Restriction map for
pFN21AB4985
(Circular) (Six-base) MAPSORT of: pFN21AB4985.seq Check: 7731 from: 1 to: 6310
pFN21AB4985 6310 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2948 2948
Size: 6310
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4120 278
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3653 1937
Size: 1716 4594
Acc65I G'GTAC_C
Cuts at: 3631 3631
Size: 6310
AclI AA'CG_TT
Cuts at: 1563 4437 4810 1563
Size: 2874 373 3063
Fragments arranged by size:
3063 2874 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3549 4373 5757 6025 1632
Size: 1917 824 1384 268 1917
Fragments arranged by size:
1917 1917 1384 824 268
AflII C'TTAA_G
Cuts at: 820 1017 2852 820
Size: 197 1835 4278
Fragments arranged by size:
4278 1835 197
AflIII A'CryG_T
Cuts at: 1203 3586 5209 6186 1203
Size: 2383 1623 977 1327
Fragments arranged by size:
2383 1623 1327 977
AgeI A'CCGG_T
Cuts at: 5141 5141
Size: 6310
AhdI GACnn_n'nnGTC
Cuts at: 2076 5039 2076
Size: 2963 3347
AleI CACnn'nnGTG
Cuts at: 1166 3606 1166
Size: 2440 3870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6310
AlwNI CAG_nnn'CTG
Cuts at: 2051 4053 5625 2051
Size: 2002 1572 2736
Fragments arranged by size:
2736 2002 1572
ApaLI G'TGCA_C
Cuts at: 4367 5523 4367
Size: 1156 5154
ApoI r'AATT_y
Cuts at: 1493 2154 2465 2712 3147 3619 3845 3997 6106
Size: 661 311 247 435 472 226 152 2109
Cuts at: 6106 1493
Size: 1697
Fragments arranged by size:
2109 1697 661 472 435 311 247 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2888 4864 160
Size: 872 1856 1976 1606
Fragments arranged by size:
1976 1856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
AvaI C'yCGr_G
Cuts at: 1870 1943 2766 3635 1870
Size: 73 823 869 4545
Fragments arranged by size:
4545 869 823 73
BamHI G'GATC_C
Cuts at: 3640 4007 3640
Size: 367 5943
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2204 3134 3452 3574
Size: 325 217 627 309 108 930 318 122
Cuts at: 3574 3631 5086 618
Size: 57 1455 1842
Fragments arranged by size:
1842 1455 930 627 325 318 309 217
122 108 57
BanII G_rGCy'C
Cuts at: 1391 3629 1391
Size: 2238 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3078 928
Size: 971 1179 4160
Fragments arranged by size:
4160 1179 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3031 3065 4501 4535 1903
Size: 34 1094 34 1436 34 3678
Fragments arranged by size:
3678 1436 1094 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2731 4202 5412 2731
Size: 1471 1210 3629
Fragments arranged by size:
3629 1471 1210
BclI T'GATC_A
Cuts at: 1548 2443 3010 3595 1548
Size: 895 567 585 4263
Fragments arranged by size:
4263 895 585 567
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2408 4921 136
Size: 107 122 71 631 605 736 2513 1525
Fragments arranged by size:
2513 1525 736 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6305 6305
Size: 6310
BlpI GC'TnA_GC
Cuts at: 3727 3727
Size: 6310
Bme1580I G_kGCm'C
Cuts at: 1790 2498 4371 5527 1790
Size: 708 1873 1156 2573
Fragments arranged by size:
2573 1873 1156 708
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4041 4999 442
Size: 416 53 83 399 24 2624 958 1753
Fragments arranged by size:
2624 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 4970 1239
Size: 1373 2358 2579
Fragments arranged by size:
2579 2358 1373
Bpu10I CC'TnA_GC
Cuts at: 3398 3398
Size: 6310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4435 5300 5598 4435
Size: 865 298 5147
Fragments arranged by size:
5147 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4973 882
Size: 617 3474 2219
Fragments arranged by size:
3474 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4101 493
Size: 711 2897 2702
Fragments arranged by size:
2897 2702 711
BsaBI GATnn'nnATC
Cuts at: 6304 6304
Size: 6310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4117 4499 5134
Size: 53 83 186 936 476 2108 382 635
Cuts at: 5134 275
Size: 1451
Fragments arranged by size:
2108 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4743 5141 5415 5562 5973 4743
Size: 398 274 147 411 5080
Fragments arranged by size:
5080 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2103 2133 1178
Size: 30 165 30 700 30 5355
Fragments arranged by size:
5355 700 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3246 1186
Size: 2060 4250
BseYI C'CCAG_C
Cuts at: 5513 6009 5513
Size: 496 5814
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3399 3543 1163
Size: 573 1663 144 3930
Fragments arranged by size:
3930 1663 573 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3760 4521 5549 1540
Size: 275 186 1759 761 1028 2301
Fragments arranged by size:
2301 1759 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2129 4371 4456 5527 2129
Size: 2242 85 1071 2912
Fragments arranged by size:
2912 2242 1071 85
BsmI GAATG_Cn'
Cuts at: 3821 3914 3821
Size: 93 6217
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2498 3629 4371 4456 5527 1391
Size: 399 339 369 1131 742 85 1071 2174
Fragments arranged by size:
2174 1131 1071 742 399 369 339 85
BspEI T'CCGG_A
Cuts at: 5973 5973
Size: 6310
BspHI T'CATG_A
Cuts at: 3060 4199 5929 3060
Size: 1139 1730 3441
Fragments arranged by size:
3441 1730 1139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2948 3665 844
Size: 2104 717 3489
Fragments arranged by size:
3489 2104 717
BsrBI CCG'CTC
Cuts at: 1394 1823 4197 1394
Size: 429 2374 3507
Fragments arranged by size:
3507 2374 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3170 3315 4805 4979 65
Size: 1183 1922 145 1490 174 1396
Fragments arranged by size:
1922 1490 1396 1183 174 145
BsrFI r'CCGG_y
Cuts at: 1700 1810 4954 5141 1700
Size: 110 3144 187 2869
Fragments arranged by size:
3144 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6310
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6310
BssSI C'ACGA_G
Cuts at: 4370 5382 4370
Size: 1012 5298
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2795 2954 794
Size: 2001 159 4150
Fragments arranged by size:
4150 2001 159
BstBI TT'CG_AA
Cuts at: 5114 5114
Size: 6310
BstEII G'GTnAC_C
Cuts at: 1184 2191 1184
Size: 1007 5303
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 6310
BstYI r'GATC_y
Cuts at: 1972 3640 4007 4392 4409 5861 5947 5959 6305
Size: 1668 367 385 17 1452 86 12 346
Cuts at: 6305 1972
Size: 1977
Fragments arranged by size:
1977 1668 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4819
Fragments arranged by size:
4819 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2086 487
Size: 683 56 860 4711
Fragments arranged by size:
4711 860 683 56
BtsI GCAGTG_nn'
Cuts at: 2173 3426 3826 4620 4640 2173
Size: 1253 400 794 20 3843
Fragments arranged by size:
3843 1253 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6275
DraI TTT'AAA
Cuts at: 3614 3614
Size: 6310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5317 809
Size: 507 4001 1802
Fragments arranged by size:
4001 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3757 4646 6275
Size: 54 1425 50 275 18 1927 889 1629
Cuts at: 6275 8
Size: 43
Fragments arranged by size:
1927 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3757 1537
Size: 275 1945 4090
Fragments arranged by size:
4090 1945 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 4240 1353
Size: 699 2188 3423
Fragments arranged by size:
3423 2188 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4897 5271 5417 387
Size: 1064 138 3308 374 146 1280
Fragments arranged by size:
3308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2612 3549 4373 4970 5757 6025 1239
Size: 393 980 937 824 597 787 268 1524
Fragments arranged by size:
1524 980 937 824 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3107 3107
Size: 6310
EcoO109I rG'GnC_Cy
Cuts at: 2135 2135
Size: 6310
EcoRI G'AATT_C
Cuts at: 3619 3997 3619
Size: 378 5932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 6310
FspI TGC'GCA
Cuts at: 3198 4816 3198
Size: 1618 4692
HaeII r_GCGC'y
Cuts at: 1678 2426 5457 1678
Size: 748 3031 2531
Fragments arranged by size:
3031 2531 748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2062 2094 4951 4983 5025
Size: 32 135 32 490 32 2857 32 42
Cuts at: 5025 5057 1373
Size: 32 2626
Fragments arranged by size:
2857 2626 490 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2032 2233 2779 3654 3900 669
Size: 988 281 94 201 546 875 246 3079
Fragments arranged by size:
3079 988 875 546 281 246 201 94
HindIII A'AGCT_T
Cuts at: 748 3029 748
Size: 2281 4029
HpaI GTT'AAC
Cuts at: 2032 3900 2032
Size: 1868 4442
KpnI G_GTAC'C
Cuts at: 3635 3635
Size: 6310
MfeI C'AATT_G
Cuts at: 3909 3909
Size: 6310
MluI A'CGCG_T
Cuts at: 6186 6186
Size: 6310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2372 2643 5424 5608 1305
Size: 1067 271 2781 184 2007
Fragments arranged by size:
2781 2007 1067 271 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4488
Fragments arranged by size:
4488 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3606 4268 4627 4786 518
Size: 648 2440 662 359 159 2042
Fragments arranged by size:
2440 2042 662 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3527 3726 4403 5551 5796 1606
Size: 1921 199 677 1148 245 2120
Fragments arranged by size:
2120 1921 1148 677 245 199
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4819
Fragments arranged by size:
4819 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6310
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6310
NotI GC'GGCC_GC
Cuts at: 3757 3757
Size: 6310
NspI r_CATG'y
Cuts at: 1132 1229 2813 2948 3668 5213 1132
Size: 97 1584 135 720 1545 2229
Fragments arranged by size:
2229 1584 1545 720 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6310
PciI A'CATG_T
Cuts at: 5209 5209
Size: 6310
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6310
PmeI GTTT'AAAC
Cuts at: 3614 3614
Size: 6310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 5892 5924 4379
Size: 32 1481 32 4765
Fragments arranged by size:
4765 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2135 2135
Size: 6310
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6310
PsiI TTA'TAA
Cuts at: 2895 3880 2895
Size: 985 5325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2547 2579 2547
Size: 32 6278
PstI C_TGCA'G
Cuts at: 830 2440 3662 830
Size: 1610 1222 3478
Fragments arranged by size:
3478 1610 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6310
PvuII CAG'CTG
Cuts at: 3527 3527
Size: 6310
SalI G'TCGA_C
Cuts at: 1936 3652 1936
Size: 1716 4594
SanDI GG'GwC_CC
Cuts at: 2135 2135
Size: 6310
SbfI CC_TGCA'GG
Cuts at: 3662 3662
Size: 6310
ScaI AGT'ACT
Cuts at: 4558 6270 4558
Size: 1712 4598
SexAI A'CCwGG_T
Cuts at: 3384 3384
Size: 6310
SfcI C'TryA_G
Cuts at: 826 1045 2436 2553 3658 4793 5474 5665 826
Size: 219 1391 117 1105 1135 681 191 1471
Fragments arranged by size:
1471 1391 1135 1105 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6310
SmaI CCC'GGG
Cuts at: 1872 2768 3637 1872
Size: 896 869 4545
Fragments arranged by size:
4545 896 869
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2852 4414 5315 5577 820
Size: 197 926 909 1562 901 262 1553
Fragments arranged by size:
1562 1553 926 909 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4101 493
Size: 3608 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6310
SphI G_CATG'C
Cuts at: 1132 2948 3668 1132
Size: 1816 720 3774
Fragments arranged by size:
3774 1816 720
SspI AAT'ATT
Cuts at: 5 52 2488 4234 5
Size: 47 2436 1746 2081
Fragments arranged by size:
2436 2081 1746 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 3103 3749 513
Size: 552 939 126 973 646 3074
Fragments arranged by size:
3074 973 939 646 552 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3030 3592 4337 4354 4507 4692 5137
Size: 1255 1450 562 745 17 153 185 445
Cuts at: 5137 325
Size: 1498
Fragments arranged by size:
1498 1450 1255 745 562 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4556 6268 96
Size: 274 80 33 51 1443 2579 1712 138
Fragments arranged by size:
2579 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6270 6302 6270
Size: 32 6278
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6310
XbaI T'CTAG_A
Cuts at: 3215 3646 3215
Size: 431 5879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 3433 3463 2113
Size: 1320 30 4960
Fragments arranged by size:
4960 1320 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6310
XmaI C'CCGG_G
Cuts at: 1870 2766 3635 1870
Size: 896 869 4545
Fragments arranged by size:
4545 896 869
XmnI GAAnn'nnTTC
Cuts at: 2562 2847 3492 4439 2562
Size: 285 645 947 4433
Fragments arranged by size:
4433 947 645 285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4118 276
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI