Restriction map for
pFN21AB5030
(Circular) (Six-base) MAPSORT of: pFN21AB5030.seq Check: 70 from: 1 to: 5557
pFN21AB5030 5557 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2346 2346
Size: 5557
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 2900 1937
Size: 262 701 4594
Fragments arranged by size:
4594 701 262
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3620 5004 5272 1632
Size: 1988 1384 268 1917
Fragments arranged by size:
1988 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 2145 4456 5433 1203
Size: 942 2311 977 1327
Fragments arranged by size:
2311 1327 977 942
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 1184
Size: 1014 4543
AlwNI CAG_nnn'CTG
Cuts at: 3300 4872 3300
Size: 1572 3985
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2483 2866 3092 3244 5353 1493
Size: 990 383 226 152 2109 1697
Fragments arranged by size:
2109 1697 990 383 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2038 2882 1870
Size: 73 95 844 4545
Fragments arranged by size:
4545 844 95 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2471 2878 4333 618
Size: 325 217 627 387 297 407 1455 1842
Fragments arranged by size:
1842 1455 627 407 387 325 297 217
BanII G_rGCy'C
Cuts at: 1391 2876 1391
Size: 1485 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 928
Size: 971 702 3884
Fragments arranged by size:
3884 971 702
BbvCI CC'TCA_GC
Cuts at: 2724 2724
Size: 5557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2044 2078 3748 3782 1903
Size: 34 107 34 1670 34 3678
Fragments arranged by size:
3678 1670 107 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BfrBI ATG'CAT
Cuts at: 2602 2602
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4168 136
Size: 107 122 71 631 605 2496 1525
Fragments arranged by size:
2496 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5552 5552
Size: 5557
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 3618 4774 1790
Size: 1828 1156 2573
Fragments arranged by size:
2573 1828 1156
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 4729
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 4246 442
Size: 416 53 83 399 24 1871 958 1753
Fragments arranged by size:
1871 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 4217 1239
Size: 1052 1926 2579
Fragments arranged by size:
2579 1926 1052
Bpu10I CC'TnA_GC
Cuts at: 2724 2724
Size: 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 3682 4547 4845 2570
Size: 1112 865 298 3282
Fragments arranged by size:
3282 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 4220 882
Size: 617 519 2202 2219
Fragments arranged by size:
2219 2202 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 2539 3348 493
Size: 711 1335 809 2702
Fragments arranged by size:
2702 1335 809 711
BsaBI GATnn'nnATC
Cuts at: 2246 5551 2246
Size: 3305 2252
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3364 3746 4381 275
Size: 53 83 186 936 1831 382 635 1451
Fragments arranged by size:
1831 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5332
Fragments arranged by size:
5332 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4617
BseYI C'CCAG_C
Cuts at: 2030 2138 2512 2667 4760 5256 2030
Size: 108 374 155 2093 496 2331
Fragments arranged by size:
2331 2093 496 374 155 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 1163
Size: 573 825 4159
Fragments arranged by size:
4159 825 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 3768 4796 1540
Size: 275 186 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2538 2559 2691 3618 3703 4774 2538
Size: 21 132 927 85 1071 3321
Fragments arranged by size:
3321 1071 927 132 85 21
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
BsmBI CGTCTCn'nnnn_
Cuts at: 2785 2785
Size: 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2538 2559 2691 2876 3618 3703 4774
Size: 399 748 21 132 185 742 85 1071
Cuts at: 4774 1391
Size: 2174
Fragments arranged by size:
2174 1071 748 742 399 185 132 85
21
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2912 844
Size: 1502 566 3489
Fragments arranged by size:
3489 1502 566
BsrBI CCG'CTC
Cuts at: 1394 1823 2445 3444 1394
Size: 429 622 999 3507
Fragments arranged by size:
3507 999 622 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4201 4388 1700
Size: 110 2391 187 2869
Fragments arranged by size:
2869 2391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2644 2416
Size: 228 5329
BstYI r'GATC_y
Cuts at: 1972 2413 2887 3254 3639 3656 5108 5194 5206
Size: 441 474 367 385 17 1452 86 12
Cuts at: 5206 5552 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 474 441 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2071 2098 2736 513
Size: 552 939 67 27 638 3334
Fragments arranged by size:
3334 939 638 552 67 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3073 3867 3887 2689
Size: 384 794 20 4359
Fragments arranged by size:
4359 794 384 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2384 4564 809
Size: 507 1068 2180 1802
Fragments arranged by size:
2180 1802 1068 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 3004 3893
Size: 54 1425 50 275 18 804 370 889
Cuts at: 3893 5522 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 804 370 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 1537
Size: 275 1192 4090
Fragments arranged by size:
4090 1192 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2743 3487 1353
Size: 1390 744 3423
Fragments arranged by size:
3423 1390 744
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4144 4518 4664 387
Size: 1064 138 435 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 3620 4217 5004 5272 1239
Size: 393 659 1329 597 787 268 1524
Fragments arranged by size:
1524 1329 787 659 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2608 2728 2608
Size: 120 5437
EcoRI G'AATT_C
Cuts at: 2483 2866 3244 2483
Size: 383 378 4796
Fragments arranged by size:
4796 383 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2818 2788
Size: 30 5527
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 2220 4704 1678
Size: 542 2484 2531
Fragments arranged by size:
2531 2484 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373
Size: 32 135 32 2626 32 42 32 2626
Fragments arranged by size:
2626 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4671 4855 1305
Size: 3366 184 2007
Fragments arranged by size:
3366 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 10
Size: 54 1425 343 804 2931
Fragments arranged by size:
2931 1425 804 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 3515 3874 4033 518
Size: 648 1227 1122 359 159 2042
Fragments arranged by size:
2042 1227 1122 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2632 2671 2701 2973 3650 4798 5043
Size: 494 532 39 30 272 677 1148 245
Cuts at: 5043 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 532 494 272 245 39
30
NcoI C'CATG_G
Cuts at: 513 1065 2004 2071 513
Size: 552 939 67 3999
Fragments arranged by size:
3999 939 552 67
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NsiI A_TGCA'T
Cuts at: 2604 2604
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2602 2915 4460 1132
Size: 97 1373 313 1545 2229
Fragments arranged by size:
2229 1545 1373 313 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2701 2704 1108
Size: 1593 3 3961
Fragments arranged by size:
3961 1593 3
PfoI T'CCnGG_A
Cuts at: 2268 2268
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PmlI CAC'GTG
Cuts at: 2539 2539
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 2608
Size: 5557
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2051 2083 2051
Size: 32 5525
PstI C_TGCA'G
Cuts at: 830 2190 2909 830
Size: 1360 719 3478
Fragments arranged by size:
3478 1360 719
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
PvuII CAG'CTG
Cuts at: 2632 2671 2701 2632
Size: 39 30 5488
Fragments arranged by size:
5488 39 30
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 2060 3805 5517 2060
Size: 1745 1712 2100
Fragments arranged by size:
2100 1745 1712
SexAI A'CCwGG_T
Cuts at: 2610 2610
Size: 5557
SfcI C'TryA_G
Cuts at: 826 1045 2186 2905 4040 4721 4912 826
Size: 219 1141 719 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 719 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2040 2884 1872
Size: 168 844 4545
Fragments arranged by size:
4545 844 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3661 4562 4824 820
Size: 197 926 642 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 2382 2409 2996 513
Size: 552 939 67 311 27 587 3074
Fragments arranged by size:
3074 939 587 552 311 67 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3584 3601 3754 3939 4384 325
Size: 1255 2004 17 153 185 445 1498
Fragments arranged by size:
2004 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 3803 5515
Size: 274 80 33 51 1443 81 1745 1712
Cuts at: 5515 96
Size: 138
Fragments arranged by size:
1745 1712 1443 274 138 81 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2416 2416
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2038 2882 1870
Size: 168 844 4545
Fragments arranged by size:
4545 844 168
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI
PspXI RsrII SacI SanDI SapI SfiI SfoI SrfI
SwaI