Restriction map for
pFN21AB5023
(Circular) (Six-base) MAPSORT of: pFN21AB5023.seq Check: 9408 from: 1 to: 5692
pFN21AB5023 5692 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3755 5139 5407 1632
Size: 2123 1384 268 1917
Fragments arranged by size:
2123 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 2063 4421 2063
Size: 2358 3334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2442 2474 2442
Size: 32 5660
AlwNI CAG_nnn'CTG
Cuts at: 2436 3435 5007 2436
Size: 999 1572 3121
Fragments arranged by size:
3121 1572 999
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2048 2837 3001 3227 3379 5488 1493
Size: 555 789 164 226 152 2109 1697
Fragments arranged by size:
2109 1697 789 555 226 164 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 4468 618
Size: 325 217 627 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2422 2587 3011 1391
Size: 1031 165 424 4072
Fragments arranged by size:
4072 1031 424 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2702 928
Size: 971 803 3918
Fragments arranged by size:
3918 971 803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2775 2809 3883 3917 1903
Size: 34 838 34 1074 34 3678
Fragments arranged by size:
3678 1074 838 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BfrBI ATG'CAT
Cuts at: 2135 2255 2135
Size: 120 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2540 3753 4909 1790
Size: 750 1213 1156 2573
Fragments arranged by size:
2573 1213 1156 750
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2831
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 4352 1239
Size: 1230 1883 2579
Fragments arranged by size:
2579 1883 1230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2725 3817 4682 4980 2725
Size: 1092 865 298 3437
Fragments arranged by size:
3437 1092 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2472 2715 2745 1178
Size: 30 165 30 1039 30 243 30 4125
Fragments arranged by size:
4125 1039 243 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2496 1186
Size: 1310 4382
BseYI C'CCAG_C
Cuts at: 2225 4895 5391 2225
Size: 2670 496 2526
Fragments arranged by size:
2670 2526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2422 3753 3838 4909 2422
Size: 1331 85 1071 3205
Fragments arranged by size:
3205 1331 1071 85
BsmI GAATG_Cn'
Cuts at: 2394 3203 3296 2394
Size: 809 93 4790
Fragments arranged by size:
4790 809 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2422 2540 2587 3011 3753 3838 4909
Size: 399 632 118 47 424 742 85 1071
Cuts at: 4909 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 632 424 399 118 85
47
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 2938 3581 5311 2938
Size: 643 1730 3319
Fragments arranged by size:
3319 1730 643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 2772 3752 4764 2772
Size: 980 1012 3700
Fragments arranged by size:
3700 1012 980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2321 794
Size: 1527 4165
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2138 2138
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2781 513
Size: 552 939 147 630 3424
Fragments arranged by size:
3424 939 630 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2538 3208 4002 4022 2538
Size: 670 794 20 4208
Fragments arranged by size:
4208 794 670 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2374 2996 2374
Size: 622 5070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2928 3139 4028
Size: 54 1425 50 275 18 1098 211 889
Cuts at: 4028 5657 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1098 889 275 211 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 5692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 3755 4352 5139 5407 1239
Size: 393 837 1286 597 787 268 1524
Fragments arranged by size:
1524 1286 837 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2898 2898
Size: 5692
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 2240 2272 2373
Size: 32 135 32 477 32 159 32 101
Cuts at: 2373 2405 2409 2441 2890 2922 4333 4365 4407
Size: 32 4 32 449 32 1411 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1411 477 449 159 135 101 42
32 32 32 32 32 32 32 32
32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2146 3036 3282 669
Size: 988 281 208 890 246 3079
Fragments arranged by size:
3079 988 890 281 246 208
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 2467 4806 4990 1305
Size: 919 243 2339 184 2007
Fragments arranged by size:
2339 2007 919 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2136 2958 3650 4009 4168 518
Size: 648 970 822 692 359 159 2042
Fragments arranged by size:
2042 970 822 692 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3108 3785 4933 5178 1606
Size: 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3424
Fragments arranged by size:
3424 939 777 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NsiI A_TGCA'T
Cuts at: 2137 2257 2137
Size: 120 5572
NspI r_CATG'y
Cuts at: 1132 1229 2139 2502 3050 4595 1132
Size: 97 910 363 548 1545 2229
Fragments arranged by size:
2229 1545 910 548 363 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2442 2474 3761 3793 5274 5306 2442
Size: 32 1287 32 1481 32 2828
Fragments arranged by size:
2828 1481 1287 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2706 1613
Size: 1093 4599
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 3040 4175 4856 5047 826
Size: 219 1995 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2704 3796 4697 4959 820
Size: 197 926 761 1092 901 262 1553
Fragments arranged by size:
1553 1092 926 901 761 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 2502 3050 1132
Size: 1370 548 3774
Fragments arranged by size:
3774 1370 548
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2781 2829 3131 513
Size: 552 939 777 48 302 3074
Fragments arranged by size:
3074 939 777 552 302 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2715 2747 5652 5684 2715
Size: 32 2905 32 2723
Fragments arranged by size:
2905 2723 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI