Restriction map for
pFN21AB5045
(Circular) (Six-base) MAPSORT of: pFN21AB5045.seq Check: 6486 from: 1 to: 6664
pFN21AB5045 6664 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2984 3502 2984
Size: 518 6146
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2070 4007 1937
Size: 133 1937 4594
Fragments arranged by size:
4594 1937 133
Acc65I G'GTAC_C
Cuts at: 2238 2874 3985 2238
Size: 636 1111 4917
Fragments arranged by size:
4917 1111 636
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4727 6111 6379 1632
Size: 3095 1384 268 1917
Fragments arranged by size:
3095 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3119 820
Size: 197 2102 4365
Fragments arranged by size:
4365 2102 197
AflIII A'CryG_T
Cuts at: 1203 5563 6540 1203
Size: 4360 977 1327
Fragments arranged by size:
4360 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 3263 1166
Size: 2097 4567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6664
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 6632
AlwNI CAG_nnn'CTG
Cuts at: 3260 4407 5979 3260
Size: 1147 1572 3945
Fragments arranged by size:
3945 1572 1147
ApaI G_GGCC'C
Cuts at: 2133 3853 2133
Size: 1720 4944
ApaLI G'TGCA_C
Cuts at: 2030 2185 4721 5877 2030
Size: 155 2536 1156 2817
Fragments arranged by size:
2817 2536 1156 155
ApoI r'AATT_y
Cuts at: 1493 3973 4199 4351 6460 1493
Size: 2480 226 152 2109 1697
Fragments arranged by size:
2480 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 3081 3989 1870
Size: 73 1138 908 4545
Fragments arranged by size:
4545 1138 908 73
BamHI G'GATC_C
Cuts at: 3994 4361 3994
Size: 367 6297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 2512 2874 3224 3663
Size: 325 217 627 451 274 362 350 439
Cuts at: 3663 3985 5440 618
Size: 322 1455 1842
Fragments arranged by size:
1842 1455 627 451 439 362 350 325
322 274 217
BanII G_rGCy'C
Cuts at: 1391 2133 2460 2547 2732 3853 3983 1391
Size: 742 327 87 185 1121 130 4072
Fragments arranged by size:
4072 1121 742 327 185 130 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3581 3634 3729 928
Size: 971 1682 53 95 3863
Fragments arranged by size:
3863 1682 971 95 53
BbvCI CC'TCA_GC
Cuts at: 2122 2870 2122
Size: 748 5916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4855 4889 1903
Size: 34 2918 34 3678
Fragments arranged by size:
3678 2918 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 5766 4556
Size: 1210 5454
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BfrBI ATG'CAT
Cuts at: 3276 3276
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5275 136
Size: 107 122 71 631 605 3603 1525
Fragments arranged by size:
3603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3393 6659 3393
Size: 3266 3398
BlpI GC'TnA_GC
Cuts at: 3179 3336 4081 3179
Size: 157 745 5762
Fragments arranged by size:
5762 745 157
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2133 2189 2517 3507 3853 4725 5881
Size: 244 99 56 328 990 346 872 1156
Cuts at: 5881 1790
Size: 2573
Fragments arranged by size:
2573 1156 990 872 346 328 244 99
56
BmgBI CAC'GTC
Cuts at: 1318 3441 1318
Size: 2123 4541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4395 5353
Size: 416 53 83 399 24 1853 1125 958
Cuts at: 5353 442
Size: 1753
Fragments arranged by size:
1853 1753 1125 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2939 3623 1056
Size: 1883 684 4097
Fragments arranged by size:
4097 1883 684
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2408 2576 2684 2693 5324 1239
Size: 1088 81 168 108 9 2631 2579
Fragments arranged by size:
2631 2579 1088 168 108 81 9
Bpu10I CC'TnA_GC
Cuts at: 2122 2726 2870 2122
Size: 604 144 5916
Fragments arranged by size:
5916 604 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2582 2720 4789 5654 5952 2531
Size: 51 138 2069 865 298 3243
Fragments arranged by size:
3243 2069 865 298 138 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3078 3377 5327 882
Size: 617 1579 299 1950 2219
Fragments arranged by size:
2219 1950 1579 617 299
BsaAI yAC'GTr
Cuts at: 493 1204 3244 4455 493
Size: 711 2040 1211 2702
Fragments arranged by size:
2702 2040 1211 711
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3767 5097 5495 5769 5916 6327 3767
Size: 1330 398 274 147 411 4104
Fragments arranged by size:
4104 1330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 3026 3056 3745
Size: 30 165 30 726 30 867 30 689
Cuts at: 3745 3775 1178
Size: 30 4067
Fragments arranged by size:
4067 867 726 689 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 1186
Size: 1495 5169
BseYI C'CCAG_C
Cuts at: 3730 5867 6363 3730
Size: 2137 496 4031
Fragments arranged by size:
4031 2137 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2121 2179 3869 1163
Size: 573 385 58 1690 3958
Fragments arranged by size:
3958 1690 573 385 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2189 2460 2547 2732 3056 3180 3332 3486
Size: 155 271 87 185 324 124 152 154
Cuts at: 3486 4725 4810 5881 2034
Size: 1239 85 1071 2817
Fragments arranged by size:
2817 1239 1071 324 271 185 155 154
152 124 87 85
BsmI GAATG_Cn'
Cuts at: 2053 2788 4175 4268 2053
Size: 735 1387 93 4449
Fragments arranged by size:
4449 1387 735 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2133 2189 2460 2517 2547 2732
Size: 399 244 99 56 271 57 30 185
Cuts at: 2732 3056 3180 3332 3486 3507 3853 3983 4725
Size: 324 124 152 154 21 346 130 742
Cuts at: 4725 4810 5881 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 324 271 244
185 154 152 130 124 99 85 57
56 30 21
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 2751 3570 3808 4553 6283 2751
Size: 819 238 745 1730 3132
Fragments arranged by size:
3132 1730 819 745 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2011 2984 3502 4019 844
Size: 1167 973 518 517 3489
Fragments arranged by size:
3489 1167 973 518 517
BsrBI CCG'CTC
Cuts at: 1394 1823 2779 4551 1394
Size: 429 956 1772 3507
Fragments arranged by size:
3507 1772 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5159 5333 65
Size: 1183 3911 174 1396
Fragments arranged by size:
3911 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3457 5308 5495 1700
Size: 110 1647 1851 187 2869
Fragments arranged by size:
2869 1851 1647 187 110
BsrGI T'GTAC_A
Cuts at: 96 3267 96
Size: 3171 3493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 2155 3023 4724 5736 2155
Size: 868 1701 1012 3083
Fragments arranged by size:
3083 1701 1012 868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6664
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 3280 3449 1184
Size: 2096 169 4399
Fragments arranged by size:
4399 2096 169
BstXI CCAn_nnnn'nTGG
Cuts at: 3514 3514
Size: 6664
BstYI r'GATC_y
Cuts at: 1972 3204 3393 3994 4361 4746 4763 6215 6301
Size: 1232 189 601 367 385 17 1452 86
Cuts at: 6301 6313 6659 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1232 601 385 367 346 189
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3402 3402
Size: 6664
BtgI C'CryG_G
Cuts at: 513 1065 2719 3387 3477 513
Size: 552 1654 668 90 3700
Fragments arranged by size:
3700 1654 668 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2092 487
Size: 683 56 866 5059
Fragments arranged by size:
5059 866 683 56
BtsI GCAGTG_nn'
Cuts at: 3615 4180 4974 4994 3615
Size: 565 794 20 5285
Fragments arranged by size:
5285 794 565 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2888 2923 529
Size: 35 2324 35 4270
Fragments arranged by size:
4270 2324 35 35
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2983 4111
Size: 54 1425 50 275 18 540 613 1128
Cuts at: 4111 5000 6629 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 613 540 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2845 4594 1353
Size: 1492 1749 3423
Fragments arranged by size:
3423 1749 1492
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 5251 5625 5771 387
Size: 1064 138 690 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 690 374 146 138
EcoICRI GAG'CTC
Cuts at: 2458 2545 2730 2458
Size: 87 185 6392
Fragments arranged by size:
6392 185 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2408 2576 2684 2693 4727 5324
Size: 393 695 81 168 108 9 2034 597
Cuts at: 5324 6111 6379 1239
Size: 787 268 1524
Fragments arranged by size:
2034 1524 787 695 597 393 268 168
108 81 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 3026 2204
Size: 822 5842
EcoO109I rG'GnC_Cy
Cuts at: 2130 2197 2209 2311 2355 2427 3849 2130
Size: 67 12 102 44 72 1422 4945
Fragments arranged by size:
4945 1422 102 72 67 44 12
EcoRI G'AATT_C
Cuts at: 3973 4351 3973
Size: 378 6286
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 5811 1678
Size: 4133 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 5305 5337 5379
Size: 32 135 32 1454 32 2247 32 42
Cuts at: 5379 5411 1373
Size: 32 2626
Fragments arranged by size:
2626 2247 1454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4008 4254 669
