Restriction map for
pFN21AB5029
(Circular) (Six-base) MAPSORT of: pFN21AB5029.seq Check: 7806 from: 1 to: 6349
pFN21AB5029 6349 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3078 3078
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3561 3692 1937
Size: 1624 131 4594
Fragments arranged by size:
4594 1624 131
Acc65I G'GTAC_C
Cuts at: 3366 3670 3366
Size: 304 6045
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2469 3299 3568 4412 5796 6064 1632
Size: 837 830 269 844 1384 268 1917
Fragments arranged by size:
1917 1384 844 837 830 269 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 2343 5248 6225 1203
Size: 1140 2905 977 1327
Fragments arranged by size:
2905 1327 1140 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2426 1184
Size: 1242 5107
AlwNI CAG_nnn'CTG
Cuts at: 2368 3213 3241 4092 5664 2368
Size: 845 28 851 1572 3053
Fragments arranged by size:
3053 1572 851 845 28
ApaI G_GGCC'C
Cuts at: 2599 2599
Size: 6349
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2475 3674 1870
Size: 73 126 406 1199 4545
Fragments arranged by size:
4545 1199 406 126 73
AvrII C'CTAG_G
Cuts at: 3400 3400
Size: 6349
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3366 3404 3578 3670 5125
Size: 325 217 627 1579 38 174 92 1455
Cuts at: 5125 618
Size: 1842
Fragments arranged by size:
1842 1579 1455 627 325 217 174 92
38
BanII G_rGCy'C
Cuts at: 1391 2599 2898 3668 1391
Size: 1208 299 770 4072
Fragments arranged by size:
4072 1208 770 299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 928
Size: 971 380 4998
Fragments arranged by size:
4998 971 380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2248 4241 5451 2248
Size: 1993 1210 3146
Fragments arranged by size:
3146 1993 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2759 2948 4960
Size: 107 122 71 631 605 1087 189 2012
Cuts at: 4960 136
Size: 1525
Fragments arranged by size:
2012 1525 1087 631 605 189 122 107
71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 2599 4410 5566 1790
Size: 809 1811 1156 2573
Fragments arranged by size:
2573 1811 1156 809
BmgBI CAC'GTC
Cuts at: 1318 3394 1318
Size: 2076 4273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 5038 442
Size: 416 53 83 399 24 2663 958 1753
Fragments arranged by size:
2663 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3171 3527 5009 1239
Size: 1932 356 1482 2579
Fragments arranged by size:
2579 1932 1482 356
Bpu10I CC'TnA_GC
Cuts at: 3449 3449
Size: 6349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2886 3007 3329 3515 4474 5339 5637 2886
Size: 121 322 186 959 865 298 3598
Fragments arranged by size:
3598 959 865 322 298 186 121
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5012 882
Size: 617 3513 2219
Fragments arranged by size:
3513 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3373 4140 493
Size: 711 2169 767 2702
Fragments arranged by size:
2702 2169 767 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3141 3232 4156 4538
Size: 53 83 186 936 1608 91 924 382
Cuts at: 4538 5173 275
Size: 635 1451
Fragments arranged by size:
1608 1451 936 924 635 382 186 91
83 53
BsaWI w'CCGG_w
Cuts at: 4782 5180 5454 5601 6012 4782
Size: 398 274 147 411 5119
Fragments arranged by size:
5119 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2957 2987 1178
Size: 30 165 30 1554 30 4540
Fragments arranged by size:
4540 1554 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2173 2240 2653 3373 3524 3617 3626 3629
Size: 987 67 413 720 151 93 9 3
Cuts at: 3629 1186
Size: 3906
Fragments arranged by size:
3906 987 720 413 151 93 67 9
3
BseYI C'CCAG_C
Cuts at: 2049 2976 3408 5552 6048 2049
Size: 927 432 2144 496 2350
Fragments arranged by size:
2350 2144 927 496 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5776
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3135 4410 4495 5566 3135
Size: 1275 85 1071 3918
Fragments arranged by size:
3918 1275 1071 85
BsmI GAATG_Cn'
Cuts at: 2403 3466 3860 3953 2403
Size: 1063 394 93 4799
Fragments arranged by size:
4799 1063 394 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2599 2898 3135 3668 4410 4495 5566
Size: 399 809 299 237 533 742 85 1071
Cuts at: 5566 1391
Size: 2174
Fragments arranged by size:
2174 1071 809 742 533 399 299 237
85
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 2016 3144 4238 5968 2016
Size: 1128 1094 1730 2397
Fragments arranged by size:
2397 1730 1128 1094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2367 3078 3320 3538 3704 844
Size: 1523 711 242 218 166 3489
Fragments arranged by size:
3489 1523 711 242 218 166
BsrBI CCG'CTC
Cuts at: 1394 1823 2784 4236 1394
Size: 429 961 1452 3507
Fragments arranged by size:
3507 1452 961 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3264 4844 5018 65
Size: 1183 2016 1580 174 1396
Fragments arranged by size:
2016 1580 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 2261 1925
Size: 336 6013
BssSI C'ACGA_G
Cuts at: 2635 4409 5421 2635
Size: 1774 1012 3563
Fragments arranged by size:
3563 1774 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 4775
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2819 2819
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2364 3332 3679 4046 4431 4448 5900 5986
Size: 392 968 347 367 385 17 1452 86
Cuts at: 5986 5998 6344 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 968 392 385 367 347 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2044 2044
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 2004 2943 3270 513
Size: 552 939 939 327 3592
Fragments arranged by size:
3592 939 939 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 2651 2987 3865 4659 4679 2651
Size: 336 878 794 20 4321
Fragments arranged by size:
4321 878 794 336 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2702 3119 5356 809
Size: 507 1386 417 2237 1802
Fragments arranged by size:
2237 1802 1386 507 417
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2760 2940 3796
Size: 54 1425 50 275 18 930 180 856
Cuts at: 3796 4685 6314 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 930 889 856 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2488 2837 3603 3618 3630 4279 1353
Size: 1135 349 766 15 12 649 3423
Fragments arranged by size:
3423 1135 766 649 349 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 3171 3299 3527 3568 4412 5009
Size: 393 837 702 128 228 41 844 597
Cuts at: 5009 5796 6064 1239
Size: 787 268 1524
Fragments arranged by size:
1524 844 837 787 702 597 393 268
228 128 41
EcoO109I rG'GnC_Cy
Cuts at: 2054 2595 2596 3535 2054
Size: 541 1 939 4868
Fragments arranged by size:
4868 939 541 1
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 6349
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2447 2479 3246
Size: 32 135 32 455 32 388 32 767
Cuts at: 3246 3278 4990 5022 5064 5096 1373
Size: 32 1712 32 42 32 2626
Fragments arranged by size:
2626 1712 767 455 388 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3226 3693 3939 669
Size: 988 281 1288 467 246 3079
Fragments arranged by size:
3079 1288 988 467 281 246
HindIII A'AGCT_T
Cuts at: 748 3095 3347 748
Size: 2347 252 3750
Fragments arranged by size:
3750 2347 252
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3370 3674 3370
Size: 304 6045
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2318 5463 5647 1305
Size: 1013 3145 184 2007
Fragments arranged by size:
3145 2007 1013 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2762 2942 10
Size: 54 1425 343 930 180 3417
Fragments arranged by size:
3417 1425 930 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2768 3216 3497 4307 4666 4825 518
Size: 648 1602 448 281 810 359 159 2042
Fragments arranged by size:
2042 1602 810 648 448 359 281 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2287 3765 4442 5590 5835 1606
Size: 642 39 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 642 245 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 2943 513
Size: 552 939 939 3919
Fragments arranged by size:
3919 939 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 2347 3707 5252 1132
Size: 97 1118 1360 1545 2229
Fragments arranged by size:
2229 1545 1360 1118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 2343 5248 2343
Size: 2905 3444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 5206
PfoI T'CCnGG_A
Cuts at: 2031 2031
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 3373 3373
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 3535 2054
Size: 1481 4868
PshAI GACnn'nnGTC
Cuts at: 1613 3231 1613
Size: 1618 4731
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 2595 2595
Size: 6349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 6317
PstI C_TGCA'G
Cuts at: 830 2364 2415 3701 830
Size: 1534 51 1286 3478
Fragments arranged by size:
3478 1534 1286 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2248 2248
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SanDI GG'GwC_CC
Cuts at: 2054 2054
Size: 6349
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 2180 4597 6309 2180
Size: 2417 1712 2220
Fragments arranged by size:
2417 2220 1712
SfcI C'TryA_G
Cuts at: 826 1045 2360 2411 3562 3697 4832 5513 5704
Size: 219 1315 51 1151 135 1135 681 191
Cuts at: 5704 826
Size: 1471
Fragments arranged by size:
1471 1315 1151 1135 681 219 191 135
51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2901 2986 3344 3530 4453 5354
Size: 197 926 958 85 358 186 923 901
Cuts at: 5354 5616 820
Size: 262 1553
Fragments arranged by size:
1553 958 926 923 901 358 262 197
186 85
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2004 2943 3400 3788 513
Size: 552 939 939 457 388 3074
Fragments arranged by size:
3074 939 939 552 457 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3422 4376 4393 4546 4731 5176 325
Size: 1255 1842 954 17 153 185 445 1498
Fragments arranged by size:
1842 1498 1255 954 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2178 4595 6307
Size: 274 80 33 51 1443 201 2417 1712
Cuts at: 6307 96
Size: 138
Fragments arranged by size:
2417 1712 1443 274 201 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 2570 2602 2957 2989 3301 3333 6309
Size: 32 525 32 355 32 312 32 2976
Cuts at: 6309 6341 2013
Size: 32 2021
Fragments arranged by size:
2976 2021 525 355 312 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2702 1316
Size: 1386 4963
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2432 2432
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 2403 2420 4478 2403
Size: 17 2058 4274
Fragments arranged by size:
4274 2058 17
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI