296 275 186
147 94 92 27 4
BsiHKAI G_wGCw'C
Cuts at: 2030 2619 2907 3518 4437 4522 5593 2030
Size: 589 288 611 919 85 1071 2813
Fragments arranged by size:
2813 1071 919 611 589 288 85
BsmI GAATG_Cn'
Cuts at: 2459 3887 3980 2459
Size: 1428 93 4855
Fragments arranged by size:
4855 1428 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2054 2127 2168 2314 2560 2619
Size: 399 240 24 73 41 146 246 59
Cuts at: 2619 2907 3123 3129 3370 3518 3695 4437 4522
Size: 288 216 6 241 148 177 742 85
Cuts at: 4522 5593 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 288 246 241 240
216 177 148 146 85 73 59 41
24 6
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2841 3069 3731 844
Size: 1997 228 662 3489
Fragments arranged by size:
3489 1997 662 228
BsrBI CCG'CTC
Cuts at: 1394 1823 2307 2551 3313 4263 1394
Size: 429 484 244 762 950 3507
Fragments arranged by size:
3507 950 762 484 429 244
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2752 2759 2812 2855 2940 3052 3206
Size: 110 942 7 53 43 85 112 154
Cuts at: 3206 5020 5207 1700
Size: 1814 187 2869
Fragments arranged by size:
2869 1814 942 187 154 112 110 85
53 43 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 2690 2805 2851 2859 2894 2920 2922 2994
Size: 765 115 46 8 35 26 2 72
Cuts at: 2994 3006 1925
Size: 12 5295
Fragments arranged by size:
5295 765 115 72 46 35 26 12
8 2
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3522 794
Size: 2728 3648
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 3304 3370 3304
Size: 66 6310
BstYI r'GATC_y
Cuts at: 1972 2172 3279 3706 4073 4458 4475 5927 6013
Size: 200 1107 427 367 385 17 1452 86
Cuts at: 6013 6025 6371 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1107 427 385 367 346 200
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2427 3075 2427
Size: 648 5728
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4885
Fragments arranged by size:
4885 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2440 3522 487
Size: 683 56 1214 1082 3341
Fragments arranged by size:
3341 1214 1082 683 56
BtsI GCAGTG_nn'
Cuts at: 2112 2300 3892 4686 4706 2112
Size: 188 1592 794 20 3782
Fragments arranged by size:
3782 1592 794 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DraIII CAC_nnn'GTG
Cuts at: 3030 3538 3030
Size: 508 5868
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2541 2633 2660
Size: 54 1425 50 275 18 711 92 27
Cuts at: 2660 2754 2955 3050 3054 3201 3306 3562 3823
Size: 94 201 95 4 147 105 256 261
Cuts at: 3823 4712 6341 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 711 275 261 256 201
147 105 95 94 92 54 50 43
27 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2541 2633 2660 2754 3050 3054 3201
Size: 275 729 92 27 94 296 4 147
Cuts at: 3201 3823 1537
Size: 622 4090
Fragments arranged by size:
4090 729 622 296 275 147 94 92
27 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 3257 4306 1353
Size: 797 1107 1049 3423
Fragments arranged by size:
3423 1107 1049 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 4963 5337 5483 387
Size: 1064 138 1107 2267 374 146 1280
Fragments arranged by size:
2267 1280 1107 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2297 2323 2509 3052 4439 5036
Size: 393 589 76 26 186 543 1387 597
Cuts at: 5036 5823 6091 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1387 787 597 589 543 393 268
186 76 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3602 3602
Size: 6376
EcoO109I rG'GnC_Cy
Cuts at: 2050 2051 2310 2311 2338 2557 3079 3126 2050
Size: 1 259 1 27 219 522 47 5300
Fragments arranged by size:
5300 522 259 219 47 27 1 1
EcoRI G'AATT_C
Cuts at: 3098 3685 4063 3098
Size: 587 378 5411
Fragments arranged by size:
5411 587 378
FseI GG_CCGG'CC
Cuts at: 3056 3056
Size: 6376
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2423 5523 1678
Size: 745 3100 2531
Fragments arranged by size:
3100 2531 745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 3475 3507 5017
Size: 32 135 32 806 32 1065 32 1510
Cuts at: 5017 5049 5091 5123 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1510 1065 806 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3163 3720 3966 669
Size: 988 281 1225 557 246 3079
Fragments arranged by size:
3079 1225 988 557 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5490 5674 1305
Size: 4185 184 2007
Fragments arranged by size:
4185 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3564 10
Size: 54 1425 343 1732 2822
Fragments arranged by size:
2822 1732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4334 4693 4852 518
Size: 648 3168 359 159 2042
Fragments arranged by size:
3168 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2140 2529 2596 3138 3217 3792 4469
Size: 512 22 389 67 542 79 575 677
Cuts at: 4469 5617 5862 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 575 542 512 389 245
79 67 22
NaeI GCC'GGC
Cuts at: 2754 2761 2814 2857 2942 3054 3208 2754
Size: 7 53 43 85 112 154 5922
Fragments arranged by size:
5922 154 112 85 53 43 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4885
Fragments arranged by size:
4885 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NgoMIV G'CCGG_C
Cuts at: 2752 2759 2812 2855 2940 3052 3206 2752
Size: 7 53 43 85 112 154 5922
Fragments arranged by size:
5922 154 112 85 53 43 7
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 2633 3201 3823 2633
Size: 568 622 5186
Fragments arranged by size:
5186 622 568
NspI r_CATG'y
Cuts at: 1132 1229 3214 3561 3571 3734 5279 1132
Size: 97 1985 347 10 163 1545 2229
Fragments arranged by size:
2229 1985 1545 347 163 97 10
PasI CC'CwG_GG
Cuts at: 1348 3370 1348
Size: 2022 4354
PciI A'CATG_T
Cuts at: 3557 3567 5275 3557
Size: 10 1708 4658
Fragments arranged by size:
4658 1708 10
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6376
PfoI T'CCnGG_A
Cuts at: 2619 2619
Size: 6376
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PmlI CAC'GTG
Cuts at: 3030 3405 3030
Size: 375 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2338 2338
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 2050 2310 2556 3125 2050
Size: 260 246 569 5301
Fragments arranged by size:
5301 569 260 246
PstI C_TGCA'G
Cuts at: 830 3728 830
Size: 2898 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2596 3217 2596
Size: 621 5755
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SapI GCTCTTCn'nnn_
Cuts at: 2150 3257 2150
Size: 1107 5269
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SexAI A'CCwGG_T
Cuts at: 2388 2388
Size: 6376
SfcI C'TryA_G
Cuts at: 826 1045 3724 4859 5540 5731 826
Size: 219 2679 1135 681 191 1471
Fragments arranged by size:
2679 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2753 2753
Size: 6376
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 2855 1700
Size: 1155 5221
SmaI CCC'GGG
Cuts at: 1872 3124 3703 1872
Size: 1252 579 4545
Fragments arranged by size:
4545 1252 579
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3599 4480 5381 5643 820
Size: 197 926 1656 881 901 262 1553
Fragments arranged by size:
1656 1553 926 901 881 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3214 3734 1132
Size: 2082 520 3774
Fragments arranged by size:
3774 2082 520
SrfI GCCC'GGGC
Cuts at: 3124 3124
Size: 6376
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2415 3815 513
Size: 552 939 156 255 1400 3074
Fragments arranged by size:
3074 1400 939 552 255 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2455 4403 4420 4573 4758 5203 325
Size: 1255 875 1948 17 153 185 445 1498
Fragments arranged by size:
1948 1498 1255 875 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4622 6334 96
Size: 274 80 33 51 1443 2645 1712 138
Fragments arranged by size:
2645 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6336 6368 6336
Size: 32 6344
Tth111I GACn'n_nGTC
Cuts at: 1316 3545 1316
Size: 2229 4147
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3122 3701 1870
Size: 1252 579 4545
Fragments arranged by size:
4545 1252 579
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2569 4184 276
Size: 53 83 186 936 1035 1615 2468
Fragments arranged by size:
2468 1615 1035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SanDI SfoI SwaI XcmI