Restriction map for
pFN21AB5055
(Circular) (Six-base) MAPSORT of: pFN21AB5055.seq Check: 1944 from: 1 to: 6247
pFN21AB5055 6247 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2942 2942
Size: 6247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3590 1937
Size: 1653 4594
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 2705 4310 5694 5962 1632
Size: 974 99 1605 1384 268 1917
Fragments arranged by size:
1917 1605 1384 974 268 99
AflII C'TTAA_G
Cuts at: 820 1017 2186 3467 820
Size: 197 1169 1281 3600
Fragments arranged by size:
3600 1281 1169 197
AflIII A'CryG_T
Cuts at: 1203 5146 6123 1203
Size: 3943 977 1327
Fragments arranged by size:
3943 1327 977
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 3355 4976 3355
Size: 1621 4626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 2231 2357 2737 3990 5562 2231
Size: 126 380 1253 1572 2916
Fragments arranged by size:
2916 1572 1253 380 126
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2297 2601 2839 3368 3556 3782 3934 6043
Size: 804 304 238 529 188 226 152 2109
Cuts at: 6043 1493
Size: 1697
Fragments arranged by size:
2109 1697 804 529 304 238 226 188
152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3219 3577 3944 3219
Size: 358 367 5522
Fragments arranged by size:
5522 367 358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3419 3568 5023 618
Size: 325 217 627 1632 149 1455 1842
Fragments arranged by size:
1842 1632 1455 627 325 217 149
BanII G_rGCy'C
Cuts at: 1391 3167 3566 1391
Size: 1776 399 4072
Fragments arranged by size:
4072 1776 399
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 928
Size: 971 660 4616
Fragments arranged by size:
4616 971 660
BbvCI CC'TCA_GC
Cuts at: 2366 3275 3490 2366
Size: 909 215 5123
Fragments arranged by size:
5123 909 215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 4438 4472 1903
Size: 34 183 34 2284 34 3678
Fragments arranged by size:
3678 2284 183 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2438 4139 5349 2438
Size: 1701 1210 3336
Fragments arranged by size:
3336 1701 1210
BclI T'GATC_A
Cuts at: 1548 2862 1548
Size: 1314 4933
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3143 4858 136
Size: 107 122 71 631 605 1471 1715 1525
Fragments arranged by size:
1715 1525 1471 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 3664 3664
Size: 6247
Bme1580I G_kGCm'C
Cuts at: 1790 2033 4308 5464 1790
Size: 243 2275 1156 2573
Fragments arranged by size:
2573 2275 1156 243
BmgBI CAC'GTC
Cuts at: 1318 2034 1318
Size: 716 5531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2467 4907 1239
Size: 1228 2440 2579
Fragments arranged by size:
2579 2440 1228
Bpu10I CC'TnA_GC
Cuts at: 2366 3275 3490 2366
Size: 909 215 5123
Fragments arranged by size:
5123 909 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 4038 493
Size: 711 837 1997 2702
Fragments arranged by size:
2702 1997 837 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3510 4054 4436 5071
Size: 53 83 186 936 1977 544 382 635
Cuts at: 5071 275
Size: 1451
Fragments arranged by size:
1977 1451 936 635 544 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3082 4680 5078 5352 5499 5910 3082
Size: 1598 398 274 147 411 3419
Fragments arranged by size:
3419 1598 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2898 2928 3455 3485 1178
Size: 30 165 30 1495 30 527 30 3940
Fragments arranged by size:
3940 1495 527 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2470 2522 1186
Size: 1284 52 4911
Fragments arranged by size:
4911 1284 52
BseYI C'CCAG_C
Cuts at: 2231 5450 5946 2231
Size: 3219 496 2532
Fragments arranged by size:
3219 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3365 1163
Size: 573 1629 4045
Fragments arranged by size:
4045 1629 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4308 4393 5464 4308
Size: 85 1071 5091
Fragments arranged by size:
5091 1071 85
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
BsmBI CGTCTCn'nnnn_
Cuts at: 2079 2079
Size: 6247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 3167 3566 4308 4393 5464 1391
Size: 399 243 1134 399 742 85 1071 2174
Fragments arranged by size:
2174 1134 1071 742 399 399 243 85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 2761 3156 4136 5866 2761
Size: 395 980 1730 3142
Fragments arranged by size:
3142 1730 980 395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2942 3602 844
Size: 2098 660 3489
Fragments arranged by size:
3489 2098 660
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2253 2667 2917 3087 4742 4916 65
Size: 1183 1005 414 250 170 1655 174 1396
Fragments arranged by size:
1655 1396 1183 1005 414 250 174 170
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2288 3045 96
Size: 2192 757 3298
Fragments arranged by size:
3298 2192 757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3010 794
Size: 2216 4031
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2903 3416 2248
Size: 655 513 5079
Fragments arranged by size:
5079 655 513
BstYI r'GATC_y
Cuts at: 1972 3219 3577 3944 4329 4346 5798 5884 5896
Size: 1247 358 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1247 385 367 358 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 3261 513
Size: 552 939 207 1050 3499
Fragments arranged by size:
3499 1050 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 2136 2919 3048 3763 4557 4577 2136
Size: 783 129 715 794 20 3806
Fragments arranged by size:
3806 794 783 715 129 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2360 5254 809
Size: 507 1044 2894 1802
Fragments arranged by size:
2894 1802 1044 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2238 2661
Size: 54 1425 50 275 18 177 231 423
Cuts at: 2661 3694 4583 6212 8
Size: 1033 889 1629 43
Fragments arranged by size:
1629 1425 1033 889 423 275 231 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2526 2833 3473 4177 1353
Size: 725 448 307 640 704 3423
Fragments arranged by size:
3423 725 704 640 448 307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2467 2606 2705 4310 4907 5694 5962
Size: 393 835 139 99 1605 597 787 268
Cuts at: 5962 1239
Size: 1524
Fragments arranged by size:
1605 1524 835 787 597 393 268 139
99
EcoO109I rG'GnC_Cy
Cuts at: 3144 3190 3144
Size: 46 6201
EcoRI G'AATT_C
Cuts at: 2297 3556 3934 2297
Size: 1259 378 4610
Fragments arranged by size:
4610 1259 378
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 2255 3073 5394 1678
Size: 577 818 2321 2531
Fragments arranged by size:
2531 2321 818 577
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 3151 3183 3341
Size: 32 135 32 577 32 970 32 158
Cuts at: 3341 3373 4888 4920 4962 4994 1373
Size: 32 1515 32 42 32 2626
Fragments arranged by size:
2626 1515 970 577 158 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6247
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3421 5361 5545 1305
Size: 2116 1940 184 2007
Fragments arranged by size:
2116 2007 1940 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 2663 10
Size: 54 1425 343 408 423 3594
Fragments arranged by size:
3594 1425 423 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 2766 3155 3528 4205 4564 4723
Size: 648 1080 520 389 373 677 359 159
Cuts at: 4723 518
Size: 2042
Fragments arranged by size:
2042 1080 677 648 520 389 373 359
159
MspA1I CmG'CkG
Cuts at: 1606 2734 3663 4340 5488 5733 1606
Size: 1128 929 677 1148 245 2120
Fragments arranged by size:
2120 1148 1128 929 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2211 3261 513
Size: 552 939 207 1050 3499
Fragments arranged by size:
3499 1050 939 552 207
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3605 5150 1132
Size: 97 2376 1545 2229
Fragments arranged by size:
2376 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2120 2833 3325 1348
Size: 772 713 492 4270
Fragments arranged by size:
4270 772 713 492
PciI A'CATG_T
Cuts at: 5146 5146
Size: 6247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3112 1108
Size: 2004 4243
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3437 1613
Size: 1824 4423
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PstI C_TGCA'G
Cuts at: 830 2143 2575 2610 3599 830
Size: 1313 432 35 989 3478
Fragments arranged by size:
3478 1313 989 432 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2734 2734
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SapI GCTCTTCn'nnn_
Cuts at: 2078 2078
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 2575 3599 2575
Size: 1024 5223
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SexAI A'CCwGG_T
Cuts at: 2145 2145
Size: 6247
SfcI C'TryA_G
Cuts at: 826 1045 2139 2571 2606 3595 4730 5411 5602
Size: 219 1094 432 35 989 1135 681 191
Cuts at: 5602 826
Size: 1471
Fragments arranged by size:
1471 1135 1094 989 681 432 219 191
35
SfiI GGCCn_nnn'nGGCC
Cuts at: 3143 3143
Size: 6247
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 3467 4351 5252 5514 820
Size: 197 926 243 1281 884 901 262 1553
Fragments arranged by size:
1553 1281 926 901 884 262 243 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 4171 5
Size: 47 4119 2081
Fragments arranged by size:
4119 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2373 2403 3132 3139 3261
Size: 552 939 207 162 30 729 7 122
Cuts at: 3261 3686 513
Size: 425 3074
Fragments arranged by size:
3074 939 729 552 425 207 162 122
30 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4274 4291 4444 4629 5074 325
Size: 1255 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2288 2868 3045
Size: 274 80 33 51 1443 311 580 177
Cuts at: 3045 3453 4493 6205 96
Size: 408 1040 1712 138
Fragments arranged by size:
1712 1443 1040 580 408 311 274 177
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2283 2315 2898 2930 6207 6239 2283
Size: 32 583 32 3277 32 2291
Fragments arranged by size:
3277 2291 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2219 1316
Size: 903 5344
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3157 3332 3503 3157
Size: 175 171 5901
Fragments arranged by size:
5901 175 171
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 4376 4376
Size: 6247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfoI SrfI SwaI