Restriction map for
pFN21AB5008
(Circular) (Six-base) MAPSORT of: pFN21AB5008.seq Check: 1746 from: 1 to: 5587
pFN21AB5008 5587 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2017 2564 2017
Size: 547 5040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3397 278
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2930 1937
Size: 993 4594
Acc65I G'GTAC_C
Cuts at: 2908 2908
Size: 5587
AclI AA'CG_TT
Cuts at: 1563 3714 4087 1563
Size: 2151 373 3063
Fragments arranged by size:
3063 2151 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3650 5034 5302 1632
Size: 2018 1384 268 1917
Fragments arranged by size:
2018 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5390
AflIII A'CryG_T
Cuts at: 1203 4486 5463 1203
Size: 3283 977 1327
Fragments arranged by size:
3283 1327 977
AgeI A'CCGG_T
Cuts at: 4418 4418
Size: 5587
AhdI GACnn_n'nnGTC
Cuts at: 4316 4316
Size: 5587
AleI CACnn'nnGTG
Cuts at: 1166 2777 1166
Size: 1611 3976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5587
AlwNI CAG_nnn'CTG
Cuts at: 2743 3330 4902 2743
Size: 587 1572 3428
Fragments arranged by size:
3428 1572 587
ApaI G_GGCC'C
Cuts at: 2113 2829 2113
Size: 716 4871
ApaLI G'TGCA_C
Cuts at: 3644 4800 3644
Size: 1156 4431
ApoI r'AATT_y
Cuts at: 1493 2896 3122 3274 5383 1493
Size: 1403 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4141 160
Size: 872 3109 1606
Fragments arranged by size:
3109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
AvaI C'yCGr_G
Cuts at: 1870 1943 2775 2912 1870
Size: 73 832 137 4545
Fragments arranged by size:
4545 832 137 73
BamHI G'GATC_C
Cuts at: 2917 3284 2917
Size: 367 5220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2831 2908 4363 618
Size: 325 217 627 758 286 77 1455 1842
Fragments arranged by size:
1842 1455 758 627 325 286 217 77
BanII G_rGCy'C
Cuts at: 1391 2113 2325 2820 2829 2906 1391
Size: 722 212 495 9 77 4072
Fragments arranged by size:
4072 722 495 212 77 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2501 2535 3778 3812 1903
Size: 34 50 34 480 34 1243 34 3678
Fragments arranged by size:
3678 1243 480 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3479 4689 3479
Size: 1210 4377
BclI T'GATC_A
Cuts at: 1548 2673 1548
Size: 1125 4462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2183 4198 136
Size: 107 122 71 631 605 511 2015 1525
Fragments arranged by size:
2015 1525 631 605 511 122 107 71
BglII A'GATC_T
Cuts at: 5582 5582
Size: 5587
BlpI GC'TnA_GC
Cuts at: 3004 3004
Size: 5587
Bme1580I G_kGCm'C
Cuts at: 1790 2113 2524 2550 2829 3648 4804 1790
Size: 323 411 26 279 819 1156 2573
Fragments arranged by size:
2573 1156 819 411 323 279 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2731 3318 4276
Size: 416 53 83 399 24 1314 587 958
Cuts at: 4276 442
Size: 1753
Fragments arranged by size:
1753 1314 958 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 4247 1239
Size: 933 2075 2579
Fragments arranged by size:
2579 2075 933
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2048 3712 4577 4875 2048
Size: 1664 865 298 2760
Fragments arranged by size:
2760 1664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2236 4250 882
Size: 617 737 2014 2219
Fragments arranged by size:
2219 2014 737 617
BsaAI yAC'GTr
Cuts at: 493 1204 2650 3378 493
Size: 711 1446 728 2702
Fragments arranged by size:
2702 1446 728 711
BsaBI GATnn'nnATC
Cuts at: 5581 5581
Size: 5587
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 2252 3394 3776
Size: 53 83 186 936 681 38 1142 382
Cuts at: 3776 4411 275
Size: 635 1451
Fragments arranged by size:
1451 1142 936 681 635 382 186 83
53 38
BsaWI w'CCGG_w
Cuts at: 2352 4020 4418 4692 4839 5250 2352
Size: 1668 398 274 147 411 2689
Fragments arranged by size:
2689 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5362
Fragments arranged by size:
5362 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5587
BseYI C'CCAG_C
Cuts at: 2840 4790 5286 2840
Size: 1950 496 3141
Fragments arranged by size:
3141 1950 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2644 2654 1163
Size: 573 908 10 4096
Fragments arranged by size:
4096 908 573 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3798 4826 1540
Size: 275 186 1036 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2802 3648 3733 4804 2325
Size: 477 846 85 1071 3108
Fragments arranged by size:
3108 1071 846 477 85
BsmI GAATG_Cn'
Cuts at: 2285 3098 3191 2285
Size: 813 93 4681
Fragments arranged by size:
4681 813 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2325 2524 2550 2802 2820 2829
Size: 399 323 212 199 26 252 18 9
Cuts at: 2829 2906 3648 3733 4804 1391
Size: 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 323 252 212 199
85 77 26 18 9
BspEI T'CCGG_A
Cuts at: 2352 5250 2352
Size: 2898 2689
BspHI T'CATG_A
Cuts at: 3476 5206 3476
Size: 1730 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 2564 2942 844
Size: 1173 547 378 3489
Fragments arranged by size:
3489 1173 547 378
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 3474 1394
Size: 429 326 1325 3507
Fragments arranged by size:
3507 1325 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 4082 4256 65
Size: 1183 2834 174 1396
Fragments arranged by size:
2834 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4231 4418 1700
Size: 110 2421 187 2869
Fragments arranged by size:
2869 2421 187 110
BsrGI T'GTAC_A
Cuts at: 96 2292 2766 96
Size: 2196 474 2917
Fragments arranged by size:
2917 2196 474
BssHII G'CGCG_C
Cuts at: 1925 2040 2042 1925
Size: 115 2 5470
Fragments arranged by size:
5470 115 2
BssSI C'ACGA_G
Cuts at: 3647 4659 3647
Size: 1012 4575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2865 794
Size: 2071 3516
BstBI TT'CG_AA
Cuts at: 2389 4391 2389
Size: 2002 3585
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5587
BstYI r'GATC_y
Cuts at: 1972 2304 2917 3284 3669 3686 5138 5224 5236
Size: 332 613 367 385 17 1452 86 12
Cuts at: 5236 5582 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 613 385 367 346 332 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5035
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4848
Fragments arranged by size:
4848 683 56
BtsI GCAGTG_nn'
Cuts at: 2524 3103 3897 3917 2524
Size: 579 794 20 4194
Fragments arranged by size:
4194 794 579 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2449 2484 529
Size: 35 1885 35 3632
Fragments arranged by size:
3632 1885 35 35
DraI TTT'AAA
Cuts at: 2891 2891
Size: 5587
DraIII CAC_nnn'GTG
Cuts at: 2595 2595
Size: 5587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4594 809
Size: 507 3278 1802
Fragments arranged by size:
3278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552
Size: 54 1425 50 275 18 1204 889 1629
Cuts at: 5552 8
Size: 43
Fragments arranged by size:
1629 1425 1204 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 1537
Size: 275 1222 4090
Fragments arranged by size:
4090 1222 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2386 3517 1353
Size: 1033 1131 3423
Fragments arranged by size:
3423 1131 1033
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4174 4548 4694 387
Size: 1064 138 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 2323
Size: 5587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 3650 4247 5034 5302 1239
Size: 393 540 1478 597 787 268 1524
Fragments arranged by size:
1524 1478 787 597 540 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2109 2110 2825 2109
Size: 1 715 4871
Fragments arranged by size:
4871 715 1
EcoRI G'AATT_C
Cuts at: 2896 3274 2896
Size: 378 5209
FspI TGC'GCA
Cuts at: 4093 4093
Size: 5587
HaeII r_GCGC'y
Cuts at: 1678 4734 1678
Size: 3056 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2485 2517 4228 4260 4302
Size: 32 135 32 913 32 1711 32 42
Cuts at: 4302 4334 1373
Size: 32 2626
Fragments arranged by size:
2626 1711 913 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 2931 3177 669
Size: 988 281 649 344 246 3079
Fragments arranged by size:
3079 988 649 344 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5587
HpaI GTT'AAC
Cuts at: 3177 3177
Size: 5587
KpnI G_GTAC'C
Cuts at: 2912 2912
Size: 5587
MfeI C'AATT_G
Cuts at: 3186 3186
Size: 5587
MluI A'CGCG_T
Cuts at: 5463 5463
Size: 5587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4701 4885 1305
Size: 3396 184 2007
Fragments arranged by size:
3396 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3765
Fragments arranged by size:
3765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2243 2648 2702 2777 2792 3545
Size: 648 1059 18 405 54 75 15 753
Cuts at: 3545 3904 4063 518
Size: 359 159 2042
Fragments arranged by size:
2042 1059 753 648 405 359 159 75
54 18 15
MspA1I CmG'CkG
Cuts at: 1606 3003 3680 4828 5073 1606
Size: 1397 677 1148 245 2120
Fragments arranged by size:
2120 1397 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5035
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5587
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5587
NotI GC'GGCC_GC
Cuts at: 3034 3034
Size: 5587
NspI r_CATG'y
Cuts at: 1132 1229 2945 4490 1132
Size: 97 1716 1545 2229
Fragments arranged by size:
2229 1716 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5587
PciI A'CATG_T
Cuts at: 4486 4486
Size: 5587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 1108
Size: 1635 3952
PmeI GTTT'AAAC
Cuts at: 2891 2891
Size: 5587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 5169 5201 3656
Size: 32 1481 32 4042
Fragments arranged by size:
4042 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5587
PsiI TTA'TAA
Cuts at: 3157 3157
Size: 5587
PspOMI G'GGCC_C
Cuts at: 2109 2825 2109
Size: 716 4871
PstI C_TGCA'G
Cuts at: 830 2519 2939 830
Size: 1689 420 3478
Fragments arranged by size:
3478 1689 420
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5587
SacI G_AGCT'C
Cuts at: 2325 2325
Size: 5587
SalI G'TCGA_C
Cuts at: 1936 2929 1936
Size: 993 4594
SbfI CC_TGCA'GG
Cuts at: 2939 2939
Size: 5587
ScaI AGT'ACT
Cuts at: 3835 5547 3835
Size: 1712 3875
SfcI C'TryA_G
Cuts at: 826 1045 2515 2935 4070 4751 4942 826
Size: 219 1470 420 1135 681 191 1471
Fragments arranged by size:
1471 1470 1135 681 420 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5587
SmaI CCC'GGG
Cuts at: 1872 2777 2914 1872
Size: 905 137 4545
Fragments arranged by size:
4545 905 137
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 3691 4592 4854 820
Size: 197 926 120 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 3378 493
Size: 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5587
SphI G_CATG'C
Cuts at: 1132 2945 1132
Size: 1813 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5
Size: 47 3459 2081
Fragments arranged by size:
3459 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5587
StyI C'CwwG_G
Cuts at: 513 1065 2299 3026 513
Size: 552 1234 727 3074
Fragments arranged by size:
3074 1234 727 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3614 3631 3784 3969 4414 325
Size: 1255 2034 17 153 185 445 1498
Fragments arranged by size:
2034 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 2766 3833
Size: 274 80 33 51 1443 315 474 1067
Cuts at: 3833 5545 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1067 474 315 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5547 5579 5547
Size: 32 5555
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5587
XbaI T'CTAG_A
Cuts at: 2923 2923
Size: 5587
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2842 2842
Size: 5587
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5587
XmaI C'CCGG_G
Cuts at: 1870 2775 2912 1870
Size: 905 137 4545
Fragments arranged by size:
4545 905 137
XmnI GAAnn'nnTTC
Cuts at: 2456 3716 2456
Size: 1260 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3395 276
Size: 53 83 186 936 1861 2468
Fragments arranged by size:
2468 1861 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI