Restriction map for
pFN21AB5020
(Circular) (Six-base) MAPSORT of: pFN21AB5020.seq Check: 3853 from: 1 to: 6370
pFN21AB5020 6370 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3713 1937
Size: 1776 4594
Acc65I G'GTAC_C
Cuts at: 2829 3399 3691 2829
Size: 570 292 5508
Fragments arranged by size:
5508 570 292
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 2543 2594 3214 4433 5817 6085 1632
Size: 821 90 51 620 1219 1384 268 1917
Fragments arranged by size:
1917 1384 1219 821 620 268 90 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6173
AflIII A'CryG_T
Cuts at: 1203 3108 5269 6246 1203
Size: 1905 2161 977 1327
Fragments arranged by size:
2161 1905 1327 977
AgeI A'CCGG_T
Cuts at: 3652 5201 3652
Size: 1549 4821
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6370
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 6338
AlwNI CAG_nnn'CTG
Cuts at: 3205 4113 5685 3205
Size: 908 1572 3890
Fragments arranged by size:
3890 1572 908
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 2257 3679 3905 4057 6166 1493
Size: 764 1422 226 152 2109 1697
Fragments arranged by size:
2109 1697 1422 764 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 3050 3695 1870
Size: 73 864 243 645 4545
Fragments arranged by size:
4545 864 645 243 73
BamHI G'GATC_C
Cuts at: 3700 4067 3700
Size: 367 6003
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2829 3399 3691 5146
Size: 325 217 627 360 682 570 292 1455
Cuts at: 5146 618
Size: 1842
Fragments arranged by size:
1842 1455 682 627 570 360 325 292
217
BanII G_rGCy'C
Cuts at: 1391 2774 3312 3689 1391
Size: 1383 538 377 4072
Fragments arranged by size:
4072 1383 538 377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 928
Size: 971 118 5281
Fragments arranged by size:
5281 971 118
BbvCI CC'TCA_GC
Cuts at: 3183 3317 3183
Size: 134 6236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4561 4595 1903
Size: 34 2624 34 3678
Fragments arranged by size:
3678 2624 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2187 4262 5472 2187
Size: 2075 1210 3085
Fragments arranged by size:
3085 2075 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6370
BfrBI ATG'CAT
Cuts at: 3262 3262
Size: 6370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4981 136
Size: 107 122 71 631 605 3309 1525
Fragments arranged by size:
3309 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2496 6365 2496
Size: 3869 2501
BlpI GC'TnA_GC
Cuts at: 3787 3787
Size: 6370
Bme1580I G_kGCm'C
Cuts at: 1790 3576 4431 5587 1790
Size: 1786 855 1156 2573
Fragments arranged by size:
2573 1786 1156 855
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6370
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2182 2529 2984
Size: 416 53 83 399 24 765 347 455
Cuts at: 2984 3019 3134 4101 5059 442
Size: 35 115 967 958 1753
Fragments arranged by size:
1753 967 958 765 455 416 399 347
115 83 53 35 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2663 5030 1239
Size: 1067 357 2367 2579
Fragments arranged by size:
2579 2367 1067 357
Bpu10I CC'TnA_GC
Cuts at: 2339 3183 3317 2339
Size: 844 134 5392
Fragments arranged by size:
5392 844 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2970 4495 5360 5658 2970
Size: 1525 865 298 3682
Fragments arranged by size:
3682 1525 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 3664 5033 882
Size: 617 726 1439 1369 2219
Fragments arranged by size:
2219 1439 1369 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 2728 4161 493
Size: 711 1524 1433 2702
Fragments arranged by size:
2702 1524 1433 711
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 4177 4559 5194
Size: 53 83 186 936 534 2110 382 635
Cuts at: 5194 275
Size: 1451
Fragments arranged by size:
2110 1451 936 635 534 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2654 3652 4803 5201 5475 5622 6033 2654
Size: 998 1151 398 274 147 411 2991
Fragments arranged by size:
2991 1151 998 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6145
Fragments arranged by size:
6145 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2898 3350 1186
Size: 913 799 452 4206
Fragments arranged by size:
4206 913 799 452
BseYI C'CCAG_C
Cuts at: 2415 2767 3311 3516 5573 6069 2415
Size: 352 544 205 2057 496 2716
Fragments arranged by size:
2716 2057 544 496 352 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3377 1163
Size: 573 1641 4156
Fragments arranged by size:
4156 1641 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2077 2392 3820 4581 5609
Size: 275 186 21 55 315 1428 761 1028
Cuts at: 5609 1540
Size: 2301
Fragments arranged by size:
2301 1428 1028 761 315 275 186 55
21
BsiHKAI G_wGCw'C
Cuts at: 2228 3501 4431 4516 5587 2228
Size: 1273 930 85 1071 3011
Fragments arranged by size:
3011 1273 1071 930 85
BsmI GAATG_Cn'
Cuts at: 3881 3974 3881
Size: 93 6277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2228 2774 3312 3501 3576 3689 4431
Size: 399 438 546 538 189 75 113 742
Cuts at: 4431 4516 5587 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 538 438 399 189
113 85 75
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 3725 844
Size: 2002 879 3489
Fragments arranged by size:
3489 2002 879
BsrBI CCG'CTC
Cuts at: 1394 1823 4257 1394
Size: 429 2434 3507
Fragments arranged by size:
3507 2434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4865 5039 65
Size: 1183 3617 174 1396
Fragments arranged by size:
3617 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3652 5014 5201 1700
Size: 110 1842 1362 187 2869
Fragments arranged by size:
2869 1842 1362 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6370
BssHII G'CGCG_C
Cuts at: 1925 2014 1925
Size: 89 6281
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2223 794
Size: 1429 4941
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 2599 2858 2956 1184
Size: 1415 259 98 4598
Fragments arranged by size:
4598 1415 259 98
BstYI r'GATC_y
Cuts at: 1972 2496 2835 3453 3612 3700 4067 4452 4469
Size: 524 339 618 159 88 367 385 17
Cuts at: 4469 5921 6007 6019 6365 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 618 524 385 367 346 339
159 88 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2719 2797 513
Size: 552 1654 78 4086
Fragments arranged by size:
4086 1654 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3255 487
Size: 683 56 2029 3602
Fragments arranged by size:
3602 2029 683 56
BtsI GCAGTG_nn'
Cuts at: 2997 3886 4680 4700 2997
Size: 889 794 20 4667
Fragments arranged by size:
4667 889 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5377 809
Size: 507 4061 1802
Fragments arranged by size:
4061 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2074 3001
Size: 54 1425 50 275 18 189 55 927
Cuts at: 3001 3069 3817 4706 6335 8
Size: 68 748 889 1629 43
Fragments arranged by size:
1629 1425 927 889 748 275 189 68
55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 2074 3817 1537
Size: 275 207 55 1743 4090
Fragments arranged by size:
4090 1743 275 207 55
EarI CTCTTCn'nnn_
Cuts at: 1353 3047 3233 3300 4300 1353
Size: 1694 186 67 1000 3423
Fragments arranged by size:
3423 1694 1000 186 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3497 4957 5331 5477 387
Size: 1064 138 1908 1460 374 146 1280
Fragments arranged by size:
1908 1460 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2453 2543 2594 2663 3214 4433
Size: 393 674 147 90 51 69 551 1219
Cuts at: 4433 5030 5817 6085 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1219 787 674 597 551 393 268
147 90 69 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2612 2612
Size: 6370
EcoO109I rG'GnC_Cy
Cuts at: 2089 2866 3386 2089
Size: 777 520 5073
Fragments arranged by size:
5073 777 520
EcoRI G'AATT_C
Cuts at: 3679 4057 3679
Size: 378 5992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 6370
FspI TGC'GCA
Cuts at: 4876 4876
Size: 6370
HaeII r_GCGC'y
Cuts at: 1678 2051 2099 5517 1678
Size: 373 48 3418 2531
Fragments arranged by size:
3418 2531 373 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2827 2859 3438 3470 5011
Size: 32 135 32 1255 32 579 32 1541
Cuts at: 5011 5043 5085 5117 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1541 1255 579 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3714 3960 669
Size: 988 281 1776 246 3079
Fragments arranged by size:
3079 1776 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3439 748
Size: 2691 3679
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 2833 3403 3695 2833
Size: 570 292 5508
Fragments arranged by size:
5508 570 292
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3191 5484 5668 1305
Size: 1886 2293 184 2007
Fragments arranged by size:
2293 2007 1886 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3003 3071 10
Size: 54 1425 343 1171 68 3309
Fragments arranged by size:
3309 1425 1171 343 68 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 3023 4328 4687 4846 518
Size: 648 1770 87 1305 359 159 2042
Fragments arranged by size:
2042 1770 1305 648 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2057 2419 3786 4463 5611 5856 1606
Size: 451 362 1367 677 1148 245 2120
Fragments arranged by size:
2120 1367 1148 677 451 362 245
NcoI C'CATG_G
Cuts at: 513 1065 2719 2797 513
Size: 552 1654 78 4086
Fragments arranged by size:
4086 1654 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NsiI A_TGCA'T
Cuts at: 3264 3264
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 3022 3112 3728 5273 1132
Size: 97 1793 90 616 1545 2229
Fragments arranged by size:
2229 1793 1545 616 97 90
PasI CC'CwG_GG
Cuts at: 1348 2742 1348
Size: 1394 4976
PciI A'CATG_T
Cuts at: 3108 5269 3108
Size: 2161 4209
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2379 3523 1108
Size: 1271 1144 3955
Fragments arranged by size:
3955 1271 1144
PfoI T'CCnGG_A
Cuts at: 2613 2964 2613
Size: 351 6019
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2866 3386 2866
Size: 520 5850
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PstI C_TGCA'G
Cuts at: 830 2219 3722 830
Size: 1389 1503 3478
Fragments arranged by size:
3478 1503 1389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 2419 2419
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SapI GCTCTTCn'nnn_
Cuts at: 3300 3300
Size: 6370
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 4618 6330 4618
Size: 1712 4658
SfcI C'TryA_G
Cuts at: 826 1045 2215 3718 4853 5534 5725 826
Size: 219 1170 1503 1135 681 191 1471
Fragments arranged by size:
1503 1471 1170 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 3652 1700
Size: 1952 4418
SmaI CCC'GGG
Cuts at: 1872 3697 1872
Size: 1825 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2985 4474 5375 5637 820
Size: 197 926 1042 1489 901 262 1553
Fragments arranged by size:
1553 1489 1042 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3022 3728 1132
Size: 1890 706 3774
Fragments arranged by size:
3774 1890 706
SspI AAT'ATT
Cuts at: 5 52 2927 4294 5
Size: 47 2875 1367 2081
Fragments arranged by size:
2875 2081 1367 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6370
StyI C'CwwG_G
Cuts at: 513 1065 2719 2797 3809 513
Size: 552 1654 78 1012 3074
Fragments arranged by size:
3074 1654 1012 552 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4397 4414 4567 4752 5197 325
Size: 1255 2817 17 153 185 445 1498
Fragments arranged by size:
2817 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4616 6328 96
Size: 274 80 33 51 1443 2639 1712 138
Fragments arranged by size:
2639 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6330 6362 6330
Size: 32 6338
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6370
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 3011 2380
Size: 631 5739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 3695 1870
Size: 1825 4545
XmnI GAAnn'nnTTC
Cuts at: 2351 3491 4499 2351
Size: 1140 1008 4222
Fragments arranged by size:
4222 1140 1008
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI