Restriction map for
pFN21AB5025
(Circular) (Six-base) MAPSORT of: pFN21AB5025.seq Check: 6623 from: 1 to: 5422
pFN21AB5025 5422 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 2417 3485 4869 5137 1632
Size: 737 48 1068 1384 268 1917
Fragments arranged by size:
1917 1384 1068 737 268 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 2282
Size: 32 5390
AlwNI CAG_nnn'CTG
Cuts at: 3165 4737 3165
Size: 1572 3850
ApaI G_GGCC'C
Cuts at: 2377 2377
Size: 5422
ApaLI G'TGCA_C
Cuts at: 3479 4635 3479
Size: 1156 4266
ApoI r'AATT_y
Cuts at: 1493 2731 2957 3109 5218 1493
Size: 1238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1238 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2123 2747 1870
Size: 73 174 6 624 4545
Fragments arranged by size:
4545 624 174 73 6
BamHI G'GATC_C
Cuts at: 2752 3119 2752
Size: 367 5055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2564 2743 4198 618
Size: 325 217 627 306 471 179 1455 1842
Fragments arranged by size:
1842 1455 627 471 325 306 217 179
BanII G_rGCy'C
Cuts at: 1391 2112 2124 2377 2741 1391
Size: 721 12 253 364 4072
Fragments arranged by size:
4072 721 364 253 12
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 4123
Fragments arranged by size:
4123 971 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2192 2226 3613 3647 1903
Size: 34 255 34 1387 34 3678
Fragments arranged by size:
3678 1387 255 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 4564
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4033 136
Size: 107 122 71 631 605 2361 1525
Fragments arranged by size:
2361 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2168 2180 2441 2637 2839 2168
Size: 12 261 196 202 4751
Fragments arranged by size:
4751 261 202 196 12
Bme1580I G_kGCm'C
Cuts at: 1790 2377 3483 4639 1790
Size: 587 1106 1156 2573
Fragments arranged by size:
2573 1156 1106 587
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4111 442
Size: 416 53 83 399 24 1736 958 1753
Fragments arranged by size:
1753 1736 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2309 2570 4082 1239
Size: 815 255 261 1512 2579
Fragments arranged by size:
2579 1512 815 261 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 3547 4412 4710 2687
Size: 860 865 298 3399
Fragments arranged by size:
3399 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 3229 3611 4246
Size: 53 83 186 936 561 1135 382 635
Cuts at: 4246 275
Size: 1451
Fragments arranged by size:
1451 1135 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2282 2312 1178
Size: 30 165 30 606 30 243 30 4288
Fragments arranged by size:
4288 606 243 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2069 2078 2675 2678 2681 1186
Size: 844 39 9 597 3 3 3927
Fragments arranged by size:
3927 844 597 39 9 3 3
BseYI C'CCAG_C
Cuts at: 2076 2098 2175 2315 2368 4625 5121 2076
Size: 22 77 140 53 2257 496 2377
Fragments arranged by size:
2377 2257 496 140 77 53 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4849
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3633 4661 1540
Size: 275 186 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3483 3568 4639 3483
Size: 85 1071 4266
Fragments arranged by size:
4266 1071 85
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2124 2377 2741 3483 3568 4639
Size: 399 322 12 253 364 742 85 1071
Cuts at: 4639 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 364 322 253 85
12
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 844
Size: 1933 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2650 3309 1394
Size: 429 827 659 3507
Fragments arranged by size:
3507 827 659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2184 4066 4253 1700
Size: 110 230 144 1882 187 2869
Fragments arranged by size:
2869 1882 230 187 144 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5422
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2752 3119 3504 3521 4973 5059 5071 5417
Size: 780 367 385 17 1452 86 12 346
Cuts at: 5417 1972
Size: 1977
Fragments arranged by size:
1977 1452 780 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 2478 513
Size: 552 939 141 333 3457
Fragments arranged by size:
3457 939 552 333 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3732 3752 2938
Size: 794 20 4608
Fragments arranged by size:
4608 794 20
ClaI AT'CG_AT
Cuts at: 1554 2538 1554
Size: 984 4438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2148 2607
Size: 54 1425 50 275 18 177 141 459
Cuts at: 2607 2869 3758 5387 8
Size: 262 889 1629 43
Fragments arranged by size:
1629 1425 889 459 275 262 177 141
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2393 3352 1353
Size: 1040 959 3423
Fragments arranged by size:
3423 1040 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2669 4009 4383 4529 387
Size: 1064 138 1080 1340 374 146 1280
Fragments arranged by size:
1340 1280 1080 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2309 2369 2417 2570 3485 4082
Size: 393 422 255 60 48 153 915 597
Cuts at: 4082 4869 5137 1239
Size: 787 268 1524
Fragments arranged by size:
1524 915 787 597 422 393 268 255
153 60 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2279 2279
Size: 5422
EcoO109I rG'GnC_Cy
Cuts at: 2284 2373 2374 2284
Size: 89 1 5332
Fragments arranged by size:
5332 89 1
EcoRI G'AATT_C
Cuts at: 2731 3109 2731
Size: 378 5044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2593 2728 2461
Size: 132 135 5155
Fragments arranged by size:
5155 135 132
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 2041 2097 2157 2175 2211 2227 2519 4569
Size: 363 56 60 18 36 16 292 2050
Cuts at: 4569 1678
Size: 2531
Fragments arranged by size:
2531 2050 363 292 60 56 36 18
16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373
Size: 32 135 32 2491 32 42 32 2626
Fragments arranged by size:
2626 2491 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 2525 748
Size: 1777 3645
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4536 4720 1305
Size: 3231 184 2007
Fragments arranged by size:
3231 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3423
Fragments arranged by size:
3423 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3380 3739 3898 518
Size: 648 2214 359 159 2042
Fragments arranged by size:
2214 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2179 2480 2838 3515 4663 4908 1606
Size: 474 99 301 358 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 474 358 301 245 99
NaeI GCC'GGC
Cuts at: 2042 2186 2042
Size: 144 5278
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5422
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3931
Fragments arranged by size:
3931 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5422
NgoMIV G'CCGG_C
Cuts at: 2040 2184 2040
Size: 144 5278
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2299 2780 4325 1132
Size: 97 1070 481 1545 2229
Fragments arranged by size:
2229 1545 1070 481 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5422
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2256 2448 2256
Size: 192 5230
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2314 3491 3523 5004 5036 2282
Size: 32 1177 32 1481 32 2668
Fragments arranged by size:
2668 1481 1177 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2284
Size: 5422
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PspOMI G'GGCC_C
Cuts at: 2373 2373
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2231 2496 2774 830
Size: 1401 265 278 3478
Fragments arranged by size:
3478 1401 278 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2080 2179 2080
Size: 99 5323
SacII CC_GC'GG
Cuts at: 2481 2481
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SapI GCTCTTCn'nnn_
Cuts at: 2393 2393
Size: 5422
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 3670 5382 3670
Size: 1712 3710
SfcI C'TryA_G
Cuts at: 826 1045 2227 2492 2770 3905 4586 4777 826
Size: 219 1182 265 278 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 278 265 219 191
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5422
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 3526 4427 4689 820
Size: 197 926 759 824 901 262 1553
Fragments arranged by size:
1553 926 901 824 759 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5422
StyI C'CwwG_G
Cuts at: 513 1065 2004 2586 2861 513
Size: 552 939 582 275 3074
Fragments arranged by size:
3074 939 582 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3668 5380 96
Size: 274 80 33 51 1443 1691 1712 138
Fragments arranged by size:
1712 1691 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 5382 5414 2009
Size: 32 3341 32 2017
Fragments arranged by size:
3341 2017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2017
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI