Restriction map for
pFN21AB5021
(Circular) (Six-base) MAPSORT of: pFN21AB5021.seq Check: 3938 from: 1 to: 6193
pFN21AB5021 6193 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2853 3536 1937
Size: 916 683 4594
Fragments arranged by size:
4594 916 683
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2234 2412 3015 3459 4256 5640 5908
Size: 499 103 178 603 444 797 1384 268
Cuts at: 5908 1632
Size: 1917
Fragments arranged by size:
1917 1384 797 603 499 444 268 178
103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2899 5092 6069 1203
Size: 1696 2193 977 1327
Fragments arranged by size:
2193 1696 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 2903 4922 2903
Size: 2019 4174
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2291 2561 3240 3502 3728 3880 5989 1493
Size: 798 270 679 262 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 679 270 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2966 2999 2966
Size: 33 6160
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3039 3218 3514 4969 618
Size: 325 217 627 1252 179 296 1455 1842
Fragments arranged by size:
1842 1455 1252 627 325 296 217 179
BanII G_rGCy'C
Cuts at: 1391 3512 1391
Size: 2121 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2137 3234 3486 928
Size: 971 238 1097 252 3635
Fragments arranged by size:
3635 1097 971 252 238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4384 4418 1903
Size: 34 50 34 2363 34 3678
Fragments arranged by size:
3678 2363 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2325 4085 5295 2325
Size: 1760 1210 3223
Fragments arranged by size:
3223 1760 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BfrBI ATG'CAT
Cuts at: 3446 3446
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 4254 5410 1790
Size: 2464 1156 2573
Fragments arranged by size:
2573 2464 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 4882 442
Size: 416 53 83 399 24 2507 958 1753
Fragments arranged by size:
2507 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4853 1239
Size: 3614 2579
Bpu10I CC'TnA_GC
Cuts at: 2781 2781
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4318 5183 5481 4318
Size: 865 298 5030
Fragments arranged by size:
5030 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4856 882
Size: 617 3357 2219
Fragments arranged by size:
3357 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6193
BseYI C'CCAG_C
Cuts at: 2060 3273 5396 5892 2060
Size: 1213 2123 496 2361
Fragments arranged by size:
2361 2123 1213 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5620
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2646 2787 4254 4339 5410 2646
Size: 141 1467 85 1071 3429
Fragments arranged by size:
3429 1467 1071 141 85
BsmI GAATG_Cn'
Cuts at: 2055 2348 3424 3446 3704 3797 2055
Size: 293 1076 22 258 93 4451
Fragments arranged by size:
4451 1076 293 258 93 22
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2646 2787 3512 4254 4339 5410 1391
Size: 399 856 141 725 742 85 1071 2174
Fragments arranged by size:
2174 1071 856 742 725 399 141 85
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3548 844
Size: 2704 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4837 5024 1700
Size: 110 3027 187 2869
Fragments arranged by size:
3027 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3286 96
Size: 3190 3003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3443 794
Size: 2649 3544
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 3430 3430
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2383 3523 3890 4275 4292 5744 5830 5842
Size: 411 1140 367 385 17 1452 86 12
Cuts at: 5842 6188 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1140 411 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2854 2854
Size: 6193
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4684
Fragments arranged by size:
4684 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 3709 4503 4523 3709
Size: 794 20 5379
Fragments arranged by size:
5379 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 2187 3497 2187
Size: 1310 4883
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3640 4529 6158
Size: 54 1425 50 275 18 1810 889 1629
Cuts at: 6158 8
Size: 43
Fragments arranged by size:
1810 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 4123 1353
Size: 731 2039 3423
Fragments arranged by size:
3423 2039 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2131 2234 2412 3015 3459 4256 4853
Size: 393 499 103 178 603 444 797 597
Cuts at: 4853 5640 5908 1239
Size: 787 268 1524
Fragments arranged by size:
1524 797 787 603 597 499 444 393
268 178 103
EcoRI G'AATT_C
Cuts at: 2291 3502 3880 2291
Size: 1211 378 4604
Fragments arranged by size:
4604 1211 378
EcoRV GAT'ATC
Cuts at: 3340 3340
Size: 6193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 3251 3247
Size: 4 6189
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2889 2921 4834 4866 4908
Size: 32 135 32 1317 32 1913 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2626 1913 1317 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3537 3783 669
Size: 988 281 1599 246 3079
Fragments arranged by size:
3079 1599 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2708 748
Size: 1960 4233
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2656 5307 5491 1305
Size: 1351 2651 184 2007
Fragments arranged by size:
2651 2007 1351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2337 4151 4510 4669 518
Size: 648 1171 1814 359 159 2042
Fragments arranged by size:
2042 1814 1171 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3273 3609 4286 5434 5679 1606
Size: 1667 336 677 1148 245 2120
Fragments arranged by size:
2120 1667 1148 677 336 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4684
Fragments arranged by size:
4684 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NsiI A_TGCA'T
Cuts at: 3448 3448
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2903 3551 5096 1132
Size: 97 1674 648 1545 2229
Fragments arranged by size:
2229 1674 1545 648 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 2899 5092 2899
Size: 2193 4000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 2106 2178 2328 3763 2106
Size: 72 150 1435 4536
Fragments arranged by size:
4536 1435 150 72
PstI C_TGCA'G
Cuts at: 830 3545 830
Size: 2715 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 3273 3273
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 3027 3411 3541 4676 5357 5548 826
Size: 219 1982 384 130 1135 681 191 1471
Fragments arranged by size:
1982 1471 1135 681 384 219 191 130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4297 5198 5460 820
Size: 197 926 2354 901 262 1553
Fragments arranged by size:
2354 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 2235 2878 4117 5
Size: 47 2183 643 1239 2081
Fragments arranged by size:
2183 2081 1239 643 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2022 3043 3222 3632 513
Size: 552 957 1021 179 410 3074
Fragments arranged by size:
3074 1021 957 552 410 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3286 4439 6151
Size: 274 80 33 51 1443 1309 1153 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
1712 1443 1309 1153 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2295 2877 3231 3375 4322 2295
Size: 582 354 144 947 4166
Fragments arranged by size:
4166 947 582 354 144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI