Restriction map for
pFN21AB5004
(Circular) (Six-base) MAPSORT of: pFN21AB5004.seq Check: 7256 from: 1 to: 5851
pFN21AB5004 5851 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2334 3661 278
Size: 53 83 186 936 798 1327 2468
Fragments arranged by size:
2468 1327 936 798 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 3194 1937
Size: 145 1112 4594
Fragments arranged by size:
4594 1112 145
Acc65I G'GTAC_C
Cuts at: 2221 2927 3172 2221
Size: 706 245 4900
Fragments arranged by size:
4900 706 245
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3038 3914 5298 5566 1632
Size: 1406 876 1384 268 1917
Fragments arranged by size:
1917 1406 1384 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 2577 4750 5727 1203
Size: 1374 2173 977 1327
Fragments arranged by size:
2173 1374 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 2127 4580 2127
Size: 2453 3398
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 2066 2378 3594 5166 2066
Size: 312 1216 1572 2751
Fragments arranged by size:
2751 1572 1216 312
ApaI G_GGCC'C
Cuts at: 2040 2386 2040
Size: 346 5505
ApaLI G'TGCA_C
Cuts at: 2021 2141 3908 5064 2021
Size: 120 1767 1156 2808
Fragments arranged by size:
2808 1767 1156 120
ApoI r'AATT_y
Cuts at: 1493 2963 3160 3386 3538 5647 1493
Size: 1470 197 226 152 2109 1697
Fragments arranged by size:
2109 1697 1470 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2358 2385 3176 1870
Size: 73 415 27 791 4545
Fragments arranged by size:
4545 791 415 73 27
AvrII C'CTAG_G
Cuts at: 2410 2410
Size: 5851
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2221 2718 2880 2927 3172
Size: 325 217 627 434 497 162 47 245
Cuts at: 3172 4627 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 497 434 325 245 217
162 47
BanII G_rGCy'C
Cuts at: 1391 2040 2386 3170 1391
Size: 649 346 784 4072
Fragments arranged by size:
4072 784 649 346
BbeI G_GCGC'C
Cuts at: 2722 2884 2722
Size: 162 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 3026 928
Size: 971 219 908 3753
Fragments arranged by size:
3753 971 908 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2686 2720 4042 4076 1903
Size: 34 749 34 1322 34 3678
Fragments arranged by size:
3678 1322 749 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2716 4462 136
Size: 107 122 71 631 605 1044 1746 1525
Fragments arranged by size:
1746 1525 1044 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 3268 3268
Size: 5851
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2040 2145 2386 3912 5068 1790
Size: 235 15 105 241 1526 1156 2573
Fragments arranged by size:
2573 1526 1156 241 235 105 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2560 2685 4511 1239
Size: 809 512 125 1826 2579
Fragments arranged by size:
2579 1826 809 512 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4841 5139 3976
Size: 865 298 4688
Fragments arranged by size:
4688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4514 882
Size: 617 3015 2219
Fragments arranged by size:
3015 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2331 2719 2881 3658
Size: 53 83 186 936 798 388 162 777
Cuts at: 3658 4040 4675 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 798 777 635 388 382 186
162 83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5626
Fragments arranged by size:
5626 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3130 1186
Size: 1944 3907
BseYI C'CCAG_C
Cuts at: 2566 5054 5550 2566
Size: 2488 496 2867
Fragments arranged by size:
2867 2488 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2517 1163
Size: 573 781 4497
Fragments arranged by size:
4497 781 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2605 3301 4062 5090 1540
Size: 275 186 604 696 761 1028 2301
Fragments arranged by size:
2301 1028 761 696 604 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2145 2319 3912 3997 5068 2025
Size: 120 174 1593 85 1071 2808
Fragments arranged by size:
2808 1593 1071 174 120 85
BsmI GAATG_Cn'
Cuts at: 2649 3103 3362 3455 2649
Size: 454 259 93 5045
Fragments arranged by size:
5045 454 259 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3142 3142
Size: 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2040 2145 2319 2386 3170 3912
Size: 399 235 15 105 174 67 784 742
Cuts at: 3912 3997 5068 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 399 235 174 105
85 67 15
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3076 3740 5470 3076
Size: 664 1730 3457
Fragments arranged by size:
3457 1730 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3206 844
Size: 2362 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 1394
Size: 429 1915 3507
Fragments arranged by size:
3507 1915 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2317 4346 4520 65
Size: 1183 1069 2029 174 1396
Fragments arranged by size:
2029 1396 1183 1069 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2790 2877 4495 4682 1700
Size: 110 980 87 1618 187 2869
Fragments arranged by size:
2869 1618 980 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 2424 2516 2757 96
Size: 2328 92 241 3190
Fragments arranged by size:
3190 2328 241 92
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 2566
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846
Size: 1209 367 385 17 1452 86 12 346
Cuts at: 5846 1972
Size: 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2083 2083
Size: 5851
Bsu36I CC'TnA_GG
Cuts at: 2712 2943 2712
Size: 231 5620
BtgI C'CryG_G
Cuts at: 513 1065 2203 3145 513
Size: 552 1138 942 3219
Fragments arranged by size:
3219 1138 942 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2123 2163 3367 4161 4181 2067
Size: 56 40 1204 794 20 3737
Fragments arranged by size:
3737 1204 794 56 40 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2267 2912 4858 809
Size: 507 951 645 1946 1802
Fragments arranged by size:
1946 1802 951 645 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3036 3298 4187
Size: 54 1425 50 275 18 1206 262 889
Cuts at: 4187 5816 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1206 889 275 262 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3781 1353
Size: 2428 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 2468 4438 4812 4958 387
Size: 1064 138 713 166 1970 374 146 1280
Fragments arranged by size:
1970 1280 1064 713 374 166 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2560 2685 3038 3914 4511 5298
Size: 393 416 512 125 353 876 597 787
Cuts at: 5298 5566 1239
Size: 268 1524
Fragments arranged by size:
1524 876 787 597 512 416 393 353
268 125
EcoNI CCTnn'n_nnAGG
Cuts at: 2946 2946
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2037 2304 2382 2037
Size: 267 78 5506
Fragments arranged by size:
5506 267 78
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
EcoRV GAT'ATC
Cuts at: 3082 3082
Size: 5851
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2722 2884 4998 1678
Size: 1044 162 2114 2531
Fragments arranged by size:
2531 2114 1044 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2491 2523 2537
Size: 32 135 32 541 32 346 32 14
Cuts at: 2537 2569 4492 4524 4566 4598 1373
Size: 32 1923 32 42 32 2626
Fragments arranged by size:
2626 1923 541 346 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KasI G'GCGC_C
Cuts at: 2718 2880 2718
Size: 162 5689
KpnI G_GTAC'C
Cuts at: 2225 2931 3176 2225
Size: 706 245 4900
Fragments arranged by size:
4900 706 245
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2150 2649 4965 5149 1305
Size: 845 499 2316 184 2007
Fragments arranged by size:
2316 2007 845 499 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 2799 3809 4168 4327 518
Size: 648 1414 219 1010 359 159 2042
Fragments arranged by size:
2042 1414 1010 648 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 3267 3944 5092 5337 1606
Size: 1661 677 1148 245 2120
Fragments arranged by size:
2120 1661 1148 677 245
NaeI GCC'GGC
Cuts at: 2879 2879
Size: 5851
NarI GG'CG_CC
Cuts at: 2719 2881 2719
Size: 162 5689
NcoI C'CATG_G
Cuts at: 513 1065 3145 513
Size: 552 2080 3219
Fragments arranged by size:
3219 2080 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NgoMIV G'CCGG_C
Cuts at: 2877 2877
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2233 2581 3209 4754 1132
Size: 97 1004 348 628 1545 2229
Fragments arranged by size:
2229 1545 1004 628 348 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 2577 4750 2577
Size: 2173 3678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2502 1108
Size: 1394 4457
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 2775 3421 2775
Size: 646 5205
PspOMI G'GGCC_C
Cuts at: 2036 2382 2036
Size: 346 5505
PstI C_TGCA'G
Cuts at: 830 2062 3203 830
Size: 1232 1141 3478
Fragments arranged by size:
3478 1232 1141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 2058 3199 4334 5015 5206 826
Size: 219 1013 1141 1135 681 191 1471
Fragments arranged by size:
1471 1141 1135 1013 681 219 191
SfoI GGC'GCC
Cuts at: 2720 2882 2720
Size: 162 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 2877 1700
Size: 1177 4674
SmaI CCC'GGG
Cuts at: 1872 2387 3178 1872
Size: 515 791 4545
Fragments arranged by size:
4545 791 515
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3955 4856 5118 820
Size: 197 926 2012 901 262 1553
Fragments arranged by size:
2012 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 2952 1517
Size: 1435 4416
StyI C'CwwG_G
Cuts at: 513 1065 2410 3145 3290 513
Size: 552 1345 735 145 3074
Fragments arranged by size:
3074 1345 735 552 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3878 3895 4048 4233 4678 325
Size: 1255 2298 17 153 185 445 1498
Fragments arranged by size:
2298 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2516 2757
Size: 274 80 33 51 1443 447 92 241
Cuts at: 2757 4097 5809 96
Size: 1340 1712 138
Fragments arranged by size:
1712 1443 1340 447 274 241 138 92
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 2267 1316
Size: 951 4900
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 2735
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 2385 3176 1870
Size: 515 791 4545
Fragments arranged by size:
4545 791 515
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2332 3659 276
Size: 53 83 186 936 798 1327 2468
Fragments arranged by size:
2468 1327 936 798 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI DraIII EcoICRI FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI