Restriction map for
pFN21AB5013
(Circular) (Six-base) MAPSORT of: pFN21AB5013.seq Check: 5417 from: 1 to: 5314
pFN21AB5013 5314 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2069 3124 278
Size: 53 83 186 936 533 1055 2468
Fragments arranged by size:
2468 1055 936 533 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2657 1937
Size: 720 4594
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 3441 3814 1563
Size: 1878 373 3063
Fragments arranged by size:
3063 1878 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2083 2162 2165 2275 3377 4761 5029 1632
Size: 451 79 3 110 1102 1384 268 1917
Fragments arranged by size:
1917 1384 1102 451 268 110 79 3
AfeI AGC'GCT
Cuts at: 2210 2210
Size: 5314
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 4213 5190 1203
Size: 3010 977 1327
Fragments arranged by size:
3010 1327 977
AgeI A'CCGG_T
Cuts at: 2424 4145 2424
Size: 1721 3593
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5314
AlwNI CAG_nnn'CTG
Cuts at: 3057 4629 3057
Size: 1572 3742
ApaLI G'TGCA_C
Cuts at: 3371 4527 3371
Size: 1156 4158
ApoI r'AATT_y
Cuts at: 1493 2530 2623 2849 3001 5110 1493
Size: 1037 93 226 152 2109 1697
Fragments arranged by size:
2109 1697 1037 226 152 93
AseI AT'TA_AT
Cuts at: 160 1032 2490 3868 160
Size: 872 1458 1378 1606
Fragments arranged by size:
1606 1458 1378 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 1870
Size: 73 696 4545
Fragments arranged by size:
4545 696 73
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 2635 4090 618
Size: 325 217 627 569 279 1455 1842
Fragments arranged by size:
1842 1455 627 569 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2633 1391
Size: 1242 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 1903
Size: 34 1568 34 3678
Fragments arranged by size:
3678 1568 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4416 3206
Size: 1210 4104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5309 5309
Size: 5314
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 2361 3375 4531 1790
Size: 571 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 571
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 4003 442
Size: 416 53 83 399 24 1628 958 1753
Fragments arranged by size:
1753 1628 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2581 3974 1239
Size: 896 446 1393 2579
Fragments arranged by size:
2579 1393 896 446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3439 4304 4602 3439
Size: 865 298 4151
Fragments arranged by size:
4151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3977 882
Size: 617 2478 2219
Fragments arranged by size:
2478 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3105 493
Size: 711 1901 2702
Fragments arranged by size:
2702 1901 711
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2066 3121 3503
Size: 53 83 186 936 516 17 1055 382
Cuts at: 3503 4138 275
Size: 635 1451
Fragments arranged by size:
1451 1055 936 635 516 382 186 83
53 17
BsaWI w'CCGG_w
Cuts at: 2424 3747 4145 4419 4566 4977 2424
Size: 1323 398 274 147 411 2761
Fragments arranged by size:
2761 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2340 2370 1178
Size: 30 165 30 937 30 4122
Fragments arranged by size:
4122 937 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5314
BseYI C'CCAG_C
Cuts at: 4517 5013 4517
Size: 496 4818
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 1163
Size: 573 360 4381
Fragments arranged by size:
4381 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2764 3525 4553 1540
Size: 275 186 763 761 1028 2301
Fragments arranged by size:
2301 1028 763 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3375 3460 4531 3375
Size: 85 1071 4158
Fragments arranged by size:
4158 1071 85
BsmI GAATG_Cn'
Cuts at: 2825 2918 2825
Size: 93 5221
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2633 3375 3460 4531 1391
Size: 399 571 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 399 272 85
BspEI T'CCGG_A
Cuts at: 4977 4977
Size: 5314
BspHI T'CATG_A
Cuts at: 3203 4933 3203
Size: 1730 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 844
Size: 1825 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 1394
Size: 429 1378 3507
Fragments arranged by size:
3507 1378 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3809 3983 65
Size: 1183 2561 174 1396
Fragments arranged by size:
2561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2353 2424 3958 4145 1700
Size: 110 543 71 1534 187 2869
Fragments arranged by size:
2869 1534 543 187 110 71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5314
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5314
BssSI C'ACGA_G
Cuts at: 3374 4386 3374
Size: 1012 4302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5314
BstBI TT'CG_AA
Cuts at: 4118 4118
Size: 5314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2644 3011 3396 3413 4865 4951 4963 5309
Size: 672 367 385 17 1452 86 12 346
Cuts at: 5309 1972
Size: 1977
Fragments arranged by size:
1977 1452 672 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 3709
Fragments arranged by size:
3709 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4575
Fragments arranged by size:
4575 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2409 2830 3624 3644 2061
Size: 348 421 794 20 3731
Fragments arranged by size:
3731 794 421 348 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4321 809
Size: 507 3005 1802
Fragments arranged by size:
3005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2761 3650
Size: 54 1425 50 275 18 543 388 889
Cuts at: 3650 5279 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 543 388 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2761 1537
Size: 275 949 4090
Fragments arranged by size:
4090 949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3244 1353
Size: 1891 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3901 4275 4421 387
Size: 1064 138 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2135 2162 2165 2275 2581 3377
Size: 393 451 52 27 3 110 306 796
Cuts at: 3377 3974 4761 5029 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 796 787 597 451 393 306 268
110 52 27 3
EcoO109I rG'GnC_Cy
Cuts at: 2317 2317
Size: 5314
EcoRI G'AATT_C
Cuts at: 2623 3001 2623
Size: 378 4936
EcoRV GAT'ATC
Cuts at: 2545 2545
Size: 5314
FspI TGC'GCA
Cuts at: 3820 3820
Size: 5314
HaeII r_GCGC'y
Cuts at: 1678 2212 4461 1678
Size: 534 2249 2531
Fragments arranged by size:
2531 2249 534
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 3955 3987 4029
Size: 32 135 32 962 32 1389 32 42
Cuts at: 4029 4061 1373
Size: 32 2626
Fragments arranged by size:
2626 1389 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2913 2913
Size: 5314
MluI A'CGCG_T
Cuts at: 5190 5190
Size: 5314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2435 4428 4612 1305
Size: 1130 1993 184 2007
Fragments arranged by size:
2007 1993 1130 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 2949
Fragments arranged by size:
2949 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 3272 3631 3790 518
Size: 648 1053 1053 359 159 2042
Fragments arranged by size:
2042 1053 1053 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2601 2730 3407 4555 4800 1606
Size: 579 416 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 416 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3823
Fragments arranged by size:
3823 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5314
NotI GC'GGCC_GC
Cuts at: 2761 2761
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2672 4217 1132
Size: 97 1443 1545 2229
Fragments arranged by size:
2229 1545 1443 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5314
PciI A'CATG_T
Cuts at: 4213 4213
Size: 5314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2317 2317
Size: 5314
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PstI C_TGCA'G
Cuts at: 830 2666 830
Size: 1836 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
PvuII CAG'CTG
Cuts at: 2185 2601 2185
Size: 416 4898
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SbfI CC_TGCA'GG
Cuts at: 2666 2666
Size: 5314
ScaI AGT'ACT
Cuts at: 3562 5274 3562
Size: 1712 3602
SfcI C'TryA_G
Cuts at: 826 1045 2662 3797 4478 4669 826
Size: 219 1617 1135 681 191 1471
Fragments arranged by size:
1617 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2641 1872
Size: 769 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3418 4319 4581 820
Size: 197 926 1475 901 262 1553
Fragments arranged by size:
1553 1475 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5314
SphI G_CATG'C
Cuts at: 1132 2672 1132
Size: 1540 3774
SspI AAT'ATT
Cuts at: 5 52 2488 3238 5
Size: 47 2436 750 2081
Fragments arranged by size:
2436 2081 750 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2396 2563 2753 513
Size: 552 939 392 167 190 3074
Fragments arranged by size:
3074 939 552 392 190 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2576 3341 3358 3511 3696 4141 325
Size: 1255 996 765 17 153 185 445 1498
Fragments arranged by size:
1498 1255 996 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3560 5272 96
Size: 274 80 33 51 1443 1583 1712 138
Fragments arranged by size:
1712 1583 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2099 2131 2340 2372 5274 5306 2099
Size: 32 209 32 2902 32 2107
Fragments arranged by size:
2902 2107 209 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5314
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2125
Size: 5314
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5314
XmaI C'CCGG_G
Cuts at: 1870 2639 1870
Size: 769 4545
XmnI GAAnn'nnTTC
Cuts at: 2444 3443 2444
Size: 999 4315
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2067 3122 276
Size: 53 83 186 936 533 1055 2468
Fragments arranged by size:
2468 1055 936 533 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI