Restriction map for
pFN21AB5018
(Circular) (Six-base) MAPSORT of: pFN21AB5018.seq Check: 6256 from: 1 to: 5791
pFN21AB5018 5791 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2359 2728 2359
Size: 369 5422
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2402 3601 278
Size: 53 83 186 936 866 1199 2468
Fragments arranged by size:
2468 1199 936 866 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 2255 3112 2255
Size: 857 4934
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2418 3854 5238 5506 1632
Size: 786 1436 1384 268 1917
Fragments arranged by size:
1917 1436 1384 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 2474 1166
Size: 1308 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2772 1184
Size: 1588 4203
AlwNI CAG_nnn'CTG
Cuts at: 2411 2423 2785 2851 3534 5106 2411
Size: 12 362 66 683 1572 3096
Fragments arranged by size:
3096 1572 683 362 66 12
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 2607 3100 3326 3478 5587 1493
Size: 1114 493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1114 493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 3116 1870
Size: 73 678 495 4545
Fragments arranged by size:
4545 678 495 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2826 2906 3112 4567
Size: 325 217 627 468 571 80 206 1455
Cuts at: 4567 618
Size: 1842
Fragments arranged by size:
1842 1455 627 571 468 325 217 206
80
BanII G_rGCy'C
Cuts at: 1391 2375 2486 2621 2862 3110 1391
Size: 984 111 135 241 248 4072
Fragments arranged by size:
4072 984 248 241 135 111
BbeI G_GCGC'C
Cuts at: 2910 2910
Size: 5791
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 928
Size: 971 564 4256
Fragments arranged by size:
4256 971 564
BbvCI CC'TCA_GC
Cuts at: 2260 2802 2260
Size: 542 5249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2163 5786 2163
Size: 3623 2168
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 3852 5008 1790
Size: 2062 1156 2573
Fragments arranged by size:
2573 2062 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2713 3522 4480
Size: 416 53 83 399 24 1296 809 958
Cuts at: 4480 442
Size: 1753
Fragments arranged by size:
1753 1296 958 809 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 2762 4451 1239
Size: 1478 45 1689 2579
Fragments arranged by size:
2579 1689 1478 45
Bpu10I CC'TnA_GC
Cuts at: 2260 2802 2260
Size: 542 5249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 3916 4781 5079 2188
Size: 1728 865 298 2900
Fragments arranged by size:
2900 1728 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4454 882
Size: 617 2955 2219
Fragments arranged by size:
2955 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2399 2907 2951 3598
Size: 53 83 186 936 866 508 44 647
Cuts at: 3598 3980 4615 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 866 647 635 508 382 186
83 53 44
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2501 2531 1178
Size: 30 165 30 1047 30 21 30 4438
Fragments arranged by size:
4438 1047 165 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2933 1186
Size: 1747 4044
BseYI C'CCAG_C
Cuts at: 2785 4994 5490 2785
Size: 2209 496 3086
Fragments arranged by size:
3086 2209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5218
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3852 3937 5008 3852
Size: 85 1071 4635
Fragments arranged by size:
4635 1071 85
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
BsmBI CGTCTCn'nnnn_
Cuts at: 2066 2489 2066
Size: 423 5368
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2375 2486 2621 2862 3110 3852 3937
Size: 399 585 111 135 241 248 742 85
Cuts at: 3937 5008 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 585 399 248 241 135
111 85
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2359 2728 3146 844
Size: 1515 369 418 3489
Fragments arranged by size:
3489 1515 418 369
BsrBI CCG'CTC
Cuts at: 1394 1823 2539 3678 1394
Size: 429 716 1139 3507
Fragments arranged by size:
3507 1139 716 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2246 2668 2823 4435 4622 1700
Size: 110 346 90 422 155 1612 187 2869
Fragments arranged by size:
2869 1612 422 346 187 155 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 2230 1184
Size: 1046 4745
BstYI r'GATC_y
Cuts at: 1972 2052 2163 3121 3488 3873 3890 5342 5428
Size: 80 111 958 367 385 17 1452 86
Cuts at: 5428 5440 5786 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 958 385 367 346 111 86
80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2329 2329
Size: 5791
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2520 2586 2673 513
Size: 552 939 126 390 66 87 3631
Fragments arranged by size:
3631 939 552 390 126 87 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 2202 1554
Size: 648 5143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2248 2547
Size: 54 1425 50 275 18 177 241 299
Cuts at: 2547 2670 3238 4127 5756 8
Size: 123 568 889 1629 43
Fragments arranged by size:
1629 1425 889 568 299 275 241 177
123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2437 3721 1353
Size: 1084 1284 3423
Fragments arranged by size:
3423 1284 1084
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2377 2891 4378 4752 4898 387
Size: 1064 138 788 514 1487 374 146 1280
Fragments arranged by size:
1487 1280 1064 788 514 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2418 2717 2762 3854 4451 5238 5506
Size: 393 786 299 45 1092 597 787 268
Cuts at: 5506 1239
Size: 1524
Fragments arranged by size:
1524 1092 787 786 597 393 299 268
45
EcoO109I rG'GnC_Cy
Cuts at: 2923 2986 2923
Size: 63 5728
EcoRI G'AATT_C
Cuts at: 2607 3100 3478 2607
Size: 493 378 4920
Fragments arranged by size:
4920 493 378
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2910 4938 1678
Size: 1232 2028 2531
Fragments arranged by size:
2531 2028 1232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2130 2162 2399 2431 2450
Size: 32 135 32 558 32 237 32 19
Cuts at: 2450 2482 2501 2526 2533 2558 4432 4464 4506
Size: 32 19 25 7 25 1874 32 42
Cuts at: 4506 4538 1373
Size: 32 2626
Fragments arranged by size:
2626 1874 558 237 135 42 32 32
32 32 32 32 32 25 25 19
19 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KasI G'GCGC_C
Cuts at: 2906 2906
Size: 5791
KpnI G_GTAC'C
Cuts at: 2259 3116 2259
Size: 857 4934
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3969
Fragments arranged by size:
3969 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 2519 2846 2945 3749 4108 4267
Size: 648 1308 45 327 99 804 359 159
Cuts at: 4267 518
Size: 2042
Fragments arranged by size:
2042 1308 804 648 359 327 159 99
45
MspA1I CmG'CkG
Cuts at: 1606 2288 2445 2785 3207 3884 5032 5277 1606
Size: 682 157 340 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 682 677 422 340 245 157
NaeI GCC'GGC
Cuts at: 2158 2248 2670 2825 2158
Size: 90 422 155 5124
Fragments arranged by size:
5124 422 155 90
NarI GG'CG_CC
Cuts at: 2907 2907
Size: 5791
NcoI C'CATG_G
Cuts at: 513 1065 2004 2520 513
Size: 552 939 516 3784
Fragments arranged by size:
3784 939 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NgoMIV G'CCGG_C
Cuts at: 2156 2246 2668 2823 2156
Size: 90 422 155 5124
Fragments arranged by size:
5124 422 155 90
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2338 2734 3149 4694 1132
Size: 97 1109 396 415 1545 2229
Fragments arranged by size:
2229 1545 1109 415 396 97
PasI CC'CwG_GG
Cuts at: 1348 2864 2989 1348
Size: 1516 125 4150
Fragments arranged by size:
4150 1516 125
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2785 1108
Size: 1677 4114
PfoI T'CCnGG_A
Cuts at: 2643 2643
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PstI C_TGCA'G
Cuts at: 830 2037 3143 830
Size: 1207 1106 3478
Fragments arranged by size:
3478 1207 1106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 2288 2785 2288
Size: 497 5294
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 2033 2970 3139 4274 4955 5146 826
Size: 219 988 937 169 1135 681 191 1471
Fragments arranged by size:
1471 1135 988 937 681 219 191 169
SfoI GGC'GCC
Cuts at: 2908 2908
Size: 5791
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2167 3895 4796 5058 820
Size: 197 926 224 1728 901 262 1553
Fragments arranged by size:
1728 1553 926 901 262 224 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 2276 3020 1517
Size: 759 744 4288
Fragments arranged by size:
4288 759 744
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 3230 513
Size: 552 939 516 710 3074
Fragments arranged by size:
3074 939 710 552 516
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2296 3818 3835 3988 4173 4618 325
Size: 1255 716 1522 17 153 185 445 1498
Fragments arranged by size:
1522 1498 1255 716 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4037 5749 96
Size: 274 80 33 51 1443 2060 1712 138
Fragments arranged by size:
2060 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 5751 5783 2593
Size: 32 3126 32 2601
Fragments arranged by size:
3126 2601 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2177 1316
Size: 861 4930
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2400 3599 276
Size: 53 83 186 936 866 1199 2468
Fragments arranged by size:
2468 1199 936 866 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI