Restriction map for
pFN21AB4990
(Circular) (Six-base) MAPSORT of: pFN21AB4990.seq Check: 206 from: 1 to: 6184
pFN21AB4990 6184 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3505 3505
Size: 6184
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3266 4247 5631 5899 1632
Size: 1634 981 1384 268 1917
Fragments arranged by size:
1917 1634 1384 981 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 6184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2957 1184
Size: 1773 4411
AlwNI CAG_nnn'CTG
Cuts at: 2266 3479 3927 5499 2266
Size: 1213 448 1572 2951
Fragments arranged by size:
2951 1572 1213 448
ApaI G_GGCC'C
Cuts at: 2023 2860 2023
Size: 837 5347
ApaLI G'TGCA_C
Cuts at: 4241 5397 4241
Size: 1156 5028
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2400 3509 1870
Size: 73 457 1109 4545
Fragments arranged by size:
4545 1109 457 73
BamHI G'GATC_C
Cuts at: 2404 2668 3514 3881 2404
Size: 264 846 367 4707
Fragments arranged by size:
4707 846 367 264
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2887 3026 3505 4960 618
Size: 325 217 627 1100 139 479 1455 1842
Fragments arranged by size:
1842 1455 1100 627 479 325 217 139
BanII G_rGCy'C
Cuts at: 1391 2023 2244 2397 2769 2860 2951 3503 1391
Size: 632 221 153 372 91 91 552 4072
Fragments arranged by size:
4072 632 552 372 221 153 91 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3254 928
Size: 971 1355 3858
Fragments arranged by size:
3858 1355 971
BbvCI CC'TCA_GC
Cuts at: 2359 2364 2912 2359
Size: 5 548 5631
Fragments arranged by size:
5631 548 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3195 3229 4375 4409 1903
Size: 34 1258 34 1146 34 3678
Fragments arranged by size:
3678 1258 1146 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6184
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3134 4795 136
Size: 107 122 71 631 605 1462 1661 1525
Fragments arranged by size:
1661 1525 1462 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2553 6179 2553
Size: 3626 2558
BlpI GC'TnA_GC
Cuts at: 2945 3601 2945
Size: 656 5528
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2860 2892 4245 5401 1790
Size: 233 837 32 1353 1156 2573
Fragments arranged by size:
2573 1353 1156 837 233 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3915 4873 442
Size: 416 53 83 399 24 2498 958 1753
Fragments arranged by size:
2498 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3051 1056
Size: 1995 4189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2150 2162 2411 2440 2761 2999 3230
Size: 878 33 12 249 29 321 238 231
Cuts at: 3230 4844 1239
Size: 1614 2579
Fragments arranged by size:
2579 1614 878 321 249 238 231 33
29 12
Bpu10I CC'TnA_GC
Cuts at: 2359 2364 2912 2359
Size: 5 548 5631
Fragments arranged by size:
5631 548 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4309 5174 5472 4309
Size: 865 298 5021
Fragments arranged by size:
5021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3114 3449 3991 4373
Size: 53 83 186 936 1581 335 542 382
Cuts at: 4373 5008 275
Size: 635 1451
Fragments arranged by size:
1581 1451 936 635 542 382 335 186
83 53
BsaWI w'CCGG_w
Cuts at: 2158 2307 4617 5015 5289 5436 5847 2158
Size: 149 2310 398 274 147 411 2495
Fragments arranged by size:
2495 2310 411 398 274 149 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5959
Fragments arranged by size:
5959 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2531 1186
Size: 1345 4839
BseYI C'CCAG_C
Cuts at: 2769 2950 5387 5883 2769
Size: 181 2437 496 3070
Fragments arranged by size:
3070 2437 496 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2992 3189 3326 1163
Size: 573 1256 197 137 4021
Fragments arranged by size:
4021 1256 573 197 137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2397 4245 4330 5401 2244
Size: 153 1848 85 1071 3027
Fragments arranged by size:
3027 1848 1071 153 85
BsmI GAATG_Cn'
Cuts at: 2466 3695 3788 2466
Size: 1229 93 4862
Fragments arranged by size:
4862 1229 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3125 3125
Size: 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2244 2397 2769 2860 2892 2951
Size: 399 233 221 153 372 91 32 59
Cuts at: 2951 3503 4245 4330 5401 1391
Size: 552 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 372 233 221
153 91 85 59 32
BspEI T'CCGG_A
Cuts at: 2158 2307 5847 2158
Size: 149 3540 2495
Fragments arranged by size:
3540 2495 149
BspHI T'CATG_A
Cuts at: 2661 4073 5803 2661
Size: 1412 1730 3042
Fragments arranged by size:
3042 1730 1412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 3168 3180 3539 844
Size: 1328 996 12 359 3489
Fragments arranged by size:
3489 1328 996 359 12
BsrBI CCG'CTC
Cuts at: 1394 1823 2788 3289 4071 1394
Size: 429 965 501 782 3507
Fragments arranged by size:
3507 965 782 501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3057 4679 4853 65
Size: 1183 1809 1622 174 1396
Fragments arranged by size:
1809 1622 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3429 4828 5015 1700
Size: 110 1619 1399 187 2869
Fragments arranged by size:
2869 1619 1399 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 4244 5256 4244
Size: 1012 5172
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2869 794
Size: 2075 4109
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstXI CCAn_nnnn'nTGG
Cuts at: 2095 2692 3064 2095
Size: 597 372 5215
Fragments arranged by size:
5215 597 372
BstYI r'GATC_y
Cuts at: 1972 2073 2404 2553 2668 3309 3514 3881 4266
Size: 101 331 149 115 641 205 367 385
Cuts at: 4266 4283 5735 5821 5833 6179 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 641 385 367 346 331 205
149 115 101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2524 3084 3321 2524
Size: 560 237 5387
Fragments arranged by size:
5387 560 237
BtgI C'CryG_G
Cuts at: 513 1065 2004 2832 513
Size: 552 939 828 3865
Fragments arranged by size:
3865 939 828 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 2766 2961 3042 3453 3700 4494 4514 2766
Size: 195 81 411 247 794 20 4436
Fragments arranged by size:
4436 794 411 247 195 81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2908 2943 529
Size: 35 2344 35 3770
Fragments arranged by size:
3770 2344 35 35
DraI TTT'AAA
Cuts at: 3488 3488
Size: 6184
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3631 4520 6149
Size: 54 1425 50 275 18 1801 889 1629
Cuts at: 6149 8
Size: 43
Fragments arranged by size:
1801 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2030 2778 4114 1353
Size: 665 12 748 1336 3423
Fragments arranged by size:
3423 1336 748 665 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2242 2395 2242
Size: 153 6031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2150 2162 2411 2440 2761 2999
Size: 393 485 33 12 249 29 321 238
Cuts at: 2999 3230 3266 4247 4844 5631 5899 1239
Size: 231 36 981 597 787 268 1524
Fragments arranged by size:
1524 981 787 597 485 393 321 268
249 238 231 36 33 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2663 2663
Size: 6184
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2712 2856 2857 2989 3151 3238 2019
Size: 1 692 144 1 132 162 87 4965
Fragments arranged by size:
4965 692 162 144 132 87 1 1
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FspI TGC'GCA
Cuts at: 2621 3158 3176 4690 2621
Size: 537 18 1514 4115
Fragments arranged by size:
4115 1514 537 18
HaeII r_GCGC'y
Cuts at: 1678 2036 2096 3129 5331 1678
Size: 358 60 1033 2202 2531
Fragments arranged by size:
2531 2202 1033 358 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 2357 2389 2492
Size: 32 135 32 732 32 21 32 103
Cuts at: 2492 2524 4825 4857 4899 4931 1373
Size: 32 2301 32 42 32 2626
Fragments arranged by size:
2626 2301 732 135 103 42 32 32
32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 3528 3774 669
Size: 988 281 1590 246 3079
Fragments arranged by size:
3079 1590 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3509 3509
Size: 6184
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3219 5298 5482 1305
Size: 1914 2079 184 2007
Fragments arranged by size:
2079 2007 1914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4362
Fragments arranged by size:
4362 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3107 4142 4501 4660 518
Size: 648 1941 1035 359 159 2042
Fragments arranged by size:
2042 1941 1035 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2317 2363 2411 2773 2866 3094 3166 3479
Size: 711 46 48 362 93 228 72 313
Cuts at: 3479 3600 4277 5425 5670 1606
Size: 121 677 1148 245 2120
Fragments arranged by size:
2120 1148 711 677 362 313 245 228
121 93 72 48 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 2832 513
Size: 552 939 828 3865
Fragments arranged by size:
3865 939 828 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 3047 1052
Size: 1995 4189
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 2054 2908 3542 5087 1132
Size: 97 825 854 634 1545 2229
Fragments arranged by size:
2229 1545 854 825 634 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2899 1108
Size: 1791 4393
PfoI T'CCnGG_A
Cuts at: 2601 2601
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2989 2989
Size: 6184
PshAI GACnn'nnGTC
Cuts at: 1613 3117 1613
Size: 1504 4680
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspOMI G'GGCC_C
Cuts at: 2019 2856 2019
Size: 837 5347
PstI C_TGCA'G
Cuts at: 830 2778 2865 3261 3536 830
Size: 1948 87 396 275 3478
Fragments arranged by size:
3478 1948 396 275 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2317 2363 2773 2866 3094 3166 3479 2317
Size: 46 410 93 228 72 313 5022
Fragments arranged by size:
5022 410 313 228 93 72 46
SacI G_AGCT'C
Cuts at: 2244 2397 2244
Size: 153 6031
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SapI GCTCTTCn'nnn_
Cuts at: 2778 2778
Size: 6184
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2774 2861 3257 3532 4667 5348 5539
Size: 219 1729 87 396 275 1135 681 191
Cuts at: 5539 826
Size: 1471
Fragments arranged by size:
1729 1471 1135 681 396 275 219 191
87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 2402 3511 1872
Size: 530 1109 4545
Fragments arranged by size:
4545 1109 530
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4288 5189 5451 820
Size: 197 926 2345 901 262 1553
Fragments arranged by size:
2345 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 4108 5
Size: 47 4056 2081
Fragments arranged by size:
4056 2081 47
StuI AGG'CCT
Cuts at: 1517 2447 1517
Size: 930 5254
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2292 2832 3623 513
Size: 552 939 84 204 540 791 3074
Fragments arranged by size:
3074 939 791 552 540 204 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4211 4228 4381 4566 5011 325
Size: 1255 2631 17 153 185 445 1498
Fragments arranged by size:
2631 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3283 3315 6144 6176 3283
Size: 32 2829 32 3291
Fragments arranged by size:
3291 2829 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2689 2758 3106 2290
Size: 399 69 348 5368
Fragments arranged by size:
5368 399 348 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6184
XmaI C'CCGG_G
Cuts at: 1870 2400 3509 1870
Size: 530 1109 4545
Fragments arranged by size:
4545 1109 530
XmnI GAAnn'nnTTC
Cuts at: 3190 4313 3190
Size: 1123 5061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI