Restriction map for
pFN21AB5019
(Circular) (Six-base) MAPSORT of: pFN21AB5019.seq Check: 5571 from: 1 to: 6220
pFN21AB5019 6220 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2330 2330
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2394 3107 3119 4283 5667 5935 1632
Size: 701 61 713 12 1164 1384 268 1917
Fragments arranged by size:
1917 1384 1164 713 701 268 61 12
AfeI AGC'GCT
Cuts at: 2025 2025
Size: 6220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 5119 6096 1203
Size: 3916 977 1327
Fragments arranged by size:
3916 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 3963 5535 3963
Size: 1572 4648
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2435 2444 2870 3157 3203 3529 3755 3907
Size: 942 9 426 287 46 326 226 152
Cuts at: 3907 6016 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 942 426 326 287 226 152
46 9
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 3545 1870
Size: 73 89 1513 4545
Fragments arranged by size:
4545 1513 89 73
AvrII C'CTAG_G
Cuts at: 2357 2357
Size: 6220
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 4996 618
Size: 325 217 627 1754 1455 1842
Fragments arranged by size:
1842 1754 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 3246 3539 1391
Size: 744 1111 293 4072
Fragments arranged by size:
4072 1111 744 293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2757 928
Size: 971 858 4391
Fragments arranged by size:
4391 971 858
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4411 4445 1903
Size: 34 50 34 2390 34 3678
Fragments arranged by size:
3678 2390 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3272 3637 3272
Size: 365 5855
Bme1580I G_kGCm'C
Cuts at: 1790 2889 4281 5437 1790
Size: 1099 1392 1156 2573
Fragments arranged by size:
2573 1392 1156 1099
BmgBI CAC'GTC
Cuts at: 1318 2083 1318
Size: 765 5455
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2913 1056
Size: 1857 4363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 3046 2524
Size: 522 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2860 2985 3071 3260 3357 4880 1239
Size: 1621 125 86 189 97 1523 2579
Fragments arranged by size:
2579 1621 1523 189 125 97 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2557 4345 5210 5508 2557
Size: 1788 865 298 3269
Fragments arranged by size:
3269 1788 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 3081 6214 3081
Size: 3133 3087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3233 3263 1178
Size: 30 165 30 1830 30 4135
Fragments arranged by size:
4135 1830 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3038 1186
Size: 1852 4368
BseYI C'CCAG_C
Cuts at: 2882 5423 5919 2882
Size: 2541 496 3183
Fragments arranged by size:
3183 2541 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5647
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2960 4281 4366 5437 2960
Size: 1321 85 1071 3743
Fragments arranged by size:
3743 1321 1071 85
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2889 2960 3246 3539 4281 4366
Size: 399 345 754 71 286 293 742 85
Cuts at: 4366 5437 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 754 742 399 345 293 286
85 71
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2330 3575 844
Size: 1486 1245 3489
Fragments arranged by size:
3489 1486 1245
BsrBI CCG'CTC
Cuts at: 1394 1823 4107 1394
Size: 429 2284 3507
Fragments arranged by size:
3507 2284 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3130 4715 4889 65
Size: 1183 1882 1585 174 1396
Fragments arranged by size:
1882 1585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4864 5051 1700
Size: 110 3054 187 2869
Fragments arranged by size:
3054 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 3514 3550 3917 4302 4319 5771 5857 5869
Size: 1542 36 367 385 17 1452 86 12
Cuts at: 5869 6215 1972
Size: 346 1977
Fragments arranged by size:
1977 1542 1452 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2055 513
Size: 552 990 4678
Fragments arranged by size:
4678 990 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2179 2259 2958 3736 4530 4550 2179
Size: 80 699 778 794 20 3849
Fragments arranged by size:
3849 794 778 699 80 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3412 3667 4556
Size: 54 1425 50 275 18 1582 255 889
Cuts at: 4556 6185 8
Size: 1629 43
Fragments arranged by size:
1629 1582 1425 889 275 255 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3083 4150 1353
Size: 1730 1067 3423
Fragments arranged by size:
3423 1730 1067
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2003 4807 5181 5327 387
Size: 1064 138 414 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 414 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2394 2860 2985 3071 3107 3119
Size: 393 701 61 466 125 86 36 12
Cuts at: 3119 3260 3357 4283 4880 5667 5935 1239
Size: 141 97 926 597 787 268 1524
Fragments arranged by size:
1524 926 787 701 597 466 393 268
141 125 97 86 61 36 12
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
EcoRV GAT'ATC
Cuts at: 2303 2303
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2027 2045 5367 1678
Size: 349 18 3322 2531
Fragments arranged by size:
3322 2531 349 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2595 2627 4861
Size: 32 135 32 545 32 446 32 2234
Cuts at: 4861 4893 4935 4967 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2234 545 446 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 2148 2330 2856 5334 5518 1305
Size: 798 45 182 526 2478 184 2007
Fragments arranged by size:
2478 2007 798 526 184 182 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 4178 4537 4696 518
Size: 648 1399 1613 359 159 2042
Fragments arranged by size:
2042 1613 1399 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2072 3636 4313 5461 5706 1606
Size: 451 15 1564 677 1148 245 2120
Fragments arranged by size:
2120 1564 1148 677 451 245 15
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 3337 387
Size: 2950 3270
NheI G'CTAG_C
Cuts at: 1052 2909 1052
Size: 1857 4363
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3578 5123 1132
Size: 97 2349 1545 2229
Fragments arranged by size:
2349 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2838 1108
Size: 1730 4490
PfoI T'CCnGG_A
Cuts at: 2712 2874 2712
Size: 162 6058
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2215 3066 3572 830
Size: 1385 851 506 3478
Fragments arranged by size:
3478 1385 851 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
SacII CC_GC'GG
Cuts at: 2058 2058
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3066 3572 3066
Size: 506 5714
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2211 2469 2904 3062 3568 4703 5384
Size: 219 1166 258 435 158 506 1135 681
Cuts at: 5384 5575 826
Size: 191 1471
Fragments arranged by size:
1471 1166 1135 681 506 435 258 219
191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 2034 3547 1872
Size: 162 1513 4545
Fragments arranged by size:
4545 1513 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2536 4324 5225 5487 820
Size: 197 926 593 1788 901 262 1553
Fragments arranged by size:
1788 1553 926 901 593 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2357 3215 3659 513
Size: 552 1292 858 444 3074
Fragments arranged by size:
3074 1292 858 552 444
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 2032 3545 1870
Size: 162 1513 4545
Fragments arranged by size:
4545 1513 162
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI