Restriction map for
pFN21AB4998
(Circular) (Six-base) MAPSORT of: pFN21AB4998.seq Check: 3618 from: 1 to: 5368
pFN21AB4998 5368 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3178 278
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2480 2711 1937
Size: 543 231 4594
Fragments arranged by size:
4594 543 231
Acc65I G'GTAC_C
Cuts at: 2689 2689
Size: 5368
AclI AA'CG_TT
Cuts at: 1563 3495 3868 1563
Size: 1932 373 3063
Fragments arranged by size:
3063 1932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2664 3431 4815 5083 1632
Size: 1032 767 1384 268 1917
Fragments arranged by size:
1917 1384 1032 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5171
AflIII A'CryG_T
Cuts at: 1203 2130 4267 5244 1203
Size: 927 2137 977 1327
Fragments arranged by size:
2137 1327 977 927
AgeI A'CCGG_T
Cuts at: 4199 4199
Size: 5368
AhdI GACnn_n'nnGTC
Cuts at: 4097 4097
Size: 5368
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5368
AlwNI CAG_nnn'CTG
Cuts at: 3111 4683 3111
Size: 1572 3796
ApaLI G'TGCA_C
Cuts at: 3425 4581 3425
Size: 1156 4212
ApoI r'AATT_y
Cuts at: 1493 2677 2903 3055 5164 1493
Size: 1184 226 152 2109 1697
Fragments arranged by size:
2109 1697 1184 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3922 160
Size: 872 2890 1606
Fragments arranged by size:
2890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
AvaI C'yCGr_G
Cuts at: 1870 1943 2693 1870
Size: 73 750 4545
Fragments arranged by size:
4545 750 73
AvrII C'CTAG_G
Cuts at: 2369 2369
Size: 5368
BamHI G'GATC_C
Cuts at: 2698 3065 2698
Size: 367 5001
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2689 4144 618
Size: 325 217 627 902 1455 1842
Fragments arranged by size:
1842 1455 902 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2687 1391
Size: 1296 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 928
Size: 971 196 4201
Fragments arranged by size:
4201 971 196
BbvCI CC'TCA_GC
Cuts at: 2093 2208 2093
Size: 115 5253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3559 3593 1903
Size: 34 1622 34 3678
Fragments arranged by size:
3678 1622 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 4470 3260
Size: 1210 4158
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3979 136
Size: 107 122 71 631 605 2307 1525
Fragments arranged by size:
2307 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2537 5363 2537
Size: 2826 2542
BlpI GC'TnA_GC
Cuts at: 2785 2785
Size: 5368
Bme1580I G_kGCm'C
Cuts at: 1790 3429 4585 1790
Size: 1639 1156 2573
Fragments arranged by size:
2573 1639 1156
BmgBI CAC'GTC
Cuts at: 1318 2131 1318
Size: 813 4555
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2579 3099 4057
Size: 416 53 83 399 24 1162 520 958
Cuts at: 4057 442
Size: 1753
Fragments arranged by size:
1753 1162 958 520 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2518 4028 1239
Size: 1279 1510 2579
Fragments arranged by size:
2579 1510 1279
Bpu10I CC'TnA_GC
Cuts at: 2093 2208 2093
Size: 115 5253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 3493 4358 4656 2036
Size: 1457 865 298 2748
Fragments arranged by size:
2748 1457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2421 2536 2568 4031 882
Size: 617 922 115 32 1463 2219
Fragments arranged by size:
2219 1463 922 617 115 32
BsaAI yAC'GTr
Cuts at: 493 1204 3159 493
Size: 711 1955 2702
Fragments arranged by size:
2702 1955 711
BsaBI GATnn'nnATC
Cuts at: 5362 5362
Size: 5368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2353 2377 3175 3557
Size: 53 83 186 936 820 24 798 382
Cuts at: 3557 4192 275
Size: 635 1451
Fragments arranged by size:
1451 936 820 798 635 382 186 83
53 24
BsaWI w'CCGG_w
Cuts at: 3801 4199 4473 4620 5031 3801
Size: 398 274 147 411 4138
Fragments arranged by size:
4138 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5143
Fragments arranged by size:
5143 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5368
BseYI C'CCAG_C
Cuts at: 4571 5067 4571
Size: 496 4872
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4795
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2251 2284 2480 2621 2818
Size: 275 186 16 234 33 196 141 197
Cuts at: 2818 3579 4607 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 275 234 197 196 186
141 33 16
BsiHKAI G_wGCw'C
Cuts at: 3429 3514 4585 3429
Size: 85 1071 4212
Fragments arranged by size:
4212 1071 85
BsmI GAATG_Cn'
Cuts at: 2879 2972 2879
Size: 93 5275
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 3429 3514 4585 1391
Size: 399 897 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 399 85
BspEI T'CCGG_A
Cuts at: 5031 5031
Size: 5368
BspHI T'CATG_A
Cuts at: 3257 4987 3257
Size: 1730 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2723 844
Size: 1475 404 3489
Fragments arranged by size:
3489 1475 404
BsrBI CCG'CTC
Cuts at: 1394 1823 2442 2657 3255 1394
Size: 429 619 215 598 3507
Fragments arranged by size:
3507 619 598 429 215
BsrDI GCAATG_nn'
Cuts at: 65 1248 3863 4037 65
Size: 1183 2615 174 1396
Fragments arranged by size:
2615 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4012 4199 1700
Size: 110 2202 187 2869
Fragments arranged by size:
2869 2202 187 110
BsrGI T'GTAC_A
Cuts at: 96 2133 96
Size: 2037 3331
BssHII G'CGCG_C
Cuts at: 1925 2273 1925
Size: 348 5020
BssSI C'ACGA_G
Cuts at: 3428 4440 3428
Size: 1012 4356
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5368
BstBI TT'CG_AA
Cuts at: 4172 4172
Size: 5368
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5368
BstYI r'GATC_y
Cuts at: 1972 2495 2537 2698 3065 3450 3467 4919 5005
Size: 523 42 161 367 385 17 1452 86
Cuts at: 5005 5017 5363 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 523 385 367 346 161 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2141 2167 2226 513
Size: 552 1076 26 59 3655
Fragments arranged by size:
3655 1076 552 59 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4629
Fragments arranged by size:
4629 683 56
BtsI GCAGTG_nn'
Cuts at: 2884 3678 3698 2884
Size: 794 20 4554
Fragments arranged by size:
4554 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5333
DraI TTT'AAA
Cuts at: 2672 2672
Size: 5368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2488 4375 809
Size: 507 1172 1887 1802
Fragments arranged by size:
1887 1802 1172 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2248 2281
Size: 54 1425 50 275 18 184 234 33
Cuts at: 2281 2815 3704 5333 8
Size: 534 889 1629 43
Fragments arranged by size:
1629 1425 889 534 275 234 184 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2014 2248 2281 2815 1537
Size: 275 202 234 33 534 4090
Fragments arranged by size:
4090 534 275 234 202 33
EarI CTCTTCn'nnn_
Cuts at: 1353 2618 2640 3298 1353
Size: 1265 22 658 3423
Fragments arranged by size:
3423 1265 658 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3955 4329 4475 387
Size: 1064 138 2366 374 146 1280
Fragments arranged by size:
2366 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2518 2664 3431 4028 4815 5083 1239
Size: 393 886 146 767 597 787 268 1524
Fragments arranged by size:
1524 886 787 767 597 393 268 146
EcoO109I rG'GnC_Cy
Cuts at: 2556 2556
Size: 5368
EcoRI G'AATT_C
Cuts at: 2677 3055 2677
Size: 378 4990
FspI TGC'GCA
Cuts at: 3874 3874
Size: 5368
HaeII r_GCGC'y
Cuts at: 1678 4515 1678
Size: 2837 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4009 4041 4083 4115 1373
Size: 32 135 32 2437 32 42 32 2626
Fragments arranged by size:
2626 2437 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 2712 2958 669
Size: 988 281 543 231 246 3079
Fragments arranged by size:
3079 988 543 281 246 231
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5368
HpaI GTT'AAC
Cuts at: 2958 2958
Size: 5368
KpnI G_GTAC'C
Cuts at: 2693 2693
Size: 5368
MfeI C'AATT_G
Cuts at: 2967 2967
Size: 5368
MluI A'CGCG_T
Cuts at: 5244 5244
Size: 5368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2381 4482 4666 1305
Size: 1076 2101 184 2007
Fragments arranged by size:
2101 2007 1076 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3546
Fragments arranged by size:
3546 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3326 3685 3844 518
Size: 648 2160 359 159 2042
Fragments arranged by size:
2160 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2169 2784 3461 4609 4854 1606
Size: 537 26 615 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 615 537 245 26
NcoI C'CATG_G
Cuts at: 513 1065 2226 513
Size: 552 1161 3655
Fragments arranged by size:
3655 1161 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5368
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5368
NotI GC'GGCC_GC
Cuts at: 2815 2815
Size: 5368
NruI TCG'CGA
Cuts at: 2617 2617
Size: 5368
NspI r_CATG'y
Cuts at: 1132 1229 2726 4271 1132
Size: 97 1497 1545 2229
Fragments arranged by size:
2229 1545 1497 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5368
PciI A'CATG_T
Cuts at: 4267 4267
Size: 5368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5368
PfoI T'CCnGG_A
Cuts at: 2393 2570 2393
Size: 177 5191
PmeI GTTT'AAAC
Cuts at: 2672 2672
Size: 5368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4950 4982 3437
Size: 32 1481 32 3823
Fragments arranged by size:
3823 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2556 2556
Size: 5368
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5368
PsiI TTA'TAA
Cuts at: 2938 2938
Size: 5368
PstI C_TGCA'G
Cuts at: 830 2720 830
Size: 1890 3478
PvuI CG_AT'CG
Cuts at: 2001 2621 2001
Size: 620 4748
SacII CC_GC'GG
Cuts at: 2144 2170 2144
Size: 26 5342
SalI G'TCGA_C
Cuts at: 1936 2479 2710 1936
Size: 543 231 4594
Fragments arranged by size:
4594 543 231
SbfI CC_TGCA'GG
Cuts at: 2720 2720
Size: 5368
ScaI AGT'ACT
Cuts at: 3616 5328 3616
Size: 1712 3656
SfcI C'TryA_G
Cuts at: 826 1045 2434 2716 3851 4532 4723 826
Size: 219 1389 282 1135 681 191 1471
Fragments arranged by size:
1471 1389 1135 681 282 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5368
SmaI CCC'GGG
Cuts at: 1872 2695 1872
Size: 823 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 3472 4373 4635 820
Size: 197 926 108 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 262 197 108
SnaBI TAC'GTA
Cuts at: 493 3159 493
Size: 2666 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5368
SphI G_CATG'C
Cuts at: 1132 2726 1132
Size: 1594 3774
SspI AAT'ATT
Cuts at: 5 52 3292 5
Size: 47 3240 2081
Fragments arranged by size:
3240 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5368
StyI C'CwwG_G
Cuts at: 513 1065 2157 2226 2369 2807 513
Size: 552 1092 69 143 438 3074
Fragments arranged by size:
3074 1092 552 438 143 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2442 2466 2518 2556 3395 3412 3565
Size: 1255 862 24 52 38 839 17 153
Cuts at: 3565 3750 4195 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 862 839 445 185 153 52
38 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 3614 5326
Size: 274 80 33 51 1443 156 1481 1712
Cuts at: 5326 96
Size: 138
Fragments arranged by size:
1712 1481 1443 274 156 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5328 5360 5328
Size: 32 5336
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5368
XbaI T'CTAG_A
Cuts at: 2704 2704
Size: 5368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5368
XmaI C'CCGG_G
Cuts at: 1870 2693 1870
Size: 823 4545
XmnI GAAnn'nnTTC
Cuts at: 3497 3497
Size: 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3176 276
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI