Restriction map for
pFN21AB5053
(Circular) (Six-base) MAPSORT of: pFN21AB5053.seq Check: 5360 from: 1 to: 7045
pFN21AB5053 7045 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2465 2465
Size: 7045
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4855 278
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 4388 1937
Size: 883 1568 4594
Fragments arranged by size:
4594 1568 883
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 5172 5545 1563
Size: 3609 373 3063
Fragments arranged by size:
3609 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2696 2771 3059 4121 4211 4238 5108 6492
Size: 1064 75 288 1062 90 27 870 1384
Cuts at: 6492 6760 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1064 1062 870 288 268 90
75 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 5944 6921 1203
Size: 4741 977 1327
Fragments arranged by size:
4741 1327 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 2888 1166
Size: 1722 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7045
AlwNI CAG_nnn'CTG
Cuts at: 3197 3214 3697 3706 3803 4114 4189 4788 6360
Size: 17 483 9 97 311 75 599 1572
Cuts at: 6360 3197
Size: 3882
Fragments arranged by size:
3882 1572 599 483 311 97 75 17
9
ApaI G_GGCC'C
Cuts at: 3060 3060
Size: 7045
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 2966 4354 4580 4732 6841 1493
Size: 1473 1388 226 152 2109 1697
Fragments arranged by size:
2109 1697 1473 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5599 160
Size: 872 4567 1606
Fragments arranged by size:
4567 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 4009 4370 1870
Size: 73 1512 554 361 4545
Fragments arranged by size:
4545 1512 554 361 73
BamHI G'GATC_C
Cuts at: 2661 4083 4375 4742 2661
Size: 1422 292 367 4964
Fragments arranged by size:
4964 1422 367 292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2477 2633 2983 3863
Size: 325 217 627 221 469 156 350 880
Cuts at: 3863 4366 5821 618
Size: 503 1455 1842
Fragments arranged by size:
1842 1455 880 627 503 469 350 325
221 217 156
BanII G_rGCy'C
Cuts at: 1391 2121 2714 3060 3683 4010 4364 1391
Size: 730 593 346 623 327 354 4072
Fragments arranged by size:
4072 730 623 593 354 346 327
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 7045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2949 928
Size: 971 1050 5024
Fragments arranged by size:
5024 1050 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3446 3480 5236 5270 1903
Size: 34 1509 34 1756 34 3678
Fragments arranged by size:
3678 1756 1509 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4937 6147 4937
Size: 1210 5835
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7045
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2318 2610
Size: 107 122 71 631 605 427 219 292
Cuts at: 2610 3209 5656 136
Size: 599 2447 1525
Fragments arranged by size:
2447 1525 631 605 599 427 292 219
122 107 71
BglII A'GATC_T
Cuts at: 3306 7040 3306
Size: 3734 3311
BlpI GC'TnA_GC
Cuts at: 3190 4462 3190
Size: 1272 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2684 3060 3554 3831 4318 5106 6262 1790
Size: 894 376 494 277 487 788 1156 2573
Fragments arranged by size:
2573 1156 894 788 494 487 376 277
BmgBI CAC'GTC
Cuts at: 1318 3477 3676 1318
Size: 2159 199 4687
Fragments arranged by size:
4687 2159 199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3235 4776 5734
Size: 416 53 83 399 24 1818 1541 958
Cuts at: 5734 442
Size: 1753
Fragments arranged by size:
1818 1753 1541 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 3240 3596 3728 3761 4052 5705 1239
Size: 1073 928 356 132 33 291 1653 2579
Fragments arranged by size:
2579 1653 1073 928 356 291 132 33
Bpu10I CC'TnA_GC
Cuts at: 2924 2924
Size: 7045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3212 3331 3503 3800 5170 6035 6333 3212
Size: 119 172 297 1370 865 298 3924
Fragments arranged by size:
3924 1370 865 298 297 172 119
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3452 3527 4085 4247 5708 882
Size: 617 1953 75 558 162 1461 2219
Fragments arranged by size:
2219 1953 1461 617 558 162 75
BsaAI yAC'GTr
Cuts at: 493 1204 4836 493
Size: 711 3632 2702
Fragments arranged by size:
3632 2702 711
BsaBI GATnn'nnATC
Cuts at: 3758 7039 3758
Size: 3281 3764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4852 5234 5869
Size: 53 83 186 936 476 2843 382 635
Cuts at: 5869 275
Size: 1451
Fragments arranged by size:
2843 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2720 3583 5478 5876 6150 6297 6708 2720
Size: 863 1895 398 274 147 411 3057
Fragments arranged by size:
3057 1895 863 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2663 2693 2813 2843 3238
Size: 30 165 30 1260 30 120 30 395
Cuts at: 3238 3268 1178
Size: 30 4955
Fragments arranged by size:
4955 1260 395 165 120 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7045
BseYI C'CCAG_C
Cuts at: 2657 6248 6744 2657
Size: 3591 496 2958
Fragments arranged by size:
3591 2958 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2818 2886 3142 1163
Size: 573 1082 68 256 5066
Fragments arranged by size:
5066 1082 573 256 68
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2189 2282 2938 4495 5256 6284
Size: 275 186 188 93 656 1557 761 1028
Cuts at: 6284 1540
Size: 2301
Fragments arranged by size:
2301 1557 1028 761 656 275 188 186
93
BsiHKAI G_wGCw'C
Cuts at: 5106 5191 6262 5106
Size: 85 1071 5889
Fragments arranged by size:
5889 1071 85
BsmI GAATG_Cn'
Cuts at: 4556 4649 4556
Size: 93 6952
BsmBI CGTCTCn'nnnn_
Cuts at: 3981 4277 3981
Size: 296 6749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2684 2714 3060 3554 3683 3831
Size: 399 331 563 30 346 494 129 148
Cuts at: 3831 4010 4318 4364 5106 5191 6262 1391
Size: 179 308 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 494 399 346 331
308 179 148 129 85 46 30
BspEI T'CCGG_A
Cuts at: 3583 6708 3583
Size: 3125 3920
BspHI T'CATG_A
Cuts at: 4934 6664 4934
Size: 1730 5315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 2277 2430 2465 3756 3861 4400 844
Size: 1307 126 153 35 1291 105 539 3489
Fragments arranged by size:
3489 1307 1291 539 153 126 105 35
BsrBI CCG'CTC
Cuts at: 1394 1823 3289 4932 1394
Size: 429 1466 1643 3507
Fragments arranged by size:
3507 1643 1466 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2946 3504 3910 5540 5714 65
Size: 1183 1698 558 406 1630 174 1396
Fragments arranged by size:
1698 1630 1396 1183 558 406 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 2933 5689 5876 1700
Size: 110 374 749 2756 187 2869
Fragments arranged by size:
2869 2756 749 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 4088 5105 6117 4088
Size: 1017 1012 5016
Fragments arranged by size:
5016 1017 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7045
BstBI TT'CG_AA
Cuts at: 5849 5849
Size: 7045
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7045
BstXI CCAn_nnnn'nTGG
Cuts at: 2813 2813
Size: 7045
BstYI r'GATC_y
Cuts at: 1972 2510 2661 3270 3306 3316 3753 4083 4375
Size: 538 151 609 36 10 437 330 292
Cuts at: 4375 4742 5127 5144 6596 6682 6694 7040 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 609 538 437 385 367 346
330 292 151 86 36 17 12 10
BtgI C'CryG_G
Cuts at: 513 1065 2004 2806 3220 3823 513
Size: 552 939 802 414 603 3735
Fragments arranged by size:
3735 939 802 603 552 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2117 487
Size: 683 56 891 5415
Fragments arranged by size:
5415 891 683 56
BtsI GCAGTG_nn'
Cuts at: 3939 4318 4561 5355 5375 3939
Size: 379 243 794 20 5609
Fragments arranged by size:
5609 794 379 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3328 3363 3805 3840 529
Size: 35 2764 35 442 35 3734
Fragments arranged by size:
3734 2764 442 35 35 35
DraI TTT'AAA
Cuts at: 4349 4349
Size: 7045
DraIII CAC_nnn'GTG
Cuts at: 2983 3085 4165 2983
Size: 102 1080 5863
Fragments arranged by size:
5863 1080 102
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6052 809
Size: 507 4736 1802
Fragments arranged by size:
4736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2186 2279 2292
Size: 54 1425 50 275 18 356 93 13
Cuts at: 2292 2319 2935 3201 3336 3361 3501 4492 5381
Size: 27 616 266 135 25 140 991 889
Cuts at: 5381 7010 8
Size: 1629 43
Fragments arranged by size:
1629 1425 991 889 616 356 275 266
140 135 93 54 50 43 27 25
18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2186 2279 2935 4492 1537
Size: 275 374 93 656 1557 4090
Fragments arranged by size:
4090 1557 656 374 275 93
EarI CTCTTCn'nnn_
Cuts at: 1353 4975 1353
Size: 3622 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5632 6006 6152 387
Size: 1064 138 4043 374 146 1280
Fragments arranged by size:
4043 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2696 2771 3059 3240 3596 3728
Size: 393 680 384 75 288 181 356 132
Cuts at: 3728 3761 4052 4121 4211 4238 5108 5705 6492
Size: 33 291 69 90 27 870 597 787
Cuts at: 6492 6760 1239
Size: 268 1524
Fragments arranged by size:
1524 870 787 680 597 393 384 356
291 288 268 181 132 90 75 69
33 27
EcoNI CCTnn'n_nnAGG
Cuts at: 3421 3761 3421
Size: 340 6705
EcoO109I rG'GnC_Cy
Cuts at: 3057 3736 3745 4111 4186 4245 3057
Size: 679 9 366 75 59 5857
Fragments arranged by size:
5857 679 366 75 59 9
EcoRI G'AATT_C
Cuts at: 2966 4354 4732 2966
Size: 1388 378 5279
Fragments arranged by size:
5279 1388 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 7045
FspI TGC'GCA
Cuts at: 3902 5551 3902
Size: 1649 5396
HaeII r_GCGC'y
Cuts at: 1678 2012 3190 3697 6192 1678
Size: 334 1178 507 2495 2531
Fragments arranged by size:
2531 2495 1178 507 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2534 2698 2730 2813
Size: 32 135 32 930 32 164 32 83
Cuts at: 2813 2845 3779 3811 3827 3859 5686 5718 5760
Size: 32 934 32 16 32 1827 32 42
Cuts at: 5760 5792 1373
Size: 32 2626
Fragments arranged by size:
2626 1827 934 930 164 135 83 42
32 32 32 32 32 32 32 32
32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 4389 4635 669
Size: 988 281 2451 246 3079
Fragments arranged by size:
3079 2451 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7045
HpaI GTT'AAC
Cuts at: 4635 4635
Size: 7045
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 7045
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 4644 4644
Size: 7045
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3870 6159 6343 1305
Size: 2565 2289 184 2007
Fragments arranged by size:
2565 2289 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2321 3203 3338 3363
Size: 54 1425 343 462 27 882 135 25
Cuts at: 3363 3503 10
Size: 140 3552
Fragments arranged by size:
3552 1425 882 462 343 140 135 54
27 25
MslI CAynn'nnrTG
Cuts at: 518 1166 2226 2840 2888 3051 3137 5003 5362
Size: 648 1060 614 48 163 86 1866 359
Cuts at: 5362 5521 518
Size: 159 2042
Fragments arranged by size:
2042 1866 1060 648 614 359 163 159
86 48
MspA1I CmG'CkG
Cuts at: 1606 2191 2343 3197 3660 3800 4461 5138 6286
Size: 585 152 854 463 140 661 677 1148
Cuts at: 6286 6531 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 854 677 661 585 463 245
152 140
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 7045
NcoI C'CATG_G
Cuts at: 513 1065 2004 3220 513
Size: 552 939 1216 4338
Fragments arranged by size:
4338 1216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7045
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 3671 4025 4403 5948 1132
Size: 97 2442 354 378 1545 2229
Fragments arranged by size:
2442 2229 1545 378 354 97
PasI CC'CwG_GG
Cuts at: 1348 2667 3620 1348
Size: 1319 953 4773
Fragments arranged by size:
4773 1319 953
PciI A'CATG_T
Cuts at: 5944 5944
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7045
PfoI T'CCnGG_A
Cuts at: 3705 3705
Size: 7045
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 6627 6659 5114
Size: 32 1481 32 5500
Fragments arranged by size:
5500 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3736 3745 4245 3736
Size: 9 500 6536
Fragments arranged by size:
6536 500 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7045
PsiI TTA'TAA
Cuts at: 2457 4615 2457
Size: 2158 4887
PspOMI G'GGCC_C
Cuts at: 3056 3056
Size: 7045
PspXI vC'TCGA_Gb
Cuts at: 4009 4009
Size: 7045
PstI C_TGCA'G
Cuts at: 830 2040 2476 3702 3753 4397 830
Size: 1210 436 1226 51 644 3478
Fragments arranged by size:
3478 1226 1210 644 436 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 3197 3800 3197
Size: 603 6442
RsrII CG'GwC_CG
Cuts at: 2284 2284
Size: 7045
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SanDI GG'GwC_CC
Cuts at: 4245 4245
Size: 7045
SbfI CC_TGCA'GG
Cuts at: 4397 4397
Size: 7045
ScaI AGT'ACT
Cuts at: 5293 7005 5293
Size: 1712 5333
SfcI C'TryA_G
Cuts at: 826 1045 2014 2036 2472 3698 3749 4393 5528
Size: 219 969 22 436 1226 51 644 1135
Cuts at: 5528 6209 6400 826
Size: 681 191 1471
Fragments arranged by size:
1471 1226 1135 969 681 644 436 219
191 51 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 2318 2318
Size: 7045
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 7045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7045
SmaI CCC'GGG
Cuts at: 1872 4372 1872
Size: 2500 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3227 3310 3518 3815 4009 5149
Size: 197 926 1284 83 208 297 194 1140
Cuts at: 5149 6050 6312 820
Size: 901 262 1553
Fragments arranged by size:
1553 1284 1140 926 901 297 262 208
197 194 83
SnaBI TAC'GTA
Cuts at: 493 4836 493
Size: 4343 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 4025 4403 1132
Size: 2893 378 3774
Fragments arranged by size:
3774 2893 378
SspI AAT'ATT
Cuts at: 5 52 4969 5
Size: 47 4917 2081
Fragments arranged by size:
4917 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7045
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 2352 2433 2605 2685 3220
Size: 552 939 90 258 81 172 80 535
Cuts at: 3220 3648 4045 4484 513
Size: 428 397 439 3074
Fragments arranged by size:
3074 939 552 535 439 428 397 258
172 90 81 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 2749 2852 3311 3551 3657 3661
Size: 1255 459 710 103 459 240 106 4
Cuts at: 3661 3964 5072 5089 5242 5427 5872 325
Size: 303 1108 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1108 710 459 459 445 303
240 185 153 106 103 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 5291 7003
Size: 274 80 33 51 1443 150 3164 1712
Cuts at: 7003 96
Size: 138
Fragments arranged by size:
3164 1712 1443 274 150 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2663 2695 3238 3270 7005 7037 2663
Size: 32 543 32 3735 32 2671
Fragments arranged by size:
3735 2671 543 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7045
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2488 2591 3211 2488
Size: 103 620 6322
Fragments arranged by size:
6322 620 103
XhoI C'TCGA_G
Cuts at: 1943 4009 1943
Size: 2066 4979
XmaI C'CCGG_G
Cuts at: 1870 4370 1870
Size: 2500 4545
XmnI GAAnn'nnTTC
Cuts at: 4196 5174 4196
Size: 978 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4853 276
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PsrI SacI SacII
SapI SexAI SrfI SwaI