Size: 988 281 2070 246 3079
Fragments arranged by size:
3079 2070 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6664
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KpnI G_GTAC'C
Cuts at: 2242 2878 3989 2242
Size: 636 1111 4917
Fragments arranged by size:
4917 1111 636
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3086 3107 3327 3375 5778 5962 1305
Size: 1781 21 220 48 2403 184 2007
Fragments arranged by size:
2403 2007 1781 220 184 48 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 10
Size: 54 1425 343 540 4302
Fragments arranged by size:
4302 1425 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 3005 3263 3275 4622 4981 5140
Size: 648 1101 738 258 12 1347 359 159
Cuts at: 5140 518
Size: 2042
Fragments arranged by size:
2042 1347 1101 738 648 359 258 159
12
MspA1I CmG'CkG
Cuts at: 1606 2869 2988 3673 4080 4757 5905 6150 1606
Size: 1263 119 685 407 677 1148 245 2120
Fragments arranged by size:
2120 1263 1148 685 677 407 245 119
NaeI GCC'GGC
Cuts at: 3459 3459
Size: 6664
NcoI C'CATG_G
Cuts at: 513 1065 2719 513
Size: 552 1654 4458
Fragments arranged by size:
4458 1654 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NgoMIV G'CCGG_C
Cuts at: 3457 3457
Size: 6664
NheI G'CTAG_C
Cuts at: 1052 2935 3619 1052
Size: 1883 684 4097
Fragments arranged by size:
4097 1883 684
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NsiI A_TGCA'T
Cuts at: 3278 3278
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 2620 2638 3010 3276 4022 5567 1132
Size: 97 1391 18 372 266 746 1545 2229
Fragments arranged by size:
2229 1545 1391 746 372 266 97 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6664
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2305 3845 1108
Size: 1197 1540 3927
Fragments arranged by size:
3927 1540 1197
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PmlI CAC'GTG
Cuts at: 3244 3244
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 2161 2573 2605 4733 4765 6246 6278 2129
Size: 32 412 32 2128 32 1481 32 2515
Fragments arranged by size:
2515 2128 1481 412 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2311 2209
Size: 102 6562
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PspOMI G'GGCC_C
Cuts at: 2129 3849 2129
Size: 1720 4944
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 6632
PstI C_TGCA'G
Cuts at: 830 2319 4016 830
Size: 1489 1697 3478
Fragments arranged by size:
3478 1697 1489
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
SacI G_AGCT'C
Cuts at: 2460 2547 2732 2460
Size: 87 185 6392
Fragments arranged by size:
6392 185 87
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 4912 6624 4912
Size: 1712 4952
SexAI A'CCwGG_T
Cuts at: 2877 2877
Size: 6664
SfcI C'TryA_G
Cuts at: 826 1045 2315 3355 4012 5147 5828 6019 826
Size: 219 1270 1040 657 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 1040 681 657 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 3991 1872
Size: 2119 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 2597 2735 3081 3119 4768
Size: 197 926 603 51 138 346 38 1649
Cuts at: 4768 5669 5931 820
Size: 901 262 1553
Fragments arranged by size:
1649 1553 926 901 603 346 262 197
138 51 38
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 2707 3041 1517
Size: 1190 334 5140
Fragments arranged by size:
5140 1190 334
StyI C'CwwG_G
Cuts at: 513 1065 2719 2906 3200 3936 4103 513
Size: 552 1654 187 294 736 167 3074
Fragments arranged by size:
3074 1654 736 552 294 187 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3213 4691 4708 4861 5046 5491 325
Size: 1255 1633 1478 17 153 185 445 1498
Fragments arranged by size:
1633 1498 1478 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3267 3420 3677
Size: 274 80 33 51 1443 1290 153 257
Cuts at: 3677 4910 6622 96
Size: 1233 1712 138
Fragments arranged by size:
1712 1443 1290 1233 274 257 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2083 2115 6624 6656 2083
Size: 32 4509 32 2091
Fragments arranged by size:
4509 2091 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3376 3376
Size: 6664
XhoI C'TCGA_G
Cuts at: 1943 3081 1943
Size: 1138 5526
XmaI C'CCGG_G
Cuts at: 1870 3989 1870
Size: 2119 4545
XmnI GAAnn'nnTTC
Cuts at: 2690 4793 2690
Size: 2103 4561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I
DraIII EcoRV FalI FseI FspAI KasI NarI NruI
PacI PfoI PspXI PvuII RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI