Restriction map for
pFN21AB5012
(Circular) (Six-base) MAPSORT of: pFN21AB5012.seq Check: 1941 from: 1 to: 6334
pFN21AB5012 6334 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2199 2808 3677 1937
Size: 262 609 869 4594
Fragments arranged by size:
4594 869 609 262
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3062 4397 5781 6049 1632
Size: 413 1017 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 1017 413 268
AfeI AGC'GCT
Cuts at: 3413 3413
Size: 6334
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 2680 3604 4077 5649 2680
Size: 924 473 1572 3365
Fragments arranged by size:
3365 1572 924 473
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 2372 3643 3869 4021 6130 1493
Size: 879 1271 226 152 2109 1697
Fragments arranged by size:
2109 1697 1271 879 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 3659 1870
Size: 73 1716 4545
Fragments arranged by size:
4545 1716 73
BamHI G'GATC_C
Cuts at: 2104 3664 4031 2104
Size: 1560 367 4407
Fragments arranged by size:
4407 1560 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3655 5110 618
Size: 325 217 627 1868 1455 1842
Fragments arranged by size:
1868 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3653 1391
Size: 2262 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2892 928
Size: 971 993 4370
Fragments arranged by size:
4370 993 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2179 2213 3192 3226 4525 4559 1903
Size: 34 242 34 979 34 1299 34 3678
Fragments arranged by size:
3678 1299 979 242 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4226 5436 3275
Size: 951 1210 4173
Fragments arranged by size:
4173 1210 951
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 4945 136
Size: 107 122 71 631 605 688 2585 1525
Fragments arranged by size:
2585 1525 688 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2653 6329 2653
Size: 3676 2658
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 2507 4395 5551 1790
Size: 717 1888 1156 2573
Fragments arranged by size:
2573 1888 1156 717
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2224 4065 5023
Size: 416 53 83 399 24 807 1841 958
Cuts at: 5023 442
Size: 1753
Fragments arranged by size:
1841 1753 958 807 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3248 3293 3364 4994 1239
Size: 2009 45 71 1630 2579
Fragments arranged by size:
2579 2009 1630 71 45
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3230 4459 5324 5622 2996
Size: 234 1229 865 298 3708
Fragments arranged by size:
3708 1229 865 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2621 4997 882
Size: 617 1122 2376 2219
Fragments arranged by size:
2376 2219 1122 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 4125 493
Size: 711 915 2006 2702
Fragments arranged by size:
2702 2006 915 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4523 5158 275
Size: 53 83 186 936 2608 382 635 1451
Fragments arranged by size:
2608 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 3362 3392 1178
Size: 30 165 30 939 30 990 30 4120
Fragments arranged by size:
4120 990 939 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2306 2594 3104 1186
Size: 1120 288 510 4416
Fragments arranged by size:
4416 1120 510 288
BseYI C'CCAG_C
Cuts at: 2695 3508 5537 6033 2695
Size: 813 2029 496 2996
Fragments arranged by size:
2996 2029 813 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3509 1163
Size: 573 1773 3988
Fragments arranged by size:
3988 1773 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2994 3012 4395 4480 5551 2994
Size: 18 1383 85 1071 3777
Fragments arranged by size:
3777 1383 1071 85 18
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2507 2994 3012 3653 4395 4480 5551
Size: 399 717 487 18 641 742 85 1071
Cuts at: 5551 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 717 641 487 399 85
18
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2376 2529 3498 4223 5953 2376
Size: 153 969 725 1730 2757
Fragments arranged by size:
2757 1730 969 725 153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2002 3689 844
Size: 1158 1687 3489
Fragments arranged by size:
3489 1687 1158
BsrBI CCG'CTC
Cuts at: 1394 1823 3055 4221 1394
Size: 429 1232 1166 3507
Fragments arranged by size:
3507 1232 1166 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3435 4978 5165 1700
Size: 110 1625 1543 187 2869
Fragments arranged by size:
2869 1625 1543 187 110
BsrGI T'GTAC_A
Cuts at: 96 2124 96
Size: 2028 4306
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 2306 4394 5406 2306
Size: 2088 1012 3234
Fragments arranged by size:
3234 2088 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 2764 2866 3226 3346 2764
Size: 102 360 120 5752
Fragments arranged by size:
5752 360 120 102
BstYI r'GATC_y
Cuts at: 1972 2104 2518 2653 3594 3664 4031 4416 4433
Size: 132 414 135 941 70 367 385 17
Cuts at: 4433 5885 5971 5983 6329 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 941 414 385 367 346 135
132 86 70 17 12
BstZ17I GTA'TAC
Cuts at: 2809 2809
Size: 6334
Bsu36I CC'TnA_GG
Cuts at: 3483 3483
Size: 6334
BtgI C'CryG_G
Cuts at: 513 1065 2004 3345 3471 513
Size: 552 939 1341 126 3376
Fragments arranged by size:
3376 1341 939 552 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3338 487
Size: 683 56 2112 3483
Fragments arranged by size:
3483 2112 683 56
BtsI GCAGTG_nn'
Cuts at: 3850 4644 4664 3850
Size: 794 20 5520
Fragments arranged by size:
5520 794 20
ClaI AT'CG_AT
Cuts at: 1554 2064 1554
Size: 510 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3474 3781 4670
Size: 54 1425 50 275 18 1644 307 889
Cuts at: 4670 6299 8
Size: 1629 43
Fragments arranged by size:
1644 1629 1425 889 307 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2580 4264 1353
Size: 1227 1684 3423
Fragments arranged by size:
3423 1684 1227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 3062 3248 3293 3364 4397 4994
Size: 393 413 1017 186 45 71 1033 597
Cuts at: 4994 5781 6049 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1033 1017 787 597 413 393 268
186 71 45
EcoO109I rG'GnC_Cy
Cuts at: 2044 3544 2044
Size: 1500 4834
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2672 2740 2672
Size: 68 6266
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 3368 3415 5481 1678
Size: 1690 47 2066 2531
Fragments arranged by size:
2531 2066 1690 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3362 3394 4975 5007 5049
Size: 32 135 32 1790 32 1581 32 42
Cuts at: 5049 5081 1373
Size: 32 2626
Fragments arranged by size:
2626 1790 1581 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2200 3678 3924 669
Size: 988 281 262 1478 246 3079
Fragments arranged by size:
3079 1478 988 281 262 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 5448 5632 1305
Size: 1089 3054 184 2007
Fragments arranged by size:
3054 2007 1089 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4512
Fragments arranged by size:
4512 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 2864 3350 4292 4651 4810 518
Size: 648 1611 87 486 942 359 159 2042
Fragments arranged by size:
2042 1611 942 648 486 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2804 3238 3271 3311 3384 3750 4427 5575
Size: 1198 434 33 40 73 366 677 1148
Cuts at: 5575 5820 1606
Size: 245 2120
Fragments arranged by size:
2120 1198 1148 677 434 366 245 73
40 33
NcoI C'CATG_G
Cuts at: 513 1065 2004 3345 3471 513
Size: 552 939 1341 126 3376
Fragments arranged by size:
3376 1341 939 552 126
NdeI CA'TA_TG
Cuts at: 387 2911 387
Size: 2524 3810
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2395 3692 5237 1132
Size: 97 1166 1297 1545 2229
Fragments arranged by size:
2229 1545 1297 1166 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 3225 3525 3225
Size: 300 6034
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PmlI CAC'GTG
Cuts at: 2119 2119
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3544 3544
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2693 2725 2693
Size: 32 6302
PstI C_TGCA'G
Cuts at: 830 3686 830
Size: 2856 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 3238 3271 3384 3238
Size: 33 113 6188
Fragments arranged by size:
6188 113 33
SalI G'TCGA_C
Cuts at: 1936 2198 3676 1936
Size: 262 1478 4594
Fragments arranged by size:
4594 1478 262
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 3682 4817 5498 5689 826
Size: 219 2637 1135 681 191 1471
Fragments arranged by size:
2637 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3011 3245 4438 5339 5601 820
Size: 197 926 1068 234 1193 901 262 1553
Fragments arranged by size:
1553 1193 1068 926 901 262 234 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2004 2551 2757 3345 3471 3773 513
Size: 552 939 547 206 588 126 302 3074
Fragments arranged by size:
3074 939 588 552 547 302 206 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4361 4378 4531 4716 5161 325
Size: 1255 2781 17 153 185 445 1498
Fragments arranged by size:
2781 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2949 3429
Size: 274 80 33 51 1443 147 825 480
Cuts at: 3429 4580 6292 96
Size: 1151 1712 138
Fragments arranged by size:
1712 1443 1151 825 480 274 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3579 3611 6294 6326 3579
Size: 32 2683 32 3587
Fragments arranged by size:
3587 2683 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 2248 3670 2248
Size: 1422 4912
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 2384 2655 4463 2384
Size: 271 1808 4255
Fragments arranged by size:
4255 1808 271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI