Restriction map for
pFN21AB5006
(Circular) (Six-base) MAPSORT of: pFN21AB5006.seq Check: 7808 from: 1 to: 6082
pFN21AB5006 6082 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2330 2330
Size: 6082
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3425 1937
Size: 1488 4594
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 4145 5529 5797 1632
Size: 494 2019 1384 268 1917
Fragments arranged by size:
2019 1917 1384 494 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 4981 5958 1203
Size: 3778 977 1327
Fragments arranged by size:
3778 1327 977
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 2819 1166
Size: 1653 4429
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 3825 5397 3825
Size: 1572 4510
ApaLI G'TGCA_C
Cuts at: 4139 5295 4139
Size: 1156 4926
ApoI r'AATT_y
Cuts at: 1493 2231 2788 3391 3617 3769 5878 1493
Size: 738 557 603 226 152 2109 1697
Fragments arranged by size:
2109 1697 738 603 557 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 3219 3407 1870
Size: 73 1276 188 4545
Fragments arranged by size:
4545 1276 188 73
BamHI G'GATC_C
Cuts at: 3201 3412 3779 3201
Size: 211 367 5504
Fragments arranged by size:
5504 367 211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 3403 4858 618
Size: 325 217 627 263 1353 1455 1842
Fragments arranged by size:
1842 1455 1353 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2568 3401 1391
Size: 1177 833 4072
Fragments arranged by size:
4072 1177 833
BbeI G_GCGC'C
Cuts at: 2054 2054
Size: 6082
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BbvCI CC'TCA_GC
Cuts at: 3261 3261
Size: 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3095 4693 136
Size: 107 122 71 631 605 1423 1598 1525
Fragments arranged by size:
1598 1525 1423 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2425 6077 2425
Size: 3652 2430
BlpI GC'TnA_GC
Cuts at: 2490 2727 3499 2490
Size: 237 772 5073
Fragments arranged by size:
5073 772 237
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2837 4143 5299 1790
Size: 339 708 1306 1156 2573
Fragments arranged by size:
2573 1306 1156 708 339
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3813 4771 442
Size: 416 53 83 399 24 2396 958 1753
Fragments arranged by size:
2396 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3310 3378 3310
Size: 68 6014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2194 2636 4742 1239
Size: 955 442 2106 2579
Fragments arranged by size:
2579 2106 955 442
Bpu10I CC'TnA_GC
Cuts at: 3261 3261
Size: 6082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2735 4207 5072 5370 2651
Size: 84 1472 865 298 3363
Fragments arranged by size:
3363 1472 865 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3294 4745 882
Size: 617 1795 1451 2219
Fragments arranged by size:
2219 1795 1451 617
BsaAI yAC'GTr
Cuts at: 493 1204 3873 493
Size: 711 2669 2702
Fragments arranged by size:
2702 2669 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 3889 4271 4906
Size: 53 83 186 936 518 1838 382 635
Cuts at: 4906 275
Size: 1451
Fragments arranged by size:
1838 1451 936 635 518 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4515 4913 5187 5334 5745 4515
Size: 398 274 147 411 4852
Fragments arranged by size:
4852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 1178
Size: 30 165 30 816 30 5011
Fragments arranged by size:
5011 816 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 3278 3281 3344 1186
Size: 844 1248 3 63 3924
Fragments arranged by size:
3924 1248 844 63 3
BseYI C'CCAG_C
Cuts at: 2672 3177 5285 5781 2672
Size: 505 2108 496 2973
Fragments arranged by size:
2973 2108 505 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 3532 4293 5321 1540
Size: 275 186 30 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186 30
BsiHKAI G_wGCw'C
Cuts at: 2568 2816 4143 4228 5299 2568
Size: 248 1327 85 1071 3351
Fragments arranged by size:
3351 1327 1071 248 85
BsmI GAATG_Cn'
Cuts at: 3593 3686 3593
Size: 93 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2568 2816 2837 3401 4143 4228
Size: 399 339 439 248 21 564 742 85
Cuts at: 4228 5299 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 564 439 399 339 248
85 21
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3971 5701 3971
Size: 1730 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2330 3437 844
Size: 1486 1107 3489
Fragments arranged by size:
3489 1486 1107
BsrBI CCG'CTC
Cuts at: 1394 1823 2037 2830 3969 1394
Size: 429 214 793 1139 3507
Fragments arranged by size:
3507 1139 793 429 214
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 4577 4751 65
Size: 1183 1029 2300 174 1396
Fragments arranged by size:
2300 1396 1183 1029 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4726 4913 1700
Size: 110 2916 187 2869
Fragments arranged by size:
2916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 4142 5154 4142
Size: 1012 5070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6082
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstXI CCAn_nnnn'nTGG
Cuts at: 2177 2299 2177
Size: 122 5960
BstYI r'GATC_y
Cuts at: 1972 2425 2599 3201 3412 3779 4164 4181 5633
Size: 453 174 602 211 367 385 17 1452
Cuts at: 5633 5719 5731 6077 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 602 453 385 367 346 211
174 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3295 3295
Size: 6082
BtgI C'CryG_G
Cuts at: 513 1065 2061 513
Size: 552 996 4534
Fragments arranged by size:
4534 996 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2695 3598 4392 4412 2695
Size: 903 794 20 4365
Fragments arranged by size:
4365 903 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2979 3096 3529
Size: 54 1425 50 275 18 1149 117 433
Cuts at: 3529 4418 6047 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1149 889 433 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2430 2790 3077 3332 4012 1353
Size: 1077 360 287 255 680 3423
Fragments arranged by size:
3423 1077 680 360 287 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2463 3065 4669 5043 5189 387
Size: 1064 138 874 602 1604 374 146 1280
Fragments arranged by size:
1604 1280 1064 874 602 374 146 138
EcoICRI GAG'CTC
Cuts at: 2566 2566
Size: 6082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2194 2636 4145 4742 5529 5797
Size: 393 494 68 442 1509 597 787 268
Cuts at: 5797 1239
Size: 1524
Fragments arranged by size:
1524 1509 787 597 494 442 393 268
68
EcoO109I rG'GnC_Cy
Cuts at: 2950 3037 3067 3139 2950
Size: 87 30 72 5893
Fragments arranged by size:
5893 87 72 30
EcoRI G'AATT_C
Cuts at: 2788 3391 3769 2788
Size: 603 378 5101
Fragments arranged by size:
5101 603 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 6082
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 2054 5229 1678
Size: 376 3175 2531
Fragments arranged by size:
3175 2531 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 2151 2183 2219
Size: 32 135 32 489 32 58 32 36
Cuts at: 2219 2251 4723 4755 4797 4829 1373
Size: 32 2472 32 42 32 2626
Fragments arranged by size:
2626 2472 489 135 58 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3426 3672 669
Size: 988 281 1488 246 3079
Fragments arranged by size:
3079 1488 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2868 748
Size: 2120 3962
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KasI G'GCGC_C
Cuts at: 2050 2050
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 10
Size: 54 1425 343 1149 3111
Fragments arranged by size:
3111 1425 1149 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2819 3320 4040 4399 4558 518
Size: 648 1131 522 501 720 359 159 2042
Fragments arranged by size:
2042 1131 720 648 522 501 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2560 3181 3199 3498 4175 5323 5568
Size: 450 504 621 18 299 677 1148 245
Cuts at: 5568 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 621 504 450 299 245
18
NarI GG'CG_CC
Cuts at: 2051 2051
Size: 6082
NcoI C'CATG_G
Cuts at: 513 1065 2061 513
Size: 552 996 4534
Fragments arranged by size:
4534 996 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 3440 4985 1132
Size: 97 2211 1545 2229
Fragments arranged by size:
2229 2211 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6082
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6082
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3139 3139
Size: 6082
PshAI GACnn'nnGTC
Cuts at: 1613 2211 1613
Size: 598 5484
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PstI C_TGCA'G
Cuts at: 830 3147 3434 830
Size: 2317 287 3478
Fragments arranged by size:
3478 2317 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 3181 3181
Size: 6082
SacI G_AGCT'C
Cuts at: 2568 2568
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SapI GCTCTTCn'nnn_
Cuts at: 3332 3332
Size: 6082
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SfcI C'TryA_G
Cuts at: 826 1045 2876 2999 3143 3430 4565 5246 5437
Size: 219 1831 123 144 287 1135 681 191
Cuts at: 5437 826
Size: 1471
Fragments arranged by size:
1831 1471 1135 681 287 219 191 144
123
SfiI GGCCn_nnn'nGGCC
Cuts at: 3095 3095
Size: 6082
SfoI GGC'GCC
Cuts at: 2052 2052
Size: 6082
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3221 3409 1872
Size: 1349 188 4545
Fragments arranged by size:
4545 1349 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 2750 4186 5087 5349 820
Size: 197 926 723 84 1436 901 262 1553
Fragments arranged by size:
1553 1436 926 901 723 262 197 84
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 3005 1517
Size: 1488 4594
StyI C'CwwG_G
Cuts at: 513 1065 2061 2769 3279 3521 513
Size: 552 996 708 510 242 3074
Fragments arranged by size:
3074 996 708 552 510 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2172 4109 4126 4279 4464 4909
Size: 1255 465 127 1937 17 153 185 445
Cuts at: 4909 325
Size: 1498
Fragments arranged by size:
1937 1498 1255 465 445 185 153 127
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 4328 6040
Size: 274 80 33 51 1443 864 1487 1712
Cuts at: 6040 96
Size: 138
Fragments arranged by size:
1712 1487 1443 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6042 6074 6042
Size: 32 6050
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 1316
Size: 843 5239
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2296 2632 3178 2068
Size: 228 336 546 4972
Fragments arranged by size:
4972 546 336 228
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 3219 3407 1870
Size: 1349 188 4545
Fragments arranged by size:
4545 1349 188
XmnI GAAnn'nnTTC
Cuts at: 4211 4211
Size: 6082
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SrfI SwaI